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The map label for this gene is trpS [H]

Identifier: 52785003

GI number: 52785003

Start: 1242505

End: 1243497

Strand: Reverse

Name: trpS [H]

Synonym: BLi01231

Alternate gene names: 52785003

Gene position: 1243497-1242505 (Counterclockwise)

Preceding gene: 52785015

Following gene: 52784992

Centisome position: 29.45

GC content: 49.24

Gene sequence:

>993_bases
ATGAAAAAAACGATTTTTTCCGGTATCCAGCCGAGCGGCTCTGTCACCCTCGGCAACTATATCGGAGCGATGAAGCAGTT
TGTCGACTTGCAGGATGACTACAACTGTTACTTCTGCATCGTCGATCAGCACGCGATCACAGTACCGCAGGACCGGCTCG
CGCTGAGAAAGAACATCCGGAACCTTGCCGCGCTGTATTTGGCGATCGGCCTCGATCCGAAAAAAGCGACGCTGTTTATC
CAATCGGAAGTGCCTGCACACGCCCAGGCGGGATGGATGCTTCAGTGTGTCGCCTACATCGGCGAGCTTGAGCGCATGAC
GCAGTACAAAGATAAATCAGCCGGAAAAGAAGCTGTCGTCTCCGGGCTTTTGACATATCCTCCATTAATGGCTGCGGACA
TTCTGCTTTATGGAACAGACGTCGTCCCTGTCGGAGAAGATCAGAAGCAGCATCTTGAATTAACCCGGAACTTGGCGGAG
CGTTTCAATAAGAAATATAATGACATTTTTACGGTTCCGGAGGTTAAAATACCGAAGGTCGGCGCCCGCATTATGTCACT
TGCCGACCCGCTCAAAAAGATGAGCAAATCAGATCCGAACCAAAAAGCGTTCATCACCCTGCTCGACGAACCAAAACAGC
TTGAGAAAAAAATCAAAAGCGCTGTCACGGATTCCGACGGAATCGTCAAATATGATAAAGAAAACAAACCCGGTGTATCC
AACCTTCTGACGATCTATTCGATTCTCGGACAAGCCTCAATCGAAGAGCTTGAAGCAAAATACGAAGGCAAAGGCTACGG
CGAATTTAAATCAGACCTTGCCGAAGTCGTCATCGGCGCGCTGAAACCGATTCAAGACCGTTACCACGAATTGATCGAAT
CTGAAGAGCTGGACCGGATTTTGGACGAAGGCGCCGAACGCGCAAATCAAACAGCAAACAAGATGCTAAAAAAAATGGAA
AACGCGATGGGACTCGGCCGAAAAAGACGCTGA

Upstream 100 bases:

>100_bases
ACCATAATGAAATAATCAGGGTGGCACCACGGTTCATTCGTCCCTTTTCTTTTAAGGGAAGAATGGGCCTTTTTATTTTG
AAATGGAAAGAGGGATTCAC

Downstream 100 bases:

>100_bases
TCCAAAAACACCTTCCGATTGTACGATAATCGGAAGGTGTTTTTCATAGAGAACAGCCGCAGCTCCGCATTCACTTGCCT
CCGGATCACGATATTTCTAT

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKKTIFSGIQPSGSVTLGNYIGAMKQFVDLQDDYNCYFCIVDQHAITVPQDRLALRKNIRNLAALYLAIGLDPKKATLFI
QSEVPAHAQAGWMLQCVAYIGELERMTQYKDKSAGKEAVVSGLLTYPPLMAADILLYGTDVVPVGEDQKQHLELTRNLAE
RFNKKYNDIFTVPEVKIPKVGARIMSLADPLKKMSKSDPNQKAFITLLDEPKQLEKKIKSAVTDSDGIVKYDKENKPGVS
NLLTIYSILGQASIEELEAKYEGKGYGEFKSDLAEVVIGALKPIQDRYHELIESEELDRILDEGAERANQTANKMLKKME
NAMGLGRKRR

Sequences:

>Translated_330_residues
MKKTIFSGIQPSGSVTLGNYIGAMKQFVDLQDDYNCYFCIVDQHAITVPQDRLALRKNIRNLAALYLAIGLDPKKATLFI
QSEVPAHAQAGWMLQCVAYIGELERMTQYKDKSAGKEAVVSGLLTYPPLMAADILLYGTDVVPVGEDQKQHLELTRNLAE
RFNKKYNDIFTVPEVKIPKVGARIMSLADPLKKMSKSDPNQKAFITLLDEPKQLEKKIKSAVTDSDGIVKYDKENKPGVS
NLLTIYSILGQASIEELEAKYEGKGYGEFKSDLAEVVIGALKPIQDRYHELIESEELDRILDEGAERANQTANKMLKKME
NAMGLGRKRR
>Mature_330_residues
MKKTIFSGIQPSGSVTLGNYIGAMKQFVDLQDDYNCYFCIVDQHAITVPQDRLALRKNIRNLAALYLAIGLDPKKATLFI
QSEVPAHAQAGWMLQCVAYIGELERMTQYKDKSAGKEAVVSGLLTYPPLMAADILLYGTDVVPVGEDQKQHLELTRNLAE
RFNKKYNDIFTVPEVKIPKVGARIMSLADPLKKMSKSDPNQKAFITLLDEPKQLEKKIKSAVTDSDGIVKYDKENKPGVS
NLLTIYSILGQASIEELEAKYEGKGYGEFKSDLAEVVIGALKPIQDRYHELIESEELDRILDEGAERANQTANKMLKKME
NAMGLGRKRR

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=327, Percent_Identity=40.9785932721713, Blast_Score=258, Evalue=5e-69,
Organism=Homo sapiens, GI41352700, Length=175, Percent_Identity=51.4285714285714, Blast_Score=185, Evalue=6e-47,
Organism=Escherichia coli, GI1789786, Length=328, Percent_Identity=56.7073170731707, Blast_Score=375, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71982800, Length=328, Percent_Identity=38.719512195122, Blast_Score=218, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI71982793, Length=328, Percent_Identity=38.719512195122, Blast_Score=217, Evalue=8e-57,
Organism=Saccharomyces cerevisiae, GI6320474, Length=344, Percent_Identity=41.8604651162791, Blast_Score=239, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24666151, Length=327, Percent_Identity=40.9785932721713, Blast_Score=249, Evalue=3e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 36905; Mature: 36905

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTIFSGIQPSGSVTLGNYIGAMKQFVDLQDDYNCYFCIVDQHAITVPQDRLALRKNIR
CCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCEEEEEEECCEEECCHHHHHHHHHHH
NLAALYLAIGLDPKKATLFIQSEVPAHAQAGWMLQCVAYIGELERMTQYKDKSAGKEAVV
HHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SGLLTYPPLMAADILLYGTDVVPVGEDQKQHLELTRNLAERFNKKYNDIFTVPEVKIPKV
HHHHHCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCHH
GARIMSLADPLKKMSKSDPNQKAFITLLDEPKQLEKKIKSAVTDSDGIVKYDKENKPGVS
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHH
NLLTIYSILGQASIEELEAKYEGKGYGEFKSDLAEVVIGALKPIQDRYHELIESEELDRI
HHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDEGAERANQTANKMLKKMENAMGLGRKRR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKTIFSGIQPSGSVTLGNYIGAMKQFVDLQDDYNCYFCIVDQHAITVPQDRLALRKNIR
CCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCEEEEEEECCEEECCHHHHHHHHHHH
NLAALYLAIGLDPKKATLFIQSEVPAHAQAGWMLQCVAYIGELERMTQYKDKSAGKEAVV
HHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SGLLTYPPLMAADILLYGTDVVPVGEDQKQHLELTRNLAERFNKKYNDIFTVPEVKIPKV
HHHHHCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCHH
GARIMSLADPLKKMSKSDPNQKAFITLLDEPKQLEKKIKSAVTDSDGIVKYDKENKPGVS
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHH
NLLTIYSILGQASIEELEAKYEGKGYGEFKSDLAEVVIGALKPIQDRYHELIESEELDRI
HHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDEGAERANQTANKMLKKMENAMGLGRKRR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3149612; 9384377; 2494170 [H]