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The map label for this gene is yfmE [H]

Identifier: 52784885

GI number: 52784885

Start: 1113581

End: 1114609

Strand: Direct

Name: yfmE [H]

Synonym: BLi01113

Alternate gene names: 52784885

Gene position: 1113581-1114609 (Clockwise)

Preceding gene: 52784884

Following gene: 52784889

Centisome position: 26.37

GC content: 53.94

Gene sequence:

>1029_bases
ATGAACAATCCGGCAAGAAAGACGAAAAGGCGGCGCGGACAATCGGCGGTTGTTCTCTGTGCGCTATTCGCCTGTTTGTT
TGTGCTCATTGTACTCAATTTGGCGATCGGCGATGAAAACATCGGACTGTGGGACGTATTGAAATCGCTGGCAGGACAAG
GCGATGCGGGAGTCCGCTTTTTGGTGATGGAATTTCGAATGCCGCGAATCCTCCTCGCTGTGCTTGCCGGAGGGGCGCTT
TCGATCGCGGGTGTAGTCGCTCAATTTCTGATGAAAAATCCGCTGGCCGCCCCTGACACGCTTGGGATTACAGGAGGAGC
GGGATTGGGCGCTGTGATCGTTACGGTCTTGTTTTCACAGCAAAGTCCGCTTCTGCTGACAGGCGCCGCCTTTTCAGGAA
GCGTTTTAGCTGCGATCCTCGTCTATTTATTGGCTTATCGAAATGGGATCTCTCCTGTGCGGCTGGCTCTTGTCGGGATA
GCGGTCAATGCTTTCTGCCATTCGGGCATTCAGCTGTTAATCTCAAGGGGGAGCCCGAATGTCAACAGCGCATTAATTTG
GCTGAACGGAAGTCTGTGGGGAAGAGGCTGGCAGGATGTATTGCCTCTTTTCATATGGTGCTGTCTGGTGATTCCTCCGA
TATGGCTTGCGGCAAAACCGCTCGATATCATCAAATCAGGGGACGGGATTGCCGTCGGCCTCGGCATCCGGCTTGAAAGA
ACAAGGTTTCTGCTGCTTGCCGGTGCGGTTTTGCTGACAGCTGCTGCTGTGACCGCTGTGGGAACGATCGGTTTTATCGG
GCTGATGGCGCCTCATATCGCAAGAAAGCTCGCAGGGGCTGAAACGAAGATCTTGATTCCCGTTGCAGCTCTCGTCGGCG
GCATCGTCCTTTTGATAGCCGATGCCGCCGGAAGAGGTCTTGTTCCGCCGCTTGAAATACCGGCCGGATTGATCGCAGCC
GTTATCGGCGGGCCGTATTTTATCTATTTGCTGCGCCGCGAAGAACATAAAAAAAGACGGCATGCTTGA

Upstream 100 bases:

>100_bases
TCTATTTTCCGTACCAAACGCCTGTCGGAGTTGTCACGGCAATGATTGGGACGCCGTTTTTTATCTATTTGGCGAGAAGA
CGGAAAAAGGGGGCGGCATA

Downstream 100 bases:

>100_bases
TGCCGTCTTTTTTTAATCTGACAAATGGTGTGAAAGGTTGTAATCCTTCACATGCCATTTATTTTCATGAAATTTGATGT
TGCTTAGGCAGGCATTGACG

Product: YfmE

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: Ferric-citrate import system permease protein [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MNNPARKTKRRRGQSAVVLCALFACLFVLIVLNLAIGDENIGLWDVLKSLAGQGDAGVRFLVMEFRMPRILLAVLAGGAL
SIAGVVAQFLMKNPLAAPDTLGITGGAGLGAVIVTVLFSQQSPLLLTGAAFSGSVLAAILVYLLAYRNGISPVRLALVGI
AVNAFCHSGIQLLISRGSPNVNSALIWLNGSLWGRGWQDVLPLFIWCCLVIPPIWLAAKPLDIIKSGDGIAVGLGIRLER
TRFLLLAGAVLLTAAAVTAVGTIGFIGLMAPHIARKLAGAETKILIPVAALVGGIVLLIADAAGRGLVPPLEIPAGLIAA
VIGGPYFIYLLRREEHKKRRHA

Sequences:

>Translated_342_residues
MNNPARKTKRRRGQSAVVLCALFACLFVLIVLNLAIGDENIGLWDVLKSLAGQGDAGVRFLVMEFRMPRILLAVLAGGAL
SIAGVVAQFLMKNPLAAPDTLGITGGAGLGAVIVTVLFSQQSPLLLTGAAFSGSVLAAILVYLLAYRNGISPVRLALVGI
AVNAFCHSGIQLLISRGSPNVNSALIWLNGSLWGRGWQDVLPLFIWCCLVIPPIWLAAKPLDIIKSGDGIAVGLGIRLER
TRFLLLAGAVLLTAAAVTAVGTIGFIGLMAPHIARKLAGAETKILIPVAALVGGIVLLIADAAGRGLVPPLEIPAGLIAA
VIGGPYFIYLLRREEHKKRRHA
>Mature_342_residues
MNNPARKTKRRRGQSAVVLCALFACLFVLIVLNLAIGDENIGLWDVLKSLAGQGDAGVRFLVMEFRMPRILLAVLAGGAL
SIAGVVAQFLMKNPLAAPDTLGITGGAGLGAVIVTVLFSQQSPLLLTGAAFSGSVLAAILVYLLAYRNGISPVRLALVGI
AVNAFCHSGIQLLISRGSPNVNSALIWLNGSLWGRGWQDVLPLFIWCCLVIPPIWLAAKPLDIIKSGDGIAVGLGIRLER
TRFLLLAGAVLLTAAAVTAVGTIGFIGLMAPHIARKLAGAETKILIPVAALVGGIVLLIADAAGRGLVPPLEIPAGLIAA
VIGGPYFIYLLRREEHKKRRHA

Specific function: Part of the ABC transporter complex yfmCDEF involved in citrate-dependent Fe(3+) import. Involved in the translocation of the substrate across the membrane (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790740, Length=277, Percent_Identity=40.7942238267148, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1786347, Length=279, Percent_Identity=37.2759856630824, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1786804, Length=298, Percent_Identity=36.9127516778523, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1790741, Length=283, Percent_Identity=34.6289752650177, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1786805, Length=310, Percent_Identity=35.4838709677419, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1788004, Length=282, Percent_Identity=31.9148936170213, Blast_Score=110, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000522 [H]

Pfam domain/function: PF01032 FecCD [H]

EC number: NA

Molecular weight: Translated: 35809; Mature: 35809

Theoretical pI: Translated: 10.84; Mature: 10.84

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNPARKTKRRRGQSAVVLCALFACLFVLIVLNLAIGDENIGLWDVLKSLAGQGDAGVRF
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEE
LVMEFRMPRILLAVLAGGALSIAGVVAQFLMKNPLAAPDTLGITGGAGLGAVIVTVLFSQ
EHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCC
QSPLLLTGAAFSGSVLAAILVYLLAYRNGISPVRLALVGIAVNAFCHSGIQLLISRGSPN
CCCEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VNSALIWLNGSLWGRGWQDVLPLFIWCCLVIPPIWLAAKPLDIIKSGDGIAVGLGIRLER
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHCCCHHHHCCCCCEEEEECEEEHH
TRFLLLAGAVLLTAAAVTAVGTIGFIGLMAPHIARKLAGAETKILIPVAALVGGIVLLIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
DAAGRGLVPPLEIPAGLIAAVIGGPYFIYLLRREEHKKRRHA
CCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECHHHHHHCCC
>Mature Secondary Structure
MNNPARKTKRRRGQSAVVLCALFACLFVLIVLNLAIGDENIGLWDVLKSLAGQGDAGVRF
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEE
LVMEFRMPRILLAVLAGGALSIAGVVAQFLMKNPLAAPDTLGITGGAGLGAVIVTVLFSQ
EHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCC
QSPLLLTGAAFSGSVLAAILVYLLAYRNGISPVRLALVGIAVNAFCHSGIQLLISRGSPN
CCCEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VNSALIWLNGSLWGRGWQDVLPLFIWCCLVIPPIWLAAKPLDIIKSGDGIAVGLGIRLER
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCHHHHHCCCHHHHCCCCCEEEEECEEEHH
TRFLLLAGAVLLTAAAVTAVGTIGFIGLMAPHIARKLAGAETKILIPVAALVGGIVLLIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
DAAGRGLVPPLEIPAGLIAAVIGGPYFIYLLRREEHKKRRHA
CCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]