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The map label for this gene is pdhA [H]

Identifier: 52785434

GI number: 52785434

Start: 1632659

End: 1633774

Strand: Direct

Name: pdhA [H]

Synonym: BLi01674

Alternate gene names: 52785434

Gene position: 1632659-1633774 (Clockwise)

Preceding gene: 52785433

Following gene: 52785435

Centisome position: 38.66

GC content: 47.04

Gene sequence:

>1116_bases
ATGGCTGCAAAAACGAAAAAAGCAATCGTTGATAGCAAAAAGCAATTCGATGCCATTAAAAAGCAGTTCGAAACGTTTCA
AATTTTGAATGAAGAAGGCGAAGTCGTTAATGAAGCGGCAATGCCGGATCTATCTGATGATCAATTAAAAGAATTAATGC
GCCGCATGGTGTATACACGCATTTTGGATCAGCGTTCTATTTCTTTGAACAGACAAGGCCGCCTTGGCTTCTACGCGCCG
ACTGCCGGTCAGGAAGCTTCTCAAATTGCTACGCATTTCGCGCTTGAAAAAGAAGACTTCGTTCTTCCGGGCTACCGTGA
TGTTCCGCAGCTGATCTGGCACGGACTTCCGCTTTATCAAGCATTCTTGTTCTCTCGCGGACACTTCCGCGGCAACCAAA
TGCCTGACGATGTCAATGCGCTTTCACCGCAAATTATTATTGGTGCGCAATACATCCAAACGGCTGGCGTTGCACTCGGC
TTGAAAAAACGCGGCAAACAGGCTGTGGCGATCACCTATACTGGAGACGGCGGTGCTTCCCAAGGTGACTTCTACGAAGG
AATCAACTTTGCCGGTGCGTATAAAGCTCCAGCTATTTTCGTTGTTCAAAACAACCGCTATGCGATTTCAACACCTGTTG
AAAAGCAATCAGCAGCTCAAACAATCGCCCAAAAAGCTGCGGCTGCAGGTATCGTCGGCGTTCAAGTAGACGGAATGGAT
GCCCTTGCTGTATACGCTGCGACACGCGAAGCACGCGAGCGCGCTGTAAACGGCGAAGGCCCTACCCTGATCGAAACGCT
TTGCTTCCGTTACGGTCCTCATACTATGGCCGGTGACGATCCGACAAAATACCGTACAAAAGAAATCGAGAATGAGTGGG
AGCAGAAAGATCCGCTTGTCCGTTTCCGCAAGTTCTTGGAAAACAAAGGCCTTTGGTCTGAAGAAGAAGAAAACAAGGTA
ATTGAAGAAGCGAAAGAAGAAATCAAGCAGGCGATCAAAAAAGCTGACGAAGAGCCGAAGCAAAAAGTGACCGACCTGAT
CGACATTATGTATGAAGAGCTTCCTTACAACCTTGTAGAACAAAAGGAAATTTATAAAGAGAAGGAGTCGAAGTAA

Upstream 100 bases:

>100_bases
ATAAATATGAGTTGCGCCCGTCAATTTAGGACACCCTAATTCTGTATGTATAAGGGATACACGTCTATGACTGTCAAAAG
AAAGGAAGAGGTGACTTTAT

Downstream 100 bases:

>100_bases
GCCATGGCGCAAATGACAATGATTCAAGCAATCACTGATGCGTTACGCACAGAACTGAAAAATGACGAAAATGTCTTAGT
TTTCGGAGAAGACGTCGGTG

Product: PdhA

Products: NA

Alternate protein names: S complex, 42 kDa subunit; Vegetative protein 220; VEG220 [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MAAKTKKAIVDSKKQFDAIKKQFETFQILNEEGEVVNEAAMPDLSDDQLKELMRRMVYTRILDQRSISLNRQGRLGFYAP
TAGQEASQIATHFALEKEDFVLPGYRDVPQLIWHGLPLYQAFLFSRGHFRGNQMPDDVNALSPQIIIGAQYIQTAGVALG
LKKRGKQAVAITYTGDGGASQGDFYEGINFAGAYKAPAIFVVQNNRYAISTPVEKQSAAQTIAQKAAAAGIVGVQVDGMD
ALAVYAATREARERAVNGEGPTLIETLCFRYGPHTMAGDDPTKYRTKEIENEWEQKDPLVRFRKFLENKGLWSEEEENKV
IEEAKEEIKQAIKKADEEPKQKVTDLIDIMYEELPYNLVEQKEIYKEKESK

Sequences:

>Translated_371_residues
MAAKTKKAIVDSKKQFDAIKKQFETFQILNEEGEVVNEAAMPDLSDDQLKELMRRMVYTRILDQRSISLNRQGRLGFYAP
TAGQEASQIATHFALEKEDFVLPGYRDVPQLIWHGLPLYQAFLFSRGHFRGNQMPDDVNALSPQIIIGAQYIQTAGVALG
LKKRGKQAVAITYTGDGGASQGDFYEGINFAGAYKAPAIFVVQNNRYAISTPVEKQSAAQTIAQKAAAAGIVGVQVDGMD
ALAVYAATREARERAVNGEGPTLIETLCFRYGPHTMAGDDPTKYRTKEIENEWEQKDPLVRFRKFLENKGLWSEEEENKV
IEEAKEEIKQAIKKADEEPKQKVTDLIDIMYEELPYNLVEQKEIYKEKESK
>Mature_370_residues
AAKTKKAIVDSKKQFDAIKKQFETFQILNEEGEVVNEAAMPDLSDDQLKELMRRMVYTRILDQRSISLNRQGRLGFYAPT
AGQEASQIATHFALEKEDFVLPGYRDVPQLIWHGLPLYQAFLFSRGHFRGNQMPDDVNALSPQIIIGAQYIQTAGVALGL
KKRGKQAVAITYTGDGGASQGDFYEGINFAGAYKAPAIFVVQNNRYAISTPVEKQSAAQTIAQKAAAAGIVGVQVDGMDA
LAVYAATREARERAVNGEGPTLIETLCFRYGPHTMAGDDPTKYRTKEIENEWEQKDPLVRFRKFLENKGLWSEEEENKVI
EEAKEEIKQAIKKADEEPKQKVTDLIDIMYEELPYNLVEQKEIYKEKESK

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11386135, Length=348, Percent_Identity=32.183908045977, Blast_Score=201, Evalue=1e-51,
Organism=Homo sapiens, GI258645172, Length=348, Percent_Identity=32.183908045977, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI4505685, Length=331, Percent_Identity=29.9093655589124, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI291084742, Length=331, Percent_Identity=29.9093655589124, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI291084744, Length=211, Percent_Identity=36.9668246445498, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI4885543, Length=309, Percent_Identity=29.7734627831715, Blast_Score=128, Evalue=7e-30,
Organism=Homo sapiens, GI291084757, Length=314, Percent_Identity=29.6178343949045, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI86563357, Length=349, Percent_Identity=32.9512893982808, Blast_Score=201, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI86563355, Length=349, Percent_Identity=32.9512893982808, Blast_Score=201, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI17536047, Length=302, Percent_Identity=31.4569536423841, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI32564172, Length=302, Percent_Identity=31.4569536423841, Blast_Score=134, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6321026, Length=342, Percent_Identity=27.4853801169591, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21355903, Length=349, Percent_Identity=30.3724928366762, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24639744, Length=321, Percent_Identity=29.595015576324, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI28571106, Length=321, Percent_Identity=29.595015576324, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24639740, Length=321, Percent_Identity=29.595015576324, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24639746, Length=288, Percent_Identity=30.5555555555556, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24639748, Length=285, Percent_Identity=31.2280701754386, Blast_Score=119, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR017596 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 41713; Mature: 41582

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00037 MYB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKTKKAIVDSKKQFDAIKKQFETFQILNEEGEVVNEAAMPDLSDDQLKELMRRMVYTR
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHH
ILDQRSISLNRQGRLGFYAPTAGQEASQIATHFALEKEDFVLPGYRDVPQLIWHGLPLYQ
HHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHH
AFLFSRGHFRGNQMPDDVNALSPQIIIGAQYIQTAGVALGLKKRGKQAVAITYTGDGGAS
HHHHHCCCCCCCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCC
QGDFYEGINFAGAYKAPAIFVVQNNRYAISTPVEKQSAAQTIAQKAAAAGIVGVQVDGMD
CCCHHHCCCCCCCCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHHCCEEEEEECCCH
ALAVYAATREARERAVNGEGPTLIETLCFRYGPHTMAGDDPTKYRTKEIENEWEQKDPLV
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHH
RFRKFLENKGLWSEEEENKVIEEAKEEIKQAIKKADEEPKQKVTDLIDIMYEELPYNLVE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHH
QKEIYKEKESK
HHHHHHHHCCC
>Mature Secondary Structure 
AAKTKKAIVDSKKQFDAIKKQFETFQILNEEGEVVNEAAMPDLSDDQLKELMRRMVYTR
CCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHH
ILDQRSISLNRQGRLGFYAPTAGQEASQIATHFALEKEDFVLPGYRDVPQLIWHGLPLYQ
HHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCHHHH
AFLFSRGHFRGNQMPDDVNALSPQIIIGAQYIQTAGVALGLKKRGKQAVAITYTGDGGAS
HHHHHCCCCCCCCCCCCHHHCCCEEEEHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCC
QGDFYEGINFAGAYKAPAIFVVQNNRYAISTPVEKQSAAQTIAQKAAAAGIVGVQVDGMD
CCCHHHCCCCCCCCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHHCCEEEEEECCCH
ALAVYAATREARERAVNGEGPTLIETLCFRYGPHTMAGDDPTKYRTKEIENEWEQKDPLV
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHH
RFRKFLENKGLWSEEEENKVIEEAKEEIKQAIKKADEEPKQKVTDLIDIMYEELPYNLVE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHH
QKEIYKEKESK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377; 9298659 [H]