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The map label for this gene is pcrA [H]

Identifier: 52784515

GI number: 52784515

Start: 717953

End: 720172

Strand: Direct

Name: pcrA [H]

Synonym: BLi00715

Alternate gene names: 52784515

Gene position: 717953-720172 (Clockwise)

Preceding gene: 52784514

Following gene: 52784516

Centisome position: 17.0

GC content: 50.27

Gene sequence:

>2220_bases
TTGAATTACATTAGCGACCAATTATTAAGCGGATTGAACAAGATGCAGCAGGAAGCGGTCAAAACGACGGACGGTCCGCT
TTTGATCATGGCAGGAGCAGGAAGCGGAAAGACGCGGGTATTGACGCACCGCATTGCGTATTTAATGGCGGAGAAGCGCG
TTGCCCCGTGGAACATACTGGCGATTACATTTACGAACAAAGCGGCCAGAGAAATGAAAGAACGTGTGGAAAGCATTCTC
GGACCGGGAGCGGATGACATCTGGATTTCGACGTTCCACAGCATGTGCGTCAGAATTTTGCGGAGAGACATCGACAGAAT
CGGCATCAACCGGAATTTCTCGATTCTTGATACGTCTGACCAGCTTTCCGTCATTAAAGGAATCCTGAAAGAAAGAAACA
TCGATCCGAAAAAATTCGATCCGAGAAGCATACTAGGATCGATCAGCAGTGCGAAAAACGAGTTGATCGCCCCGGAAGAA
TACGAGAAAACAGCCGGCGGTTTTTTTGAACAAGTCGTCAGCGATGTCTACAAAGATTATCAGAAGAAACTGCGGAAAAA
CCAGTCGCTCGATTTTGACGATTTGATTATGACGACGATTGTCCTGTTTGACCGGGTGCCGGAAGTGCTTGAATACTACC
AGCGGAAATTCCAATACATTCATGTCGATGAGTATCAGGATACGAACAGGGCGCAGTATTTGCTCGTCAAAAAAATGGCG
CAGCGCTTCCAAAACATCTGCGTTGTCGGGGACTCCGACCAGTCGATCTACAGATGGCGCGGAGCGGATATCGCCAACAT
CCTGTCGTTTGAAAAAGACTATCCGAATGCGAGCGTCATTCTGCTCGAACAAAACTACAGATCGACAAAGCGGATATTGA
ACGCGGCCAATGAAGTGATTCAGAACAACTCAAACAGAAAGCCGAAAAACCTCTGGACCGAAAATGACGAAGGGGCGAAA
ATCTCCTACTTCAGAGGCGACAACGAATTTGGCGAAGGCCAATTTGTCGCGGGCAAAATCAGGGAGCTCGTCGGCTCTGG
AAAACGATCCTTCTCAGATATCGCGATTTTGTACCGGACGAACGCCCAGTCGCGGGTCATTGAGGAAACGCTGATGAAGG
CGAACATCAACTACAACATCGTCGGCGGCACGAAGTTCTATGACAGAAAAGAAATCAAGGACATCCTCGCGTATTTGCGC
CTCGTCGCAAACCCTGACGACGATATCAGCTTTGCGCGGATCGTGAACGTCCCGAAGCGCGGCGTCGGTGCGACTTCAGT
CGATAAAATCGCGGCCTATGCCGACATGAACGGTATGTCTTTATTTGAAGCGCTCGGCCAGGTTGACTTTATCGGCCTCA
GTGCTAGAGCGGCCAACGCGCTTGATGAGTTCAGACAGACGATCGAAAACCTGACGAATATGCAGGAATACTTATCCGTC
ACAGAGCTTACGGAAGAAATCCTTGAAAAAACCGAATACCGCGAAATGCTGAAAGCGGAAAAATCCTTGGAGGCGCAGAG
CCGCCTTGAGAATATCGACGAATTTCTCTCCGTAACGAAAAACTTTGAGCAGCAAAGCGAAGACAAATCGCTCGTCGCTT
TTTTGACCGATTTGGCGCTCATCGCCGATATCGATCAGCTTGATCAGAAGGAAGAAGAGACCGGAAATCAAGACGCGGTC
ATCTTAATGACGCTTCATGCTGCAAAAGGACTCGAATTCCCGGTCGTGTTCCTGATGGGGCTTGAAGAAGGAGTGTTCCC
GCACAGCCGCTCCCTGATGGAGGAAGCGGAGATGGAAGAAGAGCGGAGACTCGCGTACGTCGGCATCACGAGGGCTGAGG
AAGAACTTTATTTAACGAACGCGCGGATGCGGACGTTGTTCGGAAGAACGAATATGAACCCGGAATCAAGGTTCATTGCG
GAAATACCGGAGGAATTATTGGACAATCTAAACGAGAAGAAAAAAGATCTGAAAACGCCGTTTGGAAGAAAGCCCGAGCG
GAGAGGCCCCGTTACCCGTCCGGCTGCATCGTACAGCAAAACCGGCGGCGACGGCGTCAATTGGGCAGTCGGCGACAAAG
CCTCCCATAAAAAATGGGGCGTCGGAACCGTTGTCAGCGTCAAGGGATCCGGCGAATCGACAGAGCTTGACATCGCCTTT
CCGAGCCCGACCGGAGTGAAGCGTCTTCTCGCGGCCTTTGCGCCAATTGAAAAGCAATAA

Upstream 100 bases:

>100_bases
TCGAAGAAGCGCTGAAAACGGTACAGGCCGTAAAAGGATGAGCACAACGAACCGGATTGGTTTATAATAGAACATATGTT
TGAATAGGCGGTGAACGGAA

Downstream 100 bases:

>100_bases
TGGCAAAAGACCTGAAATGAAAGGACGAAACCAATGGAAAAAGAAGCAGCTAAACGCCGTGTGGAACAACTGCATGCATT
GATTAACAAATACAACTACG

Product: PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 739; Mature: 739

Protein sequence:

>739_residues
MNYISDQLLSGLNKMQQEAVKTTDGPLLIMAGAGSGKTRVLTHRIAYLMAEKRVAPWNILAITFTNKAAREMKERVESIL
GPGADDIWISTFHSMCVRILRRDIDRIGINRNFSILDTSDQLSVIKGILKERNIDPKKFDPRSILGSISSAKNELIAPEE
YEKTAGGFFEQVVSDVYKDYQKKLRKNQSLDFDDLIMTTIVLFDRVPEVLEYYQRKFQYIHVDEYQDTNRAQYLLVKKMA
QRFQNICVVGDSDQSIYRWRGADIANILSFEKDYPNASVILLEQNYRSTKRILNAANEVIQNNSNRKPKNLWTENDEGAK
ISYFRGDNEFGEGQFVAGKIRELVGSGKRSFSDIAILYRTNAQSRVIEETLMKANINYNIVGGTKFYDRKEIKDILAYLR
LVANPDDDISFARIVNVPKRGVGATSVDKIAAYADMNGMSLFEALGQVDFIGLSARAANALDEFRQTIENLTNMQEYLSV
TELTEEILEKTEYREMLKAEKSLEAQSRLENIDEFLSVTKNFEQQSEDKSLVAFLTDLALIADIDQLDQKEEETGNQDAV
ILMTLHAAKGLEFPVVFLMGLEEGVFPHSRSLMEEAEMEEERRLAYVGITRAEEELYLTNARMRTLFGRTNMNPESRFIA
EIPEELLDNLNEKKKDLKTPFGRKPERRGPVTRPAASYSKTGGDGVNWAVGDKASHKKWGVGTVVSVKGSGESTELDIAF
PSPTGVKRLLAAFAPIEKQ

Sequences:

>Translated_739_residues
MNYISDQLLSGLNKMQQEAVKTTDGPLLIMAGAGSGKTRVLTHRIAYLMAEKRVAPWNILAITFTNKAAREMKERVESIL
GPGADDIWISTFHSMCVRILRRDIDRIGINRNFSILDTSDQLSVIKGILKERNIDPKKFDPRSILGSISSAKNELIAPEE
YEKTAGGFFEQVVSDVYKDYQKKLRKNQSLDFDDLIMTTIVLFDRVPEVLEYYQRKFQYIHVDEYQDTNRAQYLLVKKMA
QRFQNICVVGDSDQSIYRWRGADIANILSFEKDYPNASVILLEQNYRSTKRILNAANEVIQNNSNRKPKNLWTENDEGAK
ISYFRGDNEFGEGQFVAGKIRELVGSGKRSFSDIAILYRTNAQSRVIEETLMKANINYNIVGGTKFYDRKEIKDILAYLR
LVANPDDDISFARIVNVPKRGVGATSVDKIAAYADMNGMSLFEALGQVDFIGLSARAANALDEFRQTIENLTNMQEYLSV
TELTEEILEKTEYREMLKAEKSLEAQSRLENIDEFLSVTKNFEQQSEDKSLVAFLTDLALIADIDQLDQKEEETGNQDAV
ILMTLHAAKGLEFPVVFLMGLEEGVFPHSRSLMEEAEMEEERRLAYVGITRAEEELYLTNARMRTLFGRTNMNPESRFIA
EIPEELLDNLNEKKKDLKTPFGRKPERRGPVTRPAASYSKTGGDGVNWAVGDKASHKKWGVGTVVSVKGSGESTELDIAF
PSPTGVKRLLAAFAPIEKQ
>Mature_739_residues
MNYISDQLLSGLNKMQQEAVKTTDGPLLIMAGAGSGKTRVLTHRIAYLMAEKRVAPWNILAITFTNKAAREMKERVESIL
GPGADDIWISTFHSMCVRILRRDIDRIGINRNFSILDTSDQLSVIKGILKERNIDPKKFDPRSILGSISSAKNELIAPEE
YEKTAGGFFEQVVSDVYKDYQKKLRKNQSLDFDDLIMTTIVLFDRVPEVLEYYQRKFQYIHVDEYQDTNRAQYLLVKKMA
QRFQNICVVGDSDQSIYRWRGADIANILSFEKDYPNASVILLEQNYRSTKRILNAANEVIQNNSNRKPKNLWTENDEGAK
ISYFRGDNEFGEGQFVAGKIRELVGSGKRSFSDIAILYRTNAQSRVIEETLMKANINYNIVGGTKFYDRKEIKDILAYLR
LVANPDDDISFARIVNVPKRGVGATSVDKIAAYADMNGMSLFEALGQVDFIGLSARAANALDEFRQTIENLTNMQEYLSV
TELTEEILEKTEYREMLKAEKSLEAQSRLENIDEFLSVTKNFEQQSEDKSLVAFLTDLALIADIDQLDQKEEETGNQDAV
ILMTLHAAKGLEFPVVFLMGLEEGVFPHSRSLMEEAEMEEERRLAYVGITRAEEELYLTNARMRTLFGRTNMNPESRFIA
EIPEELLDNLNEKKKDLKTPFGRKPERRGPVTRPAASYSKTGGDGVNWAVGDKASHKKWGVGTVVSVKGSGESTELDIAF
PSPTGVKRLLAAFAPIEKQ

Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=738, Percent_Identity=41.0569105691057, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI48994965, Length=654, Percent_Identity=41.131498470948, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1787196, Length=351, Percent_Identity=28.7749287749288, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6322369, Length=747, Percent_Identity=30.5220883534137, Blast_Score=244, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6324477, Length=678, Percent_Identity=24.7787610619469, Blast_Score=134, Evalue=7e-32,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 83637; Mature: 83637

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYISDQLLSGLNKMQQEAVKTTDGPLLIMAGAGSGKTRVLTHRIAYLMAEKRVAPWNIL
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEE
AITFTNKAAREMKERVESILGPGADDIWISTFHSMCVRILRRDIDRIGINRNFSILDTSD
EEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCH
QLSVIKGILKERNIDPKKFDPRSILGSISSAKNELIAPEEYEKTAGGFFEQVVSDVYKDY
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
QKKLRKNQSLDFDDLIMTTIVLFDRVPEVLEYYQRKFQYIHVDEYQDTNRAQYLLVKKMA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHH
QRFQNICVVGDSDQSIYRWRGADIANILSFEKDYPNASVILLEQNYRSTKRILNAANEVI
HHHHCEEEECCCCCCEEEECCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHH
QNNSNRKPKNLWTENDEGAKISYFRGDNEFGEGQFVAGKIRELVGSGKRSFSDIAILYRT
HCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEC
NAQSRVIEETLMKANINYNIVGGTKFYDRKEIKDILAYLRLVANPDDDISFARIVNVPKR
CCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCC
GVGATSVDKIAAYADMNGMSLFEALGQVDFIGLSARAANALDEFRQTIENLTNMQEYLSV
CCCCCHHHHHHHHHCCCHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
TELTEEILEKTEYREMLKAEKSLEAQSRLENIDEFLSVTKNFEQQSEDKSLVAFLTDLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
IADIDQLDQKEEETGNQDAVILMTLHAAKGLEFPVVFLMGLEEGVFPHSRSLMEEAEMEE
HHHHHHHHHHHHHCCCCCEEEEEEEEHHCCCCCHHHHEECCCCCCCCCHHHHHHHHHHHH
ERRLAYVGITRAEEELYLTNARMRTLFGRTNMNPESRFIAEIPEELLDNLNEKKKDLKTP
HHCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
FGRKPERRGPVTRPAASYSKTGGDGVNWAVGDKASHKKWGVGTVVSVKGSGESTELDIAF
CCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEEEECCCCCCEEEEEC
PSPTGVKRLLAAFAPIEKQ
CCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNYISDQLLSGLNKMQQEAVKTTDGPLLIMAGAGSGKTRVLTHRIAYLMAEKRVAPWNIL
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEE
AITFTNKAAREMKERVESILGPGADDIWISTFHSMCVRILRRDIDRIGINRNFSILDTSD
EEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCH
QLSVIKGILKERNIDPKKFDPRSILGSISSAKNELIAPEEYEKTAGGFFEQVVSDVYKDY
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
QKKLRKNQSLDFDDLIMTTIVLFDRVPEVLEYYQRKFQYIHVDEYQDTNRAQYLLVKKMA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHH
QRFQNICVVGDSDQSIYRWRGADIANILSFEKDYPNASVILLEQNYRSTKRILNAANEVI
HHHHCEEEECCCCCCEEEECCCCHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHH
QNNSNRKPKNLWTENDEGAKISYFRGDNEFGEGQFVAGKIRELVGSGKRSFSDIAILYRT
HCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEC
NAQSRVIEETLMKANINYNIVGGTKFYDRKEIKDILAYLRLVANPDDDISFARIVNVPKR
CCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCC
GVGATSVDKIAAYADMNGMSLFEALGQVDFIGLSARAANALDEFRQTIENLTNMQEYLSV
CCCCCHHHHHHHHHCCCHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
TELTEEILEKTEYREMLKAEKSLEAQSRLENIDEFLSVTKNFEQQSEDKSLVAFLTDLAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
IADIDQLDQKEEETGNQDAVILMTLHAAKGLEFPVVFLMGLEEGVFPHSRSLMEEAEMEE
HHHHHHHHHHHHHCCCCCEEEEEEEEHHCCCCCHHHHEECCCCCCCCCHHHHHHHHHHHH
ERRLAYVGITRAEEELYLTNARMRTLFGRTNMNPESRFIAEIPEELLDNLNEKKKDLKTP
HHCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
FGRKPERRGPVTRPAASYSKTGGDGVNWAVGDKASHKKWGVGTVVSVKGSGESTELDIAF
CCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEEEEECCCCCCEEEEEC
PSPTGVKRLLAAFAPIEKQ
CCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377 [H]