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The map label for this gene is bioD

Identifier: 52784568

GI number: 52784568

Start: 780810

End: 781517

Strand: Direct

Name: bioD

Synonym: BLi00769

Alternate gene names: 52784568

Gene position: 780810-781517 (Clockwise)

Preceding gene: 52784567

Following gene: 52784569

Centisome position: 18.49

GC content: 50.0

Gene sequence:

>708_bases
ATGAAGGGTTTTTTTGTAACGGGAACAGATACGGGAGTCGGGAAGACGTTTATCGCTTGCGGTCTTGCCGCTTTACTGAA
AGAGCAGCATGTAGATGTCGGCGTGTTTAAGCCGTTTTTGAGCGGCGAGCTCGCTAGCGATCCGCAGAGTGATACAGCTC
TTTTAAAGAACATGTCTGAAACGCCTCTTACTGATGAAGAGGTTACGCCTTTTATTTTTAAAGAGCCGCTTGCACCATAC
ACAGCTGCGAAGCTCGAAGGGAGGACAGTCGGGCTGGAAGATGCCGTGAACCATTGGAAAAAGATAAAGGATCGGCACGA
GTGCTTCATCGTTGAAGGGGCAGGCGGAATTGCTGTGCCGCTCGGGGAGGATTATTTCGTCAGCGACTTGATCAAAGCGC
TCGATTTGCCGGCCGTCATTGTGGCGCGTCCCAATCTCGGAACGATCAACCATACGTATTTAACCGCCCAATACGCAAAA
CAAATGGGGATACGCGTGATCGGCATCGTGATCAACGGCATCAGCAGCCGGCCGGGACTTGACGAACAGACGAATCCGGA
CATGATTGAAAGATTTTGCGGTGTGCCGCTCCTCGGCGTTACACCGAAGCTTGAAGACGCTTCACCAACACAAATTCACC
ACATGATCAAGGATCATGTCGATGTTTCCTTGATTATGAATCAAATGCAAATGGGGGCAGAAAAATGA

Upstream 100 bases:

>100_bases
GCCGGATCAGGCTGACCGTCACCGCAGAACGCAGGTTGAAAGATATTGAAGATCTATTAGAGGGGTTTAAATTAATGGGA
AGAGAGTTGAACTTGGTGAA

Downstream 100 bases:

>100_bases
ATCAGTGGATGGAACTTGCAGAACGTGTGCTGGATGGCGGGGAAGTAACTGAAAAAGAGGCGCTTTCCATCTTGGAATGC
CCGGATGACGACGTTCTTCT

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MKGFFVTGTDTGVGKTFIACGLAALLKEQHVDVGVFKPFLSGELASDPQSDTALLKNMSETPLTDEEVTPFIFKEPLAPY
TAAKLEGRTVGLEDAVNHWKKIKDRHECFIVEGAGGIAVPLGEDYFVSDLIKALDLPAVIVARPNLGTINHTYLTAQYAK
QMGIRVIGIVINGISSRPGLDEQTNPDMIERFCGVPLLGVTPKLEDASPTQIHHMIKDHVDVSLIMNQMQMGAEK

Sequences:

>Translated_235_residues
MKGFFVTGTDTGVGKTFIACGLAALLKEQHVDVGVFKPFLSGELASDPQSDTALLKNMSETPLTDEEVTPFIFKEPLAPY
TAAKLEGRTVGLEDAVNHWKKIKDRHECFIVEGAGGIAVPLGEDYFVSDLIKALDLPAVIVARPNLGTINHTYLTAQYAK
QMGIRVIGIVINGISSRPGLDEQTNPDMIERFCGVPLLGVTPKLEDASPTQIHHMIKDHVDVSLIMNQMQMGAEK
>Mature_235_residues
MKGFFVTGTDTGVGKTFIACGLAALLKEQHVDVGVFKPFLSGELASDPQSDTALLKNMSETPLTDEEVTPFIFKEPLAPY
TAAKLEGRTVGLEDAVNHWKKIKDRHECFIVEGAGGIAVPLGEDYFVSDLIKALDLPAVIVARPNLGTINHTYLTAQYAK
QMGIRVIGIVINGISSRPGLDEQTNPDMIERFCGVPLLGVTPKLEDASPTQIHHMIKDHVDVSLIMNQMQMGAEK

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=208, Percent_Identity=32.2115384615385, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI87081944, Length=227, Percent_Identity=28.6343612334802, Blast_Score=74, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6324385, Length=184, Percent_Identity=35.8695652173913, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_BACLD (Q65ML0)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_077993.1
- RefSeq:   YP_090397.1
- HSSP:   P13000
- ProteinModelPortal:   Q65ML0
- SMR:   Q65ML0
- STRING:   Q65ML0
- EnsemblBacteria:   EBBACT00000056030
- EnsemblBacteria:   EBBACT00000060235
- GeneID:   3029556
- GeneID:   3098999
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00769
- KEGG:   bli:BL00955
- NMPDR:   fig|279010.5.peg.1215
- eggNOG:   COG0132
- GeneTree:   EBGT00050000001962
- HOGENOM:   HBG650065
- OMA:   ANCVDPN
- ProtClustDB:   PRK00090
- BioCyc:   BLIC279010-1:BLI00769-MONOMER
- BioCyc:   BLIC279010:BL00955-MONOMER
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 25530; Mature: 25530

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGFFVTGTDTGVGKTFIACGLAALLKEQHVDVGVFKPFLSGELASDPQSDTALLKNMSE
CCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHCCCC
TPLTDEEVTPFIFKEPLAPYTAAKLEGRTVGLEDAVNHWKKIKDRHECFIVEGAGGIAVP
CCCCCCCCCCHHHHCCCCCHHHHHCCCCEECHHHHHHHHHHHCCCCCEEEEECCCCEEEE
LGEDYFVSDLIKALDLPAVIVARPNLGTINHTYLTAQYAKQMGIRVIGIVINGISSRPGL
CCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
DEQTNPDMIERFCGVPLLGVTPKLEDASPTQIHHMIKDHVDVSLIMNQMQMGAEK
CCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKGFFVTGTDTGVGKTFIACGLAALLKEQHVDVGVFKPFLSGELASDPQSDTALLKNMSE
CCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHCCCC
TPLTDEEVTPFIFKEPLAPYTAAKLEGRTVGLEDAVNHWKKIKDRHECFIVEGAGGIAVP
CCCCCCCCCCHHHHCCCCCHHHHHCCCCEECHHHHHHHHHHHCCCCCEEEEECCCCEEEE
LGEDYFVSDLIKALDLPAVIVARPNLGTINHTYLTAQYAKQMGIRVIGIVINGISSRPGL
CCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCC
DEQTNPDMIERFCGVPLLGVTPKLEDASPTQIHHMIKDHVDVSLIMNQMQMGAEK
CCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA