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The map label for this gene is sigB [H]

Identifier: 52784380

GI number: 52784380

Start: 563393

End: 564187

Strand: Direct

Name: sigB [H]

Synonym: BLi00560

Alternate gene names: 52784380

Gene position: 563393-564187 (Clockwise)

Preceding gene: 52784379

Following gene: 52784381

Centisome position: 13.34

GC content: 47.04

Gene sequence:

>795_bases
ATGATGGCACAACCGTCCAAAACTACGAAACTAACTAAAACCGAAGTCGACACGCTGATTAAAAAATATCAGGAGAACCA
GGATGAAGACGCTCAGCTGACCCTTGTTCAAACATATAAAAATCTGGTGGAAACACTCGCCAAAAAATATTCAAAAGGAA
AAAGCTTTCACGAAGATCTATGCCAGGTTGGGATGCTCGGATTGCTTGGAGCCATCAAACGCTATGACCCCGATGTGGGA
AAATCGTTTGAAGCGTTTGCCATTCCGACCATCATCGGAGAAATCAAACGGTTTCTCCGAGATAAGACCTGGAGCGTCCA
CGTTCCGAGAAGGATCAAGGAGCTCGGGCCGAGAATCAAAATGGCGGTGGATCATTTAACGACTGAAACGCAAAGGTCTC
CAAGAGTCGATGAGATTGCCGAGTTTCTTGACGTTACAGAAGAAGAAGTGCTGGAAACGATGGAGATGGGCAAAAGCTAT
CAGGCGCTTTCCGTCGACCACAGCATTGAAGCTGATGCAGACGGCAGCACGGTGACGATCTTGGACATCGTCGGCTCGCA
GGAGGAAGGCTATGAAAAGGTGAACCAAAAGCTGATGCTTGAAAGCGTATTACACGTACTATCCGACCGTGAAAAGCAAA
TCATTGAGCTGACTTACATTCAAAACAAAAGCCAAAAAGAAACCGGCGAAATGCTGGGCATATCGCAAATGCACGTCTCA
CGGCTGCAGCGCAAAGCTGTCAAAAGGCTGAGGGAAGCATTGGCTGAAGATCCGTATATGGAGCTTAAACCATGA

Upstream 100 bases:

>100_bases
GGTTTATATTTAATGGAGACGCTCATGGATGAAGTGCATGTCCAGATTGACTCAGGCGTAACCGTCTCGATGACAAAGTT
CTTAAACAGGGAGCGAGTTG

Downstream 100 bases:

>100_bases
TTTGGACTGAGTCGAATGAACACATGCAAGCGCTTGTTTATCAGCTTCCTAAAGAAGGAAAAGCCGTCTGCGGTGACAGT
TTTTATTTCCATGCGACTGA

Product: RNA polymerase sigma factor SigB

Products: NA

Alternate protein names: General stress protein 84; GSP84; Sigma-37 [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MMAQPSKTTKLTKTEVDTLIKKYQENQDEDAQLTLVQTYKNLVETLAKKYSKGKSFHEDLCQVGMLGLLGAIKRYDPDVG
KSFEAFAIPTIIGEIKRFLRDKTWSVHVPRRIKELGPRIKMAVDHLTTETQRSPRVDEIAEFLDVTEEEVLETMEMGKSY
QALSVDHSIEADADGSTVTILDIVGSQEEGYEKVNQKLMLESVLHVLSDREKQIIELTYIQNKSQKETGEMLGISQMHVS
RLQRKAVKRLREALAEDPYMELKP

Sequences:

>Translated_264_residues
MMAQPSKTTKLTKTEVDTLIKKYQENQDEDAQLTLVQTYKNLVETLAKKYSKGKSFHEDLCQVGMLGLLGAIKRYDPDVG
KSFEAFAIPTIIGEIKRFLRDKTWSVHVPRRIKELGPRIKMAVDHLTTETQRSPRVDEIAEFLDVTEEEVLETMEMGKSY
QALSVDHSIEADADGSTVTILDIVGSQEEGYEKVNQKLMLESVLHVLSDREKQIIELTYIQNKSQKETGEMLGISQMHVS
RLQRKAVKRLREALAEDPYMELKP
>Mature_264_residues
MMAQPSKTTKLTKTEVDTLIKKYQENQDEDAQLTLVQTYKNLVETLAKKYSKGKSFHEDLCQVGMLGLLGAIKRYDPDVG
KSFEAFAIPTIIGEIKRFLRDKTWSVHVPRRIKELGPRIKMAVDHLTTETQRSPRVDEIAEFLDVTEEEVLETMEMGKSY
QALSVDHSIEADADGSTVTILDIVGSQEEGYEKVNQKLMLESVLHVLSDREKQIIELTYIQNKSQKETGEMLGISQMHVS
RLQRKAVKRLREALAEDPYMELKP

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma B is not essential for sporulation; rather it is required for maximal expression of ctc and csbA which are transcr

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=204, Percent_Identity=28.921568627451, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789098, Length=229, Percent_Identity=24.8908296943231, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014288
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 30131; Mature: 30131

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAQPSKTTKLTKTEVDTLIKKYQENQDEDAQLTLVQTYKNLVETLAKKYSKGKSFHEDL
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
CQVGMLGLLGAIKRYDPDVGKSFEAFAIPTIIGEIKRFLRDKTWSVHVPRRIKELGPRIK
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHCCHHH
MAVDHLTTETQRSPRVDEIAEFLDVTEEEVLETMEMGKSYQALSVDHSIEADADGSTVTI
HHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCEEEE
LDIVGSQEEGYEKVNQKLMLESVLHVLSDREKQIIELTYIQNKSQKETGEMLGISQMHVS
EEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RLQRKAVKRLREALAEDPYMELKP
HHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMAQPSKTTKLTKTEVDTLIKKYQENQDEDAQLTLVQTYKNLVETLAKKYSKGKSFHEDL
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
CQVGMLGLLGAIKRYDPDVGKSFEAFAIPTIIGEIKRFLRDKTWSVHVPRRIKELGPRIK
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHCCHHH
MAVDHLTTETQRSPRVDEIAEFLDVTEEEVLETMEMGKSYQALSVDHSIEADADGSTVTI
HHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCEEEE
LDIVGSQEEGYEKVNQKLMLESVLHVLSDREKQIIELTYIQNKSQKETGEMLGISQMHVS
EEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RLQRKAVKRLREALAEDPYMELKP
HHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3016731; 3027048; 2170324; 9384377; 9298659 [H]