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The map label for this gene is map [H]

Identifier: 52783993

GI number: 52783993

Start: 144440

End: 145186

Strand: Direct

Name: map [H]

Synonym: BLi00155

Alternate gene names: 52783993

Gene position: 144440-145186 (Clockwise)

Preceding gene: 52783992

Following gene: 52783994

Centisome position: 3.42

GC content: 47.52

Gene sequence:

>747_bases
ATGATTATTTGTAAAACTCCCCGAGAAATCGAAATCATGCGGGAGGCCGGACGAATTGTCGCTTTAACTCATCAGGAGCT
AAAAAAGCACATTAAACCCGGCATCTCGACAAAAGAATTGGATCAAATTGCCGAACGTTTTATTACAAAGCAAGGTGCAA
TCCCATCTTTTAAAGGGTATAATGGTTTTCGCGGGAGCATTTGCGTTTCGGTCAACGAAGAACTCGTGCACGGTATTCCG
GGAAAACGAGTCCTTCGCGACGGGGACATCATCAGCATCGATATCGGTGCGAAGCTAAACGGTTATCATGGTGACTCCGC
TTGGACTTATCCAGTGGGTACGATCAGCGATGATGATCAGAAGCTGCTGGATGTGACAGAGGAGTCTTTATATAGAGGCT
TGAAGGAAGCAAAACCGGGAGAACGTTTGTCGAATATTTCCCACGCAATACAAACGTATGTCGAAAGCGAACAATTTTCA
GTCGTCAGGGAATATGTAGGACACGGGGTAGGGCAGGATCTGCATGAGGATCCGCAAATTCCTCACTACGGTCCGCCGAA
CAAAGGCCCGCGTTTAAAGCCTGGCATGGTCTTGGCCATTGAGCCGATGGTCAATGCAGGTACACGCTATGTCAAAACAT
TGGCTGACAACTGGACGGTTGTAACTGTGGACGGGAAGAAGTGTGCTCACTTTGAACACACGATTGCCATTACGGATGCC
GGTTTTGAGATACTGACAAAAGCCTAG

Upstream 100 bases:

>100_bases
ATTTCTATGAAGATAAAGGATATCTCGTAAACATTGACGGGCAGAAGCACATCAATGAAGTTTACGCTGATATAAAGGAG
CTTCTCGGAGGCCGGGACAA

Downstream 100 bases:

>100_bases
GTGAAATGAGATGTATCGGAAAACACCTGAAATTGGACAGTTCGTACGCATGACAAGAGGCCGCGAAATGAACCAATACG
CAGTTGTTATTGATATAGTC

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP 1; Peptidase M [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MIICKTPREIEIMREAGRIVALTHQELKKHIKPGISTKELDQIAERFITKQGAIPSFKGYNGFRGSICVSVNEELVHGIP
GKRVLRDGDIISIDIGAKLNGYHGDSAWTYPVGTISDDDQKLLDVTEESLYRGLKEAKPGERLSNISHAIQTYVESEQFS
VVREYVGHGVGQDLHEDPQIPHYGPPNKGPRLKPGMVLAIEPMVNAGTRYVKTLADNWTVVTVDGKKCAHFEHTIAITDA
GFEILTKA

Sequences:

>Translated_248_residues
MIICKTPREIEIMREAGRIVALTHQELKKHIKPGISTKELDQIAERFITKQGAIPSFKGYNGFRGSICVSVNEELVHGIP
GKRVLRDGDIISIDIGAKLNGYHGDSAWTYPVGTISDDDQKLLDVTEESLYRGLKEAKPGERLSNISHAIQTYVESEQFS
VVREYVGHGVGQDLHEDPQIPHYGPPNKGPRLKPGMVLAIEPMVNAGTRYVKTLADNWTVVTVDGKKCAHFEHTIAITDA
GFEILTKA
>Mature_248_residues
MIICKTPREIEIMREAGRIVALTHQELKKHIKPGISTKELDQIAERFITKQGAIPSFKGYNGFRGSICVSVNEELVHGIP
GKRVLRDGDIISIDIGAKLNGYHGDSAWTYPVGTISDDDQKLLDVTEESLYRGLKEAKPGERLSNISHAIQTYVESEQFS
VVREYVGHGVGQDLHEDPQIPHYGPPNKGPRLKPGMVLAIEPMVNAGTRYVKTLADNWTVVTVDGKKCAHFEHTIAITDA
GFEILTKA

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=239, Percent_Identity=40.5857740585774, Blast_Score=187, Evalue=5e-48,
Organism=Homo sapiens, GI40385867, Length=243, Percent_Identity=37.037037037037, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI260593665, Length=290, Percent_Identity=23.1034482758621, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI149589008, Length=286, Percent_Identity=23.0769230769231, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1786364, Length=244, Percent_Identity=47.1311475409836, Blast_Score=232, Evalue=1e-62,
Organism=Escherichia coli, GI1789275, Length=222, Percent_Identity=26.1261261261261, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1788728, Length=207, Percent_Identity=27.0531400966184, Blast_Score=74, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI71996291, Length=243, Percent_Identity=38.2716049382716, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6323273, Length=218, Percent_Identity=44.0366972477064, Blast_Score=188, Evalue=7e-49,
Organism=Drosophila melanogaster, GI21355531, Length=239, Percent_Identity=41.0041841004184, Blast_Score=192, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24583427, Length=243, Percent_Identity=37.8600823045267, Blast_Score=176, Evalue=9e-45,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 27425; Mature: 27425

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00680 MAP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIICKTPREIEIMREAGRIVALTHQELKKHIKPGISTKELDQIAERFITKQGAIPSFKGY
CEEECCCHHHHHHHHCCCEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
NGFRGSICVSVNEELVHGIPGKRVLRDGDIISIDIGAKLNGYHGDSAWTYPVGTISDDDQ
CCCCCEEEEEECHHHHHCCCCCCEECCCCEEEEEECCEECCEECCCCEEEECCCCCCCHH
KLLDVTEESLYRGLKEAKPGERLSNISHAIQTYVESEQFSVVREYVGHGVGQDLHEDPQI
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PHYGPPNKGPRLKPGMVLAIEPMVNAGTRYVKTLADNWTVVTVDGKKCAHFEHTIAITDA
CCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCEEEEEECCCCCCCEEEEEEEECC
GFEILTKA
CEEEEECC
>Mature Secondary Structure
MIICKTPREIEIMREAGRIVALTHQELKKHIKPGISTKELDQIAERFITKQGAIPSFKGY
CEEECCCHHHHHHHHCCCEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
NGFRGSICVSVNEELVHGIPGKRVLRDGDIISIDIGAKLNGYHGDSAWTYPVGTISDDDQ
CCCCCEEEEEECHHHHHCCCCCCEECCCCEEEEEECCEECCEECCCCEEEECCCCCCCHH
KLLDVTEESLYRGLKEAKPGERLSNISHAIQTYVESEQFSVVREYVGHGVGQDLHEDPQI
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PHYGPPNKGPRLKPGMVLAIEPMVNAGTRYVKTLADNWTVVTVDGKKCAHFEHTIAITDA
CCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCEEEEEECCCCCCCEEEEEEEECC
GFEILTKA
CEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2113521; 8635744; 9384377 [H]