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The map label for this gene is rsbW

Identifier: 52784379

GI number: 52784379

Start: 562945

End: 563430

Strand: Direct

Name: rsbW

Synonym: BLi00559

Alternate gene names: 52784379

Gene position: 562945-563430 (Clockwise)

Preceding gene: 52784378

Following gene: 52784380

Centisome position: 13.33

GC content: 46.5

Gene sequence:

>486_bases
ATGAAGAGAGCAGCTGATTACATTGAAATGAAGTCACCTGCCAAACCAGAATACGTAGGAATCATTCGTTTAACCCTGTC
GGGGATTGCAAGCAAGATGGGGTATTCTTATGATGACATCGAAGACTTGAAAATTGCGGTCAGCGAAGCGTGCACAAACG
CTGTGCAGCATGCTTATAAAGCAGACAACAACACAGGAGAGGTCTCCGTCAGATTCGGCGTGTTCGAAGACCGCCTCGAA
ATCGTCGTCGCCGATCAGGGAGACAGTTTTGATATTCAGGATAAACAGAAGGAGCTCGGACCATATTCCCCTGAGCATAC
GGTAGATCAATTGTCAGAAGGAGGGCTCGGTTTATATTTAATGGAGACGCTCATGGATGAAGTGCATGTCCAGATTGACT
CAGGCGTAACCGTCTCGATGACAAAGTTCTTAAACAGGGAGCGAGTTGATGATGGCACAACCGTCCAAAACTACGAAACT
AACTAA

Upstream 100 bases:

>100_bases
TAGTGCTTGAAAATCTGTCAGAGCGTTTGATTCGTTTGTTTGATATTACAGGTTTAAAGGACATCATTGACATTTCTGGA
AAGTCAGAGGGTGGATTATA

Downstream 100 bases:

>100_bases
AACCGAAGTCGACACGCTGATTAAAAAATATCAGGAGAACCAGGATGAAGACGCTCAGCTGACCCTTGTTCAAACATATA
AAAATCTGGTGGAAACACTC

Product: serine-protein kinase RsbW

Products: NA

Alternate protein names: Anti-sigma-B factor; Sigma-B negative effector rsbW

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MKRAADYIEMKSPAKPEYVGIIRLTLSGIASKMGYSYDDIEDLKIAVSEACTNAVQHAYKADNNTGEVSVRFGVFEDRLE
IVVADQGDSFDIQDKQKELGPYSPEHTVDQLSEGGLGLYLMETLMDEVHVQIDSGVTVSMTKFLNRERVDDGTTVQNYET
N

Sequences:

>Translated_161_residues
MKRAADYIEMKSPAKPEYVGIIRLTLSGIASKMGYSYDDIEDLKIAVSEACTNAVQHAYKADNNTGEVSVRFGVFEDRLE
IVVADQGDSFDIQDKQKELGPYSPEHTVDQLSEGGLGLYLMETLMDEVHVQIDSGVTVSMTKFLNRERVDDGTTVQNYET
N
>Mature_161_residues
MKRAADYIEMKSPAKPEYVGIIRLTLSGIASKMGYSYDDIEDLKIAVSEACTNAVQHAYKADNNTGEVSVRFGVFEDRLE
IVVADQGDSFDIQDKQKELGPYSPEHTVDQLSEGGLGLYLMETLMDEVHVQIDSGVTVSMTKFLNRERVDDGTTVQNYET
N

Specific function: Negative regulator of sigma-B activity. Phosphorylates and inactivates its specific antagonist protein, rsbV. Upon phosphorylation of rsbV, rsbW is released and binds to sigma-B, thereby blocking its ability to form an RNA polymerase holoenzyme (E-sigma-B

COG id: COG2172

COG function: function code T; Anti-sigma regulatory factor (Ser/Thr protein kinase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anti-sigma-factor family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSBW_BACLD (Q65N49)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_077798.1
- RefSeq:   YP_090208.1
- ProteinModelPortal:   Q65N49
- SMR:   Q65N49
- STRING:   Q65N49
- EnsemblBacteria:   EBBACT00000056107
- EnsemblBacteria:   EBBACT00000062104
- GeneID:   3030460
- GeneID:   3098519
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00559
- KEGG:   bli:BL02207
- NMPDR:   fig|279010.5.peg.692
- eggNOG:   COG2172
- GeneTree:   EBGT00050000001549
- HOGENOM:   HBG425195
- OMA:   GEITIVF
- ProtClustDB:   PRK04069
- BioCyc:   BLIC279010-1:BLI00559-MONOMER
- BioCyc:   BLIC279010:BL02207-MONOMER
- HAMAP:   MF_00638
- InterPro:   IPR010193
- InterPro:   IPR003594
- Gene3D:   G3DSA:3.30.565.10
- SMART:   SM00387
- TIGRFAMs:   TIGR01924

Pfam domain/function: PF02518 HATPase_c; SSF55874 ATP_bd_ATPase

EC number: =2.7.11.1

Molecular weight: Translated: 17923; Mature: 17923

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRAADYIEMKSPAKPEYVGIIRLTLSGIASKMGYSYDDIEDLKIAVSEACTNAVQHAYK
CCCCCCHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
ADNNTGEVSVRFGVFEDRLEIVVADQGDSFDIQDKQKELGPYSPEHTVDQLSEGGLGLYL
CCCCCCEEEEEECEEECCEEEEEECCCCCCCCCCHHHHCCCCCCCHHHHHHHCCCCHHHH
METLMDEVHVQIDSGVTVSMTKFLNRERVDDGTTVQNYETN
HHHHHHHHEEEECCCCEEHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKRAADYIEMKSPAKPEYVGIIRLTLSGIASKMGYSYDDIEDLKIAVSEACTNAVQHAYK
CCCCCCHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
ADNNTGEVSVRFGVFEDRLEIVVADQGDSFDIQDKQKELGPYSPEHTVDQLSEGGLGLYL
CCCCCCEEEEEECEEECCEEEEEECCCCCCCCCCHHHHCCCCCCCHHHHHHHCCCCHHHH
METLMDEVHVQIDSGVTVSMTKFLNRERVDDGTTVQNYETN
HHHHHHHHEEEECCCCEEHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA