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The map label for this gene is pdhD [H]

Identifier: 52785437

GI number: 52785437

Start: 1636215

End: 1637627

Strand: Direct

Name: pdhD [H]

Synonym: BLi01677

Alternate gene names: 52785437

Gene position: 1636215-1637627 (Clockwise)

Preceding gene: 52785436

Following gene: 52785442

Centisome position: 38.75

GC content: 47.28

Gene sequence:

>1413_bases
ATGGTAGTAGGAGATTTCCCTATTGAAACAGATACTCTTGTCATTGGTGCGGGACCTGGCGGCTATGTAGCTGCCATCCG
CGCTGCTCAGCTTGGACAAAAAGTAACAATCGTCGAAAAAGGCAATCTTGGAGGCGTATGTCTGAATGTCGGATGTATCC
CTTCAAAAGCGCTTATCAATGCAGGCCACCGCTATGAGAATGCGAAGCATTCTGAAGAGATGGGGATCACTGCTGAAAAC
GTAAAAGTTGACTTTACAAAGGTTCAAGAATGGAAAGCCTCTGTCGTTAATAAGCTTACCGGCGGTGTTGAAGGCCTTCT
GAAAGGAAACAAAGTCGACATCGTAAAAGGCGAAGCATACTTTGTAGACAGCAATTCTGTACGCGTGATGGATGAGAACT
CAGCTCAGACTTACACGTTCAAAAATGCGATCATTGCAACAGGTTCTCGCCCTATCGAATTGCCAAACTTCAAATATAGC
GACCGCGTACTGAATTCAACAGGCGCACTTGCACTGAAAGAAATTCCTAAGAAGCTCGTTGTCATCGGCGGCGGCTACAT
CGGAACAGAGCTCGGTACTGCATATGCAAACTTCGGTACTGAAGTTGTCATTCTTGAAGGCGGAGAAGAAATCCTTCCAG
GATTTGAGAAGCAAATGAGCTCTCTTGTGAAACGCAACCTGAAGAAAAAAGGCAACGTTGAAATCCATACAAAAGCAATG
GCTAAAGGCGTAGAAGAAAAAGCTGACGGCGTAACCGTTACGTTCGAAGTGAAAGGCGAAGAGCAAACGATCGATGCGGA
CTACGTACTTGTCACTGTCGGCCGCGTTGCAAACACGGACGAGCTCGGACTTGAACAAGTCGGCGTCGAAATGACGGACC
GCGGCATCATCAAAACGGACAAACAATGCCGTACAAACATTCCGAACATCTATGCGATCGGTGACATCATCGAAGGTCCT
CCGCTTGCGCATAAAGCTTCTTATGAAGGTAAAATTGCTGCTGAAGCGATTGCCGGAGAAGCTGCGGAAATCGACTATCT
TGGAATTCCTGCGGTCGTATTCTCTGAGCCTGAACTTGCATCTGTAGGCTACACTGAAGCACAGGCTAAAGAAGAAGGTC
TTTCAGTGACTGCAGCCAAATTCCCGTTTGCAGCAAACGGACGTGCGCTGTCATTAAACGAAACTGACGGTTTCCTTAAA
CTTGTAACACGCAAAGAAGACGGCTTAGTCATCGGTGCACAAATCGCCGGAGCAAGCGCTTCTGACATGATTTCTGAGCT
GAGCTTGGCAATCGAAGCAGGAATGACTGCTGAAGATATCGCAATGACAATCCACGCTCACCCTACATTGGGAGAAATCA
CAATGGAAGCTGCAGAAGTTGCCATCGGCATGCCGATCCACGTTGTAAAATAA

Upstream 100 bases:

>100_bases
GACCACCGTATGATCGACGGCGCAACTGCACAAAACGCGTTGAATCACATCAAGCGTTTGCTGAACGATCCACAATTAAT
TTTAATGGAGGCGTAATGTA

Downstream 100 bases:

>100_bases
ATCAAAAACCCCCTCTGCAGGGGGTTTTTTATGTTTTCTCTTATTCGGGTGCATGACAAATTGCCCGGGATACATGTGAA
GCAGAAAAAAAATCCCCTTG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; S complex, 50 kDa subunit [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MVVGDFPIETDTLVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLGGVCLNVGCIPSKALINAGHRYENAKHSEEMGITAEN
VKVDFTKVQEWKASVVNKLTGGVEGLLKGNKVDIVKGEAYFVDSNSVRVMDENSAQTYTFKNAIIATGSRPIELPNFKYS
DRVLNSTGALALKEIPKKLVVIGGGYIGTELGTAYANFGTEVVILEGGEEILPGFEKQMSSLVKRNLKKKGNVEIHTKAM
AKGVEEKADGVTVTFEVKGEEQTIDADYVLVTVGRVANTDELGLEQVGVEMTDRGIIKTDKQCRTNIPNIYAIGDIIEGP
PLAHKASYEGKIAAEAIAGEAAEIDYLGIPAVVFSEPELASVGYTEAQAKEEGLSVTAAKFPFAANGRALSLNETDGFLK
LVTRKEDGLVIGAQIAGASASDMISELSLAIEAGMTAEDIAMTIHAHPTLGEITMEAAEVAIGMPIHVVK

Sequences:

>Translated_470_residues
MVVGDFPIETDTLVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLGGVCLNVGCIPSKALINAGHRYENAKHSEEMGITAEN
VKVDFTKVQEWKASVVNKLTGGVEGLLKGNKVDIVKGEAYFVDSNSVRVMDENSAQTYTFKNAIIATGSRPIELPNFKYS
DRVLNSTGALALKEIPKKLVVIGGGYIGTELGTAYANFGTEVVILEGGEEILPGFEKQMSSLVKRNLKKKGNVEIHTKAM
AKGVEEKADGVTVTFEVKGEEQTIDADYVLVTVGRVANTDELGLEQVGVEMTDRGIIKTDKQCRTNIPNIYAIGDIIEGP
PLAHKASYEGKIAAEAIAGEAAEIDYLGIPAVVFSEPELASVGYTEAQAKEEGLSVTAAKFPFAANGRALSLNETDGFLK
LVTRKEDGLVIGAQIAGASASDMISELSLAIEAGMTAEDIAMTIHAHPTLGEITMEAAEVAIGMPIHVVK
>Mature_470_residues
MVVGDFPIETDTLVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLGGVCLNVGCIPSKALINAGHRYENAKHSEEMGITAEN
VKVDFTKVQEWKASVVNKLTGGVEGLLKGNKVDIVKGEAYFVDSNSVRVMDENSAQTYTFKNAIIATGSRPIELPNFKYS
DRVLNSTGALALKEIPKKLVVIGGGYIGTELGTAYANFGTEVVILEGGEEILPGFEKQMSSLVKRNLKKKGNVEIHTKAM
AKGVEEKADGVTVTFEVKGEEQTIDADYVLVTVGRVANTDELGLEQVGVEMTDRGIIKTDKQCRTNIPNIYAIGDIIEGP
PLAHKASYEGKIAAEAIAGEAAEIDYLGIPAVVFSEPELASVGYTEAQAKEEGLSVTAAKFPFAANGRALSLNETDGFLK
LVTRKEDGLVIGAQIAGASASDMISELSLAIEAGMTAEDIAMTIHAHPTLGEITMEAAEVAIGMPIHVVK

Specific function: Catalyzes the oxidation of dihydrolipoamide to lipoamide [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=467, Percent_Identity=44.5396145610278, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI50301238, Length=458, Percent_Identity=29.0393013100437, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI22035672, Length=461, Percent_Identity=30.1518438177874, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI291045266, Length=458, Percent_Identity=28.3842794759825, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI148277065, Length=454, Percent_Identity=26.6519823788546, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI33519430, Length=454, Percent_Identity=26.6519823788546, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI33519428, Length=454, Percent_Identity=26.6519823788546, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI33519426, Length=454, Percent_Identity=26.6519823788546, Blast_Score=145, Evalue=9e-35,
Organism=Homo sapiens, GI148277071, Length=454, Percent_Identity=26.6519823788546, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI291045268, Length=450, Percent_Identity=26.6666666666667, Blast_Score=122, Evalue=6e-28,
Organism=Escherichia coli, GI1786307, Length=458, Percent_Identity=48.2532751091703, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1789915, Length=440, Percent_Identity=30.2272727272727, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI87082354, Length=459, Percent_Identity=29.6296296296296, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI87081717, Length=447, Percent_Identity=29.3064876957494, Blast_Score=186, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI32565766, Length=462, Percent_Identity=42.6406926406926, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI71983429, Length=455, Percent_Identity=30.7692307692308, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71983419, Length=455, Percent_Identity=30.7692307692308, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17557007, Length=471, Percent_Identity=28.8747346072187, Blast_Score=156, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71982272, Length=488, Percent_Identity=26.2295081967213, Blast_Score=121, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=473, Percent_Identity=39.9577167019027, Blast_Score=309, Evalue=7e-85,
Organism=Saccharomyces cerevisiae, GI6325166, Length=466, Percent_Identity=29.8283261802575, Blast_Score=190, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6325240, Length=477, Percent_Identity=27.6729559748428, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI21358499, Length=470, Percent_Identity=45.1063829787234, Blast_Score=348, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24640549, Length=465, Percent_Identity=28.6021505376344, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24640553, Length=465, Percent_Identity=28.6021505376344, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24640551, Length=465, Percent_Identity=28.6021505376344, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI17737741, Length=472, Percent_Identity=26.0593220338983, Blast_Score=131, Evalue=1e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49681; Mature: 49681

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVGDFPIETDTLVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLGGVCLNVGCIPSKALIN
CEECCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCHHHHC
AGHRYENAKHSEEMGITAENVKVDFTKVQEWKASVVNKLTGGVEGLLKGNKVDIVKGEAY
CCCCCCCCCCHHHCCCEECCEEEEHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCEE
FVDSNSVRVMDENSAQTYTFKNAIIATGSRPIELPNFKYSDRVLNSTGALALKEIPKKLV
EEECCCEEEEECCCCEEEEECCEEEECCCCCEECCCCCCCCHHHCCCCCHHHHHCCCEEE
VIGGGYIGTELGTAYANFGTEVVILEGGEEILPGFEKQMSSLVKRNLKKKGNVEIHTKAM
EEECCEECHHHHHHHHCCCCEEEEEECCHHHCCCHHHHHHHHHHHHHHHCCCEEEEEHHH
AKGVEEKADGVTVTFEVKGEEQTIDADYVLVTVGRVANTDELGLEQVGVEMTDRGIIKTD
HCCCHHHCCCEEEEEEECCCCEEECCCEEEEEECCCCCCCCCCHHHHCCEECCCCCEECC
KQCRTNIPNIYAIGDIIEGPPLAHKASYEGKIAAEAIAGEAAEIDYLGIPAVVFSEPELA
HHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEHHHHCCCCCEEEEECCCEEEECCCCCC
SVGYTEAQAKEEGLSVTAAKFPFAANGRALSLNETDGFLKLVTRKEDGLVIGAQIAGASA
CCCCCHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCEEEEEEECCCCEEEEEEECCCCH
SDMISELSLAIEAGMTAEDIAMTIHAHPTLGEITMEAAEVAIGMPIHVVK
HHHHHHHHHHHHCCCCHHHEEEEEECCCCHHHHHHHHHHHEECCEEEEEC
>Mature Secondary Structure
MVVGDFPIETDTLVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLGGVCLNVGCIPSKALIN
CEECCCCCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCHHHHC
AGHRYENAKHSEEMGITAENVKVDFTKVQEWKASVVNKLTGGVEGLLKGNKVDIVKGEAY
CCCCCCCCCCHHHCCCEECCEEEEHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCEE
FVDSNSVRVMDENSAQTYTFKNAIIATGSRPIELPNFKYSDRVLNSTGALALKEIPKKLV
EEECCCEEEEECCCCEEEEECCEEEECCCCCEECCCCCCCCHHHCCCCCHHHHHCCCEEE
VIGGGYIGTELGTAYANFGTEVVILEGGEEILPGFEKQMSSLVKRNLKKKGNVEIHTKAM
EEECCEECHHHHHHHHCCCCEEEEEECCHHHCCCHHHHHHHHHHHHHHHCCCEEEEEHHH
AKGVEEKADGVTVTFEVKGEEQTIDADYVLVTVGRVANTDELGLEQVGVEMTDRGIIKTD
HCCCHHHCCCEEEEEEECCCCEEECCCEEEEEECCCCCCCCCCHHHHCCEECCCCCEECC
KQCRTNIPNIYAIGDIIEGPPLAHKASYEGKIAAEAIAGEAAEIDYLGIPAVVFSEPELA
HHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEEHHHHCCCCCEEEEECCCEEEECCCCCC
SVGYTEAQAKEEGLSVTAAKFPFAANGRALSLNETDGFLKLVTRKEDGLVIGAQIAGASA
CCCCCHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCEEEEEEECCCCEEEEEEECCCCH
SDMISELSLAIEAGMTAEDIAMTIHAHPTLGEITMEAAEVAIGMPIHVVK
HHHHHHHHHHHHCCCCHHHEEEEEECCCCHHHHHHHHHHHEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377; 1936936 [H]