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The map label for this gene is ligA

Identifier: 52784516

GI number: 52784516

Start: 720206

End: 722209

Strand: Direct

Name: ligA

Synonym: BLi00716

Alternate gene names: 52784516

Gene position: 720206-722209 (Clockwise)

Preceding gene: 52784515

Following gene: 52784517

Centisome position: 17.06

GC content: 50.95

Gene sequence:

>2004_bases
ATGGAAAAAGAAGCAGCTAAACGCCGTGTGGAACAACTGCATGCATTGATTAACAAATACAACTACGAATATCACACCCT
TGACGATCCAAGCGTGCCTGATTCCGAATATGACAAGCTGATGAAAGAGCTGATTGCTCTTGAAGAAGAGCATCCCGACC
TGAAAACGCCGGACTCTCCTTCTCAGCGCGTCGGCGGAGCCGTTTTGGACGCCTTTCAAAAAGTGCAGCACAAAACGCCG
ATGCTGAGCCTCGGCAACGCGTTTAATGAAGAAGATCTGCGCGACTTTGACCGCCGCGTCCGCCAGGCGGTCGGAGACGT
TGAATACAACGTCGAGTTTAAAATAGACGGTCTTGCTGTTTCACTGCGCTATGAAAACGGCGTATTTGTCAGAGGTGCGA
CGAGAGGCGACGGTACGACGGGCGAGGATATTACGGAAAATTTGAAAACCATCAGAAACATTCCCCTCAGAATGAAACGC
GATCTTTCTATTGAAGTGCGCGGCGAAGCTTTTATGCCGAAGCGCTCCTTTGAACTGCTGAACAAAGCGCGGATCGAACG
TGATGAAGAGCCGTTCGCCAACCCGCGGAACGCCGCTGCCGGTTCATTAAGGCAGCTCGATCCGAAAATTGCCGCGAAAC
GAAATCTCGATATCTTCGTCTACAGTATAGCGGAGCTTGATGAAATGGGCGTTGAAACGCAAAGCCAGGGACTCGATTTC
CTCGACGAACTCGGCTTCAAAACCAATCATGAAAGAAAAAAATGCAGCACGATCGAAGAAGTCATTGAGATTGTCGAAGA
GCTCAAGACAAAACGCGCCGACCTCCCGTATGAAATCGACGGGATCGTCATTAAAGTCGATTCCCTTGACCAGCAGGAAG
AGCTCGGCTTTACGGCGAAAAGCCCGCGCTGGGCGATCGCCTACAAGTTTCCTGCCGAAGAGGTTGTTACGACGCTTTTG
GACATTGAATTAAGCGTCGGCCGGACGGGCGCAGTGACCCCGACTGCGATTCTCGAACCTGTAAAAGTGGCGGGAACGAC
CGTCCAAAGAGCTTCTCTCCACAACGAAGATTTAATTAAAGAGAAGGATATCAGACTGCTGGACAAAGTCGTCGTCAAAA
AGGCGGGAGACATCATTCCGGAGGTCGTCAACGTCCTCGTCGAACAGCGGACGGGCAAAGAAAAAGAATTCAACATGCCG
AAGGAATGCCCGGAATGCGGAAGCGAGCTTGTCAGAATCGAAGGAGAAGTCGCGCTTCGCTGCATTAATCCGGAATGTCC
GGCTCAGATCAGGGAAGGCCTGATCCATTTTGTTTCCCGGAATGCGATGAATATAGATGGTCTCGGCGAGCGCGTCATCA
CCCAGCTGTTCCGCGAAGACCTCGTCCATAATGTCGCCGATCTGTATAAGCTGACGCGCGAGCAGCTGATCAATCTCGAG
CGGATGGGGGAAAAGTCGACCGACAACTTATTGAATTCGATTGAAAAATCGAAGAAGAACTCATTGGAACGGCTTCTCTT
CGGACTCGGCATCCGCTTCATCGGCGCCAAAGCGGCCAAGACGCTGGCGATGCATTTTGAAACGCTCGATAAGCTTAAAA
AAGCGACAAAAGAAGAATTGATCGAAGTCGATGAAATCGGCGACAAGATGGCGGACGCCCTCGTCACCTATTTTGAAAAA
GAAGAGATTCTGAAGCTGTTGGACGAGCTTGAAGAACTCGGGGTCAACACCGTGTATAAAGGCCCGAAAAAAGCGGCCGC
TGAAGCGAGCGATTCGTATTTTGCGGGGAAAACGATCGTCCTGACCGGGAAGCTCAGCGAGATGTCGCGGAATGACGCGA
AAGCGGAAATCGAAGCGCTCGGCGGAAAAATCACAGGCAGTGTAAGCAAAAAAACCGATCTCGTCATTGCCGGCGAAGCG
GCAGGCAGCAAACTGGCGAAAGCTGAAGACCTAAACATCGAAGTATGGGATGAGGCAAGACTGATCAGTGAGCTAAAGAA
ATAA

Upstream 100 bases:

>100_bases
CGCCTTTCCGAGCCCGACCGGAGTGAAGCGTCTTCTCGCGGCCTTTGCGCCAATTGAAAAGCAATAATGGCAAAAGACCT
GAAATGAAAGGACGAAACCA

Downstream 100 bases:

>100_bases
GAGGAGTGTTTTCTATTGAAAAAGATATTATGTTTGGCGGCTGCTGCGGGTATGCTCATGCTCTCAGCCTGTGCGCCGAA
TTTTGGGGGAGAAGAAGAGC

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 667; Mature: 667

Protein sequence:

>667_residues
MEKEAAKRRVEQLHALINKYNYEYHTLDDPSVPDSEYDKLMKELIALEEEHPDLKTPDSPSQRVGGAVLDAFQKVQHKTP
MLSLGNAFNEEDLRDFDRRVRQAVGDVEYNVEFKIDGLAVSLRYENGVFVRGATRGDGTTGEDITENLKTIRNIPLRMKR
DLSIEVRGEAFMPKRSFELLNKARIERDEEPFANPRNAAAGSLRQLDPKIAAKRNLDIFVYSIAELDEMGVETQSQGLDF
LDELGFKTNHERKKCSTIEEVIEIVEELKTKRADLPYEIDGIVIKVDSLDQQEELGFTAKSPRWAIAYKFPAEEVVTTLL
DIELSVGRTGAVTPTAILEPVKVAGTTVQRASLHNEDLIKEKDIRLLDKVVVKKAGDIIPEVVNVLVEQRTGKEKEFNMP
KECPECGSELVRIEGEVALRCINPECPAQIREGLIHFVSRNAMNIDGLGERVITQLFREDLVHNVADLYKLTREQLINLE
RMGEKSTDNLLNSIEKSKKNSLERLLFGLGIRFIGAKAAKTLAMHFETLDKLKKATKEELIEVDEIGDKMADALVTYFEK
EEILKLLDELEELGVNTVYKGPKKAAAEASDSYFAGKTIVLTGKLSEMSRNDAKAEIEALGGKITGSVSKKTDLVIAGEA
AGSKLAKAEDLNIEVWDEARLISELKK

Sequences:

>Translated_667_residues
MEKEAAKRRVEQLHALINKYNYEYHTLDDPSVPDSEYDKLMKELIALEEEHPDLKTPDSPSQRVGGAVLDAFQKVQHKTP
MLSLGNAFNEEDLRDFDRRVRQAVGDVEYNVEFKIDGLAVSLRYENGVFVRGATRGDGTTGEDITENLKTIRNIPLRMKR
DLSIEVRGEAFMPKRSFELLNKARIERDEEPFANPRNAAAGSLRQLDPKIAAKRNLDIFVYSIAELDEMGVETQSQGLDF
LDELGFKTNHERKKCSTIEEVIEIVEELKTKRADLPYEIDGIVIKVDSLDQQEELGFTAKSPRWAIAYKFPAEEVVTTLL
DIELSVGRTGAVTPTAILEPVKVAGTTVQRASLHNEDLIKEKDIRLLDKVVVKKAGDIIPEVVNVLVEQRTGKEKEFNMP
KECPECGSELVRIEGEVALRCINPECPAQIREGLIHFVSRNAMNIDGLGERVITQLFREDLVHNVADLYKLTREQLINLE
RMGEKSTDNLLNSIEKSKKNSLERLLFGLGIRFIGAKAAKTLAMHFETLDKLKKATKEELIEVDEIGDKMADALVTYFEK
EEILKLLDELEELGVNTVYKGPKKAAAEASDSYFAGKTIVLTGKLSEMSRNDAKAEIEALGGKITGSVSKKTDLVIAGEA
AGSKLAKAEDLNIEVWDEARLISELKK
>Mature_667_residues
MEKEAAKRRVEQLHALINKYNYEYHTLDDPSVPDSEYDKLMKELIALEEEHPDLKTPDSPSQRVGGAVLDAFQKVQHKTP
MLSLGNAFNEEDLRDFDRRVRQAVGDVEYNVEFKIDGLAVSLRYENGVFVRGATRGDGTTGEDITENLKTIRNIPLRMKR
DLSIEVRGEAFMPKRSFELLNKARIERDEEPFANPRNAAAGSLRQLDPKIAAKRNLDIFVYSIAELDEMGVETQSQGLDF
LDELGFKTNHERKKCSTIEEVIEIVEELKTKRADLPYEIDGIVIKVDSLDQQEELGFTAKSPRWAIAYKFPAEEVVTTLL
DIELSVGRTGAVTPTAILEPVKVAGTTVQRASLHNEDLIKEKDIRLLDKVVVKKAGDIIPEVVNVLVEQRTGKEKEFNMP
KECPECGSELVRIEGEVALRCINPECPAQIREGLIHFVSRNAMNIDGLGERVITQLFREDLVHNVADLYKLTREQLINLE
RMGEKSTDNLLNSIEKSKKNSLERLLFGLGIRFIGAKAAKTLAMHFETLDKLKKATKEELIEVDEIGDKMADALVTYFEK
EEILKLLDELEELGVNTVYKGPKKAAAEASDSYFAGKTIVLTGKLSEMSRNDAKAEIEALGGKITGSVSKKTDLVIAGEA
AGSKLAKAEDLNIEVWDEARLISELKK

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=669, Percent_Identity=49.3273542600897, Blast_Score=639, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=570, Percent_Identity=22.9824561403509, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_BACLD (Q65MR2)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_077940.1
- RefSeq:   YP_090345.1
- HSSP:   O87703
- ProteinModelPortal:   Q65MR2
- STRING:   Q65MR2
- EnsemblBacteria:   EBBACT00000054283
- EnsemblBacteria:   EBBACT00000061994
- GeneID:   3031245
- GeneID:   3098899
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00716
- KEGG:   bli:BL00589
- NMPDR:   fig|279010.5.peg.1023
- eggNOG:   COG0272
- GeneTree:   EBGT00050000002892
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- BioCyc:   BLIC279010-1:BLI00716-MONOMER
- BioCyc:   BLIC279010:BL00589-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74783; Mature: 74783

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 114-114 BINDING 112-112 BINDING 135-135 BINDING 169-169 BINDING 285-285 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKEAAKRRVEQLHALINKYNYEYHTLDDPSVPDSEYDKLMKELIALEEEHPDLKTPDSP
CCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
SQRVGGAVLDAFQKVQHKTPMLSLGNAFNEEDLRDFDRRVRQAVGDVEYNVEFKIDGLAV
HHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEE
SLRYENGVFVRGATRGDGTTGEDITENLKTIRNIPLRMKRDLSIEVRGEAFMPKRSFELL
EEEECCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHH
NKARIERDEEPFANPRNAAAGSLRQLDPKIAAKRNLDIFVYSIAELDEMGVETQSQGLDF
HHHHCCCCCCCCCCCCCHHHCHHHHCCHHHHHCCCCEEEEEEHHHHHHHCCCCHHCCCHH
LDELGFKTNHERKKCSTIEEVIEIVEELKTKRADLPYEIDGIVIKVDSLDQQEELGFTAK
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEECCCCCHHHCCCCCC
SPRWAIAYKFPAEEVVTTLLDIELSVGRTGAVTPTAILEPVKVAGTTVQRASLHNEDLIK
CCCEEEEEECCHHHHHHHHHHHHEECCCCCCCCHHHHHCCHHHHCCHHHHHHCCCHHHHH
EKDIRLLDKVVVKKAGDIIPEVVNVLVEQRTGKEKEFNMPKECPECGSELVRIEGEVALR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHEEECCEEEEE
CINPECPAQIREGLIHFVSRNAMNIDGLGERVITQLFREDLVHNVADLYKLTREQLINLE
ECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMGEKSTDNLLNSIEKSKKNSLERLLFGLGIRFIGAKAAKTLAMHFETLDKLKKATKEEL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEVDEIGDKMADALVTYFEKEEILKLLDELEELGVNTVYKGPKKAAAEASDSYFAGKTIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEECCCEEE
LTGKLSEMSRNDAKAEIEALGGKITGSVSKKTDLVIAGEAAGSKLAKAEDLNIEVWDEAR
EECCHHHHCCCHHHHHHHHHCCCEECCCCCCCCEEEECCCCCCCCCCCCCCCEEECHHHH
LISELKK
HHHHHCC
>Mature Secondary Structure
MEKEAAKRRVEQLHALINKYNYEYHTLDDPSVPDSEYDKLMKELIALEEEHPDLKTPDSP
CCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
SQRVGGAVLDAFQKVQHKTPMLSLGNAFNEEDLRDFDRRVRQAVGDVEYNVEFKIDGLAV
HHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEE
SLRYENGVFVRGATRGDGTTGEDITENLKTIRNIPLRMKRDLSIEVRGEAFMPKRSFELL
EEEECCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHH
NKARIERDEEPFANPRNAAAGSLRQLDPKIAAKRNLDIFVYSIAELDEMGVETQSQGLDF
HHHHCCCCCCCCCCCCCHHHCHHHHCCHHHHHCCCCEEEEEEHHHHHHHCCCCHHCCCHH
LDELGFKTNHERKKCSTIEEVIEIVEELKTKRADLPYEIDGIVIKVDSLDQQEELGFTAK
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEECCCCCHHHCCCCCC
SPRWAIAYKFPAEEVVTTLLDIELSVGRTGAVTPTAILEPVKVAGTTVQRASLHNEDLIK
CCCEEEEEECCHHHHHHHHHHHHEECCCCCCCCHHHHHCCHHHHCCHHHHHHCCCHHHHH
EKDIRLLDKVVVKKAGDIIPEVVNVLVEQRTGKEKEFNMPKECPECGSELVRIEGEVALR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHEEECCEEEEE
CINPECPAQIREGLIHFVSRNAMNIDGLGERVITQLFREDLVHNVADLYKLTREQLINLE
ECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMGEKSTDNLLNSIEKSKKNSLERLLFGLGIRFIGAKAAKTLAMHFETLDKLKKATKEEL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IEVDEIGDKMADALVTYFEKEEILKLLDELEELGVNTVYKGPKKAAAEASDSYFAGKTIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEECCCEEE
LTGKLSEMSRNDAKAEIEALGGKITGSVSKKTDLVIAGEAAGSKLAKAEDLNIEVWDEAR
EECCHHHHCCCHHHHHHHHHCCCEECCCCCCCCEEEECCCCCCCCCCCCCCCEEECHHHH
LISELKK
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA