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The map label for this gene is sigG [H]

Identifier: 52785510

GI number: 52785510

Start: 1711133

End: 1711912

Strand: Direct

Name: sigG [H]

Synonym: BLi01751

Alternate gene names: 52785510

Gene position: 1711133-1711912 (Clockwise)

Preceding gene: 52785509

Following gene: 52785511

Centisome position: 40.52

GC content: 44.87

Gene sequence:

>780_bases
GTGTCGAGGAATAAAGTTGAAATCTGCGGGGTTGATACATCAAAACTGCCCGTACTGAAGAACGAAGAGATGAGAAAGTT
ATTCAAGCAAATGCAGGATGAAGGAGACACAACAGCGAGAGAAAAGCTTGTTAACGGCAATTTGCGCCTTGTCTTAAGCG
TCATTCAAAGGTTTAACAACAGAGGAGAATATGTTGATGACTTATTCCAAGTCGGCTGCATCGGACTAATGAAATCAATT
GATAATTTTGACCTGAGCCACAATGTTAAGTTTTCAACATATGCTGTACCAATGATCATCGGAGAAATCCGCAGATATTT
GCGCGATAACAACCCGATCCGCGTCTCACGGTCACTCAGGGATATCGCGTACAAGGCGCTGCAAGTGAGAGAACGGCTGA
TCAGTGAGACAAGCAGGGAGCCGACTGCTCAGGAGATCGCTAAAGAGCTTGAAGTGTCCCATGAAGAAATCGTTTTTGCG
CTTGACGCCATTCAAGATCCTGTATCTTTGTTTGAGCCGATTTACAATGACGGAGGAGATCCGATTTATGTCATGGATCA
AATCAGCGATGAGCGCAACAAGGATTCGCAATGGATCGAAGAGCTCGCCTTAAAAGAAGGCATGAGACGGCTGAATGAAC
GGGAAAAAATGATCCTCAGAAAACGCTTCTTCCAAGGCAAGACGCAAATGGAAGTCGCTGATGAGATCGGAATCTCCCAA
GCCCAGGTCTCAAGGCTGGAAAAAGCCGCCATTAAACAAATGAATAAGAACATTCACTAA

Upstream 100 bases:

>100_bases
TCGGCTCGATCTTCTTATTTTTGGAAATTAACGATGCATATTTTTCCCACCCGAGGAGATACTGAACTTTGTACAGCAGC
TCCTGTAGGGAGGGAAAAAA

Downstream 100 bases:

>100_bases
GCCGCAGTCAATCTGCGGCTTTTCACATGCTAAAAATGGAAAATCTCTCATTCACTTTATTAAATATATAAGCTATAATA
AAAGAATTATATTTTAATTT

Product: sporulation sigma factor SigG

Products: NA

Alternate protein names: Stage III sporulation protein G [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSRNKVEICGVDTSKLPVLKNEEMRKLFKQMQDEGDTTAREKLVNGNLRLVLSVIQRFNNRGEYVDDLFQVGCIGLMKSI
DNFDLSHNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVRERLISETSREPTAQEIAKELEVSHEEIVFA
LDAIQDPVSLFEPIYNDGGDPIYVMDQISDERNKDSQWIEELALKEGMRRLNEREKMILRKRFFQGKTQMEVADEIGISQ
AQVSRLEKAAIKQMNKNIH

Sequences:

>Translated_259_residues
MSRNKVEICGVDTSKLPVLKNEEMRKLFKQMQDEGDTTAREKLVNGNLRLVLSVIQRFNNRGEYVDDLFQVGCIGLMKSI
DNFDLSHNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVRERLISETSREPTAQEIAKELEVSHEEIVFA
LDAIQDPVSLFEPIYNDGGDPIYVMDQISDERNKDSQWIEELALKEGMRRLNEREKMILRKRFFQGKTQMEVADEIGISQ
AQVSRLEKAAIKQMNKNIH
>Mature_258_residues
SRNKVEICGVDTSKLPVLKNEEMRKLFKQMQDEGDTTAREKLVNGNLRLVLSVIQRFNNRGEYVDDLFQVGCIGLMKSID
NFDLSHNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLRDIAYKALQVRERLISETSREPTAQEIAKELEVSHEEIVFAL
DAIQDPVSLFEPIYNDGGDPIYVMDQISDERNKDSQWIEELALKEGMRRLNEREKMILRKRFFQGKTQMEVADEIGISQA
QVSRLEKAAIKQMNKNIH

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes in the forespore [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=226, Percent_Identity=30.9734513274336, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789098, Length=253, Percent_Identity=26.0869565217391, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789871, Length=262, Percent_Identity=27.8625954198473, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014212
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 30024; Mature: 29893

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNKVEICGVDTSKLPVLKNEEMRKLFKQMQDEGDTTAREKLVNGNLRLVLSVIQRFNN
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCC
RGEYVDDLFQVGCIGLMKSIDNFDLSHNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHH
DIAYKALQVRERLISETSREPTAQEIAKELEVSHEEIVFALDAIQDPVSLFEPIYNDGGD
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHCCCCC
PIYVMDQISDERNKDSQWIEELALKEGMRRLNEREKMILRKRFFQGKTQMEVADEIGISQ
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCH
AQVSRLEKAAIKQMNKNIH
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRNKVEICGVDTSKLPVLKNEEMRKLFKQMQDEGDTTAREKLVNGNLRLVLSVIQRFNN
CCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCC
RGEYVDDLFQVGCIGLMKSIDNFDLSHNVKFSTYAVPMIIGEIRRYLRDNNPIRVSRSLR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHH
DIAYKALQVRERLISETSREPTAQEIAKELEVSHEEIVFALDAIQDPVSLFEPIYNDGGD
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHCCCCC
PIYVMDQISDERNKDSQWIEELALKEGMRRLNEREKMILRKRFFQGKTQMEVADEIGISQ
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCH
AQVSRLEKAAIKQMNKNIH
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2497052; 2459711; 9384377 [H]