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The map label for this gene is pfpI [H]

Identifier: 52784646

GI number: 52784646

Start: 866801

End: 867574

Strand: Reverse

Name: pfpI [H]

Synonym: BLi00848

Alternate gene names: 52784646

Gene position: 867574-866801 (Counterclockwise)

Preceding gene: 52784652

Following gene: 52784645

Centisome position: 20.55

GC content: 48.45

Gene sequence:

>774_bases
GTGCCTCTTCCATATGAAAAAACCTCCTATCTTTTTTACTCACACGTTATGATGCAAAAATCGTGCCAGCTCTCCGAAGC
GAACATAAGCCGCTTTTCAGGCGGACATCCTTTCAGAACAAGAGACAAAACGAGACAGATGTCTCAATTTGTCTACTTCG
TCAGTTGCGGAAATGTCGTTGCCGCGGTTAGAATGAACGGTAGAGACGAAGGAGGTTTTTTCATGAAGAAAGCGTATGTT
TTTTTAATCGACGGATTCGAAGAAATTGAAGCGATAGCAACAATTGATGTTTTACGAAGGGCAGAAGTTGAGACCGTCAC
CGTCTCGCTTGATCCATCGCGCAGCGTGAAAGGCGGACATGACATTGTCGTCGAGGCAGACGTCATGTTTGACGATGCTG
ATTGGCAAGAAGCGGATATGCTGATTCTTCCGGGCGGAAATGTAGGCAGCAAAAAAATGCTGGAGCATCAAGCTCTGCAT
AAGATGCTGACAGAAGCGGCAAATGCAGGAAAATATGTTGCCGCCATTTGTGCGGCAACGATGACGCTCGGAAAAACGGG
GCTTGTCAGCGGAAAAAAAGCGACCTGCTATCCGGGAGTTGAAGAACATTTAACAGGGGCGGATGTCACCGCACATGAAA
ACGTCGTCGTTGATGGAAATATCATTACTTCCAGAGGGCCGGCGACCACGATCCCGTTTGCCCTCAAGCTTGCCGAACTG
CTGAACGGCAAGGAAAAAGCCGGTGCAGTCGCCAAGGGAATGCTCGTCTGCTAA

Upstream 100 bases:

>100_bases
CCCGGCAGTATTCCTTTAGCAAACAGCTGATGAACCTGATCTTCGAAATAGCGGATTTCCAGCATTTTTTGATACATCCA
CACTGCTTTTTCCTTTGTCA

Downstream 100 bases:

>100_bases
GACGGCATTCCTCTTTTTTATCCAATAAAAGACGGTATTGACATCATAAATCTTAAAAGATATGATATTTAAAAAGTAAT
TCCGTAAAATCAATAATCGT

Product: hypothetical protein

Products: NA

Alternate protein names: Intracellular protease I [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MPLPYEKTSYLFYSHVMMQKSCQLSEANISRFSGGHPFRTRDKTRQMSQFVYFVSCGNVVAAVRMNGRDEGGFFMKKAYV
FLIDGFEEIEAIATIDVLRRAEVETVTVSLDPSRSVKGGHDIVVEADVMFDDADWQEADMLILPGGNVGSKKMLEHQALH
KMLTEAANAGKYVAAICAATMTLGKTGLVSGKKATCYPGVEEHLTGADVTAHENVVVDGNIITSRGPATTIPFALKLAEL
LNGKEKAGAVAKGMLVC

Sequences:

>Translated_257_residues
MPLPYEKTSYLFYSHVMMQKSCQLSEANISRFSGGHPFRTRDKTRQMSQFVYFVSCGNVVAAVRMNGRDEGGFFMKKAYV
FLIDGFEEIEAIATIDVLRRAEVETVTVSLDPSRSVKGGHDIVVEADVMFDDADWQEADMLILPGGNVGSKKMLEHQALH
KMLTEAANAGKYVAAICAATMTLGKTGLVSGKKATCYPGVEEHLTGADVTAHENVVVDGNIITSRGPATTIPFALKLAEL
LNGKEKAGAVAKGMLVC
>Mature_256_residues
PLPYEKTSYLFYSHVMMQKSCQLSEANISRFSGGHPFRTRDKTRQMSQFVYFVSCGNVVAAVRMNGRDEGGFFMKKAYVF
LIDGFEEIEAIATIDVLRRAEVETVTVSLDPSRSVKGGHDIVVEADVMFDDADWQEADMLILPGGNVGSKKMLEHQALHK
MLTEAANAGKYVAAICAATMTLGKTGLVSGKKATCYPGVEEHLTGADVTAHENVVVDGNIITSRGPATTIPFALKLAELL
NGKEKAGAVAKGMLVC

Specific function: Thiamine biosynthesis [C]

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PfpI endopeptidase domain [H]

Homologues:

Organism=Homo sapiens, GI183227678, Length=178, Percent_Identity=37.6404494382022, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI31543380, Length=178, Percent_Identity=37.6404494382022, Blast_Score=108, Evalue=5e-24,
Organism=Escherichia coli, GI87081736, Length=184, Percent_Identity=32.6086956521739, Blast_Score=76, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17531319, Length=183, Percent_Identity=39.344262295082, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17558714, Length=171, Percent_Identity=33.9181286549708, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28571932, Length=183, Percent_Identity=41.5300546448087, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24653499, Length=182, Percent_Identity=38.4615384615385, Blast_Score=110, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006286
- InterPro:   IPR002818 [H]

Pfam domain/function: PF01965 DJ-1_PfpI [H]

EC number: NA

Molecular weight: Translated: 27847; Mature: 27716

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLPYEKTSYLFYSHVMMQKSCQLSEANISRFSGGHPFRTRDKTRQMSQFVYFVSCGNVV
CCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCHHHHHHHHEEEEEECCCEE
AAVRMNGRDEGGFFMKKAYVFLIDGFEEIEAIATIDVLRRAEVETVTVSLDPSRSVKGGH
EEEEECCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
DIVVEADVMFDDADWQEADMLILPGGNVGSKKMLEHQALHKMLTEAANAGKYVAAICAAT
EEEEEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
MTLGKTGLVSGKKATCYPGVEEHLTGADVTAHENVVVDGNIITSRGPATTIPFALKLAEL
HHCCCCCCCCCCCCEECCCHHHHCCCCCCCCCCCEEEECEEEECCCCCCHHHHHHHHHHH
LNGKEKAGAVAKGMLVC
HCCCHHCCHHHCCEECC
>Mature Secondary Structure 
PLPYEKTSYLFYSHVMMQKSCQLSEANISRFSGGHPFRTRDKTRQMSQFVYFVSCGNVV
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCHHHHHHHHEEEEEECCCEE
AAVRMNGRDEGGFFMKKAYVFLIDGFEEIEAIATIDVLRRAEVETVTVSLDPSRSVKGGH
EEEEECCCCCCCEEEEEEEEEEECCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
DIVVEADVMFDDADWQEADMLILPGGNVGSKKMLEHQALHKMLTEAANAGKYVAAICAAT
EEEEEEEEEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
MTLGKTGLVSGKKATCYPGVEEHLTGADVTAHENVVVDGNIITSRGPATTIPFALKLAEL
HHCCCCCCCCCCCCEECCCHHHHCCCCCCCCCCCEEEECEEEECCCCCCHHHHHHHHHHH
LNGKEKAGAVAKGMLVC
HCCCHHCCHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]