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The map label for this gene is nasC [H]

Identifier: 52784303

GI number: 52784303

Start: 487496

End: 489655

Strand: Direct

Name: nasC [H]

Synonym: BLi00483

Alternate gene names: 52784303

Gene position: 487496-489655 (Clockwise)

Preceding gene: 52784302

Following gene: 52784304

Centisome position: 11.54

GC content: 49.58

Gene sequence:

>2160_bases
ATGACAGAACGATTGCTAAAGTACTTTCGCACAAAGCAAAAAGAAGTGCAATCCGAAAAAACGTATGACACCCAATGTCC
ATTTTGCAGCATGCAGTGCAAAATGCAGCTCATTGAACAGACGATTGTGACAAGAAAAAAATACACGGCGGTCGGAAAAG
ACAATCCGACGACAAAGGGGCGGCTGTGCATGAAAGGGATGAACGCCCATCAGCACGCGTTTCATTCAGAGAGAATCACC
CATCCGCTGATCAAAAAAAACGGCGAATTTGTCCGTGTATCATGGGAAGAAGCGCTTCAGTACATCAAAGAACAAGTAAC
AGCGATCCAGCATGAAGACGGCCGCGATGCTTTGAGTGTATACGGCAGCGCGTCCATTACAAACGAAGAAGCGTACCTGC
TCGGGAAGTTCGCCCGCGTCGCCTTGAAGACGAAGCATATTGACTACAACGGCAGGCTTTGCATGTCGGCGGCCGCAACA
GCTGCAAATCAAACCTTCGGAATGGACCGGGGACTGACAAATGCCCTTTCCGAGATCCCCCATGCAAGGGTCATCATCCT
CGCAGGTACGAATATCGCCGAGTGCCAGCCGACGATCATGCCGTATTTTGAAGAAGCGAAGCAAAACAGGGCGTACATCA
TCGTCATTGATCCCCGCGAAACGGCAACGGCCAAGATCGCCGACCTGCATTTGAAAATCAAGCCGGGAACAGACGCCGCG
CTCGCAAACGGAATGCTGAAAATCATCATTGAAGAACAGCTGACAGACGGAGCTTTTATCAGAGATAGAGCTGAAGGGTT
TGAAGAGGTCAAGCGCCATGTAGCATCTTTATCGCTTGAAGACATTTCGGCCAAAACCGGGATACAAATCGAGCAGATCA
AAAAAGCCGCGGTAAAATTCGCCCGCGAGGAGACGGGAATGCTGTTCACGGCAAGAGGGGTTGAACAGCAGACTGACGGA
ACGGCTGCTGTAAGAAATTTACTGAATATTCTAATATCAACGGGGAAAATCGGCAAATATGCTTGCGGCTACGGCGCCGT
CACCGGGCAAGGAAACGGGCAGGGGGCGCGGGAGCACGGGCAGAAAGCAGACCAGCTGCCAGGTTACCGTTCGATTGAAA
ATGAAGCCGACCGCGCCTATATTGCGGATGTTTGGGGCATAAAAGCTGAAGATCTTCCGGGAAAAGGCGTATCCGCTTAT
GAAATGATGAAGAAAGTTCATGAAGGCGAAATTACAGGAATGTTTTTAATGTGTTCAAACCCTGTTGTATCCAGCCCGAC
CGCCGGCTTTGTCAAATCCGCTTTGGGTAAATTAAAGTTTTTTGCCGCCGTTGATTTGTTTCTTTCTGAAACGGCAATAC
TCGCGGATGTCGTTCTCCCAGCATCATCCTATCTCGAGGACGAAGGAACGATGACCAATCTGGAAGGCAGAGTGACATTA
AGGGAAGCCTCAAAGCCGTGTCCCGGTGAAGCGAGGCATGACTGGCAAATTATTTGCGATATCGCTAAAGCGCTTGGAAA
AGGCCGCTATTTTTCATATCGATCGGCAGAGGATGTTTTTAATGAATTGAGAAAAGCGAGCCGCGGCGGAATTGCCGACT
ATTCGGGCATCACCTATGAACGTCTGAGAAAGGAAGGCGGCATTTTATGGCCCTGTCCAGAAGAGGGGCACCCTGGAACA
CAGCGCCTGTTTGAATCATCCTTCTCCCACCCGGACAAAAAAGCAAGAATGTGCGTCGTCCCGAACGAACCAAAGGTCAA
AAAAGATGTACCGACTGAGGAGTATCCCCTTATTCTGACGACCGGAAGGGTGATGTCTCACTATTTAACCGGGGTACAGA
CGAGAAGGAGCGCTGCGCTCAAGGCGAGACACTTTGAATCATTTATGGAAATCCATCCGAAAACGGCGGAAAAGTACAAT
ATTGGCGACCGGGTGCTGGTCAGGGTGGAATCAAAAAGAGGCAGCATCATCGTCAGGAGCAAATGGTCGGAAGCCATCAG
GCCGGATACCGTTTTTGTACCGATACATTGGGCGGATTCGCAAAATGTGAACGATTTGATAGCGGAAACGCTCGATCCGT
CCTGCAGCATGCCGGGATTTAAGGTTTGCTCCGTGAAAATTCGTCCGTCAGTTGACAATTTTGTGAATAAATTGTCATAA

Upstream 100 bases:

>100_bases
GAAGTTTTATTTGACAACGGACTTCGCGGCCAGCTGTTCGAACGGCTTGAAAAAGAAAAGCGGCTGGCTCAGCAAGAAAT
GATAAAAGGGCTGATGTGAA

Downstream 100 bases:

>100_bases
AAAGTCATAAAAAGTTTACAAAATTAACTATAATTTGTGATAGTTTTAAAGATGTAAATAGAATACTTGTTTAGGGCGTA
TAGTGAATTGTTTTGAAAAA

Product: NasC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 719; Mature: 718

Protein sequence:

>719_residues
MTERLLKYFRTKQKEVQSEKTYDTQCPFCSMQCKMQLIEQTIVTRKKYTAVGKDNPTTKGRLCMKGMNAHQHAFHSERIT
HPLIKKNGEFVRVSWEEALQYIKEQVTAIQHEDGRDALSVYGSASITNEEAYLLGKFARVALKTKHIDYNGRLCMSAAAT
AANQTFGMDRGLTNALSEIPHARVIILAGTNIAECQPTIMPYFEEAKQNRAYIIVIDPRETATAKIADLHLKIKPGTDAA
LANGMLKIIIEEQLTDGAFIRDRAEGFEEVKRHVASLSLEDISAKTGIQIEQIKKAAVKFAREETGMLFTARGVEQQTDG
TAAVRNLLNILISTGKIGKYACGYGAVTGQGNGQGAREHGQKADQLPGYRSIENEADRAYIADVWGIKAEDLPGKGVSAY
EMMKKVHEGEITGMFLMCSNPVVSSPTAGFVKSALGKLKFFAAVDLFLSETAILADVVLPASSYLEDEGTMTNLEGRVTL
REASKPCPGEARHDWQIICDIAKALGKGRYFSYRSAEDVFNELRKASRGGIADYSGITYERLRKEGGILWPCPEEGHPGT
QRLFESSFSHPDKKARMCVVPNEPKVKKDVPTEEYPLILTTGRVMSHYLTGVQTRRSAALKARHFESFMEIHPKTAEKYN
IGDRVLVRVESKRGSIIVRSKWSEAIRPDTVFVPIHWADSQNVNDLIAETLDPSCSMPGFKVCSVKIRPSVDNFVNKLS

Sequences:

>Translated_719_residues
MTERLLKYFRTKQKEVQSEKTYDTQCPFCSMQCKMQLIEQTIVTRKKYTAVGKDNPTTKGRLCMKGMNAHQHAFHSERIT
HPLIKKNGEFVRVSWEEALQYIKEQVTAIQHEDGRDALSVYGSASITNEEAYLLGKFARVALKTKHIDYNGRLCMSAAAT
AANQTFGMDRGLTNALSEIPHARVIILAGTNIAECQPTIMPYFEEAKQNRAYIIVIDPRETATAKIADLHLKIKPGTDAA
LANGMLKIIIEEQLTDGAFIRDRAEGFEEVKRHVASLSLEDISAKTGIQIEQIKKAAVKFAREETGMLFTARGVEQQTDG
TAAVRNLLNILISTGKIGKYACGYGAVTGQGNGQGAREHGQKADQLPGYRSIENEADRAYIADVWGIKAEDLPGKGVSAY
EMMKKVHEGEITGMFLMCSNPVVSSPTAGFVKSALGKLKFFAAVDLFLSETAILADVVLPASSYLEDEGTMTNLEGRVTL
REASKPCPGEARHDWQIICDIAKALGKGRYFSYRSAEDVFNELRKASRGGIADYSGITYERLRKEGGILWPCPEEGHPGT
QRLFESSFSHPDKKARMCVVPNEPKVKKDVPTEEYPLILTTGRVMSHYLTGVQTRRSAALKARHFESFMEIHPKTAEKYN
IGDRVLVRVESKRGSIIVRSKWSEAIRPDTVFVPIHWADSQNVNDLIAETLDPSCSMPGFKVCSVKIRPSVDNFVNKLS
>Mature_718_residues
TERLLKYFRTKQKEVQSEKTYDTQCPFCSMQCKMQLIEQTIVTRKKYTAVGKDNPTTKGRLCMKGMNAHQHAFHSERITH
PLIKKNGEFVRVSWEEALQYIKEQVTAIQHEDGRDALSVYGSASITNEEAYLLGKFARVALKTKHIDYNGRLCMSAAATA
ANQTFGMDRGLTNALSEIPHARVIILAGTNIAECQPTIMPYFEEAKQNRAYIIVIDPRETATAKIADLHLKIKPGTDAAL
ANGMLKIIIEEQLTDGAFIRDRAEGFEEVKRHVASLSLEDISAKTGIQIEQIKKAAVKFAREETGMLFTARGVEQQTDGT
AAVRNLLNILISTGKIGKYACGYGAVTGQGNGQGAREHGQKADQLPGYRSIENEADRAYIADVWGIKAEDLPGKGVSAYE
MMKKVHEGEITGMFLMCSNPVVSSPTAGFVKSALGKLKFFAAVDLFLSETAILADVVLPASSYLEDEGTMTNLEGRVTLR
EASKPCPGEARHDWQIICDIAKALGKGRYFSYRSAEDVFNELRKASRGGIADYSGITYERLRKEGGILWPCPEEGHPGTQ
RLFESSFSHPDKKARMCVVPNEPKVKKDVPTEEYPLILTTGRVMSHYLTGVQTRRSAALKARHFESFMEIHPKTAEKYNI
GDRVLVRVESKRGSIIVRSKWSEAIRPDTVFVPIHWADSQNVNDLIAETLDPSCSMPGFKVCSVKIRPSVDNFVNKLS

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=701, Percent_Identity=32.5249643366619, Blast_Score=370, Evalue=1e-103,
Organism=Escherichia coli, GI1788534, Length=788, Percent_Identity=25.253807106599, Blast_Score=224, Evalue=2e-59,
Organism=Escherichia coli, GI3868719, Length=614, Percent_Identity=24.5928338762215, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI3868720, Length=630, Percent_Identity=23.6507936507936, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI87081797, Length=694, Percent_Identity=22.7665706051873, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI171474008, Length=617, Percent_Identity=22.2042139384117, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787870, Length=358, Percent_Identity=27.0949720670391, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1787778, Length=516, Percent_Identity=21.8992248062016, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081994, Length=502, Percent_Identity=24.1035856573705, Blast_Score=73, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 79849; Mature: 79718

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1 ; PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERLLKYFRTKQKEVQSEKTYDTQCPFCSMQCKMQLIEQTIVTRKKYTAVGKDNPTTKG
CHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC
RLCMKGMNAHQHAFHSERITHPLIKKNGEFVRVSWEEALQYIKEQVTAIQHEDGRDALSV
HHHCCCCCHHHHHHHHHHCCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCHHHH
YGSASITNEEAYLLGKFARVALKTKHIDYNGRLCMSAAATAANQTFGMDRGLTNALSEIP
CCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCEEHHHHHHHHHHHCCCCCCHHHHHHHCC
HARVIILAGTNIAECQPTIMPYFEEAKQNRAYIIVIDPRETATAKIADLHLKIKPGTDAA
CEEEEEEECCCHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEEEECCCCCHH
LANGMLKIIIEEQLTDGAFIRDRAEGFEEVKRHVASLSLEDISAKTGIQIEQIKKAAVKF
HHCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHH
AREETGMLFTARGVEQQTDGTAAVRNLLNILISTGKIGKYACGYGAVTGQGNGQGAREHG
HHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCEECCCCCCCCHHHC
QKADQLPGYRSIENEADRAYIADVWGIKAEDLPGKGVSAYEMMKKVHEGEITGMFLMCSN
CHHHHCCCCCCCCCCCCCEEEEHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECC
PVVSSPTAGFVKSALGKLKFFAAVDLFLSETAILADVVLPASSYLEDEGTMTNLEGRVTL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEECCCCEEEE
REASKPCPGEARHDWQIICDIAKALGKGRYFSYRSAEDVFNELRKASRGGIADYSGITYE
ECCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCCCCCCCHHH
RLRKEGGILWPCPEEGHPGTQRLFESSFSHPDKKARMCVVPNEPKVKKDVPTEEYPLILT
HHHHCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEE
TGRVMSHYLTGVQTRRSAALKARHFESFMEIHPKTAEKYNIGDRVLVRVESKRGSIIVRS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEE
KWSEAIRPDTVFVPIHWADSQNVNDLIAETLDPSCSMPGFKVCSVKIRPSVDNFVNKLS
CCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHCC
>Mature Secondary Structure 
TERLLKYFRTKQKEVQSEKTYDTQCPFCSMQCKMQLIEQTIVTRKKYTAVGKDNPTTKG
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCC
RLCMKGMNAHQHAFHSERITHPLIKKNGEFVRVSWEEALQYIKEQVTAIQHEDGRDALSV
HHHCCCCCHHHHHHHHHHCCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCHHHH
YGSASITNEEAYLLGKFARVALKTKHIDYNGRLCMSAAATAANQTFGMDRGLTNALSEIP
CCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCEEHHHHHHHHHHHCCCCCCHHHHHHHCC
HARVIILAGTNIAECQPTIMPYFEEAKQNRAYIIVIDPRETATAKIADLHLKIKPGTDAA
CEEEEEEECCCHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEEEECCCCCHH
LANGMLKIIIEEQLTDGAFIRDRAEGFEEVKRHVASLSLEDISAKTGIQIEQIKKAAVKF
HHCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHH
AREETGMLFTARGVEQQTDGTAAVRNLLNILISTGKIGKYACGYGAVTGQGNGQGAREHG
HHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCEECCCCCCCCHHHC
QKADQLPGYRSIENEADRAYIADVWGIKAEDLPGKGVSAYEMMKKVHEGEITGMFLMCSN
CHHHHCCCCCCCCCCCCCEEEEHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECC
PVVSSPTAGFVKSALGKLKFFAAVDLFLSETAILADVVLPASSYLEDEGTMTNLEGRVTL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCEECCCCEEEE
REASKPCPGEARHDWQIICDIAKALGKGRYFSYRSAEDVFNELRKASRGGIADYSGITYE
ECCCCCCCCCCCCCHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCCCCCCCHHH
RLRKEGGILWPCPEEGHPGTQRLFESSFSHPDKKARMCVVPNEPKVKKDVPTEEYPLILT
HHHHCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEEE
TGRVMSHYLTGVQTRRSAALKARHFESFMEIHPKTAEKYNIGDRVLVRVESKRGSIIVRS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEE
KWSEAIRPDTVFVPIHWADSQNVNDLIAETLDPSCSMPGFKVCSVKIRPSVDNFVNKLS
CCCCCCCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 7868621 [H]