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The map label for this gene is odhB [H]

Identifier: 52786003

GI number: 52786003

Start: 2216050

End: 2217330

Strand: Reverse

Name: odhB [H]

Synonym: BLi02259

Alternate gene names: 52786003

Gene position: 2217330-2216050 (Counterclockwise)

Preceding gene: 52786004

Following gene: 52786001

Centisome position: 52.51

GC content: 50.74

Gene sequence:

>1281_bases
ATGGCGGAAATTAAAGTACCTGAATTAGCGGAATCAATTTCAGAAGGAACGATAGCCCAATGGCTGAAACAACCGGGAGA
TTATGTAGAGCAAGGGGAATTCCTGCTTGAGCTGGAGACTGATAAAGTCAACGTGGAATTGACGGCAGAGCAGTCCGGCG
TTCTTCAGGAAGTGCTGAAAGATTCCGGTGACACGGTTCAGGTCGGCGAAATAATCGGAACGATTTCAGAAGGCGAAGGA
GAAGGAGGCAAATCAACGGCTCCGCAAGCTGACGCTCAAGAAAGCGCCGGCGCTTCGGAAGAGAAAGCAGCCTCGTCTGA
AAAGACAGCCGAACCTCGCGAAGGTGCGAGCGATGAGGCGGATACAGCCAAAACAAGAACTATCGCTTCTCCGGCTGCCC
GAAAGCTCGCCCGGGAAAAAGGCATCGATTTATCGGAAATCCCTACCGGTGATCCGCTCGGAAGGGTCAGAAAACAGGAT
GTTGAATCTTATCAAAAGAACGAGCAGCCGCCAAAAGCTCAGCCTGAACCAAAACGCGCAACACAGGCGCCGGCCGCTAA
ACAAACCGAAGATGCTGGTAAACCTGTAGAGCGTCAAAGAATGTCCCGCCGGAGACAGACGATCGCCAAACGCCTCGTTG
AAGTTCAGCACACCGCCGCTATGCTGACGACATTCAACGAAGTGGACATGACGGCGGTCATGAACTTAAGGAAGCGCCGA
AAAGATCAATTCCTGGAGCAGCATGACGTGAAGCTCGGATTCATGTCATTCTTTACAAAAGCTGTTGTCGCCGCGCTGAA
AAAATATCCGCTCCTCAACGCGGAAATTCAAGGCGACGAGCTTGTGATCAAAAAATTCTATGACATCGGAATCGCTGTTG
CTGCGCCGGATGGCCTCGTCGTACCGGTCGTGCGCGATGCGGACCGCAAAACCTTTGCCGATATTGAAAGGGATATCGGA
GAGCTTGCGAAGAAAGCGAGAAACAACAAATTGTCGCTCAACGAGCTTCAAGGCGGGTCCTTTACGATCACAAACGGAGG
AACGTTCGGTTCATTGTTATCGACTCCGATTTTGAACAGCCCGCAAGTTGGTATTTTGGGCATGCACAAAATCCAGCTGC
GTCCTGTAGCAATTGATGAAGAGCGCTTTGAAAACCGCCCGATGATGTACATTGCATTATCGTATGACCACCGGATCGTC
GACGGAAAAGAAGCGGTCGGCTTCCTGGTTACGATTAAAAATCTGCTTGAAGATCCTGAACAGCTTCTGTTGGAAGGTTA
A

Upstream 100 bases:

>100_bases
GTTCAAGTCCGGCAGAAGGGGATCCGACGGTTCATAAAAAGGAACAGGAACGCATTGTATCTGATAGCTTGACTCGCAAA
AATTAAGGGGGAACTATAAA

Downstream 100 bases:

>100_bases
TCATAAAAAAGCTTTGGCCGTAAGTACGGTCAAAGCTTTTTATTTATCATTTGCGGGGCAGGAGAATGATTGTCATTCTT
CTGTTTATTTGTTTTTTTGG

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MAEIKVPELAESISEGTIAQWLKQPGDYVEQGEFLLELETDKVNVELTAEQSGVLQEVLKDSGDTVQVGEIIGTISEGEG
EGGKSTAPQADAQESAGASEEKAASSEKTAEPREGASDEADTAKTRTIASPAARKLAREKGIDLSEIPTGDPLGRVRKQD
VESYQKNEQPPKAQPEPKRATQAPAAKQTEDAGKPVERQRMSRRRQTIAKRLVEVQHTAAMLTTFNEVDMTAVMNLRKRR
KDQFLEQHDVKLGFMSFFTKAVVAALKKYPLLNAEIQGDELVIKKFYDIGIAVAAPDGLVVPVVRDADRKTFADIERDIG
ELAKKARNNKLSLNELQGGSFTITNGGTFGSLLSTPILNSPQVGILGMHKIQLRPVAIDEERFENRPMMYIALSYDHRIV
DGKEAVGFLVTIKNLLEDPEQLLLEG

Sequences:

>Translated_426_residues
MAEIKVPELAESISEGTIAQWLKQPGDYVEQGEFLLELETDKVNVELTAEQSGVLQEVLKDSGDTVQVGEIIGTISEGEG
EGGKSTAPQADAQESAGASEEKAASSEKTAEPREGASDEADTAKTRTIASPAARKLAREKGIDLSEIPTGDPLGRVRKQD
VESYQKNEQPPKAQPEPKRATQAPAAKQTEDAGKPVERQRMSRRRQTIAKRLVEVQHTAAMLTTFNEVDMTAVMNLRKRR
KDQFLEQHDVKLGFMSFFTKAVVAALKKYPLLNAEIQGDELVIKKFYDIGIAVAAPDGLVVPVVRDADRKTFADIERDIG
ELAKKARNNKLSLNELQGGSFTITNGGTFGSLLSTPILNSPQVGILGMHKIQLRPVAIDEERFENRPMMYIALSYDHRIV
DGKEAVGFLVTIKNLLEDPEQLLLEG
>Mature_425_residues
AEIKVPELAESISEGTIAQWLKQPGDYVEQGEFLLELETDKVNVELTAEQSGVLQEVLKDSGDTVQVGEIIGTISEGEGE
GGKSTAPQADAQESAGASEEKAASSEKTAEPREGASDEADTAKTRTIASPAARKLAREKGIDLSEIPTGDPLGRVRKQDV
ESYQKNEQPPKAQPEPKRATQAPAAKQTEDAGKPVERQRMSRRRQTIAKRLVEVQHTAAMLTTFNEVDMTAVMNLRKRRK
DQFLEQHDVKLGFMSFFTKAVVAALKKYPLLNAEIQGDELVIKKFYDIGIAVAAPDGLVVPVVRDADRKTFADIERDIGE
LAKKARNNKLSLNELQGGSFTITNGGTFGSLLSTPILNSPQVGILGMHKIQLRPVAIDEERFENRPMMYIALSYDHRIVD
GKEAVGFLVTIKNLLEDPEQLLLEG

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=231, Percent_Identity=55.4112554112554, Blast_Score=264, Evalue=2e-70,
Organism=Homo sapiens, GI31711992, Length=439, Percent_Identity=29.3849658314351, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=30.820399113082, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI203098816, Length=451, Percent_Identity=30.820399113082, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI110671329, Length=438, Percent_Identity=27.3972602739726, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=36.5269461077844, Blast_Score=96, Evalue=9e-20,
Organism=Escherichia coli, GI1786946, Length=423, Percent_Identity=46.8085106382979, Blast_Score=357, Evalue=1e-99,
Organism=Escherichia coli, GI1786305, Length=444, Percent_Identity=31.3063063063063, Blast_Score=189, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI25146366, Length=419, Percent_Identity=39.8568019093079, Blast_Score=282, Evalue=3e-76,
Organism=Caenorhabditis elegans, GI17560088, Length=447, Percent_Identity=29.5302013422819, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=439, Percent_Identity=25.0569476082005, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17538894, Length=322, Percent_Identity=28.8819875776398, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320352, Length=426, Percent_Identity=40.3755868544601, Blast_Score=308, Evalue=9e-85,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=28.5077951002227, Blast_Score=169, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=51.1013215859031, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI18859875, Length=433, Percent_Identity=29.7921478060046, Blast_Score=174, Evalue=8e-44,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=31.5789473684211, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=31.5789473684211, Blast_Score=115, Evalue=6e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 46674; Mature: 46543

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIKVPELAESISEGTIAQWLKQPGDYVEQGEFLLELETDKVNVELTAEQSGVLQEVLK
CCCCCCHHHHHHHCCCHHHHHHHCCCCHHHCCCEEEEEECCEEEEEEECCHHHHHHHHHH
DSGDTVQVGEIIGTISEGEGEGGKSTAPQADAQESAGASEEKAASSEKTAEPREGASDEA
CCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCHHHHCCCCCCCCCCCCCCCCH
DTAKTRTIASPAARKLAREKGIDLSEIPTGDPLGRVRKQDVESYQKNEQPPKAQPEPKRA
HHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
TQAPAAKQTEDAGKPVERQRMSRRRQTIAKRLVEVQHTAAMLTTFNEVDMTAVMNLRKRR
HCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDQFLEQHDVKLGFMSFFTKAVVAALKKYPLLNAEIQGDELVIKKFYDIGIAVAAPDGLV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEECCCCEE
VPVVRDADRKTFADIERDIGELAKKARNNKLSLNELQGGSFTITNGGTFGSLLSTPILNS
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEECCCCHHHHHHCCCCCC
PQVGILGMHKIQLRPVAIDEERFENRPMMYIALSYDHRIVDGKEAVGFLVTIKNLLEDPE
CCCCEEEEEEEEEEEEEECHHHHCCCCEEEEEEECCCEEECCHHHHHHHHHHHHHHCCHH
QLLLEG
HHHCCC
>Mature Secondary Structure 
AEIKVPELAESISEGTIAQWLKQPGDYVEQGEFLLELETDKVNVELTAEQSGVLQEVLK
CCCCCHHHHHHHCCCHHHHHHHCCCCHHHCCCEEEEEECCEEEEEEECCHHHHHHHHHH
DSGDTVQVGEIIGTISEGEGEGGKSTAPQADAQESAGASEEKAASSEKTAEPREGASDEA
CCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCCHHHHCCCCCCCCCCCCCCCCH
DTAKTRTIASPAARKLAREKGIDLSEIPTGDPLGRVRKQDVESYQKNEQPPKAQPEPKRA
HHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
TQAPAAKQTEDAGKPVERQRMSRRRQTIAKRLVEVQHTAAMLTTFNEVDMTAVMNLRKRR
HCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDQFLEQHDVKLGFMSFFTKAVVAALKKYPLLNAEIQGDELVIKKFYDIGIAVAAPDGLV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEECCCCEE
VPVVRDADRKTFADIERDIGELAKKARNNKLSLNELQGGSFTITNGGTFGSLLSTPILNS
EEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEEECCCCHHHHHHCCCCCC
PQVGILGMHKIQLRPVAIDEERFENRPMMYIALSYDHRIVDGKEAVGFLVTIKNLLEDPE
CCCCEEEEEEEEEEEEEECHHHHCCCCEEEEEEECCCEEECCHHHHHHHHHHHHHHCCHH
QLLLEG
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2500417; 9384377 [H]