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The map label for this gene is bioA [H]

Identifier: 52784566

GI number: 52784566

Start: 778338

End: 779684

Strand: Direct

Name: bioA [H]

Synonym: BLi00767

Alternate gene names: 52784566

Gene position: 778338-779684 (Clockwise)

Preceding gene: 52784565

Following gene: 52784567

Centisome position: 18.43

GC content: 51.08

Gene sequence:

>1347_bases
ATGAATCAATCATTCGTTGAGAAAAGCAAACAATATCTTTGGCTGCCTTTTACCCAAATGAAAGACTATGACGAAAACCC
GCTGATCATCGAAAGCGGGCAGGGAATCAAGCTGAAAGATATCGACGGGAGGGTGTACTACGACGGATTTTCTTCAGTAT
GGCTCAATGTCCATGGCCATCGCAAGAAGGAACTGGATGAGGCCATCAAAAAACAGCTCGGCAAAATCGCTCATTCGACA
TTGCTGGGAATGACAAATGTTCCGGCGACAGAACTTGCCGAGGTGTTAATCAAGATCACGCCGGAAAATCTGACGCGTGT
TTTTTATTCGGACAGCGGCGCCACAGCAATGGAAATCGCGCTGAAGATGGCCTTTCAATATTGGAAAAACATCGGCAAGC
CGGAGAAACAAAAATTCATTTCGATGAAAAACGGCTATCACGGCGATACGATCGGCGCTGTCAGCGTCGGTGCAATCGAG
CTCTTCCACCATGTATACGGGCCGCTTATGTTTGAAAGCTTTAAGGTGAACGTTCCATACGTATACCGCTCGAAAAGCGG
CAACCCGGACGAGTGCCGCGATGAATGTCTCGCAGAACTCGAACGGCTCCTGAGCGAGCGGCACGATGAGATCGCCGCGC
TGTCGGTCGAATCGATGGTTCAAGGGGCATCAGGGATGATCGTGATGCCTGAAGGATATTTGGCAGGCGTCCGGGAGCTC
TGCACGAAATATGACGTTTTAATGATTGTCGATGAAGTGGCGACAGGGTTCGGCCGAACCGGGAAAATGTTTGCCTGTGA
GCATGAACGTGTTCAACCTGACTTGATGGCGGCTGGAAAGGCCATTACCGGAGGCTATTTGCCGATCGCCGTAACGTTTG
CGACAGAGGAAATTTATGAGGCGTTCTATGATGACTACAACAAACTCAAAACGTTTTTCCACGGCCACTCATACACGGGG
AACCAGCTTGGTTGTGCCGTTGCGCTGGAAAATTTGCGCCTGTTCGAATCGGAAAAAATCGTCGCTCAAGTTGCGGAAAA
GTCGAAAATACTGGAATCGCTCTTTCATGATCTGGCAGCGCTCCCTCATGTCGGCGACATCCGCCAGCTCGGTTTTATGA
GCGGGATCGAGCTCGTTCAGTCGAAGGAAACGAGGCAGCCTTATCCGCCCGAAGAGCGAATCGGATACCGGGTGTCTTTG
AAAATGAGAGAGCTTGGCATGCTGACGAGGCCGCTCGGGGATGTTGTCGCATTTCTTCCTCCGCTCGCCAGCACCGCCGA
TGACCTGCGCGCCATGGTGTCGATTATGAAAGAAGCGATTCAAGAGGTTACAGGCCGTGCGTATTGA

Upstream 100 bases:

>100_bases
AGCGGCTGCCGGATTTTTTTTGTTGATGGATCAGCAGATGTAAAAAGCTGCATAGACGATTTGGAAAAACAGCCTGTATT
CATTGGTCAGGAGGTAAATC

Downstream 100 bases:

>100_bases
CGAATGGCTCAGCAGCCGGCTCGCAAAGACGAAAGCAGCCGGGCTTTACCGCTCGCTTAAACTGCCTCAAGCTGAACGTA
CGAATTGGGCGTCAAACGAT

Product: adenosylmethionine--8-amino-7-oxononanoate transaminase

Products: S-adenosyl-4-methylthio-2-oxobutanoate; 7,8-diaminononanoate

Alternate protein names: 7,8-diamino-pelargonic acid aminotransferase; DAPA aminotransferase [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MNQSFVEKSKQYLWLPFTQMKDYDENPLIIESGQGIKLKDIDGRVYYDGFSSVWLNVHGHRKKELDEAIKKQLGKIAHST
LLGMTNVPATELAEVLIKITPENLTRVFYSDSGATAMEIALKMAFQYWKNIGKPEKQKFISMKNGYHGDTIGAVSVGAIE
LFHHVYGPLMFESFKVNVPYVYRSKSGNPDECRDECLAELERLLSERHDEIAALSVESMVQGASGMIVMPEGYLAGVREL
CTKYDVLMIVDEVATGFGRTGKMFACEHERVQPDLMAAGKAITGGYLPIAVTFATEEIYEAFYDDYNKLKTFFHGHSYTG
NQLGCAVALENLRLFESEKIVAQVAEKSKILESLFHDLAALPHVGDIRQLGFMSGIELVQSKETRQPYPPEERIGYRVSL
KMRELGMLTRPLGDVVAFLPPLASTADDLRAMVSIMKEAIQEVTGRAY

Sequences:

>Translated_448_residues
MNQSFVEKSKQYLWLPFTQMKDYDENPLIIESGQGIKLKDIDGRVYYDGFSSVWLNVHGHRKKELDEAIKKQLGKIAHST
LLGMTNVPATELAEVLIKITPENLTRVFYSDSGATAMEIALKMAFQYWKNIGKPEKQKFISMKNGYHGDTIGAVSVGAIE
LFHHVYGPLMFESFKVNVPYVYRSKSGNPDECRDECLAELERLLSERHDEIAALSVESMVQGASGMIVMPEGYLAGVREL
CTKYDVLMIVDEVATGFGRTGKMFACEHERVQPDLMAAGKAITGGYLPIAVTFATEEIYEAFYDDYNKLKTFFHGHSYTG
NQLGCAVALENLRLFESEKIVAQVAEKSKILESLFHDLAALPHVGDIRQLGFMSGIELVQSKETRQPYPPEERIGYRVSL
KMRELGMLTRPLGDVVAFLPPLASTADDLRAMVSIMKEAIQEVTGRAY
>Mature_448_residues
MNQSFVEKSKQYLWLPFTQMKDYDENPLIIESGQGIKLKDIDGRVYYDGFSSVWLNVHGHRKKELDEAIKKQLGKIAHST
LLGMTNVPATELAEVLIKITPENLTRVFYSDSGATAMEIALKMAFQYWKNIGKPEKQKFISMKNGYHGDTIGAVSVGAIE
LFHHVYGPLMFESFKVNVPYVYRSKSGNPDECRDECLAELERLLSERHDEIAALSVESMVQGASGMIVMPEGYLAGVREL
CTKYDVLMIVDEVATGFGRTGKMFACEHERVQPDLMAAGKAITGGYLPIAVTFATEEIYEAFYDDYNKLKTFFHGHSYTG
NQLGCAVALENLRLFESEKIVAQVAEKSKILESLFHDLAALPHVGDIRQLGFMSGIELVQSKETRQPYPPEERIGYRVSL
KMRELGMLTRPLGDVVAFLPPLASTADDLRAMVSIMKEAIQEVTGRAY

Specific function: Catalyzes the formation of the intermediate 7,8- diaminopelargonic acid (DAPA) from 7-keto-8-aminopelargonic acid (KAPA) with lysine as the amino donor. B.subtilis does not utilize S-adenosylmethionine (SAM), but instead utilizes only L-lysine as the amin

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=424, Percent_Identity=31.6037735849057, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI284507298, Length=333, Percent_Identity=32.7327327327327, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI13994255, Length=390, Percent_Identity=29.2307692307692, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI37574042, Length=431, Percent_Identity=28.7703016241299, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI226442705, Length=430, Percent_Identity=28.8372093023256, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI226442709, Length=362, Percent_Identity=30.1104972375691, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI24119277, Length=431, Percent_Identity=26.2180974477958, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1786991, Length=439, Percent_Identity=34.624145785877, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI145693181, Length=419, Percent_Identity=32.6968973747017, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1788044, Length=429, Percent_Identity=29.1375291375291, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1789759, Length=425, Percent_Identity=28.4705882352941, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789016, Length=449, Percent_Identity=24.7216035634744, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787560, Length=420, Percent_Identity=26.4285714285714, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786349, Length=374, Percent_Identity=24.5989304812834, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25144271, Length=426, Percent_Identity=26.7605633802817, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI32564660, Length=453, Percent_Identity=29.3598233995585, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI25144274, Length=303, Percent_Identity=29.3729372937294, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI71992977, Length=442, Percent_Identity=24.6606334841629, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17541228, Length=244, Percent_Identity=27.4590163934426, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324386, Length=467, Percent_Identity=29.7644539614561, Blast_Score=199, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6324432, Length=429, Percent_Identity=31.002331002331, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6323470, Length=451, Percent_Identity=25.2771618625277, Blast_Score=143, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6321456, Length=302, Percent_Identity=26.4900662251656, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357415, Length=428, Percent_Identity=29.6728971962617, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI161085790, Length=443, Percent_Identity=28.4424379232506, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI28574759, Length=445, Percent_Identity=28.5393258426966, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI21356575, Length=443, Percent_Identity=26.1851015801354, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI281366494, Length=369, Percent_Identity=23.0352303523035, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24667139, Length=369, Percent_Identity=23.0352303523035, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24667143, Length=369, Percent_Identity=23.0352303523035, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.62

Molecular weight: Translated: 50215; Mature: 50215

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQSFVEKSKQYLWLPFTQMKDYDENPLIIESGQGIKLKDIDGRVYYDGFSSVWLNVHGH
CCCHHHHHCCCEEEEEHHHHCCCCCCCEEEECCCCEEEEECCCEEEECCCCEEEEEECCC
RKKELDEAIKKQLGKIAHSTLLGMTNVPATELAEVLIKITPENLTRVFYSDSGATAMEIA
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHEEEEECCCCCHHHHHH
LKMAFQYWKNIGKPEKQKFISMKNGYHGDTIGAVSVGAIELFHHVYGPLMFESFKVNVPY
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCE
VYRSKSGNPDECRDECLAELERLLSERHDEIAALSVESMVQGASGMIVMPEGYLAGVREL
EEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHH
CTKYDVLMIVDEVATGFGRTGKMFACEHERVQPDLMAAGKAITGGYLPIAVTFATEEIYE
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHCCCCCCCCCEEEEEEEHHHHHHH
AFYDDYNKLKTFFHGHSYTGNQLGCAVALENLRLFESEKIVAQVAEKSKILESLFHDLAA
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPHVGDIRQLGFMSGIELVQSKETRQPYPPEERIGYRVSLKMRELGMLTRPLGDVVAFLP
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCEEEEEHHHHHHHHHCCHHHHHHHHC
PLASTADDLRAMVSIMKEAIQEVTGRAY
CCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNQSFVEKSKQYLWLPFTQMKDYDENPLIIESGQGIKLKDIDGRVYYDGFSSVWLNVHGH
CCCHHHHHCCCEEEEEHHHHCCCCCCCEEEECCCCEEEEECCCEEEECCCCEEEEEECCC
RKKELDEAIKKQLGKIAHSTLLGMTNVPATELAEVLIKITPENLTRVFYSDSGATAMEIA
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHEEEEECCCCCHHHHHH
LKMAFQYWKNIGKPEKQKFISMKNGYHGDTIGAVSVGAIELFHHVYGPLMFESFKVNVPY
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCE
VYRSKSGNPDECRDECLAELERLLSERHDEIAALSVESMVQGASGMIVMPEGYLAGVREL
EEECCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHH
CTKYDVLMIVDEVATGFGRTGKMFACEHERVQPDLMAAGKAITGGYLPIAVTFATEEIYE
HHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHCCCCCCCCCEEEEEEEHHHHHHH
AFYDDYNKLKTFFHGHSYTGNQLGCAVALENLRLFESEKIVAQVAEKSKILESLFHDLAA
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPHVGDIRQLGFMSGIELVQSKETRQPYPPEERIGYRVSLKMRELGMLTRPLGDVVAFLP
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCEEEEEHHHHHHHHHCCHHHHHHHHC
PLASTADDLRAMVSIMKEAIQEVTGRAY
CCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; 8-amino-7-oxononanoate

Specific reaction: S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate

General reaction: Amino group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8763940; 9387221; 9384377 [H]