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The map label for this gene is kinE [H]

Identifier: 52785274

GI number: 52785274

Start: 1482088

End: 1484298

Strand: Direct

Name: kinE [H]

Synonym: BLi01509

Alternate gene names: 52785274

Gene position: 1482088-1484298 (Clockwise)

Preceding gene: 52785272

Following gene: 52785275

Centisome position: 35.1

GC content: 44.87

Gene sequence:

>2211_bases
ATGGAGGAAACTCTTGATTTGAATGAATCCTTAAATCTGCAAAACCAGCTGAACAGACTGAGGAAAGAAAACGCCAAGCT
GAAAGCGGATTTGTATCAGTATGAGCTGCTGTTTAACGGGACGCTGGACGCTATTTTTATATTGGATAAACAGATGAACA
TCATTAAGGCGAATGATGCCGCCTGCAAGATCCTGCAAAGCCAAAAAAAGAATTTGCTGAACCGGTCGGCGCTCGACTTC
CTGCACGCCGTTCCGCCGGATGAACTAAACCAGAACATTCACCGCTTCTTGGAGCGGGGATATGCGAAAAAAGAAGTGGC
CATCAGGCTTGACAACGGATCTGTCAAATATATCGAATTTACCGCAAGCAAAGGAATCGGTGAGGACTGCTTTTTTGTCG
TCATGAGAGACATTTCGTCTAAAAAAATATTGGAGCGGGAGCGCACCATCAATGAAAAGCTGTTTAAAGATTTGTTTCAC
CGCGCGGTTGAAGGAATCGTCATCTTTGATAAACAAGGCTGTTTCATCGATGCAAACCATTCGTTTTGCCAGAGCTTCGA
AATTGAAAAATCAGAGCTCTCAAAACTGAAGCTGTCTCAATTCGTATCGCCTGAAAACAAGGGGCGGCTCGATAAAATCT
GGAACGCTCTTTTCAGAAGAGGGAAGGCAAAAGGGGAGCTTCCTGTCCGCCTCAGATCAGGAGAAGACAGGCTGTTTGAG
CTGACGATAACCTCGAATATTTTAAATGGCTTTTACATGTCGATTATGAGGGATATTACCGAAAAGCGCTCAATGGAAAA
ACAGCTGTTTAAGAGCGAAGAACGCTTTAGGGAAGTCTTTGAAAATGCGATGGATGCCATCATTCTGATGAATAACGACG
GCCGCATCGTCAAAGCGAACCAGTCGGCCTGCAAAATCTTCGAACTGCCGATGGACCAGCTGCTCAACAAGAAGATGACT
GATTTTATAGATCGAACCGATCAGCGGTATCATGCCATTCAAAAGCAGTACGATCAAAAAGGGGAAATCCGGGCGGAACT
GCTGTTTCGTATGGCAAACGGACAATATAAGGAGCTTGAGTTCACATCAAAACGGATTATGTTCGACAGCCAGGACTTAA
CCATTTTGAGAAATGTCAGCGACAGAAAACGAATGGAACAAGAATTGCGGGAAAGCGAATTGAAGTTCAGAAAAGTATTC
AACGGATCAATGGACGGAATCGTTCTTTTCAACAACCAGTACGATATTATCGATGCGAACCCCCTTGCCGGAAAAATTCT
CAGCATCCCTCTTGAACAGCTGAAAACGTCCAATCTTCTTGATGTCATCTCCGGCTATCACATCGAAAACGCGGCTTCTC
CCGCTAAAACGATTTCCTTTGAAGAAATGGACAATGATATCCCATTCCTGCTGAGCGACCAAAAACGGATACTTGAATTT
TCATTTAAGCGAAACATCATTCAAAATATGAATCTGGCGATGCTCAGAGACGTGACGGAACGAAAAGAGCTTGAAGAGCG
GCTCCGCAAGTCTGATACCCTCCATGTCGTCGGAGAACTCGCAGCCGGAATTGCTCATGAAATCCGCAACCCGATGACGG
CATTAAAAGGATTTATCCAGCTGCTGAAAGGCAGCATCCAAAATGAGGATCACGCGCTTTACTTTAATGTCATCACATCA
GAGCTCAAAAGGATTGAATCCATTATTACGGAGTTTCTCATCCTTGCAAAACCGCAGGCGATTATGTATGAAGAAAAAAA
CATCGTCAAAATCATGCAGGACACAATGGATCTGCTGCATGCGCAGGCAAACCTTGACAACGTCCAGATGCATCTGAAGT
GCGAAGACGGCATTCCGCTGATCTACTGCGAGCCGAATCAGCTGAAGCAGGTATTTATCAACATTTTGAAAAATGCGATC
GAAGTAATGCCCGACGGAGGCAATGTATATGTGTCGATCAAACCGAAAGGAGACGACCATATCATTGTCTCGCTTGCTGA
CGAAGGAATCGGCATGACAGAAGACAAATTGAAGAGGCTCGGCGAACCTTTTTACACGACAAAAGAACGGGGCACTGGAC
TCGGGCTGATGGTCAGTTATAAAATTATTGAAGAACATAAAGGAAAAATCGAAGTTGAAAGTGAAGAAGGGAGAGGCACG
ACATTCCACCTGACTCTCCCGATCAGACAGCAGCATGAGGAGGAGGCTTAA

Upstream 100 bases:

>100_bases
AAAATAGGCGGAAAGGTTGTAAAAGTTTGAAACAAATGAAGGAAGATTTTCCGGTTTGTAGAATAATTGGAGGACAGGTT
TTCAAGAAGGAGTGTAATAA

Downstream 100 bases:

>100_bases
TCGCATCGTGGGCTTTTATATTGAAATGACCTCGCCTTTTGGACAAGCCTACATCGTCGAGGAAAATAACCATATTACAC
ATTTGCTATTTGAAAAAGAA

Product: KinE

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 736; Mature: 736

Protein sequence:

>736_residues
MEETLDLNESLNLQNQLNRLRKENAKLKADLYQYELLFNGTLDAIFILDKQMNIIKANDAACKILQSQKKNLLNRSALDF
LHAVPPDELNQNIHRFLERGYAKKEVAIRLDNGSVKYIEFTASKGIGEDCFFVVMRDISSKKILERERTINEKLFKDLFH
RAVEGIVIFDKQGCFIDANHSFCQSFEIEKSELSKLKLSQFVSPENKGRLDKIWNALFRRGKAKGELPVRLRSGEDRLFE
LTITSNILNGFYMSIMRDITEKRSMEKQLFKSEERFREVFENAMDAIILMNNDGRIVKANQSACKIFELPMDQLLNKKMT
DFIDRTDQRYHAIQKQYDQKGEIRAELLFRMANGQYKELEFTSKRIMFDSQDLTILRNVSDRKRMEQELRESELKFRKVF
NGSMDGIVLFNNQYDIIDANPLAGKILSIPLEQLKTSNLLDVISGYHIENAASPAKTISFEEMDNDIPFLLSDQKRILEF
SFKRNIIQNMNLAMLRDVTERKELEERLRKSDTLHVVGELAAGIAHEIRNPMTALKGFIQLLKGSIQNEDHALYFNVITS
ELKRIESIITEFLILAKPQAIMYEEKNIVKIMQDTMDLLHAQANLDNVQMHLKCEDGIPLIYCEPNQLKQVFINILKNAI
EVMPDGGNVYVSIKPKGDDHIIVSLADEGIGMTEDKLKRLGEPFYTTKERGTGLGLMVSYKIIEEHKGKIEVESEEGRGT
TFHLTLPIRQQHEEEA

Sequences:

>Translated_736_residues
MEETLDLNESLNLQNQLNRLRKENAKLKADLYQYELLFNGTLDAIFILDKQMNIIKANDAACKILQSQKKNLLNRSALDF
LHAVPPDELNQNIHRFLERGYAKKEVAIRLDNGSVKYIEFTASKGIGEDCFFVVMRDISSKKILERERTINEKLFKDLFH
RAVEGIVIFDKQGCFIDANHSFCQSFEIEKSELSKLKLSQFVSPENKGRLDKIWNALFRRGKAKGELPVRLRSGEDRLFE
LTITSNILNGFYMSIMRDITEKRSMEKQLFKSEERFREVFENAMDAIILMNNDGRIVKANQSACKIFELPMDQLLNKKMT
DFIDRTDQRYHAIQKQYDQKGEIRAELLFRMANGQYKELEFTSKRIMFDSQDLTILRNVSDRKRMEQELRESELKFRKVF
NGSMDGIVLFNNQYDIIDANPLAGKILSIPLEQLKTSNLLDVISGYHIENAASPAKTISFEEMDNDIPFLLSDQKRILEF
SFKRNIIQNMNLAMLRDVTERKELEERLRKSDTLHVVGELAAGIAHEIRNPMTALKGFIQLLKGSIQNEDHALYFNVITS
ELKRIESIITEFLILAKPQAIMYEEKNIVKIMQDTMDLLHAQANLDNVQMHLKCEDGIPLIYCEPNQLKQVFINILKNAI
EVMPDGGNVYVSIKPKGDDHIIVSLADEGIGMTEDKLKRLGEPFYTTKERGTGLGLMVSYKIIEEHKGKIEVESEEGRGT
TFHLTLPIRQQHEEEA
>Mature_736_residues
MEETLDLNESLNLQNQLNRLRKENAKLKADLYQYELLFNGTLDAIFILDKQMNIIKANDAACKILQSQKKNLLNRSALDF
LHAVPPDELNQNIHRFLERGYAKKEVAIRLDNGSVKYIEFTASKGIGEDCFFVVMRDISSKKILERERTINEKLFKDLFH
RAVEGIVIFDKQGCFIDANHSFCQSFEIEKSELSKLKLSQFVSPENKGRLDKIWNALFRRGKAKGELPVRLRSGEDRLFE
LTITSNILNGFYMSIMRDITEKRSMEKQLFKSEERFREVFENAMDAIILMNNDGRIVKANQSACKIFELPMDQLLNKKMT
DFIDRTDQRYHAIQKQYDQKGEIRAELLFRMANGQYKELEFTSKRIMFDSQDLTILRNVSDRKRMEQELRESELKFRKVF
NGSMDGIVLFNNQYDIIDANPLAGKILSIPLEQLKTSNLLDVISGYHIENAASPAKTISFEEMDNDIPFLLSDQKRILEF
SFKRNIIQNMNLAMLRDVTERKELEERLRKSDTLHVVGELAAGIAHEIRNPMTALKGFIQLLKGSIQNEDHALYFNVITS
ELKRIESIITEFLILAKPQAIMYEEKNIVKIMQDTMDLLHAQANLDNVQMHLKCEDGIPLIYCEPNQLKQVFINILKNAI
EVMPDGGNVYVSIKPKGDDHIIVSLADEGIGMTEDKLKRLGEPFYTTKERGTGLGLMVSYKIIEEHKGKIEVESEEGRGT
TFHLTLPIRQQHEEEA

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=368, Percent_Identity=27.9891304347826, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1790436, Length=235, Percent_Identity=33.6170212765957, Blast_Score=156, Evalue=5e-39,
Organism=Escherichia coli, GI1790300, Length=345, Percent_Identity=25.5072463768116, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1788713, Length=389, Percent_Identity=25.706940874036, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1786600, Length=371, Percent_Identity=24.2587601078167, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI48994928, Length=249, Percent_Identity=24.8995983935743, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI145693157, Length=286, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87081816, Length=225, Percent_Identity=25.7777777777778, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI1786783, Length=225, Percent_Identity=30.2222222222222, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1790346, Length=218, Percent_Identity=24.7706422018349, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789149, Length=277, Percent_Identity=26.3537906137184, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788393, Length=227, Percent_Identity=21.1453744493392, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 85033; Mature: 85033

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEETLDLNESLNLQNQLNRLRKENAKLKADLYQYELLFNGTLDAIFILDKQMNIIKANDA
CCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHEEEEEEECCCEEEEEEEECCCCEEECCHH
ACKILQSQKKNLLNRSALDFLHAVPPDELNQNIHRFLERGYAKKEVAIRLDNGSVKYIEF
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEE
TASKGIGEDCFFVVMRDISSKKILERERTINEKLFKDLFHRAVEGIVIFDKQGCFIDANH
ECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEECCC
SFCQSFEIEKSELSKLKLSQFVSPENKGRLDKIWNALFRRGKAKGELPVRLRSGEDRLFE
HHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCEEEE
LTITSNILNGFYMSIMRDITEKRSMEKQLFKSEERFREVFENAMDAIILMNNDGRIVKAN
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEECC
QSACKIFELPMDQLLNKKMTDFIDRTDQRYHAIQKQYDQKGEIRAELLFRMANGQYKELE
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEE
FTSKRIMFDSQDLTILRNVSDRKRMEQELRESELKFRKVFNGSMDGIVLFNNQYDIIDAN
EHHHHEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEECC
PLAGKILSIPLEQLKTSNLLDVISGYHIENAASPAKTISFEEMDNDIPFLLSDQKRILEF
CCCCHHHHCCHHHHCCCCHHHHHCCCCCCCCCCCHHEEEHHHCCCCCCEEECCCHHHHHH
SFKRNIIQNMNLAMLRDVTERKELEERLRKSDTLHVVGELAAGIAHEIRNPMTALKGFIQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHH
LLKGSIQNEDHALYFNVITSELKRIESIITEFLILAKPQAIMYEEKNIVKIMQDTMDLLH
HHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCHHHHHHHHHHHHHH
AQANLDNVQMHLKCEDGIPLIYCEPNQLKQVFINILKNAIEVMPDGGNVYVSIKPKGDDH
HHCCCCCEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCE
IIVSLADEGIGMTEDKLKRLGEPFYTTKERGTGLGLMVSYKIIEEHKGKIEVESEEGRGT
EEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEEEHHHCCCCEEEECCCCCCE
TFHLTLPIRQQHEEEA
EEEEEECCHHCCCCCC
>Mature Secondary Structure
MEETLDLNESLNLQNQLNRLRKENAKLKADLYQYELLFNGTLDAIFILDKQMNIIKANDA
CCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHEEEEEEECCCEEEEEEEECCCCEEECCHH
ACKILQSQKKNLLNRSALDFLHAVPPDELNQNIHRFLERGYAKKEVAIRLDNGSVKYIEF
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEE
TASKGIGEDCFFVVMRDISSKKILERERTINEKLFKDLFHRAVEGIVIFDKQGCFIDANH
ECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEECCC
SFCQSFEIEKSELSKLKLSQFVSPENKGRLDKIWNALFRRGKAKGELPVRLRSGEDRLFE
HHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCEEEE
LTITSNILNGFYMSIMRDITEKRSMEKQLFKSEERFREVFENAMDAIILMNNDGRIVKAN
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEECC
QSACKIFELPMDQLLNKKMTDFIDRTDQRYHAIQKQYDQKGEIRAELLFRMANGQYKELE
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEE
FTSKRIMFDSQDLTILRNVSDRKRMEQELRESELKFRKVFNGSMDGIVLFNNQYDIIDAN
EHHHHEEECCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEECC
PLAGKILSIPLEQLKTSNLLDVISGYHIENAASPAKTISFEEMDNDIPFLLSDQKRILEF
CCCCHHHHCCHHHHCCCCHHHHHCCCCCCCCCCCHHEEEHHHCCCCCCEEECCCHHHHHH
SFKRNIIQNMNLAMLRDVTERKELEERLRKSDTLHVVGELAAGIAHEIRNPMTALKGFIQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHH
LLKGSIQNEDHALYFNVITSELKRIESIITEFLILAKPQAIMYEEKNIVKIMQDTMDLLH
HHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCHHHHHHHHHHHHHH
AQANLDNVQMHLKCEDGIPLIYCEPNQLKQVFINILKNAIEVMPDGGNVYVSIKPKGDDH
HHCCCCCEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCE
IIVSLADEGIGMTEDKLKRLGEPFYTTKERGTGLGLMVSYKIIEEHKGKIEVESEEGRGT
EEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEEEEHHHCCCCEEEECCCCCCE
TFHLTLPIRQQHEEEA
EEEEEECCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]