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The map label for this gene is nikB [H]

Identifier: 52786547

GI number: 52786547

Start: 2696805

End: 2697749

Strand: Direct

Name: nikB [H]

Synonym: BLi02812

Alternate gene names: 52786547

Gene position: 2696805-2697749 (Clockwise)

Preceding gene: 52786546

Following gene: 52786548

Centisome position: 63.87

GC content: 44.02

Gene sequence:

>945_bases
ATGGGCAACTATATATTGAAACGAATCATGTCGATTATTCCAGTTTTTCTTTTAGCTGCACTTCTGACAACTGGAATGAT
TCATCTCTCGCCGGTTGACCCAGCTGAAGCATACTTAGCCGCAGCGCATATCCAGCCAACTGATGAAATTTTAGCGCAAA
AAAGACATGAGTTTGGCTTGGATCAGCCATTTTTTATTCAATATGTAAACTCTGTTTTGAAGATATGCCAGCTTGATTTT
GGCATATCTTATGTTTCAAACCAGCCGGTTTGGGACGAAGTGGCACACCGAATGCCGGCAACGGTCGAACTTGCGCTTGG
GAGTTTATTGATTGCGGTGCTTGTCAGTGTGCCGCTCGGCTTCTTAGCGGGAATCAAAAGAAATGGTGCAGTTGATCATA
TCAGCCGCTTCATTTCCTTTTTCGGGGCATCCATCCCAACTTTTTGGTTAGGGTATATGCTGGTTTTTTTCTTTTCTGTT
CAATTAGACCTTTTCCCGGTAGAGGGCGCTGGGACATGGCAGCATCTCGTACTGCCGTCTATGACATTGGCGCTTCCTTT
AATTGCGATGTATACAAGGCTGCTCCGTGCAAGCGTCATCGAAAACCTGCAGGAACCATATGTTCAATTTGCACGCATAA
GAGGAATAGCGAAGTTGAACATTATGGCCAAACATGTATTGCGAATGGCCATTTCTCCGATGATTACGGGTCTGGGAATG
AATCTTGGCAAGCTGCTTACCGGCACAATTATTGTTGAAGTCGTTTTTTCTTGGCCCGGCTTTGGCCGTTATTTTATTGA
GGCCATTTTCAATCGGGACATCCCTGTCATTCAATGCTATGTGTTCTTAGCCGCTACCTTTTTTATATTGAGCAACTTAA
TTGTTGATCTCATTCAAATGTATATTGACCCGCGCATTTCAAGGAAAGAAGGGCAGCATCGATGA

Upstream 100 bases:

>100_bases
GATATATCAAAAGAATATCACCGACTTTACGTTCCCTGCTAACCGTGACGAACATCCATTTACAGGCATTCGCGTCAAGC
AGCAACACTAGGAGGCTTTT

Downstream 100 bases:

>100_bases
TGATAAGTATACGCAGGATATTCAAAGGCCAAAAAGTGATTACGGTATGCACCACTATATTAACCATCCTTTTTATTATC
GCTGTATTCGCCCCTTGGAT

Product: hypothetical protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MGNYILKRIMSIIPVFLLAALLTTGMIHLSPVDPAEAYLAAAHIQPTDEILAQKRHEFGLDQPFFIQYVNSVLKICQLDF
GISYVSNQPVWDEVAHRMPATVELALGSLLIAVLVSVPLGFLAGIKRNGAVDHISRFISFFGASIPTFWLGYMLVFFFSV
QLDLFPVEGAGTWQHLVLPSMTLALPLIAMYTRLLRASVIENLQEPYVQFARIRGIAKLNIMAKHVLRMAISPMITGLGM
NLGKLLTGTIIVEVVFSWPGFGRYFIEAIFNRDIPVIQCYVFLAATFFILSNLIVDLIQMYIDPRISRKEGQHR

Sequences:

>Translated_314_residues
MGNYILKRIMSIIPVFLLAALLTTGMIHLSPVDPAEAYLAAAHIQPTDEILAQKRHEFGLDQPFFIQYVNSVLKICQLDF
GISYVSNQPVWDEVAHRMPATVELALGSLLIAVLVSVPLGFLAGIKRNGAVDHISRFISFFGASIPTFWLGYMLVFFFSV
QLDLFPVEGAGTWQHLVLPSMTLALPLIAMYTRLLRASVIENLQEPYVQFARIRGIAKLNIMAKHVLRMAISPMITGLGM
NLGKLLTGTIIVEVVFSWPGFGRYFIEAIFNRDIPVIQCYVFLAATFFILSNLIVDLIQMYIDPRISRKEGQHR
>Mature_313_residues
GNYILKRIMSIIPVFLLAALLTTGMIHLSPVDPAEAYLAAAHIQPTDEILAQKRHEFGLDQPFFIQYVNSVLKICQLDFG
ISYVSNQPVWDEVAHRMPATVELALGSLLIAVLVSVPLGFLAGIKRNGAVDHISRFISFFGASIPTFWLGYMLVFFFSVQ
LDLFPVEGAGTWQHLVLPSMTLALPLIAMYTRLLRASVIENLQEPYVQFARIRGIAKLNIMAKHVLRMAISPMITGLGMN
LGKLLTGTIIVEVVFSWPGFGRYFIEAIFNRDIPVIQCYVFLAATFFILSNLIVDLIQMYIDPRISRKEGQHR

Specific function: Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane [H]

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789888, Length=313, Percent_Identity=45.0479233226837, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI1787053, Length=309, Percent_Identity=38.5113268608414, Blast_Score=224, Evalue=6e-60,
Organism=Escherichia coli, GI1789965, Length=341, Percent_Identity=35.7771260997067, Blast_Score=220, Evalue=9e-59,
Organism=Escherichia coli, GI1787761, Length=335, Percent_Identity=33.4328358208955, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1787550, Length=263, Percent_Identity=32.6996197718631, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1787497, Length=309, Percent_Identity=28.4789644012945, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1788504, Length=360, Percent_Identity=26.1111111111111, Blast_Score=124, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR014156 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35215; Mature: 35084

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNYILKRIMSIIPVFLLAALLTTGMIHLSPVDPAEAYLAAAHIQPTDEILAQKRHEFGL
CCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHCCC
DQPFFIQYVNSVLKICQLDFGISYVSNQPVWDEVAHRMPATVELALGSLLIAVLVSVPLG
CCHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
FLAGIKRNGAVDHISRFISFFGASIPTFWLGYMLVFFFSVQLDLFPVEGAGTWQHLVLPS
HHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHH
MTLALPLIAMYTRLLRASVIENLQEPYVQFARIRGIAKLNIMAKHVLRMAISPMITGLGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NLGKLLTGTIIVEVVFSWPGFGRYFIEAIFNRDIPVIQCYVFLAATFFILSNLIVDLIQM
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YIDPRISRKEGQHR
HHCCCCCCCCCCCC
>Mature Secondary Structure 
GNYILKRIMSIIPVFLLAALLTTGMIHLSPVDPAEAYLAAAHIQPTDEILAQKRHEFGL
CHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHCCC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HHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]