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The map label for this gene is nasB [H]

Identifier: 52784302

GI number: 52784302

Start: 485200

End: 487494

Strand: Direct

Name: nasB [H]

Synonym: BLi00482

Alternate gene names: 52784302

Gene position: 485200-487494 (Clockwise)

Preceding gene: 52784298

Following gene: 52784303

Centisome position: 11.49

GC content: 51.72

Gene sequence:

>2295_bases
ATGGGGAAACAAAGGCTTGTTTTAATAGGAAACGGCATGGCAGGGGTTCGCACGGCCGAAGAGATTTCACTACACGGCGG
GAGCCGGTTTGAGATCGTCATCATCGGCAGCGAACCACACCCGGGCTATAACCGGATTCTGCTGTCTTCTGTCCTTCAGG
GAGAAACGGATTGGAACGATGTGATGACAAAAAGCCGGAGCTGGTATGAAGAAAACGGGATTACCCTTTATACAGGGGAA
ACGGCTGTCGCAATCGATACGGTTAATCAAACGGTGGCGACAGATCAAAACCGCGAAATCGCTTATGACAAACTGATCAT
TGCGACGGGCTCTTCCCCGTTTATCCTCCCGGTTCATGGTGCAGACAAAGAAGGGGTATATGGTTTTAGAACAATAGACG
ATTGCCGGGCATTCATAAACGCATCAAAGCGATTTAAAAAAGCGGCCGTCATCGGCGGGGGAATATTGGGCCTTGAGGCG
GCGCGGGGATTGGCAAACCTCGGGATGAAGGTTGATGTGGTTCACCACTCTTCCTCCATCATGCAGACTCAGCTTGATCC
GCCGGCTTCTGCCATGCTCCAGAAAGAGCTGGAGCGTCAAGGGATCCATTTTCTTTTGGAGAAAGATACAGAGGCGATTT
TAGGAACATCGCGCGCCGAGGGTGTGCGGTTTAAAGACGGGACGAAGATCAGCGCCGATCTCGTCGTCATGGCTGCCGGT
GTCAGACCGAATATCGAACTGGCCAAGGCGAGCGGTATTTCAACCAACAGGGCGATCATCGTCAGCGACTATATGGAGAC
AAATGTTCCAAATGTCTACGCCGTCGGTGAATGCGCGGAGCATAACGGAACGGTCTACGGCTTAGTCAAGCCACTGTATG
AGCAGGGGAAAGTGCTGGCCAAACATATTTGCGGCCTCGAATGTTCCGGTTATCAGGGCTCCGTTCAGTCCGCGGCTTTA
AAAATGTCCGGAATCGACGTCTTTTCGGCAGGGAAAATCACAGAGGACGACTCGACGACGGCTATTAAACTTTTAGACGA
AGCGGCCGGAGTCTATAAAAAAGCGGTCTTTCAAGGGGATAAAATGGCCGGCGTCATTTTATACGGAGACACCAGCGGCA
GCCAAAGGCTGTTGGAAAGCATCATCAAACAAAGGGATATTACCGTTGTCAAAAAAGAGCTGTTTCAATCGGAGGAAGAC
AGTTCAGTCGCTTCTATGGCGGTTCGTGAAACGATCTGCCAATGCAACGCGGTTTCAAAAGGCATGATCATGGAAGCGAT
ACAGACGCACGGTTTAAAGACGGCCGAGGAGGTCAAACGCTGTACAAAAGCTTCCGGGTCATGCGGCGGCTGCAGGCCGT
TGGTCGAGGAACTGCTCCTCCATACCGCCGAACATGATGGTCACATATCTGCGGCGGAACAACCGATGTGCGCCTGCACT
TCATTCACGGAAGATGAAGTCGTGAACGAAATACAGATGAGAAATCTTTCTTCCGTTCACGAAGTCATCTCGGCGCTCGG
CTGGAAAAATAGCAGCGGATGCCGGATTTGCGTGCCCGCGCTTCACTACTATTTAAAAATGATCCGTCCGGACATCGCCT
ATGAGGAGCACGAAGAGGAGACGGATGCCATCATCCCGCAAATGTACGGAGGCCGGACGAATGCCGAAGAGCTGAAGCGC
ATTGCAGGAGTGATTGAAAAATATCAAATTCAAGAGGTGTACATGACACACCATCAGAGATTAAAGCTTGCCGGCATCAA
GCCGGAGCATATAGAGCGCGTGAAAGAAGAACTCGGCATGCCGTATTGCCTGCCGCAACAGCAGCGGATCGCCGCGGTTA
AAACGTGCCCATGCGGATCACCGCAGATTCAAACATTGGCAGCAGACTTGGAAAAAGCAGCAGAATCCCTCCTCATTCCG
GCCAAGGTCTCCATTGGGGTGTCGGGCTGTTTGGACGATTGCGTGTACGCTTCAGTTCATGACATCGGACTTCTCAAAGT
AAACGGAGGATGGGAGATTTATGCGGGCGGCCAAGGAGGACAGCATGCTTCCCCGGGAGAGCTGCTTTCAGTAGCCGACT
CGGCGGAGGAAGCGGGTGAACTCATGAAAGGCCTGCTTCAATATTACCGCGAAACAGCAAACTATCTTGAGAAGATCGGG
CATTGGATTGAACGCGCCGGCATCATTCATATCCGGGAAGTTTTATTTGACAACGGACTTCGCGGCCAGCTGTTCGAACG
GCTTGAAAAAGAAAAGCGGCTGGCTCAGCAAGAAATGATAAAAGGGCTGATGTGA

Upstream 100 bases:

>100_bases
TTCTAAAATTTTTTGTCAGGAAATGTAACAGGTTTGCACGCTTTAGCGATGCAATGTGAAATAATAATCCGGAGCAAAGC
TGCAGAGGGGAGGGGAGAAA

Downstream 100 bases:

>100_bases
AATGACAGAACGATTGCTAAAGTACTTTCGCACAAAGCAAAAAGAAGTGCAATCCGAAAAAACGTATGACACCCAATGTC
CATTTTGCAGCATGCAGTGC

Product: NasB

Products: nitrite; NADH; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 764; Mature: 763

Protein sequence:

>764_residues
MGKQRLVLIGNGMAGVRTAEEISLHGGSRFEIVIIGSEPHPGYNRILLSSVLQGETDWNDVMTKSRSWYEENGITLYTGE
TAVAIDTVNQTVATDQNREIAYDKLIIATGSSPFILPVHGADKEGVYGFRTIDDCRAFINASKRFKKAAVIGGGILGLEA
ARGLANLGMKVDVVHHSSSIMQTQLDPPASAMLQKELERQGIHFLLEKDTEAILGTSRAEGVRFKDGTKISADLVVMAAG
VRPNIELAKASGISTNRAIIVSDYMETNVPNVYAVGECAEHNGTVYGLVKPLYEQGKVLAKHICGLECSGYQGSVQSAAL
KMSGIDVFSAGKITEDDSTTAIKLLDEAAGVYKKAVFQGDKMAGVILYGDTSGSQRLLESIIKQRDITVVKKELFQSEED
SSVASMAVRETICQCNAVSKGMIMEAIQTHGLKTAEEVKRCTKASGSCGGCRPLVEELLLHTAEHDGHISAAEQPMCACT
SFTEDEVVNEIQMRNLSSVHEVISALGWKNSSGCRICVPALHYYLKMIRPDIAYEEHEEETDAIIPQMYGGRTNAEELKR
IAGVIEKYQIQEVYMTHHQRLKLAGIKPEHIERVKEELGMPYCLPQQQRIAAVKTCPCGSPQIQTLAADLEKAAESLLIP
AKVSIGVSGCLDDCVYASVHDIGLLKVNGGWEIYAGGQGGQHASPGELLSVADSAEEAGELMKGLLQYYRETANYLEKIG
HWIERAGIIHIREVLFDNGLRGQLFERLEKEKRLAQQEMIKGLM

Sequences:

>Translated_764_residues
MGKQRLVLIGNGMAGVRTAEEISLHGGSRFEIVIIGSEPHPGYNRILLSSVLQGETDWNDVMTKSRSWYEENGITLYTGE
TAVAIDTVNQTVATDQNREIAYDKLIIATGSSPFILPVHGADKEGVYGFRTIDDCRAFINASKRFKKAAVIGGGILGLEA
ARGLANLGMKVDVVHHSSSIMQTQLDPPASAMLQKELERQGIHFLLEKDTEAILGTSRAEGVRFKDGTKISADLVVMAAG
VRPNIELAKASGISTNRAIIVSDYMETNVPNVYAVGECAEHNGTVYGLVKPLYEQGKVLAKHICGLECSGYQGSVQSAAL
KMSGIDVFSAGKITEDDSTTAIKLLDEAAGVYKKAVFQGDKMAGVILYGDTSGSQRLLESIIKQRDITVVKKELFQSEED
SSVASMAVRETICQCNAVSKGMIMEAIQTHGLKTAEEVKRCTKASGSCGGCRPLVEELLLHTAEHDGHISAAEQPMCACT
SFTEDEVVNEIQMRNLSSVHEVISALGWKNSSGCRICVPALHYYLKMIRPDIAYEEHEEETDAIIPQMYGGRTNAEELKR
IAGVIEKYQIQEVYMTHHQRLKLAGIKPEHIERVKEELGMPYCLPQQQRIAAVKTCPCGSPQIQTLAADLEKAAESLLIP
AKVSIGVSGCLDDCVYASVHDIGLLKVNGGWEIYAGGQGGQHASPGELLSVADSAEEAGELMKGLLQYYRETANYLEKIG
HWIERAGIIHIREVLFDNGLRGQLFERLEKEKRLAQQEMIKGLM
>Mature_763_residues
GKQRLVLIGNGMAGVRTAEEISLHGGSRFEIVIIGSEPHPGYNRILLSSVLQGETDWNDVMTKSRSWYEENGITLYTGET
AVAIDTVNQTVATDQNREIAYDKLIIATGSSPFILPVHGADKEGVYGFRTIDDCRAFINASKRFKKAAVIGGGILGLEAA
RGLANLGMKVDVVHHSSSIMQTQLDPPASAMLQKELERQGIHFLLEKDTEAILGTSRAEGVRFKDGTKISADLVVMAAGV
RPNIELAKASGISTNRAIIVSDYMETNVPNVYAVGECAEHNGTVYGLVKPLYEQGKVLAKHICGLECSGYQGSVQSAALK
MSGIDVFSAGKITEDDSTTAIKLLDEAAGVYKKAVFQGDKMAGVILYGDTSGSQRLLESIIKQRDITVVKKELFQSEEDS
SVASMAVRETICQCNAVSKGMIMEAIQTHGLKTAEEVKRCTKASGSCGGCRPLVEELLLHTAEHDGHISAAEQPMCACTS
FTEDEVVNEIQMRNLSSVHEVISALGWKNSSGCRICVPALHYYLKMIRPDIAYEEHEEETDAIIPQMYGGRTNAEELKRI
AGVIEKYQIQEVYMTHHQRLKLAGIKPEHIERVKEELGMPYCLPQQQRIAAVKTCPCGSPQIQTLAADLEKAAESLLIPA
KVSIGVSGCLDDCVYASVHDIGLLKVNGGWEIYAGGQGGQHASPGELLSVADSAEEAGELMKGLLQYYRETANYLEKIGH
WIERAGIIHIREVLFDNGLRGQLFERLEKEKRLAQQEMIKGLM

Specific function: Required for nitrate assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI65787454, Length=275, Percent_Identity=28, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI226437568, Length=275, Percent_Identity=28, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI21389617, Length=275, Percent_Identity=28, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI4757732, Length=287, Percent_Identity=27.8745644599303, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI22202629, Length=257, Percent_Identity=28.4046692607004, Blast_Score=84, Evalue=5e-16,
Organism=Escherichia coli, GI1789765, Length=794, Percent_Identity=28.9672544080605, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI1789065, Length=301, Percent_Identity=28.2392026578073, Blast_Score=141, Evalue=2e-34,
Organism=Escherichia coli, GI1788892, Length=279, Percent_Identity=23.6559139784946, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787352, Length=225, Percent_Identity=28.4444444444444, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17559934, Length=418, Percent_Identity=24.1626794258373, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI32564386, Length=362, Percent_Identity=24.8618784530387, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24639257, Length=313, Percent_Identity=24.6006389776358, Blast_Score=107, Evalue=5e-23,
Organism=Drosophila melanogaster, GI281359715, Length=313, Percent_Identity=24.6006389776358, Blast_Score=106, Evalue=6e-23,
Organism=Drosophila melanogaster, GI281359713, Length=313, Percent_Identity=24.6006389776358, Blast_Score=106, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24639250, Length=313, Percent_Identity=24.6006389776358, Blast_Score=106, Evalue=6e-23,
Organism=Drosophila melanogaster, GI18543267, Length=313, Percent_Identity=24.6006389776358, Blast_Score=106, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24639252, Length=313, Percent_Identity=24.6006389776358, Blast_Score=106, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24585130, Length=282, Percent_Identity=24.468085106383, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24581020, Length=265, Percent_Identity=23.3962264150943, Blast_Score=68, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28573993, Length=265, Percent_Identity=23.3962264150943, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.7.1.4

Molecular weight: Translated: 83277; Mature: 83146

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKQRLVLIGNGMAGVRTAEEISLHGGSRFEIVIIGSEPHPGYNRILLSSVLQGETDWND
CCCCEEEEEECCCCCCCCHHHHEECCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCHHH
VMTKSRSWYEENGITLYTGETAVAIDTVNQTVATDQNREIAYDKLIIATGSSPFILPVHG
HHHHCCCHHHHCCEEEEECCEEEEEECCCHHHCCCCCCCEEEEEEEEEECCCCEEEEECC
ADKEGVYGFRTIDDCRAFINASKRFKKAAVIGGGILGLEAARGLANLGMKVDVVHHSSSI
CCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCHH
MQTQLDPPASAMLQKELERQGIHFLLEKDTEAILGTSRAEGVRFKDGTKISADLVVMAAG
HHHCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCEECCCCEEEEEEEEEEEC
VRPNIELAKASGISTNRAIIVSDYMETNVPNVYAVGECAEHNGTVYGLVKPLYEQGKVLA
CCCCCEEEECCCCCCCCEEEEECHHHCCCCCEEEECCHHCCCCEEEEHHHHHHHHHHHHH
KHICGLECSGYQGSVQSAALKMSGIDVFSAGKITEDDSTTAIKLLDEAAGVYKKAVFQGD
HHHCCCCCCCCCCCHHHHHEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
KMAGVILYGDTSGSQRLLESIIKQRDITVVKKELFQSEEDSSVASMAVRETICQCNAVSK
CEEEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCH
GMIMEAIQTHGLKTAEEVKRCTKASGSCGGCRPLVEELLLHTAEHDGHISAAEQPMCACT
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHC
SFTEDEVVNEIQMRNLSSVHEVISALGWKNSSGCRICVPALHYYLKMIRPDIAYEEHEEE
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCCCCHHCCCH
TDAIIPQMYGGRTNAEELKRIAGVIEKYQIQEVYMTHHQRLKLAGIKPEHIERVKEELGM
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCC
PYCLPQQQRIAAVKTCPCGSPQIQTLAADLEKAAESLLIPAKVSIGVSGCLDDCVYASVH
CCCCCCHHHHHHHEECCCCCCHHHHHHHHHHHHHHHEECCCEEECCHHHHHHHHHHHHHC
DIGLLKVNGGWEIYAGGQGGQHASPGELLSVADSAEEAGELMKGLLQYYRETANYLEKIG
CEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HWIERAGIIHIREVLFDNGLRGQLFERLEKEKRLAQQEMIKGLM
HHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKQRLVLIGNGMAGVRTAEEISLHGGSRFEIVIIGSEPHPGYNRILLSSVLQGETDWND
CCCEEEEEECCCCCCCCHHHHEECCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCHHH
VMTKSRSWYEENGITLYTGETAVAIDTVNQTVATDQNREIAYDKLIIATGSSPFILPVHG
HHHHCCCHHHHCCEEEEECCEEEEEECCCHHHCCCCCCCEEEEEEEEEECCCCEEEEECC
ADKEGVYGFRTIDDCRAFINASKRFKKAAVIGGGILGLEAARGLANLGMKVDVVHHSSSI
CCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCHH
MQTQLDPPASAMLQKELERQGIHFLLEKDTEAILGTSRAEGVRFKDGTKISADLVVMAAG
HHHCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCEECCCCEEEEEEEEEEEC
VRPNIELAKASGISTNRAIIVSDYMETNVPNVYAVGECAEHNGTVYGLVKPLYEQGKVLA
CCCCCEEEECCCCCCCCEEEEECHHHCCCCCEEEECCHHCCCCEEEEHHHHHHHHHHHHH
KHICGLECSGYQGSVQSAALKMSGIDVFSAGKITEDDSTTAIKLLDEAAGVYKKAVFQGD
HHHCCCCCCCCCCCHHHHHEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
KMAGVILYGDTSGSQRLLESIIKQRDITVVKKELFQSEEDSSVASMAVRETICQCNAVSK
CEEEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCH
GMIMEAIQTHGLKTAEEVKRCTKASGSCGGCRPLVEELLLHTAEHDGHISAAEQPMCACT
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHC
SFTEDEVVNEIQMRNLSSVHEVISALGWKNSSGCRICVPALHYYLKMIRPDIAYEEHEEE
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCCCCHHCCCH
TDAIIPQMYGGRTNAEELKRIAGVIEKYQIQEVYMTHHQRLKLAGIKPEHIERVKEELGM
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHCCC
PYCLPQQQRIAAVKTCPCGSPQIQTLAADLEKAAESLLIPAKVSIGVSGCLDDCVYASVH
CCCCCCHHHHHHHEECCCCCCHHHHHHHHHHHHHHHEECCCEEECCHHHHHHHHHHHHHC
DIGLLKVNGGWEIYAGGQGGQHASPGELLSVADSAEEAGELMKGLLQYYRETANYLEKIG
CEEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HWIERAGIIHIREVLFDNGLRGQLFERLEKEKRLAQQEMIKGLM
HHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD; NAD+; NADH; Siroheme [C]

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.4 {NO2-}} 0.005 {NO2-}} 0.016 {NADH}} 5.3 {hydroxylamine}} [C]

Substrates: ammonium hydroxide; NAD+; NADP+; H2O

Specific reaction: ammonium hydroxide + 3 NAD(P)+ + H2O = nitrite + 3 NAD(P)H + 3 H+

General reaction: Redox reaction [C]

Inhibitor: 1, 10-Phenanthroline; 2, 2'-Bipyridine; Arsenite; CN-; NAD+; NADP+; NaN3; p-Chloromercuribenzoate; SO32- [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]