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The map label for this gene is bioF

Identifier: 52784567

GI number: 52784567

Start: 779674

End: 780813

Strand: Direct

Name: bioF

Synonym: BLi00768

Alternate gene names: 52784567

Gene position: 779674-780813 (Clockwise)

Preceding gene: 52784566

Following gene: 52784568

Centisome position: 18.46

GC content: 52.11

Gene sequence:

>1140_bases
GTGCGTATTGACGAATGGCTCAGCAGCCGGCTCGCAAAGACGAAAGCAGCCGGGCTTTACCGCTCGCTTAAACTGCCTCA
AGCTGAACGTACGAATTGGGCGTCAAACGATTATTTGGGCCTGGCGAACGACAAACGGCTGATTCATGCCGCAGAAACAG
CGCTCCGCCGCTTTGGAGCCGGAAGCACAGGATCAAGGCTGACGTCAGGCAATACCGCATGGCATGAAAAACTTGAGCGG
AAAATCGCCGGTTTTAAGCAGACGGAAGCGGCTCTCTTGTTTTCAAGCGGATATTTGGCCAATGTCGGCGTGCTTTCGTC
TTTGCCTGAAAAAGGAGACGTCATATTAAGCGATCAATTGAACCACGCCAGCATCATCGACGGCTGCCGCCTTTCAAAAG
CTGATACGGTTGTTTACCGCCACAAGGATATGAATGATCTTGAAGAAAAGCTCCGCGCCGCACAGAGCCGTGCACGCCGC
TTTATTGTGACAGACGGCGTGTTCAGCATGGACGGCACGATTGCGCCGCTTGACGAAATCATGCTGCTCGCCAAGCAGTA
CCGGGCCTTTGTTATCGTAGATGATGCCCATGCAACAGGGGTTTTGGGAGAAGCCGGCAGGGGAACGAGCGAGTATTTCG
GCGTCTCTCCCGATGTTGTGATCGGTACGTTAAGTAAAGCTGTAGGCGCAGAAGGCGGCTTCGTCGCAGGTTCTAAAACA
TTGATCGATTTTTTGCTGAATCATGCGAGAACGTTTATCTTTCAAACAGCTGTCCCGCCCGCAAGCTGTGCGGCGGCATG
CAAAGCTTTAGATATCATCGAAGACAGCCGGGCTAAACGCCGGCTTTTGCAATCATCGGTGAACACGATCAAACGGAGTC
TCGTGGATATCGGGTTTACGGTCAACGGAGAAGATACGCCGATCATACCCGTTATGATCGGAGATCCCCAAAAAGCTGTT
CAATTTGCAAACGGCCTGAAGGAGAAGGGGATTGAAGCCCCGGCCATCCGACCGCCGACTGTTGCAGAAGGAGAGAGCCG
GATCAGGCTGACCGTCACCGCAGAACGCAGGTTGAAAGATATTGAAGATCTATTAGAGGGGTTTAAATTAATGGGAAGAG
AGTTGAACTTGGTGAAATGA

Upstream 100 bases:

>100_bases
GGGGATGTTGTCGCATTTCTTCCTCCGCTCGCCAGCACCGCCGATGACCTGCGCGCCATGGTGTCGATTATGAAAGAAGC
GATTCAAGAGGTTACAGGCC

Downstream 100 bases:

>100_bases
AGGGTTTTTTTGTAACGGGAACAGATACGGGAGTCGGGAAGACGTTTATCGCTTGCGGTCTTGCCGCTTTACTGAAAGAG
CAGCATGTAGATGTCGGCGT

Product: hypothetical protein

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MRIDEWLSSRLAKTKAAGLYRSLKLPQAERTNWASNDYLGLANDKRLIHAAETALRRFGAGSTGSRLTSGNTAWHEKLER
KIAGFKQTEAALLFSSGYLANVGVLSSLPEKGDVILSDQLNHASIIDGCRLSKADTVVYRHKDMNDLEEKLRAAQSRARR
FIVTDGVFSMDGTIAPLDEIMLLAKQYRAFVIVDDAHATGVLGEAGRGTSEYFGVSPDVVIGTLSKAVGAEGGFVAGSKT
LIDFLLNHARTFIFQTAVPPASCAAACKALDIIEDSRAKRRLLQSSVNTIKRSLVDIGFTVNGEDTPIIPVMIGDPQKAV
QFANGLKEKGIEAPAIRPPTVAEGESRIRLTVTAERRLKDIEDLLEGFKLMGRELNLVK

