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The map label for this gene is miaA

Identifier: 52785719

GI number: 52785719

Start: 1918719

End: 1919663

Strand: Direct

Name: miaA

Synonym: BLi01961

Alternate gene names: 52785719

Gene position: 1918719-1919663 (Clockwise)

Preceding gene: 52785718

Following gene: 52785720

Centisome position: 45.44

GC content: 48.15

Gene sequence:

>945_bases
GTGAATAAAACGAAGCCGCCGGTCATCGTTTTGATCGGACCGACCGCTGTCGGTAAAACAAAATTAAGCGTCATGCTGGC
CGAGCGTTTGAACGCGGAAATTATCAGCGGAGACTCAATGCAGATTTACAAAAGAATGGATATCGGCACAGCAAAAATCA
GAGAAGAGGAAATGAACGGGGTTCCGCATCATCTGATCGATATTAAAGAACCGACGGAATCTTTCTCGGTAGCTGAATAT
CAGGAGATCGTCAGACAAAAAATTGCAGAGATTGACAGGCGAGGGAAGCTTCCGATGATTGTCGGGGGAACCGGGCTCTA
TATCCAGTCTGTCCTTTACGACTATTCATTCACAGAGGAAGCAGGCGATCCTGAGTTCCGGGCGGAGATGGAGGCGTTCT
CTGCGAAGAGGGGGGCCGAATATGTTCACGATCTTTTGAAAGAACGCGATCCCGAAGCGGCAAGAGCCATTCATCCAAAT
AATAAACGAAGGGTCATCAGGGCGCTTGAAATCCTGCATACAACAGGAAAAACGATGTCCGAACATATGGAAGGACAGCG
GAAAGAGCTTTTATACACGACGGCTCTCATCGGTTTGACGATGGAGCGGGAAGTGCTCTATGACAGGATCAACAGCCGCG
TTGACCAGATGATGGATGAGGGGCTGCTGGATGAAGTGAAACTGCTTTATGATGAAAACGTAAGAAACTGCCAGTCTGTC
CAGGCGATCGGCTACAAAGAGCTTTATGCGCATCTCGAAGGCAGAGCGTCCTTGGAAGAGGCGGTTGAGACATTGAAGCG
GAATTCGAGACGCTACGCCAAAAGGCAGCTGACATGGTTTAGAAATCAAATGGATGTCGCATGGTTTGACATGACGCCCC
CTGTCAATATCGAGCAGAAAAAACAGGAAATTTTCACTTACATAGCAGGAAAGCTCGAACTTTAA

Upstream 100 bases:

>100_bases
GGAATGTTTGGAAAACAGTGAAAACGGGTAAAATGAAATGGGTGTTTTTGAATGTGAAATGGTATAATGGGAGGAGCTTG
TTTAAAGAGAGTGATCATCA

Downstream 100 bases:

>100_bases
TCGAAACAATATGGTATAGAGAAACAAGGAGGACCAGAAACATGAAATCGATAAATATTCAAGATCAGTTTTTGAATCAG
ATTCGGAAAGAAAATACATT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MNKTKPPVIVLIGPTAVGKTKLSVMLAERLNAEIISGDSMQIYKRMDIGTAKIREEEMNGVPHHLIDIKEPTESFSVAEY
QEIVRQKIAEIDRRGKLPMIVGGTGLYIQSVLYDYSFTEEAGDPEFRAEMEAFSAKRGAEYVHDLLKERDPEAARAIHPN
NKRRVIRALEILHTTGKTMSEHMEGQRKELLYTTALIGLTMEREVLYDRINSRVDQMMDEGLLDEVKLLYDENVRNCQSV
QAIGYKELYAHLEGRASLEEAVETLKRNSRRYAKRQLTWFRNQMDVAWFDMTPPVNIEQKKQEIFTYIAGKLEL

Sequences:

>Translated_314_residues
MNKTKPPVIVLIGPTAVGKTKLSVMLAERLNAEIISGDSMQIYKRMDIGTAKIREEEMNGVPHHLIDIKEPTESFSVAEY
QEIVRQKIAEIDRRGKLPMIVGGTGLYIQSVLYDYSFTEEAGDPEFRAEMEAFSAKRGAEYVHDLLKERDPEAARAIHPN
NKRRVIRALEILHTTGKTMSEHMEGQRKELLYTTALIGLTMEREVLYDRINSRVDQMMDEGLLDEVKLLYDENVRNCQSV
QAIGYKELYAHLEGRASLEEAVETLKRNSRRYAKRQLTWFRNQMDVAWFDMTPPVNIEQKKQEIFTYIAGKLEL
>Mature_314_residues
MNKTKPPVIVLIGPTAVGKTKLSVMLAERLNAEIISGDSMQIYKRMDIGTAKIREEEMNGVPHHLIDIKEPTESFSVAEY
QEIVRQKIAEIDRRGKLPMIVGGTGLYIQSVLYDYSFTEEAGDPEFRAEMEAFSAKRGAEYVHDLLKERDPEAARAIHPN
NKRRVIRALEILHTTGKTMSEHMEGQRKELLYTTALIGLTMEREVLYDRINSRVDQMMDEGLLDEVKLLYDENVRNCQSV
QAIGYKELYAHLEGRASLEEAVETLKRNSRRYAKRQLTWFRNQMDVAWFDMTPPVNIEQKKQEIFTYIAGKLEL

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=325, Percent_Identity=31.3846153846154, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1790613, Length=308, Percent_Identity=38.3116883116883, Blast_Score=213, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI25144712, Length=301, Percent_Identity=33.5548172757475, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324848, Length=290, Percent_Identity=34.8275862068965, Blast_Score=182, Evalue=8e-47,
Organism=Drosophila melanogaster, GI24649830, Length=340, Percent_Identity=33.5294117647059, Blast_Score=177, Evalue=7e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_BACLD (Q65JA9)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_079131.1
- RefSeq:   YP_091548.1
- ProteinModelPortal:   Q65JA9
- SMR:   Q65JA9
- STRING:   Q65JA9
- EnsemblBacteria:   EBBACT00000057847
- EnsemblBacteria:   EBBACT00000058537
- GeneID:   3031189
- GeneID:   3097789
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01961
- KEGG:   bli:BL05174
- NMPDR:   fig|279010.5.peg.2664
- eggNOG:   COG0324
- GeneTree:   EBGT00050000001497
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   BLIC279010-1:BLI01961-MONOMER
- BioCyc:   BLIC279010:BL05174-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 36156; Mature: 36156

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTKPPVIVLIGPTAVGKTKLSVMLAERLNAEIISGDSMQIYKRMDIGTAKIREEEMNG
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEECCCCHHHHHHHCCCHHHHHHHHHCC
VPHHLIDIKEPTESFSVAEYQEIVRQKIAEIDRRGKLPMIVGGTGLYIQSVLYDYSFTEE
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCCCC
AGDPEFRAEMEAFSAKRGAEYVHDLLKERDPEAARAIHPNNKRRVIRALEILHTTGKTMS
CCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHCCHHHH
EHMEGQRKELLYTTALIGLTMEREVLYDRINSRVDQMMDEGLLDEVKLLYDENVRNCQSV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHH
QAIGYKELYAHLEGRASLEEAVETLKRNSRRYAKRQLTWFRNQMDVAWFDMTPPVNIEQK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
KQEIFTYIAGKLEL
HHHHHHHHHHHCCC
>Mature Secondary Structure
MNKTKPPVIVLIGPTAVGKTKLSVMLAERLNAEIISGDSMQIYKRMDIGTAKIREEEMNG
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCEEECCCCHHHHHHHCCCHHHHHHHHHCC
VPHHLIDIKEPTESFSVAEYQEIVRQKIAEIDRRGKLPMIVGGTGLYIQSVLYDYSFTEE
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCCCC
AGDPEFRAEMEAFSAKRGAEYVHDLLKERDPEAARAIHPNNKRRVIRALEILHTTGKTMS
CCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHCCHHHH
EHMEGQRKELLYTTALIGLTMEREVLYDRINSRVDQMMDEGLLDEVKLLYDENVRNCQSV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHH
QAIGYKELYAHLEGRASLEEAVETLKRNSRRYAKRQLTWFRNQMDVAWFDMTPPVNIEQK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHH
KQEIFTYIAGKLEL
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA