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The map label for this gene is pdhC [H]

Identifier: 52785436

GI number: 52785436

Start: 1634918

End: 1636210

Strand: Direct

Name: pdhC [H]

Synonym: BLi01676

Alternate gene names: 52785436

Gene position: 1634918-1636210 (Clockwise)

Preceding gene: 52785435

Following gene: 52785437

Centisome position: 38.72

GC content: 47.1

Gene sequence:

>1293_bases
GTGGCATTTGAATTTAAACTTCCGGATATCGGGGAAGGAATCCACGAAGGCGAAATCGTGAAATGGTTCGTCAAGCCAAA
CGACGAGGTAAACGAAGATGACGTTTTGGCAGAGGTACAAAACGATAAAGCAGTTGTAGAAATTCCTTCACCTGTTAAAG
GAAAAGTATTAGAATTAAAAGTTGAAGAGGGAACAGTCGCAACCGTTGGGCAGACGATCATCACATTTGATGCACCAGGT
TACGAAGATCTTCAATTCAAAGGCGACGAATCAGGCGAAGCGAAAGCGGAAGAAGCTGAAAAACAAGAAACTGATGCACC
TGCAGAAGCAGCGGAAGCAAACGAGCAAGCGGATGCAGATCCGAATAAGCGCGTCATTGCAATGCCATCTGTTCGCAAGT
ATGCCCGCGAAAAAGGCGTTGATATCGTGAATGTTTCAGGTTCCGGCAAAAACGGCCGCGTTCTGAAAGAAGATATCGAC
AGCTTCTTAAACGGAGGAACTGCCGGAGACGCTAAGGCTGCTCAAGCTGAAGAGAAAGCAGAACCTGCTGCACAGCAACC
TGCAGCTGCGGTTCAAGTGCCTGAAGGCGAATTCCCTGAAACTCGCGAGAAAATGAGCGGAATCCGCAAAGCCATTGCAA
AAGCTATGGTCAACTCAAAACATACCGCTCCACACGTTACATTAATGGACGAAGTCGATGTGACAAACCTTGTCGCTCAC
CGCAAGCAGTTTAAACAAGTTGCGGCTGATCAAGGAATTAAGCTGACTTACCTGCCTTACGTGGTAAAAGCTTTGACATC
AGCTCTGAAAAAATATCCAGTCCTGAATACATCAATTGACGACAATACAGATGAAGTGATTCAGAAGCACTACTACAACA
TCGGTATTGCGGCAGACACTGAAAAAGGCCTGCTTGTGCCGGTTGTTAAAAATGCGGACCGCAAAGCGATTTTTGAGATT
TCAAACGAAATCAACGAGCTTGCGACAAAAGCACGCGAAGGCAAACTTGCTCCTGCTGAAATGAAAGGCGCTTCTTGCAC
AATTACGAATATCGGCTCTGCCGGCGGTCAATGGTTCACACCGGTTATCAACCATCCGGAAGTTGCGATCCTTGGAATCG
GCCGCATCGCTGAGAAAGCTGTCGTTCGTGACGGCGAAATCGTCGCTGCACCTGTTCTGGCTCTTTCTCTAAGCTTTGAC
CACCGTATGATCGACGGCGCAACTGCACAAAACGCGTTGAATCACATCAAGCGTTTGCTGAACGATCCACAATTAATTTT
AATGGAGGCGTAA

Upstream 100 bases:

>100_bases
ACAGTCAGTCGGAGAAACTGTAGCCGTGTACTGACAGTCGGCCTAAACAAGAGGCCGACCACTATATCAACTACGTAATA
ATCTTAGGAGGTCGAGAACT

Downstream 100 bases:

>100_bases
TGTAATGGTAGTAGGAGATTTCCCTATTGAAACAGATACTCTTGTCATTGGTGCGGGACCTGGCGGCTATGTAGCTGCCA
TCCGCGCTGCTCAGCTTGGA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MAFEFKLPDIGEGIHEGEIVKWFVKPNDEVNEDDVLAEVQNDKAVVEIPSPVKGKVLELKVEEGTVATVGQTIITFDAPG
YEDLQFKGDESGEAKAEEAEKQETDAPAEAAEANEQADADPNKRVIAMPSVRKYAREKGVDIVNVSGSGKNGRVLKEDID
SFLNGGTAGDAKAAQAEEKAEPAAQQPAAAVQVPEGEFPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTNLVAH
RKQFKQVAADQGIKLTYLPYVVKALTSALKKYPVLNTSIDDNTDEVIQKHYYNIGIAADTEKGLLVPVVKNADRKAIFEI
SNEINELATKAREGKLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVAILGIGRIAEKAVVRDGEIVAAPVLALSLSFD
HRMIDGATAQNALNHIKRLLNDPQLILMEA

Sequences:

>Translated_430_residues
MAFEFKLPDIGEGIHEGEIVKWFVKPNDEVNEDDVLAEVQNDKAVVEIPSPVKGKVLELKVEEGTVATVGQTIITFDAPG
YEDLQFKGDESGEAKAEEAEKQETDAPAEAAEANEQADADPNKRVIAMPSVRKYAREKGVDIVNVSGSGKNGRVLKEDID
SFLNGGTAGDAKAAQAEEKAEPAAQQPAAAVQVPEGEFPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTNLVAH
RKQFKQVAADQGIKLTYLPYVVKALTSALKKYPVLNTSIDDNTDEVIQKHYYNIGIAADTEKGLLVPVVKNADRKAIFEI
SNEINELATKAREGKLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVAILGIGRIAEKAVVRDGEIVAAPVLALSLSFD
HRMIDGATAQNALNHIKRLLNDPQLILMEA
>Mature_429_residues
AFEFKLPDIGEGIHEGEIVKWFVKPNDEVNEDDVLAEVQNDKAVVEIPSPVKGKVLELKVEEGTVATVGQTIITFDAPGY
EDLQFKGDESGEAKAEEAEKQETDAPAEAAEANEQADADPNKRVIAMPSVRKYAREKGVDIVNVSGSGKNGRVLKEDIDS
FLNGGTAGDAKAAQAEEKAEPAAQQPAAAVQVPEGEFPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTNLVAHR
KQFKQVAADQGIKLTYLPYVVKALTSALKKYPVLNTSIDDNTDEVIQKHYYNIGIAADTEKGLLVPVVKNADRKAIFEIS
NEINELATKAREGKLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVAILGIGRIAEKAVVRDGEIVAAPVLALSLSFDH
RMIDGATAQNALNHIKRLLNDPQLILMEA

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=434, Percent_Identity=34.7926267281106, Blast_Score=248, Evalue=8e-66,
Organism=Homo sapiens, GI31711992, Length=441, Percent_Identity=30.6122448979592, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI19923748, Length=250, Percent_Identity=35.2, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI203098753, Length=447, Percent_Identity=26.1744966442953, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI203098816, Length=447, Percent_Identity=26.1744966442953, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=33.75, Blast_Score=89, Evalue=9e-18,
Organism=Escherichia coli, GI1786305, Length=433, Percent_Identity=34.8729792147806, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1786946, Length=430, Percent_Identity=31.8604651162791, Blast_Score=213, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI17537937, Length=430, Percent_Identity=34.8837209302326, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI25146366, Length=431, Percent_Identity=31.7865429234339, Blast_Score=194, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI17560088, Length=449, Percent_Identity=28.9532293986637, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17538894, Length=226, Percent_Identity=33.6283185840708, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=427, Percent_Identity=31.8501170960187, Blast_Score=200, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6324258, Length=446, Percent_Identity=29.5964125560538, Blast_Score=163, Evalue=5e-41,
Organism=Drosophila melanogaster, GI18859875, Length=434, Percent_Identity=38.0184331797235, Blast_Score=258, Evalue=8e-69,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=32.8947368421053, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=32.8947368421053, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24645909, Length=233, Percent_Identity=33.4763948497854, Blast_Score=131, Evalue=1e-30,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46357; Mature: 46226

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFEFKLPDIGEGIHEGEIVKWFVKPNDEVNEDDVLAEVQNDKAVVEIPSPVKGKVLELK
CCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHEECCCCEEEECCCCCCCCEEEEE
VEEGTVATVGQTIITFDAPGYEDLQFKGDESGEAKAEEAEKQETDAPAEAAEANEQADAD
ECCCCEEECCCEEEEECCCCCCCCEECCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCC
PNKRVIAMPSVRKYAREKGVDIVNVSGSGKNGRVLKEDIDSFLNGGTAGDAKAAQAEEKA
CCCEEEECCHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHC
EPAAQQPAAAVQVPEGEFPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTNLVAH
CCHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHH
RKQFKQVAADQGIKLTYLPYVVKALTSALKKYPVLNTSIDDNTDEVIQKHYYNIGIAADT
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEEEECC
EKGLLVPVVKNADRKAIFEISNEINELATKAREGKLAPAEMKGASCTITNIGSAGGQWFT
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEEEECCCCCCCEEC
PVINHPEVAILGIGRIAEKAVVRDGEIVAAPVLALSLSFDHRMIDGATAQNALNHIKRLL
CCCCCCCEEEEECCHHHHHHHHCCCCEEEECHHHEEECCCCCCCCCHHHHHHHHHHHHHH
NDPQLILMEA
CCCCEEEEEC
>Mature Secondary Structure 
AFEFKLPDIGEGIHEGEIVKWFVKPNDEVNEDDVLAEVQNDKAVVEIPSPVKGKVLELK
CEEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHEECCCCEEEECCCCCCCCEEEEE
VEEGTVATVGQTIITFDAPGYEDLQFKGDESGEAKAEEAEKQETDAPAEAAEANEQADAD
ECCCCEEECCCEEEEECCCCCCCCEECCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCC
PNKRVIAMPSVRKYAREKGVDIVNVSGSGKNGRVLKEDIDSFLNGGTAGDAKAAQAEEKA
CCCEEEECCHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHC
EPAAQQPAAAVQVPEGEFPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTNLVAH
CCHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHH
RKQFKQVAADQGIKLTYLPYVVKALTSALKKYPVLNTSIDDNTDEVIQKHYYNIGIAADT
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEEEECC
EKGLLVPVVKNADRKAIFEISNEINELATKAREGKLAPAEMKGASCTITNIGSAGGQWFT
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEEEECCCCCCCEEC
PVINHPEVAILGIGRIAEKAVVRDGEIVAAPVLALSLSFDHRMIDGATAQNALNHIKRLL
CCCCCCCEEEEECCHHHHHHHHCCCCEEEECHHHEEECCCCCCCCCHHHHHHHHHHHHHH
NDPQLILMEA
CCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]