Sequences:

>Translated_379_residues
MRIDEWLSSRLAKTKAAGLYRSLKLPQAERTNWASNDYLGLANDKRLIHAAETALRRFGAGSTGSRLTSGNTAWHEKLER
KIAGFKQTEAALLFSSGYLANVGVLSSLPEKGDVILSDQLNHASIIDGCRLSKADTVVYRHKDMNDLEEKLRAAQSRARR
FIVTDGVFSMDGTIAPLDEIMLLAKQYRAFVIVDDAHATGVLGEAGRGTSEYFGVSPDVVIGTLSKAVGAEGGFVAGSKT
LIDFLLNHARTFIFQTAVPPASCAAACKALDIIEDSRAKRRLLQSSVNTIKRSLVDIGFTVNGEDTPIIPVMIGDPQKAV
QFANGLKEKGIEAPAIRPPTVAEGESRIRLTVTAERRLKDIEDLLEGFKLMGRELNLVK
>Mature_379_residues
MRIDEWLSSRLAKTKAAGLYRSLKLPQAERTNWASNDYLGLANDKRLIHAAETALRRFGAGSTGSRLTSGNTAWHEKLER
KIAGFKQTEAALLFSSGYLANVGVLSSLPEKGDVILSDQLNHASIIDGCRLSKADTVVYRHKDMNDLEEKLRAAQSRARR
FIVTDGVFSMDGTIAPLDEIMLLAKQYRAFVIVDDAHATGVLGEAGRGTSEYFGVSPDVVIGTLSKAVGAEGGFVAGSKT
LIDFLLNHARTFIFQTAVPPASCAAACKALDIIEDSRAKRRLLQSSVNTIKRSLVDIGFTVNGEDTPIIPVMIGDPQKAV
QFANGLKEKGIEAPAIRPPTVAEGESRIRLTVTAERRLKDIEDLLEGFKLMGRELNLVK

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family

Homologues:

Organism=Homo sapiens, GI284448556, Length=345, Percent_Identity=43.1884057971014, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI7657118, Length=345, Percent_Identity=43.1884057971014, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI83977440, Length=341, Percent_Identity=36.0703812316716, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI83977442, Length=345, Percent_Identity=35.9420289855072, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI83977444, Length=341, Percent_Identity=36.0703812316716, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI4758668, Length=365, Percent_Identity=35.3424657534247, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI4502025, Length=354, Percent_Identity=36.1581920903955, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI40316939, Length=354, Percent_Identity=36.1581920903955, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI119220554, Length=366, Percent_Identity=33.0601092896175, Blast_Score=205, Evalue=6e-53,
Organism=Homo sapiens, GI5454084, Length=328, Percent_Identity=30.1829268292683, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1790046, Length=391, Percent_Identity=38.3631713554987, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1786993, Length=353, Percent_Identity=37.9603399433428, Blast_Score=229, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI71994529, Length=388, Percent_Identity=38.4020618556701, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI32566772, Length=368, Percent_Identity=32.0652173913043, Blast_Score=204, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17560914, Length=361, Percent_Identity=30.1939058171745, Blast_Score=180, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI17560912, Length=361, Percent_Identity=30.1939058171745, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71982617, Length=279, Percent_Identity=28.3154121863799, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI71982625, Length=279, Percent_Identity=28.3154121863799, Blast_Score=132, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6320438, Length=362, Percent_Identity=33.4254143646409, Blast_Score=192, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6320267, Length=366, Percent_Identity=32.5136612021858, Blast_Score=189, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6323954, Length=266, Percent_Identity=29.6992481203008, Blast_Score=143, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24662918, Length=394, Percent_Identity=38.3248730964467, Blast_Score=288, Evalue=3e-78,
Organism=Drosophila melanogaster, GI17137420, Length=346, Percent_Identity=38.150289017341, Blast_Score=228, Evalue=7e-60,
Organism=Drosophila melanogaster, GI17136286, Length=367, Percent_Identity=31.6076294277929, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24653280, Length=304, Percent_Identity=28.2894736842105, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24653276, Length=304, Percent_Identity=28.2894736842105, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24653278, Length=304, Percent_Identity=28.2894736842105, Blast_Score=141, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_BACLD (Q65ML1)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_077992.1
- RefSeq:   YP_090396.1
- HSSP:   P0AB77
- ProteinModelPortal:   Q65ML1
- SMR:   Q65ML1
- STRING:   Q65ML1
- EnsemblBacteria:   EBBACT00000056638
- EnsemblBacteria:   EBBACT00000062571
- GeneID:   3029552
- GeneID:   3099013
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00768
- KEGG:   bli:BL00954
- NMPDR:   fig|279010.5.peg.1118
- eggNOG:   COG0156
- GeneTree:   EBGT00050000000589
- HOGENOM:   HBG649839
- OMA:   IQAIRYP
- ProtClustDB:   CLSK887748
- BioCyc:   BLIC279010-1:BLI00768-MONOMER
- BioCyc:   BLIC279010:BL00954-MONOMER
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 41210; Mature: 41210

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 21-21 BINDING 122-122 BINDING 169-169 BINDING 340-340

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDEWLSSRLAKTKAAGLYRSLKLPQAERTNWASNDYLGLANDKRLIHAAETALRRFGA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCC
GSTGSRLTSGNTAWHEKLERKIAGFKQTEAALLFSSGYLANVGVLSSLPEKGDVILSDQL
CCCCCCCCCCCHHHHHHHHHHHCCCCHHHHEEEEECCCHHHHHHHHCCCCCCCEEEECCC
NHASIIDGCRLSKADTVVYRHKDMNDLEEKLRAAQSRARRFIVTDGVFSMDGTIAPLDEI
CCHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEEECCEEECCCCCCCHHHH
MLLAKQYRAFVIVDDAHATGVLGEAGRGTSEYFGVSPDVVIGTLSKAVGAEGGFVAGSKT
HHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHCCCCCEECCHHH
LIDFLLNHARTFIFQTAVPPASCAAACKALDIIEDSRAKRRLLQSSVNTIKRSLVDIGFT
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEE
VNGEDTPIIPVMIGDPQKAVQFANGLKEKGIEAPAIRPPTVAEGESRIRLTVTAERRLKD
ECCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHH
IEDLLEGFKLMGRELNLVK
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRIDEWLSSRLAKTKAAGLYRSLKLPQAERTNWASNDYLGLANDKRLIHAAETALRRFGA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCC
GSTGSRLTSGNTAWHEKLERKIAGFKQTEAALLFSSGYLANVGVLSSLPEKGDVILSDQL
CCCCCCCCCCCHHHHHHHHHHHCCCCHHHHEEEEECCCHHHHHHHHCCCCCCCEEEECCC
NHASIIDGCRLSKADTVVYRHKDMNDLEEKLRAAQSRARRFIVTDGVFSMDGTIAPLDEI
CCHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEEECCEEECCCCCCCHHHH
MLLAKQYRAFVIVDDAHATGVLGEAGRGTSEYFGVSPDVVIGTLSKAVGAEGGFVAGSKT
HHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHCCCCCEECCHHH
LIDFLLNHARTFIFQTAVPPASCAAACKALDIIEDSRAKRRLLQSSVNTIKRSLVDIGFT
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEE
VNGEDTPIIPVMIGDPQKAVQFANGLKEKGIEAPAIRPPTVAEGESRIRLTVTAERRLKD
ECCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHH
IEDLLEGFKLMGRELNLVK
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA