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The map label for this gene is pyrB

Identifier: 52785528

GI number: 52785528

Start: 1725917

End: 1726837

Strand: Direct

Name: pyrB

Synonym: BLi01769

Alternate gene names: 52785528

Gene position: 1725917-1726837 (Clockwise)

Preceding gene: 52785527

Following gene: 52785529

Centisome position: 40.87

GC content: 48.97

Gene sequence:

>921_bases
ATGAAACACTTGACCGCAATGAATGAGCTTAGTCTTGAGGATATTCACGAGCTGATTGAAGAGGCGAGAGAGCTGAAAAA
AGGCAAGTCCGATTCCGTTCTCTCCGGAAAGTTCGCTGCCAACCTGTTTTTTGAACCGAGCACAAGAACGCGCTTCAGCT
TCGAAGTGGCTGAAAAGAAACTCGGCATGAATGTGTTGAACCTGGACGGTGTTTCAACGAGCGTACAAAAAGGAGAATCG
TTGTACGATACCGTCAAGACCCTCGAATCCATCGGCGCTGATGTATGTGTGATCAGACACTCTCATGATCATTATTACGA
TGAACTCATCGGACATGTCGGCATTCCGGTGATCAATGCCGGAGACGGCTGCGGTCAGCATCCCACTCAGTCTTTGCTCG
ATTTGATGACGATTCACGAGGAATGGGGCAGATTTGCCGGACTGACGGTATCCATACACGGAGATATCAAGCACAGCAGA
GTGGCAAGGTCCAATGCCGAAGTGCTGACAAGACTCGGAGCAAAGGTTCTTTTCTCAGGTCCAGACGAATGGCGGGATGA
AGACAATCCATACGGGACATATGTGTCGCCTGACGAAGCGGTTGCACATTCGGATGTCGTGATGCTTCTCCGGATTCAGC
ACGAACGGCATGAGAAAAAGGCGGCAGAACGCGATTATCTCGAAACATTCGGATTGTCGCTCAAGCGGGCGGAGCTCTTG
AAAAAAGACGCGGTCATCATGCACCCCGCACCTGTCAACCGAGGCGTCGAGATTGACAGTGCACTGGTCGAATCCGGCCG
GTCGAGGATTTTTAAACAAATGGAAAACGGAGTATATATCAGAATGGCGGTTTTAAAAAGAGCGCTTCTAAACGGCGAAA
ACAAAAAAAGGGGAGATCAGGCTTATGTCTTATTTAATTAA

Upstream 100 bases:

>100_bases
AGAGAGAAGTGCAAAAAAGCGCATTCTCTGATTCACTGTACCCCAAGCCGTTATGGCCTGGGGTTTTTTTACGGGAAATT
AAGCCTGAGGGGGAGAAAAA

Downstream 100 bases:

>100_bases
AAACGGCTTCATGCTCGACGAAAAAGGCGAAAAAGTACAGCGCGATATCAGGGTTGAAGGCGATGCAATCAGCGAGATCG
GAAGCCTTGAGGCCGCTTCG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKHLTAMNELSLEDIHELIEEARELKKGKSDSVLSGKFAANLFFEPSTRTRFSFEVAEKKLGMNVLNLDGVSTSVQKGES
LYDTVKTLESIGADVCVIRHSHDHYYDELIGHVGIPVINAGDGCGQHPTQSLLDLMTIHEEWGRFAGLTVSIHGDIKHSR
VARSNAEVLTRLGAKVLFSGPDEWRDEDNPYGTYVSPDEAVAHSDVVMLLRIQHERHEKKAAERDYLETFGLSLKRAELL
KKDAVIMHPAPVNRGVEIDSALVESGRSRIFKQMENGVYIRMAVLKRALLNGENKKRGDQAYVLFN

Sequences:

>Translated_306_residues
MKHLTAMNELSLEDIHELIEEARELKKGKSDSVLSGKFAANLFFEPSTRTRFSFEVAEKKLGMNVLNLDGVSTSVQKGES
LYDTVKTLESIGADVCVIRHSHDHYYDELIGHVGIPVINAGDGCGQHPTQSLLDLMTIHEEWGRFAGLTVSIHGDIKHSR
VARSNAEVLTRLGAKVLFSGPDEWRDEDNPYGTYVSPDEAVAHSDVVMLLRIQHERHEKKAAERDYLETFGLSLKRAELL
KKDAVIMHPAPVNRGVEIDSALVESGRSRIFKQMENGVYIRMAVLKRALLNGENKKRGDQAYVLFN
>Mature_306_residues
MKHLTAMNELSLEDIHELIEEARELKKGKSDSVLSGKFAANLFFEPSTRTRFSFEVAEKKLGMNVLNLDGVSTSVQKGES
LYDTVKTLESIGADVCVIRHSHDHYYDELIGHVGIPVINAGDGCGQHPTQSLLDLMTIHEEWGRFAGLTVSIHGDIKHSR
VARSNAEVLTRLGAKVLFSGPDEWRDEDNPYGTYVSPDEAVAHSDVVMLLRIQHERHEKKAAERDYLETFGLSLKRAELL
KKDAVIMHPAPVNRGVEIDSALVESGRSRIFKQMENGVYIRMAVLKRALLNGENKKRGDQAYVLFN

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=307, Percent_Identity=38.1107491856677, Blast_Score=178, Evalue=5e-45,
Organism=Homo sapiens, GI38788445, Length=303, Percent_Identity=26.7326732673267, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI2367364, Length=307, Percent_Identity=34.2019543973941, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1790703, Length=332, Percent_Identity=25.9036144578313, Blast_Score=61, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI193204318, Length=290, Percent_Identity=36.2068965517241, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322331, Length=304, Percent_Identity=31.5789473684211, Blast_Score=150, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24642586, Length=312, Percent_Identity=35.5769230769231, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_BACLD (Q65JV0)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_078942.1
- RefSeq:   YP_091357.1
- ProteinModelPortal:   Q65JV0
- SMR:   Q65JV0
- STRING:   Q65JV0
- EnsemblBacteria:   EBBACT00000055756
- EnsemblBacteria:   EBBACT00000059065
- GeneID:   3030919
- GeneID:   3097708
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01769
- KEGG:   bli:BL02273
- NMPDR:   fig|279010.5.peg.1711
- eggNOG:   COG0540
- GeneTree:   EBGT00050000002562
- HOGENOM:   HBG579429
- OMA:   LYFAGPE
- ProtClustDB:   PRK00856
- BioCyc:   BLIC279010-1:BLI01769-MONOMER
- BioCyc:   BLIC279010:BL02273-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34264; Mature: 34264

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLTAMNELSLEDIHELIEEARELKKGKSDSVLSGKFAANLFFEPSTRTRFSFEVAEKK
CCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEEECCCCCCEEHHHHHHHH
LGMNVLNLDGVSTSVQKGESLYDTVKTLESIGADVCVIRHSHDHYYDELIGHVGIPVINA
HCCEEEECCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCEEEC
GDGCGQHPTQSLLDLMTIHEEWGRFAGLTVSIHGDIKHSRVARSNAEVLTRLGAKVLFSG
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCEEECC
PDEWRDEDNPYGTYVSPDEAVAHSDVVMLLRIQHERHEKKAAERDYLETFGLSLKRAELL
CHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KKDAVIMHPAPVNRGVEIDSALVESGRSRIFKQMENGVYIRMAVLKRALLNGENKKRGDQ
HCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCCCCCC
AYVLFN
EEEEEC
>Mature Secondary Structure
MKHLTAMNELSLEDIHELIEEARELKKGKSDSVLSGKFAANLFFEPSTRTRFSFEVAEKK
CCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEEECCCCCCEEHHHHHHHH
LGMNVLNLDGVSTSVQKGESLYDTVKTLESIGADVCVIRHSHDHYYDELIGHVGIPVINA
HCCEEEECCCCCHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCEEEC
GDGCGQHPTQSLLDLMTIHEEWGRFAGLTVSIHGDIKHSRVARSNAEVLTRLGAKVLFSG
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCEEECC
PDEWRDEDNPYGTYVSPDEAVAHSDVVMLLRIQHERHEKKAAERDYLETFGLSLKRAELL
CHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KKDAVIMHPAPVNRGVEIDSALVESGRSRIFKQMENGVYIRMAVLKRALLNGENKKRGDQ
HCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHHHHCCCCCCCCCC
AYVLFN
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA