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The map label for this gene is purU [H]

Identifier: 52785179

GI number: 52785179

Start: 1407374

End: 1408276

Strand: Direct

Name: purU [H]

Synonym: BLi01411

Alternate gene names: 52785179

Gene position: 1407374-1408276 (Clockwise)

Preceding gene: 52785178

Following gene: 52785180

Centisome position: 33.33

GC content: 46.4

Gene sequence:

>903_bases
ATGAACGCATATATGAAACAGCAGTTATCGCAATATCAGGAAATCAATAACGATAAGGCCCGCCTTCTGGTCAGCTGTCC
GGATCAGCCTGGAATCGTCGCCGCCGTTTCTTCATTTTTATTTGAGAACGGTGCGAATATTATTGAATCGAACCAGTATA
CGACAGACCCTGAAGGAGGCCGGTTCTTTCTGCGGATTGAGTTTGAAGTTCAAGGCATCCGCGAAAAGATCGAACAGATG
AAAGCGGCATTCGCCTCTGTGGCGGAGTCCTTCCAAATGACGTGGAGCATGAATTTGGCGAGCGAACTGAAACGCGTCGC
TATCTTCGTTTCAAAAGAGCTTCACTGCCTGCATGAACTGCTATGGGAATGGCAGAGCGGCAATTTGATGGCGGAGATTG
CAGCGGTTATCAGCAATCATGAAGATGCAAGAGAAACTGTTGAATCCCTGAATATTCCGTTCCTTTACATGAAAGCGAAC
AAAGACATTCGTCAGGAAGTGGAAAAACAGCAGCTGAAATGGCTTGAAGAGTATCGTGCGGATGTCATCGTTCTCGCCCG
ATATATGCAGATTTTAACGCCTGATTTTGTCTCGGCTCATCCGAACAAAATCATAAATATTCACCACTCCTTCCTGCCGG
CTTTTATCGGGGCGAATCCGTATAAACGGGCATATGAAAGAGGCGTTAAACTGATCGGAGCAACATCGCATTATGTGACA
AACGAATTGGATGAAGGGCCGATCATCGAACAGGATATCGAACGGGTCGACCACAGAGACAATGTGGAAGCATTGAAAAA
CATCGGCCGAACGATTGAGAGAAGCGTTTTGGCAAGAGCGGTCAAATGGCACTTGGAAGACCGGATTATCGTGCATGGAA
ACAAGACGATCGTCTTTAATTAA

Upstream 100 bases:

>100_bases
GGGTTGATGCAAATAGGCGTTCGTAAATTGATTTTTCAATAAACTCCAGTATAATGAAAAGATAGTTTACGAATTACAAG
CAAAGAGGGTTTTTACGAAA

Downstream 100 bases:

>100_bases
ATTGTTTGATCTGCCTCTTGACAGAGGCGTTTTTCCTAGGCATAATAATAAAAAATCACGAGCGTTGAAGAGGGCCAGTA
AGCAGTTCTTTCTGTGAAAG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MNAYMKQQLSQYQEINNDKARLLVSCPDQPGIVAAVSSFLFENGANIIESNQYTTDPEGGRFFLRIEFEVQGIREKIEQM
KAAFASVAESFQMTWSMNLASELKRVAIFVSKELHCLHELLWEWQSGNLMAEIAAVISNHEDARETVESLNIPFLYMKAN
KDIRQEVEKQQLKWLEEYRADVIVLARYMQILTPDFVSAHPNKIINIHHSFLPAFIGANPYKRAYERGVKLIGATSHYVT
NELDEGPIIEQDIERVDHRDNVEALKNIGRTIERSVLARAVKWHLEDRIIVHGNKTIVFN

Sequences:

>Translated_300_residues
MNAYMKQQLSQYQEINNDKARLLVSCPDQPGIVAAVSSFLFENGANIIESNQYTTDPEGGRFFLRIEFEVQGIREKIEQM
KAAFASVAESFQMTWSMNLASELKRVAIFVSKELHCLHELLWEWQSGNLMAEIAAVISNHEDARETVESLNIPFLYMKAN
KDIRQEVEKQQLKWLEEYRADVIVLARYMQILTPDFVSAHPNKIINIHHSFLPAFIGANPYKRAYERGVKLIGATSHYVT
NELDEGPIIEQDIERVDHRDNVEALKNIGRTIERSVLARAVKWHLEDRIIVHGNKTIVFN
>Mature_300_residues
MNAYMKQQLSQYQEINNDKARLLVSCPDQPGIVAAVSSFLFENGANIIESNQYTTDPEGGRFFLRIEFEVQGIREKIEQM
KAAFASVAESFQMTWSMNLASELKRVAIFVSKELHCLHELLWEWQSGNLMAEIAAVISNHEDARETVESLNIPFLYMKAN
KDIRQEVEKQQLKWLEEYRADVIVLARYMQILTPDFVSAHPNKIINIHHSFLPAFIGANPYKRAYERGVKLIGATSHYVT
NELDEGPIIEQDIERVDHRDNVEALKNIGRTIERSVLARAVKWHLEDRIIVHGNKTIVFN

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=219, Percent_Identity=29.2237442922374, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI209869995, Length=219, Percent_Identity=29.2237442922374, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI209869993, Length=219, Percent_Identity=29.2237442922374, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1787483, Length=274, Percent_Identity=44.8905109489051, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1788846, Length=192, Percent_Identity=28.6458333333333, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17567511, Length=164, Percent_Identity=32.3170731707317, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24582400, Length=202, Percent_Identity=29.2079207920792, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 34530; Mature: 34530

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAYMKQQLSQYQEINNDKARLLVSCPDQPGIVAAVSSFLFENGANIIESNQYTTDPEGG
CCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCC
RFFLRIEFEVQGIREKIEQMKAAFASVAESFQMTWSMNLASELKRVAIFVSKELHCLHEL
EEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHH
LWEWQSGNLMAEIAAVISNHEDARETVESLNIPFLYMKANKDIRQEVEKQQLKWLEEYRA
HHHCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
DVIVLARYMQILTPDFVSAHPNKIINIHHSFLPAFIGANPYKRAYERGVKLIGATSHYVT
HHHHHHHHHHHHCCHHHCCCCCCEEEEHHHHHHHHHCCCHHHHHHHCCCEEEECCHHHHH
NELDEGPIIEQDIERVDHRDNVEALKNIGRTIERSVLARAVKWHLEDRIIVHGNKTIVFN
CCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEC
>Mature Secondary Structure
MNAYMKQQLSQYQEINNDKARLLVSCPDQPGIVAAVSSFLFENGANIIESNQYTTDPEGG
CCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCC
RFFLRIEFEVQGIREKIEQMKAAFASVAESFQMTWSMNLASELKRVAIFVSKELHCLHEL
EEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHH
LWEWQSGNLMAEIAAVISNHEDARETVESLNIPFLYMKANKDIRQEVEKQQLKWLEEYRA
HHHCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
DVIVLARYMQILTPDFVSAHPNKIINIHHSFLPAFIGANPYKRAYERGVKLIGATSHYVT
HHHHHHHHHHHHCCHHHCCCCCCEEEEHHHHHHHHHCCCHHHHHHHCCCEEEECCHHHHH
NELDEGPIIEQDIERVDHRDNVEALKNIGRTIERSVLARAVKWHLEDRIIVHGNKTIVFN
CCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]