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The map label for this gene is thiG

Identifier: 52785034

GI number: 52785034

Start: 1273979

End: 1274746

Strand: Direct

Name: thiG

Synonym: BLi01263

Alternate gene names: 52785034

Gene position: 1273979-1274746 (Clockwise)

Preceding gene: 52785033

Following gene: 52785035

Centisome position: 30.17

GC content: 51.3

Gene sequence:

>768_bases
ATGTTAAAAATCGCAGATCGTCAATTTTCATCAAGATTATTGCTCGGTACAGGGAAGTATCCGTCATTTGAGATTCAAAA
AGAGGCCGTCAGCGCATCTGAATCGGAGATCCTGACGTTTGCCGTCAGACGGATGAATATTTTTGAAGAATCTCAGCCTA
ATTTTTTGGAGCAGCTCGATTTAAGCCGTTATACGCTTTTGCCGAATACGGCAGGAGCAAAAACGGCTGAGGAAGCCGTC
CGGATCGCCAAGCTTGCCAAAGCAAGCGGTCTTTGCGACATGATCAAAGTCGAAGTGATCGGCTGTGACCGTTCGCTTCT
GCCGGACCCGGTAGAAACGCTGAAGGCGTCAGAAACCTTGCTTGAAGAAGGCTTCATTGTACTGCCGTATACATCGGATG
ATGTCGTCCTCGCGAGAAAACTTGAAGAGCTCGGCGTGCACGCCATTATGCCCGGGGCCTCTCCGATCGGTTCGGGACAA
GGAATCATCAATCCGCTCAACCTGTCGTTCATCATTGAACAGGCTAAGGTTCCCGTTATCGTGGATGCCGGAATCGGTTC
TCCGAAAGATGCGGCATACGCAATGGAACTCGGTGCTGACGGGGTGCTTTTGAACACGGCAGTGAGCGGGGCGAAAGATC
CGGTCCAAATGGCGAAGGCTATGAAGTTTGCCGTTGAAGCGGGCAGGCTCGGATATCTTGCCGGACGAATTCCGGAAAAG
AACTACGGCATCGCCAGCAGCCCTGAAGAAGGGAAGTTGACGATTTGA

Upstream 100 bases:

>100_bases
AAACAGGCGGATTATCGGAAAAGACCAATATCAAACGGTCAAATTGGAAGAACACGATGTCATCGAAATCGTTCATTTTG
TCGGAGGGGGATAAACCGTT

Downstream 100 bases:

>100_bases
CCGGCAGGTATTCAAGACAGGAGCTCTTTCACCCGATCGGACCGGACGGCCAGGAAAAATTAAGCCGCGCCCGCGTCCTC
GTGATCGGTGCTGGGGCTTT

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLKIADRQFSSRLLLGTGKYPSFEIQKEAVSASESEILTFAVRRMNIFEESQPNFLEQLDLSRYTLLPNTAGAKTAEEAV
RIAKLAKASGLCDMIKVEVIGCDRSLLPDPVETLKASETLLEEGFIVLPYTSDDVVLARKLEELGVHAIMPGASPIGSGQ
GIINPLNLSFIIEQAKVPVIVDAGIGSPKDAAYAMELGADGVLLNTAVSGAKDPVQMAKAMKFAVEAGRLGYLAGRIPEK
NYGIASSPEEGKLTI

Sequences:

>Translated_255_residues
MLKIADRQFSSRLLLGTGKYPSFEIQKEAVSASESEILTFAVRRMNIFEESQPNFLEQLDLSRYTLLPNTAGAKTAEEAV
RIAKLAKASGLCDMIKVEVIGCDRSLLPDPVETLKASETLLEEGFIVLPYTSDDVVLARKLEELGVHAIMPGASPIGSGQ
GIINPLNLSFIIEQAKVPVIVDAGIGSPKDAAYAMELGADGVLLNTAVSGAKDPVQMAKAMKFAVEAGRLGYLAGRIPEK
NYGIASSPEEGKLTI
>Mature_255_residues
MLKIADRQFSSRLLLGTGKYPSFEIQKEAVSASESEILTFAVRRMNIFEESQPNFLEQLDLSRYTLLPNTAGAKTAEEAV
RIAKLAKASGLCDMIKVEVIGCDRSLLPDPVETLKASETLLEEGFIVLPYTSDDVVLARKLEELGVHAIMPGASPIGSGQ
GIINPLNLSFIIEQAKVPVIVDAGIGSPKDAAYAMELGADGVLLNTAVSGAKDPVQMAKAMKFAVEAGRLGYLAGRIPEK
NYGIASSPEEGKLTI

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=253, Percent_Identity=49.802371541502, Blast_Score=236, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_BACLD (Q65L94)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_078463.1
- RefSeq:   YP_090863.1
- ProteinModelPortal:   Q65L94
- SMR:   Q65L94
- STRING:   Q65L94
- EnsemblBacteria:   EBBACT00000056061
- EnsemblBacteria:   EBBACT00000062007
- GeneID:   3030270
- GeneID:   3099871
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01263
- KEGG:   bli:BL01588
- NMPDR:   fig|279010.5.peg.2480
- eggNOG:   COG2022
- GeneTree:   EBGT00050000002572
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- BioCyc:   BLIC279010-1:BLI01263-MONOMER
- BioCyc:   BLIC279010:BL01588-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27253; Mature: 27253

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: ACT_SITE 96-96 BINDING 157-157

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIADRQFSSRLLLGTGKYPSFEIQKEAVSASESEILTFAVRRMNIFEESQPNFLEQLD
CCEECCCCCCCEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHC
LSRYTLLPNTAGAKTAEEAVRIAKLAKASGLCDMIKVEVIGCDRSLLPDPVETLKASETL
CCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHH
LEEGFIVLPYTSDDVVLARKLEELGVHAIMPGASPIGSGQGIINPLNLSFIIEQAKVPVI
HHCCEEEEEECCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCEE
VDAGIGSPKDAAYAMELGADGVLLNTAVSGAKDPVQMAKAMKFAVEAGRLGYLAGRIPEK
EECCCCCCCCCCEEEECCCCCEEEEEHHCCCCCHHHHHHHHHHHHHCCCEEEHCCCCCCC
NYGIASSPEEGKLTI
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MLKIADRQFSSRLLLGTGKYPSFEIQKEAVSASESEILTFAVRRMNIFEESQPNFLEQLD
CCEECCCCCCCEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHC
LSRYTLLPNTAGAKTAEEAVRIAKLAKASGLCDMIKVEVIGCDRSLLPDPVETLKASETL
CCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHH
LEEGFIVLPYTSDDVVLARKLEELGVHAIMPGASPIGSGQGIINPLNLSFIIEQAKVPVI
HHCCEEEEEECCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCEE
VDAGIGSPKDAAYAMELGADGVLLNTAVSGAKDPVQMAKAMKFAVEAGRLGYLAGRIPEK
EECCCCCCCCCCEEEECCCCCEEEEEHHCCCCCHHHHHHHHHHHHHCCCEEEHCCCCCCC
NYGIASSPEEGKLTI
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA