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The map label for this gene is ldh

Identifier: 52784188

GI number: 52784188

Start: 342270

End: 343229

Strand: Direct

Name: ldh

Synonym: BLi00366

Alternate gene names: 52784188

Gene position: 342270-343229 (Clockwise)

Preceding gene: 52784186

Following gene: 52784189

Centisome position: 8.11

GC content: 45.83

Gene sequence:

>960_bases
ATGAGAAACCAAAAAGTCAATCGTACAGTTTTGATCGGTGCGGGTTTTGTTGGAAGCAGCTATGCATTTACGTTAATTAA
CCAAGGTATCACAGACGAACTTGTGATCATTGATTTAAATAAAGACAAAGCAATGGGCGATGTTATGGATCTCAACCACG
GTAAAGCATTTGCTCCGCATCCTGTGAAAACATGGTACGGCACATATGATGACTGCAAAGAAGCTGATATTGTCTGCGTA
TGCGCCGGAGCGAACCAAAAGCCGGGTGAAACCCGCCTTGATCTAGTCGAAAAGAACTTGAAAATTTTTAAAGGAATCAT
CGGAGAAGTCATGGCCAGCGGTTTTGACGGCATCTTCCTCGTCGCTACAAACCCTGTCGACATCTTGACTTATGCGACTT
GGAAATTCAGCGGCCTTCCAAAAGAACGCGTCATCGGAAGCGGCACAACGCTTGACACTGCCCGTTTCCGCTACTTGCTC
AGCGAGTATTTCGGTGTCGCTGCCCACAATGCGCACGGCTATATCATTGGTGAACACGGAGATACGGAGCTGCCTGTCTG
GAGCCATGCGAATATCGGCGGAGTTCCCGTCAGCGACTTGCTGAAAAGAAATGAGAAATACAAAGCGGAAGATCTAGATG
AACTGTTTGACAATGTAAAAAACGCCGCTTACCACATTATTGAGAAAAAAGGCGCGACATACTACGGAGTTGCCATGAGC
CTCGCACGTATTACGAAAGCGATTTATCGAAATGAAGAAGCAATTTTGACCGTGAGCGCTCATCTTGATGGTGAATTTGG
AGAAAATGATGTTTATATCGGAGTTCCGGCGGTTGTCGGCCGATGCGGAGCGAGGGAAATTGTTGAGCTCGATCTGAACG
AAAAGGAAAAACAACAATTCAAGCATAGCGCAGGTGTGTTAAAAGCAATTTTAAAACCGCATTTTGAACTGCAGGCGTAA

Upstream 100 bases:

>100_bases
TAAATAAAATTGAAAAAGTATGTGAAGTATTGCACAATATGAATGTGAAGAATTTCACAAACAGCTTAGTTTAAAACAAA
CGGCAAAGGAATGATGAGTC

Downstream 100 bases:

>100_bases
CTGAACACCATGAAGATACTAACATCATGAAGATTGCGAGATGATGAAAATGTGGCAGCAGATATACGATCCGTTTGGAA
ATCTGTTTATCAGCGCTTTT

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MRNQKVNRTVLIGAGFVGSSYAFTLINQGITDELVIIDLNKDKAMGDVMDLNHGKAFAPHPVKTWYGTYDDCKEADIVCV
CAGANQKPGETRLDLVEKNLKIFKGIIGEVMASGFDGIFLVATNPVDILTYATWKFSGLPKERVIGSGTTLDTARFRYLL
SEYFGVAAHNAHGYIIGEHGDTELPVWSHANIGGVPVSDLLKRNEKYKAEDLDELFDNVKNAAYHIIEKKGATYYGVAMS
LARITKAIYRNEEAILTVSAHLDGEFGENDVYIGVPAVVGRCGAREIVELDLNEKEKQQFKHSAGVLKAILKPHFELQA

Sequences:

>Translated_319_residues
MRNQKVNRTVLIGAGFVGSSYAFTLINQGITDELVIIDLNKDKAMGDVMDLNHGKAFAPHPVKTWYGTYDDCKEADIVCV
CAGANQKPGETRLDLVEKNLKIFKGIIGEVMASGFDGIFLVATNPVDILTYATWKFSGLPKERVIGSGTTLDTARFRYLL
SEYFGVAAHNAHGYIIGEHGDTELPVWSHANIGGVPVSDLLKRNEKYKAEDLDELFDNVKNAAYHIIEKKGATYYGVAMS
LARITKAIYRNEEAILTVSAHLDGEFGENDVYIGVPAVVGRCGAREIVELDLNEKEKQQFKHSAGVLKAILKPHFELQA
>Mature_319_residues
MRNQKVNRTVLIGAGFVGSSYAFTLINQGITDELVIIDLNKDKAMGDVMDLNHGKAFAPHPVKTWYGTYDDCKEADIVCV
CAGANQKPGETRLDLVEKNLKIFKGIIGEVMASGFDGIFLVATNPVDILTYATWKFSGLPKERVIGSGTTLDTARFRYLL
SEYFGVAAHNAHGYIIGEHGDTELPVWSHANIGGVPVSDLLKRNEKYKAEDLDELFDNVKNAAYHIIEKKGATYYGVAMS
LARITKAIYRNEEAILTVSAHLDGEFGENDVYIGVPAVVGRCGAREIVELDLNEKEKQQFKHSAGVLKAILKPHFELQA

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family

Homologues:

Organism=Homo sapiens, GI47059044, Length=311, Percent_Identity=38.9067524115756, Blast_Score=238, Evalue=8e-63,
Organism=Homo sapiens, GI221136809, Length=311, Percent_Identity=38.9067524115756, Blast_Score=238, Evalue=8e-63,
Organism=Homo sapiens, GI15082234, Length=309, Percent_Identity=38.1877022653722, Blast_Score=236, Evalue=1e-62,
Organism=Homo sapiens, GI291575128, Length=308, Percent_Identity=38.6363636363636, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI4557032, Length=308, Percent_Identity=38.6363636363636, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI5031857, Length=319, Percent_Identity=38.5579937304075, Blast_Score=234, Evalue=8e-62,
Organism=Homo sapiens, GI260099723, Length=319, Percent_Identity=38.5579937304075, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI9257228, Length=307, Percent_Identity=39.7394136807818, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI4504973, Length=307, Percent_Identity=39.7394136807818, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI260099727, Length=194, Percent_Identity=44.3298969072165, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI260099725, Length=194, Percent_Identity=44.3298969072165, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI207028494, Length=237, Percent_Identity=37.9746835443038, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI103472011, Length=311, Percent_Identity=25.4019292604502, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI103472015, Length=187, Percent_Identity=29.9465240641711, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535107, Length=293, Percent_Identity=41.6382252559727, Blast_Score=239, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17554310, Length=261, Percent_Identity=25.6704980842912, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI17136226, Length=308, Percent_Identity=39.9350649350649, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI45550422, Length=311, Percent_Identity=28.6173633440514, Blast_Score=163, Evalue=1e-40,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): LDH_BACLD (Q65NP0)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_077608.1
- RefSeq:   YP_090017.1
- ProteinModelPortal:   Q65NP0
- SMR:   Q65NP0
- STRING:   Q65NP0
- EnsemblBacteria:   EBBACT00000058148
- EnsemblBacteria:   EBBACT00000060867
- GeneID:   3029440
- GeneID:   3101432
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00366
- KEGG:   bli:BL01703
- NMPDR:   fig|279010.5.peg.2219
- eggNOG:   COG0039
- GeneTree:   EBGT00050000001153
- HOGENOM:   HBG566126
- OMA:   MDLNHGV
- ProtClustDB:   PRK00066
- BioCyc:   BLIC279010-1:BLI00366-MONOMER
- BioCyc:   BLIC279010:BL01703-MONOMER
- GO:   GO:0005737
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00488
- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01771

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.27

Molecular weight: Translated: 35111; Mature: 35111

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00064 L_LDH

Important sites: ACT_SITE 179-179 BINDING 92-92 BINDING 124-124 BINDING 155-155 BINDING 233-233

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNQKVNRTVLIGAGFVGSSYAFTLINQGITDELVIIDLNKDKAMGDVMDLNHGKAFAPH
CCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCHHHCCCCCCCCCCC
PVKTWYGTYDDCKEADIVCVCAGANQKPGETRLDLVEKNLKIFKGIIGEVMASGFDGIFL
CCHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VATNPVDILTYATWKFSGLPKERVIGSGTTLDTARFRYLLSEYFGVAAHNAHGYIIGEHG
EECCCEEEEEEEEEEECCCCHHHEECCCCCCHHHHHHHHHHHHHCCEEECCCEEEEECCC
DTELPVWSHANIGGVPVSDLLKRNEKYKAEDLDELFDNVKNAAYHIIEKKGATYYGVAMS
CCCCCEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHH
LARITKAIYRNEEAILTVSAHLDGEFGENDVYIGVPAVVGRCGAREIVELDLNEKEKQQF
HHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEECHHHHCCCCCCEEEEECCCHHHHHHH
KHSAGVLKAILKPHFELQA
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRNQKVNRTVLIGAGFVGSSYAFTLINQGITDELVIIDLNKDKAMGDVMDLNHGKAFAPH
CCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCHHHCCCCCCCCCCC
PVKTWYGTYDDCKEADIVCVCAGANQKPGETRLDLVEKNLKIFKGIIGEVMASGFDGIFL
CCHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VATNPVDILTYATWKFSGLPKERVIGSGTTLDTARFRYLLSEYFGVAAHNAHGYIIGEHG
EECCCEEEEEEEEEEECCCCHHHEECCCCCCHHHHHHHHHHHHHCCEEECCCEEEEECCC
DTELPVWSHANIGGVPVSDLLKRNEKYKAEDLDELFDNVKNAAYHIIEKKGATYYGVAMS
CCCCCEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHH
LARITKAIYRNEEAILTVSAHLDGEFGENDVYIGVPAVVGRCGAREIVELDLNEKEKQQF
HHHHHHHHHCCCCEEEEEEEEECCCCCCCCEEEECHHHHCCCCCCEEEEECCCHHHHHHH
KHSAGVLKAILKPHFELQA
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA