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The map label for this gene is rsbX [H]

Identifier: 52784381

GI number: 52784381

Start: 564184

End: 564783

Strand: Direct

Name: rsbX [H]

Synonym: BLi00561

Alternate gene names: 52784381

Gene position: 564184-564783 (Clockwise)

Preceding gene: 52784380

Following gene: 52784382

Centisome position: 13.36

GC content: 45.67

Gene sequence:

>600_bases
ATGATTTGGACTGAGTCGAATGAACACATGCAAGCGCTTGTTTATCAGCTTCCTAAAGAAGGAAAAGCCGTCTGCGGTGA
CAGTTTTTATTTCCATGCGACTGACGAAACCATGGTGTGCGCACTGGCTGACGGGCTTGGCAGCGGTGCGTTGGCACGCG
AATCCTCTTCGGTCATCAGCGAAGTTGTCGAAGAGCACGGCCATGAAGATGTCGAAAGCTTGATGGAACGGTGCAATCAA
GCGCTGAAACATAAGCGCGGTGCGACCGTTTCAATTTTAAAAGTCGATTTTCGAACAAGGACGATCGCTTACAGCTCTGT
AGGCAATATTCGCTTTATCTTATACGCTCCTTCGGACAAATATATCCACCCTCTCCCTGTGACGGGATACATGTCGGGAA
AACCGCAAACATACAAAACATTCACCTATACGTATGAAAAAGGATCAAGATTCATCATTTATTCAGACGGTTTGGAAGTA
CCCGCCCTCCGGACTTATTTAAAACAGCACCATACACCGGGAGATATATCGAATCAGCTTGAATCATACACACGAATGGG
AAAAGACGATCTCACCTATATCATTGGACAGCTTTTTTAA

Upstream 100 bases:

>100_bases
TGCTGGGCATATCGCAAATGCACGTCTCACGGCTGCAGCGCAAAGCTGTCAAAAGGCTGAGGGAAGCATTGGCTGAAGAT
CCGTATATGGAGCTTAAACC

Downstream 100 bases:

>100_bases
GGAAGCTGCCCTTTGATATAGGTGTTTTTCCGTTTCTTTTGGTACACTAAGAAGACAACATTCTTTTGGAGGCTACAATG
GATACTTTAGCGTCAATCGT

Product: RsbX

Products: NA

Alternate protein names: Sigma-B negative effector [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MIWTESNEHMQALVYQLPKEGKAVCGDSFYFHATDETMVCALADGLGSGALARESSSVISEVVEEHGHEDVESLMERCNQ
ALKHKRGATVSILKVDFRTRTIAYSSVGNIRFILYAPSDKYIHPLPVTGYMSGKPQTYKTFTYTYEKGSRFIIYSDGLEV
PALRTYLKQHHTPGDISNQLESYTRMGKDDLTYIIGQLF

Sequences:

>Translated_199_residues
MIWTESNEHMQALVYQLPKEGKAVCGDSFYFHATDETMVCALADGLGSGALARESSSVISEVVEEHGHEDVESLMERCNQ
ALKHKRGATVSILKVDFRTRTIAYSSVGNIRFILYAPSDKYIHPLPVTGYMSGKPQTYKTFTYTYEKGSRFIIYSDGLEV
PALRTYLKQHHTPGDISNQLESYTRMGKDDLTYIIGQLF
>Mature_199_residues
MIWTESNEHMQALVYQLPKEGKAVCGDSFYFHATDETMVCALADGLGSGALARESSSVISEVVEEHGHEDVESLMERCNQ
ALKHKRGATVSILKVDFRTRTIAYSSVGNIRFILYAPSDKYIHPLPVTGYMSGKPQTYKTFTYTYEKGSRFIIYSDGLEV
PALRTYLKQHHTPGDISNQLESYTRMGKDDLTYIIGQLF

Specific function: Negative regulator of sigma-B activity. Dephosphorylates rsbS. Plays a role both in maintaining low sigma-B activity during growth and in reestablishing prestress sigma-B activity after induction. Could have a negative feedback role by indirectly communic

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001932
- InterPro:   IPR010822 [H]

Pfam domain/function: PF07228 SpoIIE [H]

EC number: =3.1.3.3 [H]

Molecular weight: Translated: 22421; Mature: 22421

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWTESNEHMQALVYQLPKEGKAVCGDSFYFHATDETMVCALADGLGSGALARESSSVIS
CCCCCCCHHHHHHHHHCCCCCCEEECCCEEEEECCCEEEEEEECCCCCCCHHHHHHHHHH
EVVEEHGHEDVESLMERCNQALKHKRGATVSILKVDFRTRTIAYSSVGNIRFILYAPSDK
HHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECEEEEEEEECCCCEEEEEECCCCC
YIHPLPVTGYMSGKPQTYKTFTYTYEKGSRFIIYSDGLEVPALRTYLKQHHTPGDISNQL
CCCCCCCCCEECCCCCEEEEEEEEEECCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHH
ESYTRMGKDDLTYIIGQLF
HHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MIWTESNEHMQALVYQLPKEGKAVCGDSFYFHATDETMVCALADGLGSGALARESSSVIS
CCCCCCCHHHHHHHHHCCCCCCEEECCCEEEEECCCEEEEEEECCCCCCCHHHHHHHHHH
EVVEEHGHEDVESLMERCNQALKHKRGATVSILKVDFRTRTIAYSSVGNIRFILYAPSDK
HHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECEEEEEEEECCCCEEEEEECCCCC
YIHPLPVTGYMSGKPQTYKTFTYTYEKGSRFIIYSDGLEVPALRTYLKQHHTPGDISNQL
CCCCCCCCCEECCCCCEEEEEEEEEECCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHH
ESYTRMGKDDLTYIIGQLF
HHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2170324; 9384377; 3027048; 1592822; 8468294; 8824586; 9658013 [H]