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The map label for this gene is nadE

Identifier: 52784192

GI number: 52784192

Start: 347150

End: 347968

Strand: Direct

Name: nadE

Synonym: BLi00370

Alternate gene names: 52784192

Gene position: 347150-347968 (Clockwise)

Preceding gene: 52784190

Following gene: 52784193

Centisome position: 8.22

GC content: 49.33

Gene sequence:

>819_bases
ATGACTTTACAAGAAAAAATCATGCAGGAACTGAATGTAAAGCCTTCTATCGAACCGAAACAGGAAATTGAAAAAAGGGT
CGGTTTTTTAAAAAGCTATTTGAAAAAAACGGGTGCCAAAGGCTTTGTTTTAGGCATCAGCGGAGGACAGGACTCCACAT
TGGCGGGCAGGCTGGCCCAGCTGGCTGTTGAAGAATTGAGGGAAGAAGGCATTCAGGCGGAATTTATTGCGGTCCGCTTG
CCTTACGGTGTTCAGCAGGATGAAGATGACGCCCAGCTGGCGCTGAAATTTATCCAGCCGGACAAGTCTTTTGCTTTTGA
CATTGCTTCAACTGTAGGTTCATTTGCTGCTCAATATCAGTCAGTCACGGGAGAGGCGCTTGCCGATTTTCACAAAGGCA
ATGTAAAAGCGAGAGTCCGGATGATCACCCAATATGCGATCGGAGGTCAAAATCAGCTGCTGGTAATTGGGACAGATCAT
GCTGCAGAAGCTGTTACCGGATTTTTTACGAAATACGGAGACGGGGGCGCAGACCTTTTGCCGCTTACAGGACTGACAAA
GCGCCAGGGCAGAAGCCTGCTGGAAGAGCTGGGAGCGCCGGAGCGGCTTTATACGAAGTCCCCGACCGCCGATTTGCTTG
ATGAAAAACCTCAGCAATCCGATGAAACAGAGCTCGGCCTGACATATGATAACATCGACGACTACCTTGAAGGAAAAGCC
GTCTCCAGCGAGGTGGCAGAGGCCATCGAAAAACGCTACAAGGCATCCGAGCACAAACGCCAAGTCCCTGCTTCAATGTT
TGACGACTGGTGGAAATAA

Upstream 100 bases:

>100_bases
AGGTTCTTTGCGCACCTCAATATTCACTTTGAAATGAGACATATGCTATAGTTAAAGACATAATCATTACGGTTCATTCA
GTCAAAAGGAGGAGTGACGG

Downstream 100 bases:

>100_bases
GCTAAAAAGAGGCTGAAGTCAGCCTCTTTTTTTATTAACAGGCACCCTATATCATTACAGATGGAATTGTCATATACTTA
AGATATTTGATTCCTTTATG

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MTLQEKIMQELNVKPSIEPKQEIEKRVGFLKSYLKKTGAKGFVLGISGGQDSTLAGRLAQLAVEELREEGIQAEFIAVRL
PYGVQQDEDDAQLALKFIQPDKSFAFDIASTVGSFAAQYQSVTGEALADFHKGNVKARVRMITQYAIGGQNQLLVIGTDH
AAEAVTGFFTKYGDGGADLLPLTGLTKRQGRSLLEELGAPERLYTKSPTADLLDEKPQQSDETELGLTYDNIDDYLEGKA
VSSEVAEAIEKRYKASEHKRQVPASMFDDWWK

Sequences:

>Translated_272_residues
MTLQEKIMQELNVKPSIEPKQEIEKRVGFLKSYLKKTGAKGFVLGISGGQDSTLAGRLAQLAVEELREEGIQAEFIAVRL
PYGVQQDEDDAQLALKFIQPDKSFAFDIASTVGSFAAQYQSVTGEALADFHKGNVKARVRMITQYAIGGQNQLLVIGTDH
AAEAVTGFFTKYGDGGADLLPLTGLTKRQGRSLLEELGAPERLYTKSPTADLLDEKPQQSDETELGLTYDNIDDYLEGKA
VSSEVAEAIEKRYKASEHKRQVPASMFDDWWK
>Mature_271_residues
TLQEKIMQELNVKPSIEPKQEIEKRVGFLKSYLKKTGAKGFVLGISGGQDSTLAGRLAQLAVEELREEGIQAEFIAVRLP
YGVQQDEDDAQLALKFIQPDKSFAFDIASTVGSFAAQYQSVTGEALADFHKGNVKARVRMITQYAIGGQNQLLVIGTDHA
AEAVTGFFTKYGDGGADLLPLTGLTKRQGRSLLEELGAPERLYTKSPTADLLDEKPQQSDETELGLTYDNIDDYLEGKAV
SSEVAEAIEKRYKASEHKRQVPASMFDDWWK

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=277, Percent_Identity=56.3176895306859, Blast_Score=299, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI17538756, Length=264, Percent_Identity=26.1363636363636, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_BACLD (Q65NN6)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_077612.1
- RefSeq:   YP_090021.1
- HSSP:   P08164
- ProteinModelPortal:   Q65NN6
- SMR:   Q65NN6
- STRING:   Q65NN6
- EnsemblBacteria:   EBBACT00000055073
- EnsemblBacteria:   EBBACT00000062619
- GeneID:   3028255
- GeneID:   3099555
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00370
- KEGG:   bli:BL01706
- NMPDR:   fig|279010.5.peg.2223
- eggNOG:   COG0171
- GeneTree:   EBGT00050000001308
- HOGENOM:   HBG351567
- OMA:   KVAQATI
- ProtClustDB:   PRK00768
- BioCyc:   BLIC279010-1:BLI00370-MONOMER
- BioCyc:   BLIC279010:BL01706-MONOMER
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 29997; Mature: 29866

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: ACT_SITE 47-47

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQEKIMQELNVKPSIEPKQEIEKRVGFLKSYLKKTGAKGFVLGISGGQDSTLAGRLAQ
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
LAVEELREEGIQAEFIAVRLPYGVQQDEDDAQLALKFIQPDKSFAFDIASTVGSFAAQYQ
HHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SVTGEALADFHKGNVKARVRMITQYAIGGQNQLLVIGTDHAAEAVTGFFTKYGDGGADLL
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCEE
PLTGLTKRQGRSLLEELGAPERLYTKSPTADLLDEKPQQSDETELGLTYDNIDDYLEGKA
CCCCCCHHHHHHHHHHCCCCHHHHCCCCCHHHHHCCCCCCCCCCCCCCHHCHHHHHCCCH
VSSEVAEAIEKRYKASEHKRQVPASMFDDWWK
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure 
TLQEKIMQELNVKPSIEPKQEIEKRVGFLKSYLKKTGAKGFVLGISGGQDSTLAGRLAQ
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
LAVEELREEGIQAEFIAVRLPYGVQQDEDDAQLALKFIQPDKSFAFDIASTVGSFAAQYQ
HHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SVTGEALADFHKGNVKARVRMITQYAIGGQNQLLVIGTDHAAEAVTGFFTKYGDGGADLL
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCEE
PLTGLTKRQGRSLLEELGAPERLYTKSPTADLLDEKPQQSDETELGLTYDNIDDYLEGKA
CCCCCCHHHHHHHHHHCCCCHHHHCCCCCHHHHHCCCCCCCCCCCCCCHHCHHHHHCCCH
VSSEVAEAIEKRYKASEHKRQVPASMFDDWWK
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA