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The map label for this gene is truB

Identifier: 52785650

GI number: 52785650

Start: 1849695

End: 1850624

Strand: Direct

Name: truB

Synonym: BLi01891

Alternate gene names: 52785650

Gene position: 1849695-1850624 (Clockwise)

Preceding gene: 52785649

Following gene: 52785651

Centisome position: 43.8

GC content: 51.94

Gene sequence:

>930_bases
ATGTATAACGGAGTCCTTCTATTACATAAACCGGTCGGCATGACCTCGCACGACTGCGTCATGAAGATCCGCAAACTGCT
GAAAACAAAAAAGGTCGGCCATACCGGTACACTGGATCCCGAGGTGTCGGGCGTTCTCCCGATCTGCGTCGGGAGGGCAA
CCAAAATCGTCGAGTATTTGACCGAAAAGTCCAAAACATATGATGCAGAGGTCACGCTTGGCTGTTCCACGGAGACAGAG
GATCAGACGGGGGAAGTCACAGAAAAGAAACCCGTCTTAGCGCCGCCTGATGAACAGACCGTGCAGAGCGTCCTGAGATC
TCTTGAAGGAACGATTGAGCAGGTTCCGCCGATGTATTCAGCTGTCAAGGTAGGCGGCAAAAAGCTGTATGAATACGCGC
GGGCTGGAATCGAGGTTGAACGGCCGAAGCGGACCATCACCATCCATCATATCGAACTGACATCCGAGATCCGCCATGAG
GGGGATAAAGCCCGCTTCCGGTTTGTTGTGACATGTTCAAAAGGCACATATGTCAGGACCTTGGCGGTAACCATCGGGGA
GAAGCTCGGATATCCCGCGCATATGTCCAATCTGGTCAGGACCGCGTCAGGCCCTTTCACCCTTGACGAATGCTTGACCT
TTGAAGACGTCGAAGGCTTGATCGCTGACGGAACATTGTCTGAAAAGCTCGTCCCCATCGAACGTGCGCTTGATCATTTG
CCGAAATGGATAATTAGTGATACATTAGCTAAGAAAGTGGAAAATGGAGCGGTGCTTGAAACGCCCGGCAGCTTTTCTCA
TTTGACGAGCGAAGACCGCATTGCGGTCTTTACGGAAGCAGGCCGGTGCACAGCCGTCTATTATCCGCATCCGACCAAAA
CCGGTTTGTTGAAGCCGGCAAAAGTACTCGTGCAAAAAAGCGAACAATAG

Upstream 100 bases:

>100_bases
AGCTGAACGCAAACAAAGAAGAATCATAAGCTGAAAAAGGATAGGCGGGCGACTGCCTGTCCTTTTTAGCTCCAAATGAG
AAAGGGGCGAAGAAGCACGA

Downstream 100 bases:

>100_bases
AAAAGGTGACCGTTCTTGAAGACGATACATATTTCCCACCCACACGACTTGAAGCGTGAGGATTTTCCGACATCAGTTAT
GGCATTAGGTTATTTTGACG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MYNGVLLLHKPVGMTSHDCVMKIRKLLKTKKVGHTGTLDPEVSGVLPICVGRATKIVEYLTEKSKTYDAEVTLGCSTETE
DQTGEVTEKKPVLAPPDEQTVQSVLRSLEGTIEQVPPMYSAVKVGGKKLYEYARAGIEVERPKRTITIHHIELTSEIRHE
GDKARFRFVVTCSKGTYVRTLAVTIGEKLGYPAHMSNLVRTASGPFTLDECLTFEDVEGLIADGTLSEKLVPIERALDHL
PKWIISDTLAKKVENGAVLETPGSFSHLTSEDRIAVFTEAGRCTAVYYPHPTKTGLLKPAKVLVQKSEQ

Sequences:

>Translated_309_residues
MYNGVLLLHKPVGMTSHDCVMKIRKLLKTKKVGHTGTLDPEVSGVLPICVGRATKIVEYLTEKSKTYDAEVTLGCSTETE
DQTGEVTEKKPVLAPPDEQTVQSVLRSLEGTIEQVPPMYSAVKVGGKKLYEYARAGIEVERPKRTITIHHIELTSEIRHE
GDKARFRFVVTCSKGTYVRTLAVTIGEKLGYPAHMSNLVRTASGPFTLDECLTFEDVEGLIADGTLSEKLVPIERALDHL
PKWIISDTLAKKVENGAVLETPGSFSHLTSEDRIAVFTEAGRCTAVYYPHPTKTGLLKPAKVLVQKSEQ
>Mature_309_residues
MYNGVLLLHKPVGMTSHDCVMKIRKLLKTKKVGHTGTLDPEVSGVLPICVGRATKIVEYLTEKSKTYDAEVTLGCSTETE
DQTGEVTEKKPVLAPPDEQTVQSVLRSLEGTIEQVPPMYSAVKVGGKKLYEYARAGIEVERPKRTITIHHIELTSEIRHE
GDKARFRFVVTCSKGTYVRTLAVTIGEKLGYPAHMSNLVRTASGPFTLDECLTFEDVEGLIADGTLSEKLVPIERALDHL
PKWIISDTLAKKVENGAVLETPGSFSHLTSEDRIAVFTEAGRCTAVYYPHPTKTGLLKPAKVLVQKSEQ

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=231, Percent_Identity=36.7965367965368, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI4503337, Length=284, Percent_Identity=28.8732394366197, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI215599015, Length=284, Percent_Identity=28.8732394366197, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI2367200, Length=271, Percent_Identity=39.1143911439114, Blast_Score=188, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17553978, Length=285, Percent_Identity=27.3684210526316, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323204, Length=295, Percent_Identity=30.5084745762712, Blast_Score=109, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6324037, Length=196, Percent_Identity=33.1632653061224, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281364189, Length=294, Percent_Identity=30.2721088435374, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281364187, Length=294, Percent_Identity=30.2721088435374, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281364185, Length=294, Percent_Identity=30.2721088435374, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281364183, Length=294, Percent_Identity=30.2721088435374, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI62471759, Length=294, Percent_Identity=30.2721088435374, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17975520, Length=294, Percent_Identity=30.2721088435374, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_BACLD (Q65JH8)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_079064.2
- RefSeq:   YP_091479.1
- ProteinModelPortal:   Q65JH8
- SMR:   Q65JH8
- STRING:   Q65JH8
- EnsemblBacteria:   EBBACT00000056621
- EnsemblBacteria:   EBBACT00000061392
- GeneID:   3031088
- GeneID:   3097742
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01891
- KEGG:   bli:BL01222
- NMPDR:   fig|279010.5.peg.2595
- eggNOG:   COG0130
- GeneTree:   EBGT00050000002629
- HOGENOM:   HBG397258
- ProtClustDB:   PRK01550
- BioCyc:   BLIC279010-1:BLI01891-MONOMER
- BioCyc:   BLIC279010:BL01222-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 34017; Mature: 34017

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: ACT_SITE 39-39

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNGVLLLHKPVGMTSHDCVMKIRKLLKTKKVGHTGTLDPEVSGVLPICVGRATKIVEYL
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TEKSKTYDAEVTLGCSTETEDQTGEVTEKKPVLAPPDEQTVQSVLRSLEGTIEQVPPMYS
HHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHH
AVKVGGKKLYEYARAGIEVERPKRTITIHHIELTSEIRHEGDKARFRFVVTCSKGTYVRT
HHHHHHHHHHHHHHCCCEEECCCEEEEEEEEEEHHHHHCCCCCEEEEEEEEECCCCEEEH
LAVTIGEKLGYPAHMSNLVRTASGPFTLDECLTFEDVEGLIADGTLSEKLVPIERALDHL
HHHHHHHHCCCCHHHHHHHHHCCCCEEHHHHCCHHHHCCEEECCCCCHHCCHHHHHHHHH
PKWIISDTLAKKVENGAVLETPGSFSHLTSEDRIAVFTEAGRCTAVYYPHPTKTGLLKPA
HHHHHHHHHHHHHCCCCEEECCCCCHHCCCCCCEEEEEECCCEEEEECCCCCCCCCCCHH
KVLVQKSEQ
HHHHHCCCC
>Mature Secondary Structure
MYNGVLLLHKPVGMTSHDCVMKIRKLLKTKKVGHTGTLDPEVSGVLPICVGRATKIVEYL
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TEKSKTYDAEVTLGCSTETEDQTGEVTEKKPVLAPPDEQTVQSVLRSLEGTIEQVPPMYS
HHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHH
AVKVGGKKLYEYARAGIEVERPKRTITIHHIELTSEIRHEGDKARFRFVVTCSKGTYVRT
HHHHHHHHHHHHHHCCCEEECCCEEEEEEEEEEHHHHHCCCCCEEEEEEEEECCCCEEEH
LAVTIGEKLGYPAHMSNLVRTASGPFTLDECLTFEDVEGLIADGTLSEKLVPIERALDHL
HHHHHHHHCCCCHHHHHHHHHCCCCEEHHHHCCHHHHCCEEECCCCCHHCCHHHHHHHHH
PKWIISDTLAKKVENGAVLETPGSFSHLTSEDRIAVFTEAGRCTAVYYPHPTKTGLLKPA
HHHHHHHHHHHHHCCCCEEECCCCCHHCCCCCCEEEEEECCCEEEEECCCCCCCCCCCHH
KVLVQKSEQ
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA