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The map label for this gene is thiF [H]

Identifier: 52785035

GI number: 52785035

Start: 1274743

End: 1275753

Strand: Direct

Name: thiF [H]

Synonym: BLi01264

Alternate gene names: 52785035

Gene position: 1274743-1275753 (Clockwise)

Preceding gene: 52785034

Following gene: 52785036

Centisome position: 30.19

GC content: 53.21

Gene sequence:

>1011_bases
TTGACCGGCAGGTATTCAAGACAGGAGCTCTTTCACCCGATCGGACCGGACGGCCAGGAAAAATTAAGCCGCGCCCGCGT
CCTCGTGATCGGTGCTGGGGCTTTGGGCGCGGCCAGCGCAGAAATGCTGGTGAGGGCCGGGGTCGGTTCGCTGACGATAG
CCGACAGAGATTATGTGGAATGGAGCAATCTCCAGCGCCAGCAGCTCTATACGGAACAGGATGTGATTGATCATATGCCA
AAGGCCGCCGCCGCTGAAAAAAGGCTGCGCCTGATCAACAGCTCTGTTCAAGTAACCGGAATCGTAGCGGATGTTACTGC
GGAAAAAGCGCTCGAGCTTGCGGAGGAAGCGTCCCTGATCGTCGATGCGACGGATAATTTTGAAACACGGCTGATCATGA
ACGACGCGGCGGTCAAATTAGGCATCCCTTTCCTGTACGGCGCGTGTGTCGCAAGCTATGGCATCACATATAGCGTAATT
CCGGGAAAAACGCCGTGTCTTCATTGTCTTTTGGGGCATTTGCCTGCGAATACCATGACGTGCGACACAGCAGGGGTGAT
CGGTCCGGTCGTCCAGCAGGTTGCCGCATACCAGGTAACAGATGCCCTGAAGCTGCTGACCGGGCATGAACCGTCAGGGA
TGCTCCGCTCATTTGATATTTGGACAAATGAGCGGTCTGAAGTCCGGGCTGACACGCTTAAACATGAGACATGTCCGACG
TGCGGAAAAGGCGAGTTTCCGTTTCTTTCAGCTGAAAACGAAACAAAAGCAGCCGTTCTTTGCGGCAGACAAACGGTTCA
GATCAGGCCGGCCGCGGAACAGCCGCTGGAACTGGAATCGCTTGCTGCCAAATTAAAAAAAGCCGGTTTAAATGTGACGG
GAAATCCTTATTTAGTTTCCTGCCGTGCGGAGGATTTTAAGTTCGTGATTTTTCGGGATGGACGTGCTTTAATTCACGGA
ACGAATGACATCAATCGCGCGAAAACGATATATCACAGGTGGATTGGCTAA

Upstream 100 bases:

>100_bases
AGTTTGCCGTTGAAGCGGGCAGGCTCGGATATCTTGCCGGACGAATTCCGGAAAAGAACTACGGCATCGCCAGCAGCCCT
GAAGAAGGGAAGTTGACGAT

Downstream 100 bases:

>100_bases
AAGGCCGAAGGGAGTGCAAACATCATGAGCATATATAAAGCATTAACAATCGCCGGATCGGATTCAGGAGGCGGAGCCGG
CATTCAAGCCGACATTAAAA

Product: thiamine/molybdopterin biosynthesis ThiF/MoeB-like protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTGRYSRQELFHPIGPDGQEKLSRARVLVIGAGALGAASAEMLVRAGVGSLTIADRDYVEWSNLQRQQLYTEQDVIDHMP
KAAAAEKRLRLINSSVQVTGIVADVTAEKALELAEEASLIVDATDNFETRLIMNDAAVKLGIPFLYGACVASYGITYSVI
PGKTPCLHCLLGHLPANTMTCDTAGVIGPVVQQVAAYQVTDALKLLTGHEPSGMLRSFDIWTNERSEVRADTLKHETCPT
CGKGEFPFLSAENETKAAVLCGRQTVQIRPAAEQPLELESLAAKLKKAGLNVTGNPYLVSCRAEDFKFVIFRDGRALIHG
TNDINRAKTIYHRWIG

Sequences:

>Translated_336_residues
MTGRYSRQELFHPIGPDGQEKLSRARVLVIGAGALGAASAEMLVRAGVGSLTIADRDYVEWSNLQRQQLYTEQDVIDHMP
KAAAAEKRLRLINSSVQVTGIVADVTAEKALELAEEASLIVDATDNFETRLIMNDAAVKLGIPFLYGACVASYGITYSVI
PGKTPCLHCLLGHLPANTMTCDTAGVIGPVVQQVAAYQVTDALKLLTGHEPSGMLRSFDIWTNERSEVRADTLKHETCPT
CGKGEFPFLSAENETKAAVLCGRQTVQIRPAAEQPLELESLAAKLKKAGLNVTGNPYLVSCRAEDFKFVIFRDGRALIHG
TNDINRAKTIYHRWIG
>Mature_335_residues
TGRYSRQELFHPIGPDGQEKLSRARVLVIGAGALGAASAEMLVRAGVGSLTIADRDYVEWSNLQRQQLYTEQDVIDHMPK
AAAAEKRLRLINSSVQVTGIVADVTAEKALELAEEASLIVDATDNFETRLIMNDAAVKLGIPFLYGACVASYGITYSVIP
GKTPCLHCLLGHLPANTMTCDTAGVIGPVVQQVAAYQVTDALKLLTGHEPSGMLRSFDIWTNERSEVRADTLKHETCPTC
GKGEFPFLSAENETKAAVLCGRQTVQIRPAAEQPLELESLAAKLKKAGLNVTGNPYLVSCRAEDFKFVIFRDGRALIHGT
NDINRAKTIYHRWIG

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=220, Percent_Identity=36.3636363636364, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI38045948, Length=141, Percent_Identity=34.7517730496454, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI87082356, Length=243, Percent_Identity=39.5061728395062, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1787048, Length=243, Percent_Identity=34.156378600823, Blast_Score=134, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17540406, Length=245, Percent_Identity=32.2448979591837, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI71984476, Length=174, Percent_Identity=28.735632183908, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6321903, Length=275, Percent_Identity=30.1818181818182, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6320598, Length=170, Percent_Identity=30.5882352941176, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6325323, Length=192, Percent_Identity=29.6875, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582879, Length=242, Percent_Identity=30.5785123966942, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 36538; Mature: 36407

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRYSRQELFHPIGPDGQEKLSRARVLVIGAGALGAASAEMLVRAGVGSLTIADRDYVE
CCCCCCHHHHCCCCCCCHHHHHHHCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCHH
WSNLQRQQLYTEQDVIDHMPKAAAAEKRLRLINSSVQVTGIVADVTAEKALELAEEASLI
HHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHCCEE
VDATDNFETRLIMNDAAVKLGIPFLYGACVASYGITYSVIPGKTPCLHCLLGHLPANTMT
EECCCCCCEEEEEECCEEEECCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCEE
CDTAGVIGPVVQQVAAYQVTDALKLLTGHEPSGMLRSFDIWTNERSEVRADTLKHETCPT
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCC
CGKGEFPFLSAENETKAAVLCGRQTVQIRPAAEQPLELESLAAKLKKAGLNVTGNPYLVS
CCCCCCCEEECCCCCCEEEEECCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEE
CRAEDFKFVIFRDGRALIHGTNDINRAKTIYHRWIG
EECCCEEEEEEECCEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TGRYSRQELFHPIGPDGQEKLSRARVLVIGAGALGAASAEMLVRAGVGSLTIADRDYVE
CCCCCHHHHCCCCCCCHHHHHHHCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCHH
WSNLQRQQLYTEQDVIDHMPKAAAAEKRLRLINSSVQVTGIVADVTAEKALELAEEASLI
HHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHHHCCEE
VDATDNFETRLIMNDAAVKLGIPFLYGACVASYGITYSVIPGKTPCLHCLLGHLPANTMT
EECCCCCCEEEEEECCEEEECCHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCEE
CDTAGVIGPVVQQVAAYQVTDALKLLTGHEPSGMLRSFDIWTNERSEVRADTLKHETCPT
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCC
CGKGEFPFLSAENETKAAVLCGRQTVQIRPAAEQPLELESLAAKLKKAGLNVTGNPYLVS
CCCCCCCEEECCCCCCEEEEECCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCEEEE
CRAEDFKFVIFRDGRALIHGTNDINRAKTIYHRWIG
EECCCEEEEEEECCEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]