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The map label for this gene is proA

Identifier: 52785181

GI number: 52785181

Start: 1409725

End: 1410972

Strand: Direct

Name: proA

Synonym: BLi01413

Alternate gene names: 52785181

Gene position: 1409725-1410972 (Clockwise)

Preceding gene: 52785180

Following gene: 52785182

Centisome position: 33.38

GC content: 51.68

Gene sequence:

>1248_bases
ATGAGCGAAGTGATTGAAAAAGCAAAAAAGGCCAAAGTCGCAAAGGAAGAACTGGTTCATCAGCCGACTGAAAGGAAAAA
TGAGGCGCTTTCCTTTATCGCCGAGGCCATCCGCTTTAAGCAGGATGAGATATTGGCAGAAAACGAGAAAGATATCGTCC
GCGGCAAAGAAAAAGGCTTTTCGCCTGCGCTGCTTGACCGCCTTGCCTTAACGCCGGAGAGGCTGAATGATATAGCAGAC
GCCGTCATGCTGCTCACAAAGCTTGAAGACCCTGTGGGGGAAACGCTTGAAACGATCCGCAAAGACAACGGGCTTTTCAT
TGAAAATGTCAGGGTCCCGCTCGGAGTCGTCGGAATGATCTATGAGGCTCGTCCGAATGTAACGGTCGACGCCGCAACCC
TTTGTTTGAAAACGGGAAATGCCGTCATCCTGCGGGGCAGTTCGTCAGCCATCAACAGCAATAAAGCGCTCGTCCGCGTG
ATCAGGGAAGCGCTCGAACGCTCGGCGCTTCCAGAGGATGCCGTTCAGCTCATTGAAGATACAAGCAAAGAAACGGCAAA
ACAGCTGTTCACTTTAAACGACGGCTTGGATGTGCTCATCCCGAGAGGAGGGAAAAACCTCATCGATATGGTGGTGCGGG
AATCCACAGTGCCTGTGCTTGAAACAGGCGCGGGGAACTGCCACATATTCATTGATGAATCGGCTCAGCCAGACATGGCT
GAGCAGGTTGTAATCAATGCCAAAACGCAGCGCCCTTCTGTCTGCAATGCGATTGAAACGGTGCTCATCCATAAAGGCTG
GGCGGAAGAGCATACGAAAGCGCTTCTTCAAAAGCTTGAAGAAGCGGGCGTTGAAATCAGGGGAGACGAAGCCGTATGCA
CGATGCTGCCTTCTGCGGTTCCAGCTCGCGAGACCGACTGGGGAACCGAGTTTCTTGCGCCGGTTGTCAGCATCAAAACC
GTTGCCGGAATCGATGAAGCCATCCGGCACATCAGACAGTACGGCACACGTCATTCGGAAGCGATTCTGACTGAAAATCA
GGAAAACGCCCGGTATTTCTTAACGTCTGTTGACGCTGCAGCTGTTTACCACAATGCTTCAACACGTTTTACAGACGGCT
TTGAATTCGGCTACGGCGCCGAAATCGGCATCAGCACGCAAAAGCTTCACGCACGGGGGCCGATGGGCCTTAAAGCGCTG
ACTTCTTCTAAATATATCATTAAAGGAAACGGACAAATCCGGATATGA

Upstream 100 bases:

>100_bases
GGTAAAAGGGAAGCGAAGCGATGAGTTCCATCATGAAGAAGGGATAGAAGTCATCCATCGAAATGATTGGGTAAGCATTA
AAGAATAAGGGGATGAAAGA

Downstream 100 bases:

>100_bases
AAGTGTGGCATCCTGTCTCGAATGAAGACAGGATGTTTTTTGTTGTGTTATCACTTTTAATTTTATAAAATTAAATTGTT
CACAATATAAATTAAAGGAG

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR 1; Glutamate-5-semialdehyde dehydrogenase 1; Glutamyl-gamma-semialdehyde dehydrogenase 1; GSA dehydrogenase 1

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MSEVIEKAKKAKVAKEELVHQPTERKNEALSFIAEAIRFKQDEILAENEKDIVRGKEKGFSPALLDRLALTPERLNDIAD
AVMLLTKLEDPVGETLETIRKDNGLFIENVRVPLGVVGMIYEARPNVTVDAATLCLKTGNAVILRGSSSAINSNKALVRV
IREALERSALPEDAVQLIEDTSKETAKQLFTLNDGLDVLIPRGGKNLIDMVVRESTVPVLETGAGNCHIFIDESAQPDMA
EQVVINAKTQRPSVCNAIETVLIHKGWAEEHTKALLQKLEEAGVEIRGDEAVCTMLPSAVPARETDWGTEFLAPVVSIKT
VAGIDEAIRHIRQYGTRHSEAILTENQENARYFLTSVDAAAVYHNASTRFTDGFEFGYGAEIGISTQKLHARGPMGLKAL
TSSKYIIKGNGQIRI

Sequences:

>Translated_415_residues
MSEVIEKAKKAKVAKEELVHQPTERKNEALSFIAEAIRFKQDEILAENEKDIVRGKEKGFSPALLDRLALTPERLNDIAD
AVMLLTKLEDPVGETLETIRKDNGLFIENVRVPLGVVGMIYEARPNVTVDAATLCLKTGNAVILRGSSSAINSNKALVRV
IREALERSALPEDAVQLIEDTSKETAKQLFTLNDGLDVLIPRGGKNLIDMVVRESTVPVLETGAGNCHIFIDESAQPDMA
EQVVINAKTQRPSVCNAIETVLIHKGWAEEHTKALLQKLEEAGVEIRGDEAVCTMLPSAVPARETDWGTEFLAPVVSIKT
VAGIDEAIRHIRQYGTRHSEAILTENQENARYFLTSVDAAAVYHNASTRFTDGFEFGYGAEIGISTQKLHARGPMGLKAL
TSSKYIIKGNGQIRI
>Mature_414_residues
SEVIEKAKKAKVAKEELVHQPTERKNEALSFIAEAIRFKQDEILAENEKDIVRGKEKGFSPALLDRLALTPERLNDIADA
VMLLTKLEDPVGETLETIRKDNGLFIENVRVPLGVVGMIYEARPNVTVDAATLCLKTGNAVILRGSSSAINSNKALVRVI
REALERSALPEDAVQLIEDTSKETAKQLFTLNDGLDVLIPRGGKNLIDMVVRESTVPVLETGAGNCHIFIDESAQPDMAE
QVVINAKTQRPSVCNAIETVLIHKGWAEEHTKALLQKLEEAGVEIRGDEAVCTMLPSAVPARETDWGTEFLAPVVSIKTV
AGIDEAIRHIRQYGTRHSEAILTENQENARYFLTSVDAAAVYHNASTRFTDGFEFGYGAEIGISTQKLHARGPMGLKALT
SSKYIIKGNGQIRI

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI21361368, Length=396, Percent_Identity=34.8484848484849, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI62912457, Length=396, Percent_Identity=34.8484848484849, Blast_Score=242, Evalue=4e-64,
Organism=Escherichia coli, GI1786438, Length=408, Percent_Identity=47.5490196078431, Blast_Score=369, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17569967, Length=412, Percent_Identity=35.9223300970874, Blast_Score=268, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI17569969, Length=412, Percent_Identity=35.9223300970874, Blast_Score=268, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6324899, Length=438, Percent_Identity=34.2465753424658, Blast_Score=252, Evalue=6e-68,
Organism=Drosophila melanogaster, GI21357643, Length=407, Percent_Identity=35.3808353808354, Blast_Score=249, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA1_BACLD (Q65KU7)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_078602.1
- RefSeq:   YP_091010.1
- ProteinModelPortal:   Q65KU7
- SMR:   Q65KU7
- STRING:   Q65KU7
- EnsemblBacteria:   EBBACT00000058044
- EnsemblBacteria:   EBBACT00000060937
- GeneID:   3030461
- GeneID:   3097972
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01413
- KEGG:   bli:BL03751
- NMPDR:   fig|279010.5.peg.867
- eggNOG:   COG0014
- GeneTree:   EBGT00050000000020
- HOGENOM:   HBG318080
- OMA:   QYPAACN
- ProtClustDB:   PRK00197
- BioCyc:   BLIC279010-1:BLI01413-MONOMER
- BioCyc:   BLIC279010:BL03751-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PANTHER:   PTHR11063:SF1
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 45474; Mature: 45342

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVIEKAKKAKVAKEELVHQPTERKNEALSFIAEAIRFKQDEILAENEKDIVRGKEKGF
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHCCHHCCC
SPALLDRLALTPERLNDIADAVMLLTKLEDPVGETLETIRKDNGLFIENVRVPLGVVGMI
CHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
YEARPNVTVDAATLCLKTGNAVILRGSSSAINSNKALVRVIREALERSALPEDAVQLIED
EECCCCCEEHHHHHEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
TSKETAKQLFTLNDGLDVLIPRGGKNLIDMVVRESTVPVLETGAGNCHIFIDESAQPDMA
CCHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHCCCCEEECCCCCEEEEECCCCCCCHH
EQVVINAKTQRPSVCNAIETVLIHKGWAEEHTKALLQKLEEAGVEIRGDEAVCTMLPSAV
HHHEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCHHHHHCCCCC
PARETDWGTEFLAPVVSIKTVAGIDEAIRHIRQYGTRHSEAILTENQENARYFLTSVDAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEECHHH
AVYHNASTRFTDGFEFGYGAEIGISTQKLHARGPMGLKALTSSKYIIKGNGQIRI
HHEECCCCCCCCCCCCCCCCEECCCHHHHHCCCCCCHHHHCCCCEEEECCCEEEC
>Mature Secondary Structure 
SEVIEKAKKAKVAKEELVHQPTERKNEALSFIAEAIRFKQDEILAENEKDIVRGKEKGF
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHCCHHCCC
SPALLDRLALTPERLNDIADAVMLLTKLEDPVGETLETIRKDNGLFIENVRVPLGVVGMI
CHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
YEARPNVTVDAATLCLKTGNAVILRGSSSAINSNKALVRVIREALERSALPEDAVQLIED
EECCCCCEEHHHHHEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
TSKETAKQLFTLNDGLDVLIPRGGKNLIDMVVRESTVPVLETGAGNCHIFIDESAQPDMA
CCHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHCCCCEEECCCCCEEEEECCCCCCCHH
EQVVINAKTQRPSVCNAIETVLIHKGWAEEHTKALLQKLEEAGVEIRGDEAVCTMLPSAV
HHHEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCHHHHHCCCCC
PARETDWGTEFLAPVVSIKTVAGIDEAIRHIRQYGTRHSEAILTENQENARYFLTSVDAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEECHHH
AVYHNASTRFTDGFEFGYGAEIGISTQKLHARGPMGLKALTSSKYIIKGNGQIRI
HHEECCCCCCCCCCCCCCCCEECCCHHHHHCCCCCCHHHHCCCCEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA