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The map label for this gene is proB

Identifier: 52785180

GI number: 52785180

Start: 1408615

End: 1409712

Strand: Direct

Name: proB

Synonym: BLi01412

Alternate gene names: 52785180

Gene position: 1408615-1409712 (Clockwise)

Preceding gene: 52785179

Following gene: 52785181

Centisome position: 33.36

GC content: 50.09

Gene sequence:

>1098_bases
GTGAAAAAACAACGGATTGTCATTAAAATCGGAAGCAGTTCATTAACCAACTCGAAGGGCAGCATCGATGAGGAGAAGAT
CAATGACCATGTTCGGGCGATAGCTGCTCTGAAAAAAGAAGGCCATGAAGTCATCTTCATTTCGTCGGGGGCTGTCGCTG
CGGGATTTTTACAGCTCGGCTATCCCGCAAGGCCTGTGACGCTAAAGGGAAAACAGGCTGCAGCGGCCGTCGGCCAGAGC
TTGTTAATGCAAACATACATCGAGCATTTTGCAGACCACGATATCAAGCCTGCGCAGATCTTATTGACGAGAAACGATTT
TGCCAAACGGGAGCGCTATCGAAATGCATATGCGACCGTTATGGAACTGATTGAACGGGGGCTTGTCCCGATTATTAATG
AAAATGATTCGGTATCTGTTGAAGAATTGACGTTTGGGGACAACGACATGCTTTCCGCCCTTGTAAGCGGACTGATTCAT
GCCGACAAACTCATCATTTTAACGGATATTAACGGCCTCTATGACTCGAATCCGGCTGAACATCCCGATGCCCGCCGTTT
TGATTACATTCCCGAAATTACGGACGAACTTTTGGGCTGCGCCGCTTCCGCCGGATCAAAAGTCGGGACGGGCGGGATGA
AGTCGAAGCTTTTGGCGGCAAAAACCGCTCTTTCACTCGGCGTCAATGTTTTCATAGGCGCCGGAGAGGGAGATGATAAG
CTGATCCAGATTTTAAAAGGAAACGGGGACGGCACCTATATCGGCCAATCCGACCTGTCTTCCGTCAACAATCATAGGCA
GTGGATCGCCTTTCATTCACCTGTTTCAGGGAAGATTACCGTTGATGAAGGGGCTGAGCTGGCGATTACCGAAAACGGCG
GGAGCCTGCTGCCGGCCGGCGTAACAGCGATATCCGGAGATTTTCCGAAAGGGGCGGTTGTCGAAGTTTACGGACCGAAC
GGCTTGGCCGGGAAAGGCCAAACGCTGTACTCCGCCGCAGAGCTTGAAGAGGTAAAAGGGAAGCGAAGCGATGAGTTCCA
TCATGAAGAAGGGATAGAAGTCATCCATCGAAATGATTGGGTAAGCATTAAAGAATAA

Upstream 100 bases:

>100_bases
TGAAGGTGGTACCGCGGAAAGCTCTTTTCGTCCTTTTTTACTTGGACGAAAAGGGCTTTTTTTATTGGCCTTCTGCCAGA
TCATCTCGGAGGGAAAAAAC

Downstream 100 bases:

>100_bases
GGGGATGAAAGAATGAGCGAAGTGATTGAAAAAGCAAAAAAGGCCAAAGTCGCAAAGGAAGAACTGGTTCATCAGCCGAC
TGAAAGGAAAAATGAGGCGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase 1; GK 1

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKKQRIVIKIGSSSLTNSKGSIDEEKINDHVRAIAALKKEGHEVIFISSGAVAAGFLQLGYPARPVTLKGKQAAAAVGQS
LLMQTYIEHFADHDIKPAQILLTRNDFAKRERYRNAYATVMELIERGLVPIINENDSVSVEELTFGDNDMLSALVSGLIH
ADKLIILTDINGLYDSNPAEHPDARRFDYIPEITDELLGCAASAGSKVGTGGMKSKLLAAKTALSLGVNVFIGAGEGDDK
LIQILKGNGDGTYIGQSDLSSVNNHRQWIAFHSPVSGKITVDEGAELAITENGGSLLPAGVTAISGDFPKGAVVEVYGPN
GLAGKGQTLYSAAELEEVKGKRSDEFHHEEGIEVIHRNDWVSIKE

Sequences:

>Translated_365_residues
MKKQRIVIKIGSSSLTNSKGSIDEEKINDHVRAIAALKKEGHEVIFISSGAVAAGFLQLGYPARPVTLKGKQAAAAVGQS
LLMQTYIEHFADHDIKPAQILLTRNDFAKRERYRNAYATVMELIERGLVPIINENDSVSVEELTFGDNDMLSALVSGLIH
ADKLIILTDINGLYDSNPAEHPDARRFDYIPEITDELLGCAASAGSKVGTGGMKSKLLAAKTALSLGVNVFIGAGEGDDK
LIQILKGNGDGTYIGQSDLSSVNNHRQWIAFHSPVSGKITVDEGAELAITENGGSLLPAGVTAISGDFPKGAVVEVYGPN
GLAGKGQTLYSAAELEEVKGKRSDEFHHEEGIEVIHRNDWVSIKE
>Mature_365_residues
MKKQRIVIKIGSSSLTNSKGSIDEEKINDHVRAIAALKKEGHEVIFISSGAVAAGFLQLGYPARPVTLKGKQAAAAVGQS
LLMQTYIEHFADHDIKPAQILLTRNDFAKRERYRNAYATVMELIERGLVPIINENDSVSVEELTFGDNDMLSALVSGLIH
ADKLIILTDINGLYDSNPAEHPDARRFDYIPEITDELLGCAASAGSKVGTGGMKSKLLAAKTALSLGVNVFIGAGEGDDK
LIQILKGNGDGTYIGQSDLSSVNNHRQWIAFHSPVSGKITVDEGAELAITENGGSLLPAGVTAISGDFPKGAVVEVYGPN
GLAGKGQTLYSAAELEEVKGKRSDEFHHEEGIEVIHRNDWVSIKE

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=289, Percent_Identity=30.7958477508651, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI62912457, Length=287, Percent_Identity=30.6620209059233, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI1786437, Length=364, Percent_Identity=39.010989010989, Blast_Score=249, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17569969, Length=272, Percent_Identity=33.0882352941176, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17569967, Length=272, Percent_Identity=33.0882352941176, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6320506, Length=416, Percent_Identity=35.8173076923077, Blast_Score=214, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6321822, Length=408, Percent_Identity=32.5980392156863, Blast_Score=196, Evalue=5e-51,
Organism=Drosophila melanogaster, GI21357643, Length=290, Percent_Identity=33.7931034482759, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB1_BACLD (Q65KU8)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_078601.1
- RefSeq:   YP_091009.1
- ProteinModelPortal:   Q65KU8
- SMR:   Q65KU8
- STRING:   Q65KU8
- EnsemblBacteria:   EBBACT00000055891
- EnsemblBacteria:   EBBACT00000061581
- GeneID:   3030459
- GeneID:   3098709
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01412
- KEGG:   bli:BL03752
- NMPDR:   fig|279010.5.peg.866
- eggNOG:   COG0263
- GeneTree:   EBGT00050000001887
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   BLIC279010-1:BLI01412-MONOMER
- BioCyc:   BLIC279010:BL03752-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39115; Mature: 39115

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQRIVIKIGSSSLTNSKGSIDEEKINDHVRAIAALKKEGHEVIFISSGAVAAGFLQLG
CCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCC
YPARPVTLKGKQAAAAVGQSLLMQTYIEHFADHDIKPAQILLTRNDFAKRERYRNAYATV
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHH
MELIERGLVPIINENDSVSVEELTFGDNDMLSALVSGLIHADKLIILTDINGLYDSNPAE
HHHHHCCCCEEECCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
HPDARRFDYIPEITDELLGCAASAGSKVGTGGMKSKLLAAKTALSLGVNVFIGAGEGDDK
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCC
LIQILKGNGDGTYIGQSDLSSVNNHRQWIAFHSPVSGKITVDEGAELAITENGGSLLPAG
EEEEEECCCCCEEECHHHHHHHCCCCEEEEEECCCCCEEEECCCCEEEEECCCCCCCCCC
VTAISGDFPKGAVVEVYGPNGLAGKGQTLYSAAELEEVKGKRSDEFHHEEGIEVIHRNDW
HHEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHCCCEEEECCCE
VSIKE
EEECC
>Mature Secondary Structure
MKKQRIVIKIGSSSLTNSKGSIDEEKINDHVRAIAALKKEGHEVIFISSGAVAAGFLQLG
CCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCC
YPARPVTLKGKQAAAAVGQSLLMQTYIEHFADHDIKPAQILLTRNDFAKRERYRNAYATV
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHH
MELIERGLVPIINENDSVSVEELTFGDNDMLSALVSGLIHADKLIILTDINGLYDSNPAE
HHHHHCCCCEEECCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
HPDARRFDYIPEITDELLGCAASAGSKVGTGGMKSKLLAAKTALSLGVNVFIGAGEGDDK
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCC
LIQILKGNGDGTYIGQSDLSSVNNHRQWIAFHSPVSGKITVDEGAELAITENGGSLLPAG
EEEEEECCCCCEEECHHHHHHHCCCCEEEEEECCCCCEEEECCCCEEEEECCCCCCCCCC
VTAISGDFPKGAVVEVYGPNGLAGKGQTLYSAAELEEVKGKRSDEFHHEEGIEVIHRNDW
HHEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHCCCEEEECCCE
VSIKE
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA