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The map label for this gene is nusA [H]

Identifier: 52785644

GI number: 52785644

Start: 1845095

End: 1846213

Strand: Direct

Name: nusA [H]

Synonym: BLi01885

Alternate gene names: 52785644

Gene position: 1845095-1846213 (Clockwise)

Preceding gene: 52785643

Following gene: 52785645

Centisome position: 43.7

GC content: 49.42

Gene sequence:

>1119_bases
ATGAGTAGTGAGTTGTTAGATGCCCTGACTGTTCTTGAAAAAGAAAAGGGAATCAGTAAGGACATTATTATTGAAGCAAT
TGAAGCTGCGCTCATTTCCGCTTATAAGCGGAACTTCAACCAGGCGCAGAACGTCAGGGTAGATTTAAACCGCGACACCG
GTTCAATCCGCGTTTTCGCCCGCAAAGATGTCGTGGATGAAGTGTATGATCCGCGCCTTGAAATCTCGATTGAAGATGCC
GTAAACATCAATCCGAACTACATTGTCGGAGATGTCGTGGAAATCGAAGTGACGCCGAAAGATTTCGGCCGCATCGCTGC
CCAGACGGCAAAGCAGGTCGTGACCCAGCGCGTCCGCGAAGCGGAGCGCGGCGTCATCTATTCTGAATTCATCGACCGCG
AGGAAGATATCATGACAGGCATCGTCCAGCGGATCGACAATAAGTTCATCTATGTCTCTTTAGGAAAAATCGAGGCGCTT
CTCCCGGTTGCAGAACAGATGCCGAATGAAGTCTACAAGCCTCATGACCGGATCAAGGTGTTCATTACAAAAGTCGAAAA
AACGACGAAGGGTCCGCAGATTTACGTTTCAAGAACTCATCCAGGTTTATTAAAGCGTCTCTTTGAAATCGAAGTGCCGG
AAATTTACGATGGAACGGTCGAGCTCAAATCCGTGGCGAGAGAAGCGGGAGACCGCTCGAAGATTTCCGTCCGCACGGAT
AATCCCGACATCGATCCCGTCGGCGCCTGCGTCGGACCGAAGGGTCAGCGCGTTCAGGCGATCGTCAATGAATTGAAAGG
CGAGAAAATTGATATTGTTCACTGGTCAAACGACCCGGTTGAATTTGTCGCCAATGCGCTGAGCCCTTCAAAAGTGCTCG
ACGTCATCGTCAATGAAGAGGATAAAGCGACAACGGTTGTCGTTCCTGACTATCAGCTGTCGCTCGCCATCGGAAAAAGA
GGTCAAAACGCCCGCCTGGCAGCCAAGCTGACAAGCTGGAAGATCGATATTAAAAGCGAAACAGATGCAAGAGAGCTTGG
AATTTATCCAAGGGAGCTTGAAGAAGAGGAGCCGGAAGAGCTTTTCTTGGAAAGCGAAACTCCAGAATCGGATGAATAA

Upstream 100 bases:

>100_bases
CGCCGGAAAACGATCAATATTCCATATGACAAAGTAGCCAAAGCGAGGCTGGCTGTTTCGTTCAATTAATTTTTGACTGT
TTAAGGGGGAAACCATAAAC

Downstream 100 bases:

>100_bases
GAGGTGACCTTTGGTGAAAAGCCGAAAGAAGATCCCGCTTAGAAAATGCGTCGTAACAGGGGAAATGAAGCCTAAAAAAG
AGCTCATCCGCGTCGTCCGT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSSELLDALTVLEKEKGISKDIIIEAIEAALISAYKRNFNQAQNVRVDLNRDTGSIRVFARKDVVDEVYDPRLEISIEDA
VNINPNYIVGDVVEIEVTPKDFGRIAAQTAKQVVTQRVREAERGVIYSEFIDREEDIMTGIVQRIDNKFIYVSLGKIEAL
LPVAEQMPNEVYKPHDRIKVFITKVEKTTKGPQIYVSRTHPGLLKRLFEIEVPEIYDGTVELKSVAREAGDRSKISVRTD
NPDIDPVGACVGPKGQRVQAIVNELKGEKIDIVHWSNDPVEFVANALSPSKVLDVIVNEEDKATTVVVPDYQLSLAIGKR
GQNARLAAKLTSWKIDIKSETDARELGIYPRELEEEEPEELFLESETPESDE

Sequences:

>Translated_372_residues
MSSELLDALTVLEKEKGISKDIIIEAIEAALISAYKRNFNQAQNVRVDLNRDTGSIRVFARKDVVDEVYDPRLEISIEDA
VNINPNYIVGDVVEIEVTPKDFGRIAAQTAKQVVTQRVREAERGVIYSEFIDREEDIMTGIVQRIDNKFIYVSLGKIEAL
LPVAEQMPNEVYKPHDRIKVFITKVEKTTKGPQIYVSRTHPGLLKRLFEIEVPEIYDGTVELKSVAREAGDRSKISVRTD
NPDIDPVGACVGPKGQRVQAIVNELKGEKIDIVHWSNDPVEFVANALSPSKVLDVIVNEEDKATTVVVPDYQLSLAIGKR
GQNARLAAKLTSWKIDIKSETDARELGIYPRELEEEEPEELFLESETPESDE
>Mature_371_residues
SSELLDALTVLEKEKGISKDIIIEAIEAALISAYKRNFNQAQNVRVDLNRDTGSIRVFARKDVVDEVYDPRLEISIEDAV
NINPNYIVGDVVEIEVTPKDFGRIAAQTAKQVVTQRVREAERGVIYSEFIDREEDIMTGIVQRIDNKFIYVSLGKIEALL
PVAEQMPNEVYKPHDRIKVFITKVEKTTKGPQIYVSRTHPGLLKRLFEIEVPEIYDGTVELKSVAREAGDRSKISVRTDN
PDIDPVGACVGPKGQRVQAIVNELKGEKIDIVHWSNDPVEFVANALSPSKVLDVIVNEEDKATTVVVPDYQLSLAIGKRG
QNARLAAKLTSWKIDIKSETDARELGIYPRELEEEEPEELFLESETPESDE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=343, Percent_Identity=40.2332361516035, Blast_Score=273, Evalue=9e-75,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 41798; Mature: 41667

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSELLDALTVLEKEKGISKDIIIEAIEAALISAYKRNFNQAQNVRVDLNRDTGSIRVFA
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEE
RKDVVDEVYDPRLEISIEDAVNINPNYIVGDVVEIEVTPKDFGRIAAQTAKQVVTQRVRE
EHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHH
AERGVIYSEFIDREEDIMTGIVQRIDNKFIYVSLGKIEALLPVAEQMPNEVYKPHDRIKV
HHCCEEHHHHHCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCHHHCCCCCCEEE
FITKVEKTTKGPQIYVSRTHPGLLKRLFEIEVPEIYDGTVELKSVAREAGDRSKISVRTD
EEEEHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECC
NPDIDPVGACVGPKGQRVQAIVNELKGEKIDIVHWSNDPVEFVANALSPSKVLDVIVNEE
CCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCCHHHHHEEEECCC
DKATTVVVPDYQLSLAIGKRGQNARLAAKLTSWKIDIKSETDARELGIYPRELEEEEPEE
CCEEEEEECCCEEEEEECCCCCCCEEEEEEEEEEEEECCCCCHHHHCCCCHHCCCCCCHH
LFLESETPESDE
HEEECCCCCCCC
>Mature Secondary Structure 
SSELLDALTVLEKEKGISKDIIIEAIEAALISAYKRNFNQAQNVRVDLNRDTGSIRVFA
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEE
RKDVVDEVYDPRLEISIEDAVNINPNYIVGDVVEIEVTPKDFGRIAAQTAKQVVTQRVRE
EHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHH
AERGVIYSEFIDREEDIMTGIVQRIDNKFIYVSLGKIEALLPVAEQMPNEVYKPHDRIKV
HHCCEEHHHHHCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCHHHCCCCCCEEE
FITKVEKTTKGPQIYVSRTHPGLLKRLFEIEVPEIYDGTVELKSVAREAGDRSKISVRTD
EEEEHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEECC
NPDIDPVGACVGPKGQRVQAIVNELKGEKIDIVHWSNDPVEFVANALSPSKVLDVIVNEE
CCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCHHHHHHHCCCHHHHHEEEECCC
DKATTVVVPDYQLSLAIGKRGQNARLAAKLTSWKIDIKSETDARELGIYPRELEEEEPEE
CCEEEEEECCCEEEEEECCCCCCCEEEEEEEEEEEEECCCCCHHHHCCCCHHCCCCCCHH
LFLESETPESDE
HEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8491709; 9384377 [H]