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The map label for this gene is nikA [H]

Identifier: 52786546

GI number: 52786546

Start: 2695173

End: 2696795

Strand: Direct

Name: nikA [H]

Synonym: BLi02811

Alternate gene names: 52786546

Gene position: 2695173-2696795 (Clockwise)

Preceding gene: 52786544

Following gene: 52786547

Centisome position: 63.83

GC content: 42.14

Gene sequence:

>1623_bases
ATGATGAGCGGAAGGATTAGTTTGAAAATTAAAATTATTTTTATATTAATGCTCGCCTTTTCTATTTTATTAGCCGGATG
TACAACCTCAGGAAATTCAACAGAGAAAACTCATGAAAACTTGGTCACAATGGCTTGGCCTAGAGATATAGGCGAAATGA
ATCCGCATGTGTATAATCCTTCCCAATTATTTGCCCAGTCGATGGTATATGAACCTTTGGTTACCTATGAAGCGGGAGGA
AAGCTTAAACCGCATTTAGCCGAATCTTGGGATATATCAAGTGACGGTAAAGTATATACGTTTCATTTGCGTAAGAATGT
AACATTTTCAGATGGAACGAAGTTTGATGCGAAAATTGTGAAAAAGAATTTTGACTCCATTTTAAAACATACTGAATTAC
ATAGCTGGTTAGGGTTTATTTCAAAAATAGCCCAAACAGAAGTGATCGATGACCATACATTTAAATTAAAATTGACTGAA
CCATACTACCCGACCATTCAGGAACTAGCCGTTGTACGGCCGGTCCGGTTTTTGGGAGAAGCGGGCTTCCCTGAGGACGG
CGATACATCAAAGGGTGTCGAAAAACCGGTTGGTACGGGCCCATGGGTATTGGAAGAGCATAAAGCTGATGAATATGCGG
TTTTTAAACGCAATGAACATTATTGGGGTGAAAAGCCAAAAGTCGAGAAAATCAAAGTGAAAGTTATCCCAGATGCCGAA
ACAAGGGTGCTAGCATTTGAAAAAGGCGAGCTTGACCTCATTTACGGCGAAGGCGTTATCAGTTTGGATGCATACAAGCA
GCTGCAATCGACAGGTCAATATGAAACAAGCCTTTCTGAACCCGTGGCGACGAGACAGCTCGTCATGAATACGAAAAAAG
AACAGCTTTCTGATGAGCGCGTGCGGCAGGCCCTGCAATACGGTTTTGACAAAGAAGCCATGGTGAAAGGCATCACCTCA
GGGTTAGAAGAAAAAGCAGATCATATTTTGCCGACAGACTTCCCATACACGTCTGATATTGATGTAAAGCAAATCAACTA
TGATACAGAAAAAGCAAAAGAGTTATTAGATGCGGCCGGCTGGAAGCTTCCGAATGGCAAGACCGTGCGCGAGAAAGACG
GAAAGCCTCTTGAATTTAGTTTAATGTATGATTCAGCTGAATCCATTCAAAAAACAATGGCTGAAACGCTGCAGGCAGAA
TGGGCAGCGATCGGGGTAAAGCTGAATCTTGAAGGCGTAGAATTGGCAACCCAAGTCAAACGGTTTAAGGCAAATGAGTT
TGATATGAACTTCTTTAGCAACTATGGTGCGCCTTATGACCCGCATACATTTTTAAACATTGTCGCGTCCAAAGGATTCG
GATTTAATGAAGCGATCTCAGCCTATCCAAACAAGGACGAGCTAATCAAACAGATGGCAAAGGTGCCTCAAACGACTGAT
GAAAAAGAGCGGCAGGAGCATTACTCATCTATTTTAAAATCACTTCAAGACCAGGGAGCCATCGTACCTGTTTCTTACAT
TAAAAAGACGGCGATATATCAAAAGAATATCACCGACTTTACGTTCCCTGCTAACCGTGACGAACATCCATTTACAGGCA
TTCGCGTCAAGCAGCAACACTAG

Upstream 100 bases:

>100_bases
TCCGCAGCATTTAGATAGTTCCTATATCTTACATTGACAAGGGTATGCCCCCTGCATAAAATGAAAATGATTATCAACTT
CATTTAGGAGGAAACAAAGC

Downstream 100 bases:

>100_bases
GAGGCTTTTATGGGCAACTATATATTGAAACGAATCATGTCGATTATTCCAGTTTTTCTTTTAGCTGCACTTCTGACAAC
TGGAATGATTCATCTCTCGC

Product: hypothetical protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MMSGRISLKIKIIFILMLAFSILLAGCTTSGNSTEKTHENLVTMAWPRDIGEMNPHVYNPSQLFAQSMVYEPLVTYEAGG
KLKPHLAESWDISSDGKVYTFHLRKNVTFSDGTKFDAKIVKKNFDSILKHTELHSWLGFISKIAQTEVIDDHTFKLKLTE
PYYPTIQELAVVRPVRFLGEAGFPEDGDTSKGVEKPVGTGPWVLEEHKADEYAVFKRNEHYWGEKPKVEKIKVKVIPDAE
TRVLAFEKGELDLIYGEGVISLDAYKQLQSTGQYETSLSEPVATRQLVMNTKKEQLSDERVRQALQYGFDKEAMVKGITS
GLEEKADHILPTDFPYTSDIDVKQINYDTEKAKELLDAAGWKLPNGKTVREKDGKPLEFSLMYDSAESIQKTMAETLQAE
WAAIGVKLNLEGVELATQVKRFKANEFDMNFFSNYGAPYDPHTFLNIVASKGFGFNEAISAYPNKDELIKQMAKVPQTTD
EKERQEHYSSILKSLQDQGAIVPVSYIKKTAIYQKNITDFTFPANRDEHPFTGIRVKQQH

Sequences:

>Translated_540_residues
MMSGRISLKIKIIFILMLAFSILLAGCTTSGNSTEKTHENLVTMAWPRDIGEMNPHVYNPSQLFAQSMVYEPLVTYEAGG
KLKPHLAESWDISSDGKVYTFHLRKNVTFSDGTKFDAKIVKKNFDSILKHTELHSWLGFISKIAQTEVIDDHTFKLKLTE
PYYPTIQELAVVRPVRFLGEAGFPEDGDTSKGVEKPVGTGPWVLEEHKADEYAVFKRNEHYWGEKPKVEKIKVKVIPDAE
TRVLAFEKGELDLIYGEGVISLDAYKQLQSTGQYETSLSEPVATRQLVMNTKKEQLSDERVRQALQYGFDKEAMVKGITS
GLEEKADHILPTDFPYTSDIDVKQINYDTEKAKELLDAAGWKLPNGKTVREKDGKPLEFSLMYDSAESIQKTMAETLQAE
WAAIGVKLNLEGVELATQVKRFKANEFDMNFFSNYGAPYDPHTFLNIVASKGFGFNEAISAYPNKDELIKQMAKVPQTTD
EKERQEHYSSILKSLQDQGAIVPVSYIKKTAIYQKNITDFTFPANRDEHPFTGIRVKQQH
>Mature_540_residues
MMSGRISLKIKIIFILMLAFSILLAGCTTSGNSTEKTHENLVTMAWPRDIGEMNPHVYNPSQLFAQSMVYEPLVTYEAGG
KLKPHLAESWDISSDGKVYTFHLRKNVTFSDGTKFDAKIVKKNFDSILKHTELHSWLGFISKIAQTEVIDDHTFKLKLTE
PYYPTIQELAVVRPVRFLGEAGFPEDGDTSKGVEKPVGTGPWVLEEHKADEYAVFKRNEHYWGEKPKVEKIKVKVIPDAE
TRVLAFEKGELDLIYGEGVISLDAYKQLQSTGQYETSLSEPVATRQLVMNTKKEQLSDERVRQALQYGFDKEAMVKGITS
GLEEKADHILPTDFPYTSDIDVKQINYDTEKAKELLDAAGWKLPNGKTVREKDGKPLEFSLMYDSAESIQKTMAETLQAE
WAAIGVKLNLEGVELATQVKRFKANEFDMNFFSNYGAPYDPHTFLNIVASKGFGFNEAISAYPNKDELIKQMAKVPQTTD
EKERQEHYSSILKSLQDQGAIVPVSYIKKTAIYQKNITDFTFPANRDEHPFTGIRVKQQH

Specific function: Involved in a nickel transport system, probably represents the nickel binder [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=495, Percent_Identity=39.7979797979798, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1789966, Length=538, Percent_Identity=28.8104089219331, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1787762, Length=449, Percent_Identity=26.7260579064588, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1787052, Length=538, Percent_Identity=25.4646840148699, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1787495, Length=465, Percent_Identity=27.0967741935484, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI87081878, Length=469, Percent_Identity=26.4392324093817, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787551, Length=299, Percent_Identity=26.0869565217391, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1789397, Length=465, Percent_Identity=23.010752688172, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011980
- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61140; Mature: 61140

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSGRISLKIKIIFILMLAFSILLAGCTTSGNSTEKTHENLVTMAWPRDIGEMNPHVYNP
CCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCEEEEECCCCHHCCCCCCCCH
SQLFAQSMVYEPLVTYEAGGKLKPHLAESWDISSDGKVYTFHLRKNVTFSDGTKFDAKIV
HHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCEECCCCCHHHHHH
KKNFDSILKHTELHSWLGFISKIAQTEVIDDHTFKLKLTEPYYPTIQELAVVRPVRFLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCCHHHHHHHHHHHHHHCC
AGFPEDGDTSKGVEKPVGTGPWVLEEHKADEYAVFKRNEHYWGEKPKVEKIKVKVIPDAE
CCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCCCCCCCCEEEEEEEECCCC
TRVLAFEKGELDLIYGEGVISLDAYKQLQSTGQYETSLSEPVATRQLVMNTKKEQLSDER
CEEEEEECCCEEEEECCCEEEHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCCHHHHHHHH
VRQALQYGFDKEAMVKGITSGLEEKADHILPTDFPYTSDIDVKQINYDTEKAKELLDAAG
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHC
WKLPNGKTVREKDGKPLEFSLMYDSAESIQKTMAETLQAEWAAIGVKLNLEGVELATQVK
CCCCCCCEECCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHH
RFKANEFDMNFFSNYGAPYDPHTFLNIVASKGFGFNEAISAYPNKDELIKQMAKVPQTTD
HHCCCCCCCHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHCCCCCC
EKERQEHYSSILKSLQDQGAIVPVSYIKKTAIYQKNITDFTFPANRDEHPFTGIRVKQQH
HHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEECCC
>Mature Secondary Structure
MMSGRISLKIKIIFILMLAFSILLAGCTTSGNSTEKTHENLVTMAWPRDIGEMNPHVYNP
CCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCEEEEECCCCHHCCCCCCCCH
SQLFAQSMVYEPLVTYEAGGKLKPHLAESWDISSDGKVYTFHLRKNVTFSDGTKFDAKIV
HHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCEECCCCCHHHHHH
KKNFDSILKHTELHSWLGFISKIAQTEVIDDHTFKLKLTEPYYPTIQELAVVRPVRFLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCCHHHHHHHHHHHHHHCC
AGFPEDGDTSKGVEKPVGTGPWVLEEHKADEYAVFKRNEHYWGEKPKVEKIKVKVIPDAE
CCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEECCCCCCCCCCCCEEEEEEEECCCC
TRVLAFEKGELDLIYGEGVISLDAYKQLQSTGQYETSLSEPVATRQLVMNTKKEQLSDER
CEEEEEECCCEEEEECCCEEEHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCCHHHHHHHH
VRQALQYGFDKEAMVKGITSGLEEKADHILPTDFPYTSDIDVKQINYDTEKAKELLDAAG
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHC
WKLPNGKTVREKDGKPLEFSLMYDSAESIQKTMAETLQAEWAAIGVKLNLEGVELATQVK
CCCCCCCEECCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHH
RFKANEFDMNFFSNYGAPYDPHTFLNIVASKGFGFNEAISAYPNKDELIKQMAKVPQTTD
HHCCCCCCCHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHCCCCCC
EKERQEHYSSILKSLQDQGAIVPVSYIKKTAIYQKNITDFTFPANRDEHPFTGIRVKQQH
HHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCEECCCCCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934931; 8041620; 9278503 [H]