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The map label for this gene is nreB [H]

Identifier: 52786523

GI number: 52786523

Start: 2677845

End: 2678927

Strand: Reverse

Name: nreB [H]

Synonym: BLi02788

Alternate gene names: 52786523

Gene position: 2678927-2677845 (Counterclockwise)

Preceding gene: 52786524

Following gene: 52786522

Centisome position: 63.44

GC content: 46.72

Gene sequence:

>1083_bases
ATGATGTGTCAAAACCCGGCTCGGTATACAGAAGAAGGAATGTCAAATGAAAGCGGCTATTTAAGGCAGATCTTCGACCA
TTTGCAGGATGGGATCATCATGATGGATCAGGAACGCACAATTCTGGTGATTAATCCGTCCGCAGAGAAGATGACGGGAT
GGAAACTAGGGGATAAGGTTCCGTACTGTTCCTACTGCCAAACGAGAAAACTGGAAGCGGGAGAAGAGCGTTGTTATTTG
CTGGCCAAGCGGGAAATTCCTTATTTTCTCTCCTCGATGCCCACCTATGAAGGGCGATTCGTCGACATGGAGATGAGTAC
GGCTGTCATTTCCGAAAACGGCGATCAAATGGAAGTTCTTTTGGTCCTGAAAGATTTAACAATGAAGAAAAAGGAGGAAG
AAGCGAAGATCTCCAAGCTGGTTCTGCAAAAAACGTTAGAGGCCCAGGAAAACGAACACAAGCGGCTTGCCCAGGAACTG
CACGATGGTGTAGGGCAATCGCTTTATTCGGTTTCAGTAGGCATTCAAGCGATTCAATCGCGCATGGAGCAGGAAGAAAC
ATTTAAAGATTACATGCAGGACATCATCGATGAACTGGAAAAAGCAATCCAAGACGTCAAGCTCTACTCTCTCCAGCTGC
GCCCGCACAGTCTCGATCAATTGGGGCTGATCCCTGCTGTCAAGCATCTTGTTTCCAGCTTAAACAAGACGCATCAGGAT
GTTTCTATTACGTTTGCCTCAAGCGATATCAGCAATCGCTTGCTGCCGATTTTTGAAATCAATTTGTACCGCATCATTCA
GGAGGCGCTTCATAATGCCCTGAAATATTCGCAGGCGACACTTATTGAAGTCATACTTTACAAAGAAGACGGCGAGCTCG
TTTTGATCATTCAGGACGATGGCATTGGTTTTGAACGCGGGCTGACAGAGGAAGGCCTGGGGTTCAAACATATGAAAGAG
AGAGTCGATCAGATGGATGGAAGTCTTCGAATTCACTCTGCACTTCAGAGTGGCACGACGATTAAAGTGAAAGTACAGGA
CAAGGAGGGGAAGGAGAGTGATCAGGGTATTGCTGGTCGATGA

Upstream 100 bases:

>100_bases
ATGTCTACGAGAAGCGCGTTTTATTCATTTTGAACAGCTTGAACAGAAGTTAAAAAGAGTCTATCAGTTGTACGAGGAAT
AGATAGCTAAGGGAGGGTCC

Downstream 100 bases:

>100_bases
CCATATGATGATTCGCAAGGGCATCCGTGTCCTTTTGGAAGGCTTTTCTGGAATTGAAATTGCGGGAGAAAGCAATAATG
GCAACGAAGCCGTTCTTAGC

Product: hypothetical protein

Products: NA

Alternate protein names: Nitrogen regulation protein B [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MMCQNPARYTEEGMSNESGYLRQIFDHLQDGIIMMDQERTILVINPSAEKMTGWKLGDKVPYCSYCQTRKLEAGEERCYL
LAKREIPYFLSSMPTYEGRFVDMEMSTAVISENGDQMEVLLVLKDLTMKKKEEEAKISKLVLQKTLEAQENEHKRLAQEL
HDGVGQSLYSVSVGIQAIQSRMEQEETFKDYMQDIIDELEKAIQDVKLYSLQLRPHSLDQLGLIPAVKHLVSSLNKTHQD
VSITFASSDISNRLLPIFEINLYRIIQEALHNALKYSQATLIEVILYKEDGELVLIIQDDGIGFERGLTEEGLGFKHMKE
RVDQMDGSLRIHSALQSGTTIKVKVQDKEGKESDQGIAGR

Sequences:

>Translated_360_residues
MMCQNPARYTEEGMSNESGYLRQIFDHLQDGIIMMDQERTILVINPSAEKMTGWKLGDKVPYCSYCQTRKLEAGEERCYL
LAKREIPYFLSSMPTYEGRFVDMEMSTAVISENGDQMEVLLVLKDLTMKKKEEEAKISKLVLQKTLEAQENEHKRLAQEL
HDGVGQSLYSVSVGIQAIQSRMEQEETFKDYMQDIIDELEKAIQDVKLYSLQLRPHSLDQLGLIPAVKHLVSSLNKTHQD
VSITFASSDISNRLLPIFEINLYRIIQEALHNALKYSQATLIEVILYKEDGELVLIIQDDGIGFERGLTEEGLGFKHMKE
RVDQMDGSLRIHSALQSGTTIKVKVQDKEGKESDQGIAGR
>Mature_360_residues
MMCQNPARYTEEGMSNESGYLRQIFDHLQDGIIMMDQERTILVINPSAEKMTGWKLGDKVPYCSYCQTRKLEAGEERCYL
LAKREIPYFLSSMPTYEGRFVDMEMSTAVISENGDQMEVLLVLKDLTMKKKEEEAKISKLVLQKTLEAQENEHKRLAQEL
HDGVGQSLYSVSVGIQAIQSRMEQEETFKDYMQDIIDELEKAIQDVKLYSLQLRPHSLDQLGLIPAVKHLVSSLNKTHQD
VSITFASSDISNRLLPIFEINLYRIIQEALHNALKYSQATLIEVILYKEDGELVLIIQDDGIGFERGLTEEGLGFKHMKE
RVDQMDGSLRIHSALQSGTTIKVKVQDKEGKESDQGIAGR

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxyg

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=233, Percent_Identity=27.0386266094421, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787474, Length=202, Percent_Identity=27.7227722772277, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788812, Length=208, Percent_Identity=26.9230769230769, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017203 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41017; Mature: 41017

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMCQNPARYTEEGMSNESGYLRQIFDHLQDGIIMMDQERTILVINPSAEKMTGWKLGDKV
CCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCEEEEECCCCCHHCCCCCCCCC
PYCSYCQTRKLEAGEERCYLLAKREIPYFLSSMPTYEGRFVDMEMSTAVISENGDQMEVL
CCCHHHHCCCCCCCCCCEEEEECCCCCHHHHCCCCCCCEEEEEEHHHHHCCCCCCCEEEE
LVLKDLTMKKKEEEAKISKLVLQKTLEAQENEHKRLAQELHDGVGQSLYSVSVGIQAIQS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMEQEETFKDYMQDIIDELEKAIQDVKLYSLQLRPHSLDQLGLIPAVKHLVSSLNKTHQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
VSITFASSDISNRLLPIFEINLYRIIQEALHNALKYSQATLIEVILYKEDGELVLIIQDD
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEECC
GIGFERGLTEEGLGFKHMKERVDQMDGSLRIHSALQSGTTIKVKVQDKEGKESDQGIAGR
CCCHHCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHCCCEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MMCQNPARYTEEGMSNESGYLRQIFDHLQDGIIMMDQERTILVINPSAEKMTGWKLGDKV
CCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCEEEEECCCCCHHCCCCCCCCC
PYCSYCQTRKLEAGEERCYLLAKREIPYFLSSMPTYEGRFVDMEMSTAVISENGDQMEVL
CCCHHHHCCCCCCCCCCEEEEECCCCCHHHHCCCCCCCEEEEEEHHHHHCCCCCCCEEEE
LVLKDLTMKKKEEEAKISKLVLQKTLEAQENEHKRLAQELHDGVGQSLYSVSVGIQAIQS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMEQEETFKDYMQDIIDELEKAIQDVKLYSLQLRPHSLDQLGLIPAVKHLVSSLNKTHQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
VSITFASSDISNRLLPIFEINLYRIIQEALHNALKYSQATLIEVILYKEDGELVLIIQDD
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEECC
GIGFERGLTEEGLGFKHMKERVDQMDGSLRIHSALQSGTTIKVKVQDKEGKESDQGIAGR
CCCHHCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHCCCEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12426351 [H]