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The map label for this gene is pyrP [H]

Identifier: 52785527

GI number: 52785527

Start: 1724469

End: 1725773

Strand: Direct

Name: pyrP [H]

Synonym: BLi01768

Alternate gene names: 52785527

Gene position: 1724469-1725773 (Clockwise)

Preceding gene: 52785526

Following gene: 52785528

Centisome position: 40.84

GC content: 52.41

Gene sequence:

>1305_bases
ATGAGTCAGAAAAAAGTGAACCTTGGTGTCAGAGAAGTACCAAAACCTTTGAATTGGATATCTTTCAGCCTGCAGCATCT
GTTTGCCATGTTCGGAGCGACGATCCTTGTGCCGAAGCTTGTAGGCTTAAGCCCGGGCGTGGCCCTCATTACAAGCGGGC
TTGGAACGCTTGCGTATCTCTTGATTACAAAAGGACAGATCCCGGCTTACTTAGGTTCATCCTTTGCATTTATTGCCCCG
ATTATCGCGGCAAAAGCCGCCGGAGGCCCTGGCGCGGCCATGATCGGCGCATTTATGGCAGGCATCGCCTACGGGCTGAT
CGCCCTTCTCATCCAGCAGCTTGGAACGGACTGGCTGATGAGGCTTCTGCCGCCTGTTGTCGTCGGTCCGGTCATTATCG
TCATCGGACTCGGGCTGGCAAGCACGGCTGTCAATATGGCCATGTATGAAGATTCGGGAGCAAAAGTGCTCGTCTACAGC
GCCAAGCATTTTGGCGTCGCAGGATTCACACTCGCGCTCACCATAATCTGCGCGATTTTCTTGAGAGGATTTTTCAGCCT
GATTCCTGTCTTGATCGGAATTATCGGCGGATACTTGTTCGCCCTGACCCAGGGAATCGTCGACTTCCAGATGGTTCTTG
ACGCCAAATGGTTTGCTGTTCCGGACTTCGTGCTTCCGTTTGTCGACTATACGCCGAGCGCGGCGGCTGTCGGAATCGGA
ATCGCCATGGTGCCCGTCGCTTTCGTGACAATGTCAGAACATATCGGACACCAAATGGTATTAAGCAAGGTCGTCGGCCA
GGATTTTATTAAGAAGCCCGGGCTTCACCGCTCGATTTTCGGCGACAGCGTCGCAACGATGCTTGCATCATGCATCGGCG
GACCCCCGACAACGACTTACGGAGAAAACATCGGCGTACTCGCCATCACAAGAGTATTCAGCGTATTTGTCATCGGCGGA
GCGGCTGTCATTGCACTGTGCTTCGGATTTATCGGCAAAATTTCGGCCCTGATCAGCTCTGTCCCTTCCGCAGTCATGGG
AGGCGTTTCCTTCCTGCTGTTCGGTATCATCGCATCCAGCGGACTGCGCATGCTGATCGATAATAAAATTGACTTGGAAA
ACAAGCGAAACCTGATCATCTCATCCGTCATTCTCGTCATCGGAGTGGGCGGCGCATTTATCAAGATCTCAGGGTTTGAG
CTGTCAAGCATGGCGCTTGCGGCGATCGTCGGAGTGGTTTTAAACCTTGTCCTGCCTGAATCAAAGGGAGATCAGGACGG
AATTGAAATAGAAGAGCATATTTAA

Upstream 100 bases:

>100_bases
CTTTGCGCAATTTAAGCAAAGAGGCTTTTTTAATGGGCATAATTCATTTCAGGATATGAAGACATCATAGACAGTGAGTC
GATCATCAGGAGGAAAAAAT

Downstream 100 bases:

>100_bases
AAACCTTTTAAATGAAGTCCAGAGAGGCTTGAAAGGGTTGCGGAGAGAGAAGTGCAAAAAAGCGCATTCTCTGATTCACT
GTACCCCAAGCCGTTATGGC

Product: PyrP

Products: Proton [Cytoplasm]; uracil [Cytoplasm] [C]

Alternate protein names: Uracil transporter [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MSQKKVNLGVREVPKPLNWISFSLQHLFAMFGATILVPKLVGLSPGVALITSGLGTLAYLLITKGQIPAYLGSSFAFIAP
IIAAKAAGGPGAAMIGAFMAGIAYGLIALLIQQLGTDWLMRLLPPVVVGPVIIVIGLGLASTAVNMAMYEDSGAKVLVYS
AKHFGVAGFTLALTIICAIFLRGFFSLIPVLIGIIGGYLFALTQGIVDFQMVLDAKWFAVPDFVLPFVDYTPSAAAVGIG
IAMVPVAFVTMSEHIGHQMVLSKVVGQDFIKKPGLHRSIFGDSVATMLASCIGGPPTTTYGENIGVLAITRVFSVFVIGG
AAVIALCFGFIGKISALISSVPSAVMGGVSFLLFGIIASSGLRMLIDNKIDLENKRNLIISSVILVIGVGGAFIKISGFE
LSSMALAAIVGVVLNLVLPESKGDQDGIEIEEHI

Sequences:

>Translated_434_residues
MSQKKVNLGVREVPKPLNWISFSLQHLFAMFGATILVPKLVGLSPGVALITSGLGTLAYLLITKGQIPAYLGSSFAFIAP
IIAAKAAGGPGAAMIGAFMAGIAYGLIALLIQQLGTDWLMRLLPPVVVGPVIIVIGLGLASTAVNMAMYEDSGAKVLVYS
AKHFGVAGFTLALTIICAIFLRGFFSLIPVLIGIIGGYLFALTQGIVDFQMVLDAKWFAVPDFVLPFVDYTPSAAAVGIG
IAMVPVAFVTMSEHIGHQMVLSKVVGQDFIKKPGLHRSIFGDSVATMLASCIGGPPTTTYGENIGVLAITRVFSVFVIGG
AAVIALCFGFIGKISALISSVPSAVMGGVSFLLFGIIASSGLRMLIDNKIDLENKRNLIISSVILVIGVGGAFIKISGFE
LSSMALAAIVGVVLNLVLPESKGDQDGIEIEEHI
>Mature_433_residues
SQKKVNLGVREVPKPLNWISFSLQHLFAMFGATILVPKLVGLSPGVALITSGLGTLAYLLITKGQIPAYLGSSFAFIAPI
IAAKAAGGPGAAMIGAFMAGIAYGLIALLIQQLGTDWLMRLLPPVVVGPVIIVIGLGLASTAVNMAMYEDSGAKVLVYSA
KHFGVAGFTLALTIICAIFLRGFFSLIPVLIGIIGGYLFALTQGIVDFQMVLDAKWFAVPDFVLPFVDYTPSAAAVGIGI
AMVPVAFVTMSEHIGHQMVLSKVVGQDFIKKPGLHRSIFGDSVATMLASCIGGPPTTTYGENIGVLAITRVFSVFVIGGA
AVIALCFGFIGKISALISSVPSAVMGGVSFLLFGIIASSGLRMLIDNKIDLENKRNLIISSVILVIGVGGAFIKISGFEL
SSMALAAIVGVVLNLVLPESKGDQDGIEIEEHI

Specific function: Transport of uracil in the cell [H]

COG id: COG2233

COG function: function code F; Xanthine/uracil permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily [H]

Homologues:

Organism=Homo sapiens, GI44680148, Length=446, Percent_Identity=26.2331838565022, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI40316845, Length=446, Percent_Identity=26.2331838565022, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI44680145, Length=298, Percent_Identity=25.8389261744966, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI44680143, Length=298, Percent_Identity=25.8389261744966, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788843, Length=417, Percent_Identity=44.6043165467626, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI87081818, Length=430, Percent_Identity=36.7441860465116, Blast_Score=248, Evalue=8e-67,
Organism=Escherichia coli, GI87082181, Length=404, Percent_Identity=28.2178217821782, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1790087, Length=448, Percent_Identity=23.8839285714286, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17541904, Length=229, Percent_Identity=27.9475982532751, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21356175, Length=430, Percent_Identity=24.8837209302326, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006042
- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 45212; Mature: 45081

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS01116 XANTH_URACIL_PERMASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCHHCCC
>Mature Secondary Structure 
SQKKVNLGVREVPKPLNWISFSLQHLFAMFGATILVPKLVGLSPGVALITSGLGTLAYL
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LITKGQIPAYLGSSFAFIAPIIAAKAAGGPGAAMIGAFMAGIAYGLIALLIQQLGTDWLM
HHCCCCCCHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
RLLPPVVVGPVIIVIGLGLASTAVNMAMYEDSGAKVLVYSAKHFGVAGFTLALTIICAIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHH
LRGFFSLIPVLIGIIGGYLFALTQGIVDFQMVLDAKWFAVPDFVLPFVDYTPSAAAVGIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHCCCCCHHHHHHH
IAMVPVAFVTMSEHIGHQMVLSKVVGQDFIKKPGLHRSIFGDSVATMLASCIGGPPTTTY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
GENIGVLAITRVFSVFVIGGAAVIALCFGFIGKISALISSVPSAVMGGVSFLLFGIIASS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLRMLIDNKIDLENKRNLIISSVILVIGVGGAFIKISGFELSSMALAAIVGVVLNLVLPE
CCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCC
SKGDQDGIEIEEHI
CCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; uracil [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uracil [Periplasm] = Proton [Cytoplasm] + uracil [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1709162; 8206849; 9384377 [H]