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The map label for this gene is bioB [H]

Identifier: 52784569

GI number: 52784569

Start: 781514

End: 782515

Strand: Direct

Name: bioB [H]

Synonym: BLi00770

Alternate gene names: 52784569

Gene position: 781514-782515 (Clockwise)

Preceding gene: 52784568

Following gene: 52784570

Centisome position: 18.51

GC content: 48.6

Gene sequence:

>1002_bases
ATGAATCAGTGGATGGAACTTGCAGAACGTGTGCTGGATGGCGGGGAAGTAACTGAAAAAGAGGCGCTTTCCATCTTGGA
ATGCCCGGATGACGACGTTCTTCTGCTTATGCATGCCGCTTTTCAAATCAGAAAACGCTACTACGGAAAAAAAGTAAAGC
TCAATATGATTATGAATGCAAAATCCGGCCTTTGTCCGGAAAACTGCGGCTACTGCTCGCAGTCTTCGATATCAAAAGCG
CCGATCGACAGCTACCGGATGGTCGACAAAACGACCTTGCTTGAAGGCGCAAAGCGTGCGCACGATTTAAATATCGGAAC
ATACTGCATCGTGGCGAGCGGCAGAGGACCGTCCAACAGAGAAGTCGATCAAGTGGTCGACGCCGTTAAAGAAATCAAAG
AAACGTACGGTTTAAAAATCTGCGCATGCCTCGGACTGCTAAAGCCCGGGCAAGCCGAGCGGCTGAAGGAAGCCGGAGTA
GACCGGTACAATCACAACATCAATACATCAAAAACCAACCATTCAAATATTACAACGTCCCACACGTATGATGACAGGGT
GAACACTGTGGAAACAGCCAAAAAATCCGGGATGTCCCCATGTTCGGGCGTCATCGTCGGGATGAAAGAGACGAAGCAGG
ATGTCGTTGATATGGCGAAAAGCTTAAAGGCGCTGGATGCCGACTCGATTCCAGTTAATTTTTTGCATGCGATCGACGGG
ACTCCGCTGGAAGGGGTCAATGAATTGAATCCGCTTTATTGTTTAAAGGTTTTAGCTTTGTTCCGCTTTATCAACCCGAC
AAAGGAAATCCGCATTTCAGGGGGGCGCGAAGTCAACCTCCGCTCCTTGCAGCCGTTGGGGCTGTATGCCGCCAATTCAA
TCTTCGTCGGAGACTATTTAACGACGGCAGGACAGAATGAAACAGAGGACCATAAGATGCTTCATGATTTAGGGTTCGAA
GTCGAATCGGTCGAAGAAATGAAAGCCAGTTTACAGCGGTAG

Upstream 100 bases:

>100_bases
CGAAGCTTGAAGACGCTTCACCAACACAAATTCACCACATGATCAAGGATCATGTCGATGTTTCCTTGATTATGAATCAA
ATGCAAATGGGGGCAGAAAA

Downstream 100 bases:

>100_bases
CACGGGGGAGATATAACATGACGACAGAACTGCACGAGGCAGGGCCGAGCCTGAAATCGGATTCGGATTTTTGGAGAGAT
CCATATCTGTTTTACGATCA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MNQWMELAERVLDGGEVTEKEALSILECPDDDVLLLMHAAFQIRKRYYGKKVKLNMIMNAKSGLCPENCGYCSQSSISKA
PIDSYRMVDKTTLLEGAKRAHDLNIGTYCIVASGRGPSNREVDQVVDAVKEIKETYGLKICACLGLLKPGQAERLKEAGV
DRYNHNINTSKTNHSNITTSHTYDDRVNTVETAKKSGMSPCSGVIVGMKETKQDVVDMAKSLKALDADSIPVNFLHAIDG
TPLEGVNELNPLYCLKVLALFRFINPTKEIRISGGREVNLRSLQPLGLYAANSIFVGDYLTTAGQNETEDHKMLHDLGFE
VESVEEMKASLQR

