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The map label for this gene is ykrZ

Identifier: 52785282

GI number: 52785282

Start: 1492407

End: 1492946

Strand: Direct

Name: ykrZ

Synonym: BLi01517

Alternate gene names: 52785282

Gene position: 1492407-1492946 (Clockwise)

Preceding gene: 52785281

Following gene: 52785285

Centisome position: 35.34

GC content: 44.07

Gene sequence:

>540_bases
ATGGCGACAATTCGGATCCATGATGAACAAAACACGAGCATTGAAAACCAGGAAGAAGTAGAACAATTTCTAAAGCGGCA
GGAGGTCATTTATGAAAAGTGGGATATCACAAAACTGCCGGCCGGTTTAAAGGAAAAATACGATTTGACCGATGAAGAGA
AACAGGAGATTTTAGACGTCTTTGCCGCTGAAATCAAAGACATTTCAGAACGCCGCGGCTATAAAGCGAGCGATGTCATC
TCGCTTTCCGACCAAAATCCGAAACTCGATGAGCTTTTGAAAAACTTCAAACAGGAGCATCATCATACAGACGATGAAGT
CAGGTTTATCGTCAGCGGCCACGGCATCTTTGCGATAGAAGGAAAAGACGGCACATTTTTTGATGTCCTTTTAAATCCGG
GGGATTTGATCTCTGTTCCTGAAAACACCCGCCATTATTTCACGCTTCAGGAAGACCGGAAAGTCGTGGCTGTCAGAATC
TTTGTGACGACAGAGGGCTGGGTGCCGATTTATGAAAAAGAGAATGTGAACCAGTCATAA

Upstream 100 bases:

>100_bases
CAAAGCGGATGCTTGAAGCATATGAATTCTTGTTCAGCTGGCATTTAAAGCTGAAAGCTTTGCAGGCTTATCACGTTTAA
CTAAAAAAGGGGGAGAAAGC

Downstream 100 bases:

>100_bases
AAAAAGCGTCCCCGGGGACGCTTTTTATTTGTTTCTGTCATAAAGAGCCTTGATGTGGCCTTCATGAAATGAAAGATGCG
GTTTTTCATTTTGATAGTAT

Product: YkrZ

Products: NA

Alternate protein names: 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase; DHK-MTPene dioxygenase; Acireductone dioxygenase (Fe(2+)-requiring); ARD'; Fe-ARD; Acireductone dioxygenase (Ni(2+)-requiring); ARD; Ni-ARD

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MATIRIHDEQNTSIENQEEVEQFLKRQEVIYEKWDITKLPAGLKEKYDLTDEEKQEILDVFAAEIKDISERRGYKASDVI
SLSDQNPKLDELLKNFKQEHHHTDDEVRFIVSGHGIFAIEGKDGTFFDVLLNPGDLISVPENTRHYFTLQEDRKVVAVRI
FVTTEGWVPIYEKENVNQS

Sequences:

>Translated_179_residues
MATIRIHDEQNTSIENQEEVEQFLKRQEVIYEKWDITKLPAGLKEKYDLTDEEKQEILDVFAAEIKDISERRGYKASDVI
SLSDQNPKLDELLKNFKQEHHHTDDEVRFIVSGHGIFAIEGKDGTFFDVLLNPGDLISVPENTRHYFTLQEDRKVVAVRI
FVTTEGWVPIYEKENVNQS
>Mature_178_residues
ATIRIHDEQNTSIENQEEVEQFLKRQEVIYEKWDITKLPAGLKEKYDLTDEEKQEILDVFAAEIKDISERRGYKASDVIS
LSDQNPKLDELLKNFKQEHHHTDDEVRFIVSGHGIFAIEGKDGTFFDVLLNPGDLISVPENTRHYFTLQEDRKVVAVRIF
VTTEGWVPIYEKENVNQS

Specific function: Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4- me

COG id: COG1791

COG function: function code S; Uncharacterized conserved protein, contains double-stranded beta-helix domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acireductone dioxygenase (ARD) family

Homologues:

Organism=Homo sapiens, GI171460928, Length=107, Percent_Identity=32.7102803738318, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323651, Length=113, Percent_Identity=30.9734513274336, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161082987, Length=106, Percent_Identity=33.0188679245283, Blast_Score=66, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTND_BACLD (Q65KJ6)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_078702.1
- RefSeq:   YP_091111.1
- ProteinModelPortal:   Q65KJ6
- SMR:   Q65KJ6
- STRING:   Q65KJ6
- EnsemblBacteria:   EBBACT00000058032
- EnsemblBacteria:   EBBACT00000061009
- GeneID:   3030599
- GeneID:   3099177
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01517
- KEGG:   bli:BL03542
- NMPDR:   fig|279010.5.peg.1069
- eggNOG:   COG1791
- GeneTree:   EBGT00050000002186
- HOGENOM:   HBG660685
- OMA:   AGWEAIY
- ProtClustDB:   CLSK887193
- BioCyc:   BLIC279010-1:BLI01517-MONOMER
- BioCyc:   BLIC279010:BL03542-MONOMER
- HAMAP:   MF_01682
- InterPro:   IPR004313
- InterPro:   IPR011051
- InterPro:   IPR014710
- Gene3D:   G3DSA:2.60.120.10
- PANTHER:   PTHR23418

Pfam domain/function: PF03079 ARD; SSF51182 RmlC_like_cupin

EC number: =1.13.11.54; =1.13.11.53

Molecular weight: Translated: 20866; Mature: 20734

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIRIHDEQNTSIENQEEVEQFLKRQEVIYEKWDITKLPAGLKEKYDLTDEEKQEILDV
CEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
FAAEIKDISERRGYKASDVISLSDQNPKLDELLKNFKQEHHHTDDEVRFIVSGHGIFAIE
HHHHHHHHHHHCCCCCHHEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEE
GKDGTFFDVLLNPGDLISVPENTRHYFTLQEDRKVVAVRIFVTTEGWVPIYEKENVNQS
CCCCCEEEEEECCCCEEECCCCCCEEEEECCCCEEEEEEEEEEECCCEEEEECCCCCCC
>Mature Secondary Structure 
ATIRIHDEQNTSIENQEEVEQFLKRQEVIYEKWDITKLPAGLKEKYDLTDEEKQEILDV
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
FAAEIKDISERRGYKASDVISLSDQNPKLDELLKNFKQEHHHTDDEVRFIVSGHGIFAIE
HHHHHHHHHHHCCCCCHHEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEE
GKDGTFFDVLLNPGDLISVPENTRHYFTLQEDRKVVAVRIFVTTEGWVPIYEKENVNQS
CCCCCEEEEEECCCCEEECCCCCCEEEEECCCCEEEEEEEEEEECCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA