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The map label for this gene is ogt [H]

Identifier: 52785275

GI number: 52785275

Start: 1484306

End: 1484803

Strand: Direct

Name: ogt [H]

Synonym: BLi01510

Alternate gene names: 52785275

Gene position: 1484306-1484803 (Clockwise)

Preceding gene: 52785274

Following gene: 52785279

Centisome position: 35.15

GC content: 49.0

Gene sequence:

>498_bases
GTGGGCTTTTATATTGAAATGACCTCGCCTTTTGGACAAGCCTACATCGTCGAGGAAAATAACCATATTACACATTTGCT
ATTTGAAAAAGAAGAACTAAAACAGCTTCAACAAGACGGCCTGGAGCTTCGCCCGGCCGAAACGGAATTGTTGAAGGAGG
CAAAGCGCCAGCTCGAAGAATATTTCCAGGGGGAGCGGAAAACGTTCACCCTTCCTCTCCGCCAGGATGGAACCCCGTTT
CAGAAAAAGGTGTGGGAGGCGCTGCAGTCGATCCCGTACGGTGAATCACGGAGCTATTTAGACATCGCATGCGCCGTCGG
GAATCCGAAAGCGGTGCGCGCAATCGGCCAAGCCAATAAAAGAAATGCGCTGCCGATTTTGATCCCGTGCCACCGTGTCA
TCGGCAAAAATCGTTCATTGACGGGGTATTCCGGAAATGAAACGGATAAAAAAGCGGTGCTGCTCGACATCGAAAATATT
GCGTATAAAGGAAAATAA

Upstream 100 bases:

>100_bases
AAAGGAAAAATCGAAGTTGAAAGTGAAGAAGGGAGAGGCACGACATTCCACCTGACTCTCCCGATCAGACAGCAGCATGA
GGAGGAGGCTTAATCGCATC

Downstream 100 bases:

>100_bases
TATACGAGCACGCCGTTTCTTTTTTTCGTCCGCGACATATAATTTTATTAAGTTAAAAATGTTGGCCTTGGTAAAAAAGG
AGGACGAATAAGTGAAAACA

Product: Ogt

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MGFYIEMTSPFGQAYIVEENNHITHLLFEKEELKQLQQDGLELRPAETELLKEAKRQLEEYFQGERKTFTLPLRQDGTPF
QKKVWEALQSIPYGESRSYLDIACAVGNPKAVRAIGQANKRNALPILIPCHRVIGKNRSLTGYSGNETDKKAVLLDIENI
AYKGK

Sequences:

>Translated_165_residues
MGFYIEMTSPFGQAYIVEENNHITHLLFEKEELKQLQQDGLELRPAETELLKEAKRQLEEYFQGERKTFTLPLRQDGTPF
QKKVWEALQSIPYGESRSYLDIACAVGNPKAVRAIGQANKRNALPILIPCHRVIGKNRSLTGYSGNETDKKAVLLDIENI
AYKGK
>Mature_164_residues
GFYIEMTSPFGQAYIVEENNHITHLLFEKEELKQLQQDGLELRPAETELLKEAKRQLEEYFQGERKTFTLPLRQDGTPFQ
KKVWEALQSIPYGESRSYLDIACAVGNPKAVRAIGQANKRNALPILIPCHRVIGKNRSLTGYSGNETDKKAVLLDIENIA
YKGK

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=113, Percent_Identity=35.3982300884956, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1787596, Length=99, Percent_Identity=45.4545454545455, Blast_Score=97, Evalue=5e-22,
Organism=Escherichia coli, GI1788542, Length=150, Percent_Identity=36, Blast_Score=92, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115533068, Length=77, Percent_Identity=49.3506493506493, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=77, Percent_Identity=49.3506493506493, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=123, Percent_Identity=37.3983739837398, Blast_Score=81, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18797; Mature: 18666

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFYIEMTSPFGQAYIVEENNHITHLLFEKEELKQLQQDGLELRPAETELLKEAKRQLEE
CCEEEEECCCCCCEEEEECCCCEEEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
YFQGERKTFTLPLRQDGTPFQKKVWEALQSIPYGESRSYLDIACAVGNPKAVRAIGQANK
HHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHCCCC
RNALPILIPCHRVIGKNRSLTGYSGNETDKKAVLLDIENIAYKGK
CCCCEEEEECHHHHCCCCCCCCCCCCCCCCEEEEEEEHHHEECCC
>Mature Secondary Structure 
GFYIEMTSPFGQAYIVEENNHITHLLFEKEELKQLQQDGLELRPAETELLKEAKRQLEE
CEEEEECCCCCCEEEEECCCCEEEEEECHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
YFQGERKTFTLPLRQDGTPFQKKVWEALQSIPYGESRSYLDIACAVGNPKAVRAIGQANK
HHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHCCCC
RNALPILIPCHRVIGKNRSLTGYSGNETDKKAVLLDIENIAYKGK
CCCCEEEEECHHHHCCCCCCCCCCCCCCCCEEEEEEEHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2505068; 2506524; 9384377 [H]