Sequences:

>Translated_333_residues
MNQWMELAERVLDGGEVTEKEALSILECPDDDVLLLMHAAFQIRKRYYGKKVKLNMIMNAKSGLCPENCGYCSQSSISKA
PIDSYRMVDKTTLLEGAKRAHDLNIGTYCIVASGRGPSNREVDQVVDAVKEIKETYGLKICACLGLLKPGQAERLKEAGV
DRYNHNINTSKTNHSNITTSHTYDDRVNTVETAKKSGMSPCSGVIVGMKETKQDVVDMAKSLKALDADSIPVNFLHAIDG
TPLEGVNELNPLYCLKVLALFRFINPTKEIRISGGREVNLRSLQPLGLYAANSIFVGDYLTTAGQNETEDHKMLHDLGFE
VESVEEMKASLQR
>Mature_333_residues
MNQWMELAERVLDGGEVTEKEALSILECPDDDVLLLMHAAFQIRKRYYGKKVKLNMIMNAKSGLCPENCGYCSQSSISKA
PIDSYRMVDKTTLLEGAKRAHDLNIGTYCIVASGRGPSNREVDQVVDAVKEIKETYGLKICACLGLLKPGQAERLKEAGV
DRYNHNINTSKTNHSNITTSHTYDDRVNTVETAKKSGMSPCSGVIVGMKETKQDVVDMAKSLKALDADSIPVNFLHAIDG
TPLEGVNELNPLYCLKVLALFRFINPTKEIRISGGREVNLRSLQPLGLYAANSIFVGDYLTTAGQNETEDHKMLHDLGFE
VESVEEMKASLQR

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=303, Percent_Identity=33.6633663366337, Blast_Score=181, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6321725, Length=304, Percent_Identity=33.5526315789474, Blast_Score=158, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 36821; Mature: 36821

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQWMELAERVLDGGEVTEKEALSILECPDDDVLLLMHAAFQIRKRYYGKKVKLNMIMNA
CCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECC
KSGLCPENCGYCSQSSISKAPIDSYRMVDKTTLLEGAKRAHDLNIGTYCIVASGRGPSNR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
EVDQVVDAVKEIKETYGLKICACLGLLKPGQAERLKEAGVDRYNHNINTSKTNHSNITTS
CHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCCC
HTYDDRVNTVETAKKSGMSPCSGVIVGMKETKQDVVDMAKSLKALDADSIPVNFLHAIDG
CCCCCHHHHHHHHHHCCCCCCHHEEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
TPLEGVNELNPLYCLKVLALFRFINPTKEIRISGGREVNLRSLQPLGLYAANSIFVGDYL
CCCCCHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCEECCCCCCCCCEEECCCEEECCHH
TTAGQNETEDHKMLHDLGFEVESVEEMKASLQR
HCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNQWMELAERVLDGGEVTEKEALSILECPDDDVLLLMHAAFQIRKRYYGKKVKLNMIMNA
CCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECC
KSGLCPENCGYCSQSSISKAPIDSYRMVDKTTLLEGAKRAHDLNIGTYCIVASGRGPSNR
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCC
EVDQVVDAVKEIKETYGLKICACLGLLKPGQAERLKEAGVDRYNHNINTSKTNHSNITTS
CHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCCC
HTYDDRVNTVETAKKSGMSPCSGVIVGMKETKQDVVDMAKSLKALDADSIPVNFLHAIDG
CCCCCHHHHHHHHHHCCCCCCHHEEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
TPLEGVNELNPLYCLKVLALFRFINPTKEIRISGGREVNLRSLQPLGLYAANSIFVGDYL
CCCCCHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCEECCCCCCCCCEEECCCEEECCHH
TTAGQNETEDHKMLHDLGFEVESVEEMKASLQR
HCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA