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The map label for this gene is glnA [H]

Identifier: 52785749

GI number: 52785749

Start: 1948131

End: 1949465

Strand: Direct

Name: glnA [H]

Synonym: BLi01993

Alternate gene names: 52785749

Gene position: 1948131-1949465 (Clockwise)

Preceding gene: 52785748

Following gene: 52785750

Centisome position: 46.14

GC content: 48.69

Gene sequence:

>1335_bases
ATGGCAAAGTATACAAGAGACGACATTGTTAAACTAGTAAATGAGGAGAATGTAAAATATATTCGCCTTCAGTTTACAGA
CATTCTTGGAACGATTAAAAACGTTGAAATTCCTGTGAGCCAGCTGGAAAAAGCACTTGATAATAAAGTCATGTTCGACG
GCTCTTCAATCGAGGGATTCGTCCGCATTGAAGAATCCGATATGTACTTATACCCTGACTTAGATACATTTGTGATTTTC
CCATGGACCGCCGAAAAAGGTAAAGTAGCCCGCTTCATCTGCGATATTTATAATCCGGACGGCACGCCGTTTGAAGGTGA
TCCGCGCAACAACTTAAAACGCATTTTGAAAGAAATGGAAGACATGGGCTTCTCAGATTTCAATCTTGGACCAGAGCCGG
AATTCTTCCTGTTCAAGCTAGACGAAAAAGGCGAGCCTACGCTTGAACTGAACGACAAAGGCGGATATTTCGACCTTGCG
CCGACTGATCTTGGCGAAAACTGCCGCCGCGACATCGTACTTGAGCTCGAAGAAATGGGCTTTGAAATCGAAGCGTCTCA
CCACGAAGTTGCACCTGGACAGCACGAAATCGACTTCAAATACGCCGGAGCCATCCGCGCTTGCGATGACATCCAAACGT
TCAAGCTCGTTGTTAAAACAATCGCGCGCAAACACGGCCTGCACGCAACATTCATGCCAAAACCGTTGTTCGGCGTAAAC
GGATCAGGCATGCACTGCAATCTATCACTCTTCCGAAACGGCGCCAACGCCTTCTTTGACAAAGACGCCGATCTTCAGCT
GAGTGAAACGGCAAAGCAATTCATCGCCGGCATCGTCAAGCACGCAACAAGCTTTACAGCTGTGACAAACCCGACTGTCA
ACTCTTACAAGCGCCTTGTTCCAGGCTATGAAGCACCTTGCTATGTGGCATGGAGCGCGCAAAACAGAAGCCCGTTAATC
CGCATCCCGGCTTCCCGCGGCATCAGCACGCGCGTAGAAGTCAGAAGCGTAGACCCTTCTGCGAACCCGTACCTTGCCTT
GAGCGTACTTCTTGCAGCAGGACTTGACGGCATCAAAAACAAGCTGGAAGCACCGGCTCCGATCGACCGCAACATCTATG
TCATGACAAAAGAAGAGCGCCTCGAAAACGGAATCGTCGACCTTCCTGCGACACTCGCAGACGCTCTCGAAGAATTCAAA
TCAAACGAAGTCATGGTCAAAGCCCTCGGCGACCATCTATTCGAACACTTCGTCGAAGCAAAAGAAATCGAATGGGATAT
GTTCCGCACACAAGTCCATCCTTGGGAGCGCGAGCAGTATATGTCTCAGTATTAA

Upstream 100 bases:

>100_bases
GGACGACATTCCGCCAGGGAGACATGTCCAGGTTTTTTCATTAATTTGTTAGCAACTGCATTGCTATATACATTTACCTT
TTAGAGGAGGAGTTTTACGA

Downstream 100 bases:

>100_bases
TCTAGGAATCCCTTGGCACCAGCGGTGTCAGGGGTTTTGAATTTTGGTCAAAAGAATGTAAGGGGTCTTCTTTTTGGTCT
TTGACCAAATTATGACCAAA

Product: hypothetical protein

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MAKYTRDDIVKLVNEENVKYIRLQFTDILGTIKNVEIPVSQLEKALDNKVMFDGSSIEGFVRIEESDMYLYPDLDTFVIF
PWTAEKGKVARFICDIYNPDGTPFEGDPRNNLKRILKEMEDMGFSDFNLGPEPEFFLFKLDEKGEPTLELNDKGGYFDLA
PTDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYAGAIRACDDIQTFKLVVKTIARKHGLHATFMPKPLFGVN
GSGMHCNLSLFRNGANAFFDKDADLQLSETAKQFIAGIVKHATSFTAVTNPTVNSYKRLVPGYEAPCYVAWSAQNRSPLI
RIPASRGISTRVEVRSVDPSANPYLALSVLLAAGLDGIKNKLEAPAPIDRNIYVMTKEERLENGIVDLPATLADALEEFK
SNEVMVKALGDHLFEHFVEAKEIEWDMFRTQVHPWEREQYMSQY

Sequences:

>Translated_444_residues
MAKYTRDDIVKLVNEENVKYIRLQFTDILGTIKNVEIPVSQLEKALDNKVMFDGSSIEGFVRIEESDMYLYPDLDTFVIF
PWTAEKGKVARFICDIYNPDGTPFEGDPRNNLKRILKEMEDMGFSDFNLGPEPEFFLFKLDEKGEPTLELNDKGGYFDLA
PTDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYAGAIRACDDIQTFKLVVKTIARKHGLHATFMPKPLFGVN
GSGMHCNLSLFRNGANAFFDKDADLQLSETAKQFIAGIVKHATSFTAVTNPTVNSYKRLVPGYEAPCYVAWSAQNRSPLI
RIPASRGISTRVEVRSVDPSANPYLALSVLLAAGLDGIKNKLEAPAPIDRNIYVMTKEERLENGIVDLPATLADALEEFK
SNEVMVKALGDHLFEHFVEAKEIEWDMFRTQVHPWEREQYMSQY
>Mature_443_residues
AKYTRDDIVKLVNEENVKYIRLQFTDILGTIKNVEIPVSQLEKALDNKVMFDGSSIEGFVRIEESDMYLYPDLDTFVIFP
WTAEKGKVARFICDIYNPDGTPFEGDPRNNLKRILKEMEDMGFSDFNLGPEPEFFLFKLDEKGEPTLELNDKGGYFDLAP
TDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYAGAIRACDDIQTFKLVVKTIARKHGLHATFMPKPLFGVNG
SGMHCNLSLFRNGANAFFDKDADLQLSETAKQFIAGIVKHATSFTAVTNPTVNSYKRLVPGYEAPCYVAWSAQNRSPLIR
IPASRGISTRVEVRSVDPSANPYLALSVLLAAGLDGIKNKLEAPAPIDRNIYVMTKEERLENGIVDLPATLADALEEFKS
NEVMVKALGDHLFEHFVEAKEIEWDMFRTQVHPWEREQYMSQY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=436, Percent_Identity=26.605504587156, Blast_Score=135, Evalue=7e-32,
Organism=Escherichia coli, GI1790301, Length=473, Percent_Identity=41.6490486257928, Blast_Score=321, Evalue=5e-89,
Organism=Escherichia coli, GI87081870, Length=447, Percent_Identity=29.082774049217, Blast_Score=166, Evalue=3e-42,
Organism=Drosophila melanogaster, GI281360453, Length=357, Percent_Identity=25.4901960784314, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI17136208, Length=357, Percent_Identity=25.4901960784314, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24580558, Length=357, Percent_Identity=25.4901960784314, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50271; Mature: 50139

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKYTRDDIVKLVNEENVKYIRLQFTDILGTIKNVEIPVSQLEKALDNKVMFDGSSIEGF
CCCCCHHHHHHHHCCCCCEEEEEEEHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCCEE
VRIEESDMYLYPDLDTFVIFPWTAEKGKVARFICDIYNPDGTPFEGDPRNNLKRILKEME
EEEECCCEEEECCCCEEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
DMGFSDFNLGPEPEFFLFKLDEKGEPTLELNDKGGYFDLAPTDLGENCRRDIVLELEEMG
HCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCEEECCCCCCCCHHHHHHEEEHHHCC
FEIEASHHEVAPGQHEIDFKYAGAIRACDDIQTFKLVVKTIARKHGLHATFMPKPLFGVN
EEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
GSGMHCNLSLFRNGANAFFDKDADLQLSETAKQFIAGIVKHATSFTAVTNPTVNSYKRLV
CCCCEEEEEEEECCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHC
PGYEAPCYVAWSAQNRSPLIRIPASRGISTRVEVRSVDPSANPYLALSVLLAAGLDGIKN
CCCCCCEEEEECCCCCCCEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCHHHHH
KLEAPAPIDRNIYVMTKEERLENGIVDLPATLADALEEFKSNEVMVKALGDHLFEHFVEA
HCCCCCCCCCCEEEEEHHHHHHCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHH
KEIEWDMFRTQVHPWEREQYMSQY
HHCHHHHHHHCCCCCHHHHHHHCC
>Mature Secondary Structure 
AKYTRDDIVKLVNEENVKYIRLQFTDILGTIKNVEIPVSQLEKALDNKVMFDGSSIEGF
CCCCHHHHHHHHCCCCCEEEEEEEHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCCEE
VRIEESDMYLYPDLDTFVIFPWTAEKGKVARFICDIYNPDGTPFEGDPRNNLKRILKEME
EEEECCCEEEECCCCEEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
DMGFSDFNLGPEPEFFLFKLDEKGEPTLELNDKGGYFDLAPTDLGENCRRDIVLELEEMG
HCCCCCCCCCCCCCEEEEEECCCCCCCEEECCCCCEEECCCCCCCCHHHHHHEEEHHHCC
FEIEASHHEVAPGQHEIDFKYAGAIRACDDIQTFKLVVKTIARKHGLHATFMPKPLFGVN
EEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCC
GSGMHCNLSLFRNGANAFFDKDADLQLSETAKQFIAGIVKHATSFTAVTNPTVNSYKRLV
CCCCEEEEEEEECCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHC
PGYEAPCYVAWSAQNRSPLIRIPASRGISTRVEVRSVDPSANPYLALSVLLAAGLDGIKN
CCCCCCEEEEECCCCCCCEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCHHHHH
KLEAPAPIDRNIYVMTKEERLENGIVDLPATLADALEEFKSNEVMVKALGDHLFEHFVEA
HCCCCCCCCCCEEEEEHHHHHHCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHH
KEIEWDMFRTQVHPWEREQYMSQY
HHCHHHHHHHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2906311; 2565853; 9384377; 11719184 [H]