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The map label for this gene is bbmA [H]

Identifier: 52784460

GI number: 52784460

Start: 660157

End: 661920

Strand: Direct

Name: bbmA [H]

Synonym: BLi00658

Alternate gene names: 52784460

Gene position: 660157-661920 (Clockwise)

Preceding gene: 52784459

Following gene: 52784461

Centisome position: 15.63

GC content: 48.64

Gene sequence:

>1764_bases
ATGATGGAATATGCAGCGATACATCATCAGCCTTTCAGCTCTGATGCCTATTCTTACAATGGACGGACATTGCACATCAA
GATCCGTACAAAAAAGGATGATGCCGAACACGTCCGCTTGGTTTGGGGCGATCCTTACGAATACACCGGCGGCACATGGA
AAGCGAACGAGCTTGCGATGGCGAAAATTGCCGCAACAAGCACCCATGATTACTGGTTTGCCGAAGTGGCGCCTCCATTC
AGGCGTCTGCAATACGGATTTATCCTGACAGGCGCTGATGATCGAGACACTTTTTACGGAAGCAATGGTGCATGTCCGTT
TGCCGGGAAAGCGGCGGATATAGGCAAACACTGTTTTAAATTTCCGTTTGTTCATGAGGCAGACACGTTTGATGCACCTG
ACTGGGTCAAATCAACCGTCTGGTATCAAATTTTTCCGGAGCGCTTTGCCAGCGGGCGGGAAGATTTGTCTCCGGAAAAC
GCTTTGCCATGGGGAAGCAAAGATCCTGAGGCGCACGATTTTTTCGGAGGGGATTTGCAGGGGATCATGGACAAGCTGGA
CTATTTGGAAGACTTGGGGGTAGGCGGAATCTATTTGACGCCGATCTTTGCCGCGCCTTCCAACCATAAATACGACACAT
TGGACTATTGCTCCATCGATCCGCATTTTGGCGATGAGGAGCTCTTTCGCACGCTGGTCAGCCGGATTCACGAGCGGGGA
ATGAAAATCATGCTTGATGCTGTTTTTAACCACATTGGCAGCGCTTCGCAAGAGTGGCAGGATGTTGTCAAAAACGGTGA
AACGTCCCGCTATAAAGACTGGTTCCATATTCATTCTTTCCCTGTTAAAGAAGGCAGCTATGATACATTTGCGTTTACTC
CCGAGATGCCGAAGCTCAATACGGCCAACCCGGAAGTTCAGGCTTATTTGCTTGATATTGCGCTGTACTGGATCCGCGAA
TTTGATATCGACGGCTGGCGTTTGGATGTGGCAAATGAAGTTGATCATGCGTTTTGGAAGAAATTCCGGCAAGCCGTCAC
GGCAGAAAAGCCCGACATTTTTATATTGGGCGAAATCTGGCATCAGGCTGATCCGTGGCTTAGAGGAGACGAATTTCATT
CGGTCATGAACTACCCGTTCACAGAACCGATGATTGACTATTTTGCAGACGGATCGATTTCAGCTTCGCAAATGGCCAGC
CGCATCAATTCGCATTTAATGAGCGGGATGAAGCAGGTCAATGAGGTGATGTTTAATTTGCTGGACAGTCATGACACGAA
GCGGATTTTGACAAGATGCGGAGGAGATGACAAAAAGGTGCGCTCTCTTTTGGCATTCATGTTTGCTCAGACAGGCTCGC
CGTGCATTTATTACGGAACGGAAGTCGGGCTTGACGGCGGCGATGATCCATTGTGCCGGAAGTGCATGGTTTGGGAAGAG
GAAAAACAGAATCAAGAGATGCTCGCATTTATGAAACGGCTGATCGCTTTGCGAAAACAAGAGAATGATGTATTGACTTA
TGGCGCGCTTGAGTGGAAGCTGGTTGATGACCAAAACGATTTTGTCAGTTTTTCTCGAACGCATGAAGGAAAAGAGCTGA
TCTACTTCTTCCACCAGGGCAGCGAGGTGCGCCGTGTCAGATTGCGGGATTTGAAGATTGCAAGCGATAAAAGAATATAC
GATGCGTGGACGGAAGAAGCGCTTCAGCATGATGATGTGGTCGACATTCAGCCAGGCGGATTTTTCATTTTGGGGGCGGT
CTAA

Upstream 100 bases:

>100_bases
TACCGTTAACAGCTGAAAATGATTGTTCCTGTTACCGCCGTCATGATAATTTCAGAATAAAAGCCGGTTTATCACAGCCG
GACAACCAAAAGGGGGAAAC

Downstream 100 bases:

>100_bases
ATTCTGTTACCGGTAACAGGTTTAAAACTATTGTAACCGCTTTCTGTCGAGGATTACAATTGTCTCAGATTCCAAGATAG
AAGGGGGAAAAAAGATGCAA

Product: maltogenic alpha-amylase

Products: alpha-D-glucose; beta-D-glucose; amylose(n-1) [C]

Alternate protein names: NA

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MMEYAAIHHQPFSSDAYSYNGRTLHIKIRTKKDDAEHVRLVWGDPYEYTGGTWKANELAMAKIAATSTHDYWFAEVAPPF
RRLQYGFILTGADDRDTFYGSNGACPFAGKAADIGKHCFKFPFVHEADTFDAPDWVKSTVWYQIFPERFASGREDLSPEN
ALPWGSKDPEAHDFFGGDLQGIMDKLDYLEDLGVGGIYLTPIFAAPSNHKYDTLDYCSIDPHFGDEELFRTLVSRIHERG
MKIMLDAVFNHIGSASQEWQDVVKNGETSRYKDWFHIHSFPVKEGSYDTFAFTPEMPKLNTANPEVQAYLLDIALYWIRE
FDIDGWRLDVANEVDHAFWKKFRQAVTAEKPDIFILGEIWHQADPWLRGDEFHSVMNYPFTEPMIDYFADGSISASQMAS
RINSHLMSGMKQVNEVMFNLLDSHDTKRILTRCGGDDKKVRSLLAFMFAQTGSPCIYYGTEVGLDGGDDPLCRKCMVWEE
EKQNQEMLAFMKRLIALRKQENDVLTYGALEWKLVDDQNDFVSFSRTHEGKELIYFFHQGSEVRRVRLRDLKIASDKRIY
DAWTEEALQHDDVVDIQPGGFFILGAV

Sequences:

>Translated_587_residues
MMEYAAIHHQPFSSDAYSYNGRTLHIKIRTKKDDAEHVRLVWGDPYEYTGGTWKANELAMAKIAATSTHDYWFAEVAPPF
RRLQYGFILTGADDRDTFYGSNGACPFAGKAADIGKHCFKFPFVHEADTFDAPDWVKSTVWYQIFPERFASGREDLSPEN
ALPWGSKDPEAHDFFGGDLQGIMDKLDYLEDLGVGGIYLTPIFAAPSNHKYDTLDYCSIDPHFGDEELFRTLVSRIHERG
MKIMLDAVFNHIGSASQEWQDVVKNGETSRYKDWFHIHSFPVKEGSYDTFAFTPEMPKLNTANPEVQAYLLDIALYWIRE
FDIDGWRLDVANEVDHAFWKKFRQAVTAEKPDIFILGEIWHQADPWLRGDEFHSVMNYPFTEPMIDYFADGSISASQMAS
RINSHLMSGMKQVNEVMFNLLDSHDTKRILTRCGGDDKKVRSLLAFMFAQTGSPCIYYGTEVGLDGGDDPLCRKCMVWEE
EKQNQEMLAFMKRLIALRKQENDVLTYGALEWKLVDDQNDFVSFSRTHEGKELIYFFHQGSEVRRVRLRDLKIASDKRIY
DAWTEEALQHDDVVDIQPGGFFILGAV
>Mature_587_residues
MMEYAAIHHQPFSSDAYSYNGRTLHIKIRTKKDDAEHVRLVWGDPYEYTGGTWKANELAMAKIAATSTHDYWFAEVAPPF
RRLQYGFILTGADDRDTFYGSNGACPFAGKAADIGKHCFKFPFVHEADTFDAPDWVKSTVWYQIFPERFASGREDLSPEN
ALPWGSKDPEAHDFFGGDLQGIMDKLDYLEDLGVGGIYLTPIFAAPSNHKYDTLDYCSIDPHFGDEELFRTLVSRIHERG
MKIMLDAVFNHIGSASQEWQDVVKNGETSRYKDWFHIHSFPVKEGSYDTFAFTPEMPKLNTANPEVQAYLLDIALYWIRE
FDIDGWRLDVANEVDHAFWKKFRQAVTAEKPDIFILGEIWHQADPWLRGDEFHSVMNYPFTEPMIDYFADGSISASQMAS
RINSHLMSGMKQVNEVMFNLLDSHDTKRILTRCGGDDKKVRSLLAFMFAQTGSPCIYYGTEVGLDGGDDPLCRKCMVWEE
EKQNQEMLAFMKRLIALRKQENDVLTYGALEWKLVDDQNDFVSFSRTHEGKELIYFFHQGSEVRRVRLRDLKIASDKRIY
DAWTEEALQHDDVVDIQPGGFFILGAV

Specific function: Hydrolyzes beta-cyclodextrin to maltose and glucose, soluble starch to maltose and glucose, and pullulan to panose with trace amounts of maltose and glucose. It is also able to hydrolyze acarbose. Can also exhibit a transglycosylation activity transferrin

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. BbmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=397, Percent_Identity=24.9370277078086, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1786604, Length=465, Percent_Identity=32.6881720430108, Blast_Score=218, Evalue=8e-58,
Organism=Escherichia coli, GI1789995, Length=466, Percent_Identity=27.0386266094421, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1790687, Length=394, Percent_Identity=27.9187817258883, Blast_Score=102, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6321726, Length=413, Percent_Identity=23.2445520581114, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321731, Length=403, Percent_Identity=22.3325062034739, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319776, Length=403, Percent_Identity=22.3325062034739, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322245, Length=228, Percent_Identity=26.7543859649123, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324416, Length=279, Percent_Identity=25.4480286738351, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6322241, Length=279, Percent_Identity=25.4480286738351, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6322021, Length=279, Percent_Identity=25.4480286738351, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24583749, Length=421, Percent_Identity=24.7030878859857, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24586587, Length=402, Percent_Identity=24.6268656716418, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24583747, Length=421, Percent_Identity=24.7030878859857, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24586599, Length=420, Percent_Identity=24.5238095238095, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24586589, Length=399, Percent_Identity=25.31328320802, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24583745, Length=230, Percent_Identity=29.1304347826087, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24586591, Length=412, Percent_Identity=25.2427184466019, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI45549022, Length=418, Percent_Identity=24.4019138755981, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24586597, Length=410, Percent_Identity=22.9268292682927, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI221330053, Length=225, Percent_Identity=27.5555555555556, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24586593, Length=399, Percent_Identity=21.5538847117794, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: 3.2.1.20 [C]

Molecular weight: Translated: 67462; Mature: 67462

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEYAAIHHQPFSSDAYSYNGRTLHIKIRTKKDDAEHVRLVWGDPYEYTGGTWKANELAM
CCCCCCCCCCCCCCCCEECCCEEEEEEEEECCCCCCEEEEEECCCCCCCCCCEECCCCEE
AKIAATSTHDYWFAEVAPPFRRLQYGFILTGADDRDTFYGSNGACPFAGKAADIGKHCFK
EEEEECCCCCEEEHHCCCHHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
FPFVHEADTFDAPDWVKSTVWYQIFPERFASGREDLSPENALPWGSKDPEAHDFFGGDLQ
CCCCCCCCCCCCCHHHHHHHHHEECHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
GIMDKLDYLEDLGVGGIYLTPIFAAPSNHKYDTLDYCSIDPHFGDEELFRTLVSRIHERG
HHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCC
MKIMLDAVFNHIGSASQEWQDVVKNGETSRYKDWFHIHSFPVKEGSYDTFAFTPEMPKLN
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCC
TANPEVQAYLLDIALYWIREFDIDGWRLDVANEVDHAFWKKFRQAVTAEKPDIFILGEIW
CCCCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCEEEEEHHH
HQADPWLRGDEFHSVMNYPFTEPMIDYFADGSISASQMASRINSHLMSGMKQVNEVMFNL
HCCCCCCCCHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LDSHDTKRILTRCGGDDKKVRSLLAFMFAQTGSPCIYYGTEVGLDGGDDPLCRKCMVWEE
HCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCEECCCCCCCHHHHHHHCCHH
EKQNQEMLAFMKRLIALRKQENDVLTYGALEWKLVDDQNDFVSFSRTHEGKELIYFFHQG
HHCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCEEECCCCCCCEEEEEEECC
SEVRRVRLRDLKIASDKRIYDAWTEEALQHDDVVDIQPGGFFILGAV
CHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCEEEECCCCEEEEECC
>Mature Secondary Structure
MMEYAAIHHQPFSSDAYSYNGRTLHIKIRTKKDDAEHVRLVWGDPYEYTGGTWKANELAM
CCCCCCCCCCCCCCCCEECCCEEEEEEEEECCCCCCEEEEEECCCCCCCCCCEECCCCEE
AKIAATSTHDYWFAEVAPPFRRLQYGFILTGADDRDTFYGSNGACPFAGKAADIGKHCFK
EEEEECCCCCEEEHHCCCHHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
FPFVHEADTFDAPDWVKSTVWYQIFPERFASGREDLSPENALPWGSKDPEAHDFFGGDLQ
CCCCCCCCCCCCCHHHHHHHHHEECHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
GIMDKLDYLEDLGVGGIYLTPIFAAPSNHKYDTLDYCSIDPHFGDEELFRTLVSRIHERG
HHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCC
MKIMLDAVFNHIGSASQEWQDVVKNGETSRYKDWFHIHSFPVKEGSYDTFAFTPEMPKLN
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCC
TANPEVQAYLLDIALYWIREFDIDGWRLDVANEVDHAFWKKFRQAVTAEKPDIFILGEIW
CCCCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCEEEEEHHH
HQADPWLRGDEFHSVMNYPFTEPMIDYFADGSISASQMASRINSHLMSGMKQVNEVMFNL
HCCCCCCCCHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LDSHDTKRILTRCGGDDKKVRSLLAFMFAQTGSPCIYYGTEVGLDGGDDPLCRKCMVWEE
HCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEECCEECCCCCCCHHHHHHHCCHH
EKQNQEMLAFMKRLIALRKQENDVLTYGALEWKLVDDQNDFVSFSRTHEGKELIYFFHQG
HHCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCEEECCCCCCCEEEEEEECC
SEVRRVRLRDLKIASDKRIYDAWTEEALQHDDVVDIQPGGFFILGAV
CHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCEEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): {} [C]

Substrates: Maltose; H2O; 1,4-alpha-D-glucan [C]

Specific reaction: Maltose + H2O = 2 alpha-D-glucose H2O + 1,4-alpha-D-glucan = beta-D-glucose + amylose(n-1) [C]

General reaction: O-Glycosyl bond hydrolysis; Exohydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]