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The map label for this gene is pbpF [H]

Identifier: 52144243

GI number: 52144243

Start: 1089590

End: 1091707

Strand: Direct

Name: pbpF [H]

Synonym: BCZK0985

Alternate gene names: 52144243

Gene position: 1089590-1091707 (Clockwise)

Preceding gene: 52144244

Following gene: 52144241

Centisome position: 20.55

GC content: 39.24

Gene sequence:

>2118_bases
ATGAAGAAAGTAAAGTGGACTTTATTAGGTGGGTTAGCAACGCTTGTATTAGCGATTGTACTTTATAAACTTATCGTGTT
AGCTGGTGGCTATATGATGGATGAAAAGCAGCTCGTTTTCCACTCTTCATCACGTATCGTTGACCAGAAAGGGAAAGAAA
TTACGAAATTATACGTAGAAAATAGAGATATCGTACCAATCGAGCAAATTCCAAAATACGTGCAACAGTCGTTTATTGCG
GTGGAAGATTCTCGTTTTTATGAGCATCAAGGAATTGATTACCCGTCTATATTCCGTGCTCTTTATAAAGACACGTTAGC
TGGAGAGAAGGTCGAAGGCGGTAGTACGATTACGCAGCAACTTGCTAAAAACGTCTTTTTAACTCGTGAAAAAACATTTA
CGCGCAAGTTGAAGGAAGTCGCAATTTCCCTTCAATTAGAGCAAAAATATACGAAGCAACAAATTCTTGAAATGTATATG
AATCATATTTATTTTGGCCATGGGGCTTACGGTATTCAAGCGGCAGCAAAGTTATATTTTAATAAAAATGTAGAGGATTT
AACAGTAGAAGAAGGGGCAATGCTTGCAGGTCTTCCGAAGTCGCCAAACGGATATTCACCTTATTTTTCTCCAGAGAAGA
GTAAAGAACGCCGTGATCTCGTATTGTCACTTATGCATAAACAAGGCTATTTGACTGCCGAAGAAAGCGTACGTTATCAA
GGGAAGACAATTGCTCTATATAAAAACTTAGATGAGAATGAACTCGCATATATGCCGTATATTGATATGGTTATAGATGA
AGCAGCTCGTTTATACGGATTGTCTCATCAAGAAGTGCTTCGCGGAGGATATACGTTTGTCGTACCGATGGATGAAAAAA
TTCAAAAGGTAGCGTATAACCAGTTTCAAGATGCAAGGAATTTCCCTGGGAAAGAAGAGGGGGCGCAAGGTGCTTTTTTA
TTAATGGATAATCGTACGGGCGGAATTAAAGCTGCGATTGGCGGAAGAAAGTACGTCCCGAGAGGATTCAATCGTGTTTT
TGCAAAAAGACAGCCCGGTTCTGTGCTAAAACCACTTATCGTCTATGCACCAGCACTCGAAACGAAAAAGTATAATCCGT
ATTCTTTATTGACGAATGAAAGACATTCTTTTGAAGGGTATGAACCTCGAAACTATAACCGTGAATATTCGAAAGAAATA
ACGATGTACGATGCGATTTTAGAGTCAGCAAATGTACCAGCAGTTTCTTTATTAAATGAGTTAGGGGTAGAAGAAGGAAA
GCAATATTTAGAGAAAGGAAATGTTCACATTGCCGATGCTGGTTTAAGCACAGCGCTTGGCGGATTGAAAAATGGTGTTT
CAACATTTGATCTTGTGAAAATGTATCGTGCGTTTTTAGCAAATGGTGATATTATTGAGCCGCACGTTATTGATAAAGTG
TTAAATAGACACGGCGCAGTCATTGGAGAATCACCAAAAGTAGAAACGAAAATTTTCTCGAAACAAACGGCATGGTATAT
GACAAAAATGTTAGAAGGAGTTGTAAAGGAAGGGACAGCGAAAGCCGGTGTATATAGCGGAGCGTTAGCTGGGAAAACAG
GTACAACTTCCTTACCAAATGACGATAATGGGGCGAGGGATATGTGGTTCGTTGGCTATACGCCAAATTTAGTAGGCGCT
GTTTGGATTGGTTATGACCGCATAGATAAAGAGCATAAGTTACAAGGAGAAAGTGCGTCAGCAACAAAATTGTTTAAGAA
AATTTTAACGAAAGCAAATGTAGAACAGAAAGAGCAGTTTAGGAAACCAGAAGGTGTGGAAACAATCGGTGCTCCAATTC
GGCTGCGCAAGATTGAAGATGTGAAAATGAAATTAGCGTTTAGCCCTTTCGGTTTATTTAAAGCGAAATTAAGCTGGACA
CCACTTCCAGATGAAAGAATTATGTATCGTATTTATAGAGTGGAAAACGGAATTCATACGCACGTAGGTACTGTAAACGG
TGCCGGGGAATATGAGGAAAAGTTCGTTAACATCTTTTCAAAACCGAGTTTTTACGTTGTACCATATAACACACAAACGA
ATCGCGAAGGAGAAAAGTCAAAAGTAGCTAAACCATAG

Upstream 100 bases:

>100_bases
TATTATATCAACTCTCTTAAAAAACTTCGAAGATTATATACATAAAGATACTTCTTTTTCAAATAATAAATGTACCATCA
TTTGGAAAGAAGGCCGATGT

Downstream 100 bases:

>100_bases
TTTTTCTCTGTGTTATAATAAGAATGTTGTGAAAATAGTAAGCGTTTTTACGAATGTTTGTGTCAATTTAATGAACAACG
TACATAATATTATGCATTTT

Product: penicillin-binding protein 1

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 705; Mature: 705

Protein sequence:

>705_residues
MKKVKWTLLGGLATLVLAIVLYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYVENRDIVPIEQIPKYVQQSFIA
VEDSRFYEHQGIDYPSIFRALYKDTLAGEKVEGGSTITQQLAKNVFLTREKTFTRKLKEVAISLQLEQKYTKQQILEMYM
NHIYFGHGAYGIQAAAKLYFNKNVEDLTVEEGAMLAGLPKSPNGYSPYFSPEKSKERRDLVLSLMHKQGYLTAEESVRYQ
GKTIALYKNLDENELAYMPYIDMVIDEAARLYGLSHQEVLRGGYTFVVPMDEKIQKVAYNQFQDARNFPGKEEGAQGAFL
LMDNRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLIVYAPALETKKYNPYSLLTNERHSFEGYEPRNYNREYSKEI
TMYDAILESANVPAVSLLNELGVEEGKQYLEKGNVHIADAGLSTALGGLKNGVSTFDLVKMYRAFLANGDIIEPHVIDKV
LNRHGAVIGESPKVETKIFSKQTAWYMTKMLEGVVKEGTAKAGVYSGALAGKTGTTSLPNDDNGARDMWFVGYTPNLVGA
VWIGYDRIDKEHKLQGESASATKLFKKILTKANVEQKEQFRKPEGVETIGAPIRLRKIEDVKMKLAFSPFGLFKAKLSWT
PLPDERIMYRIYRVENGIHTHVGTVNGAGEYEEKFVNIFSKPSFYVVPYNTQTNREGEKSKVAKP

Sequences:

>Translated_705_residues
MKKVKWTLLGGLATLVLAIVLYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYVENRDIVPIEQIPKYVQQSFIA
VEDSRFYEHQGIDYPSIFRALYKDTLAGEKVEGGSTITQQLAKNVFLTREKTFTRKLKEVAISLQLEQKYTKQQILEMYM
NHIYFGHGAYGIQAAAKLYFNKNVEDLTVEEGAMLAGLPKSPNGYSPYFSPEKSKERRDLVLSLMHKQGYLTAEESVRYQ
GKTIALYKNLDENELAYMPYIDMVIDEAARLYGLSHQEVLRGGYTFVVPMDEKIQKVAYNQFQDARNFPGKEEGAQGAFL
LMDNRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLIVYAPALETKKYNPYSLLTNERHSFEGYEPRNYNREYSKEI
TMYDAILESANVPAVSLLNELGVEEGKQYLEKGNVHIADAGLSTALGGLKNGVSTFDLVKMYRAFLANGDIIEPHVIDKV
LNRHGAVIGESPKVETKIFSKQTAWYMTKMLEGVVKEGTAKAGVYSGALAGKTGTTSLPNDDNGARDMWFVGYTPNLVGA
VWIGYDRIDKEHKLQGESASATKLFKKILTKANVEQKEQFRKPEGVETIGAPIRLRKIEDVKMKLAFSPFGLFKAKLSWT
PLPDERIMYRIYRVENGIHTHVGTVNGAGEYEEKFVNIFSKPSFYVVPYNTQTNREGEKSKVAKP
>Mature_705_residues
MKKVKWTLLGGLATLVLAIVLYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYVENRDIVPIEQIPKYVQQSFIA
VEDSRFYEHQGIDYPSIFRALYKDTLAGEKVEGGSTITQQLAKNVFLTREKTFTRKLKEVAISLQLEQKYTKQQILEMYM
NHIYFGHGAYGIQAAAKLYFNKNVEDLTVEEGAMLAGLPKSPNGYSPYFSPEKSKERRDLVLSLMHKQGYLTAEESVRYQ
GKTIALYKNLDENELAYMPYIDMVIDEAARLYGLSHQEVLRGGYTFVVPMDEKIQKVAYNQFQDARNFPGKEEGAQGAFL
LMDNRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLIVYAPALETKKYNPYSLLTNERHSFEGYEPRNYNREYSKEI
TMYDAILESANVPAVSLLNELGVEEGKQYLEKGNVHIADAGLSTALGGLKNGVSTFDLVKMYRAFLANGDIIEPHVIDKV
LNRHGAVIGESPKVETKIFSKQTAWYMTKMLEGVVKEGTAKAGVYSGALAGKTGTTSLPNDDNGARDMWFVGYTPNLVGA
VWIGYDRIDKEHKLQGESASATKLFKKILTKANVEQKEQFRKPEGVETIGAPIRLRKIEDVKMKLAFSPFGLFKAKLSWT
PLPDERIMYRIYRVENGIHTHVGTVNGAGEYEEKFVNIFSKPSFYVVPYNTQTNREGEKSKVAKP

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=249, Percent_Identity=43.7751004016064, Blast_Score=195, Evalue=1e-50,
Organism=Escherichia coli, GI1786343, Length=566, Percent_Identity=28.7985865724382, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1788867, Length=537, Percent_Identity=27.0018621973929, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1789601, Length=180, Percent_Identity=36.6666666666667, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 79474; Mature: 79474

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVKWTLLGGLATLVLAIVLYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYVE
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEEECCCHHHHHCCCCEEHHEEC
NRDIVPIEQIPKYVQQSFIAVEDSRFYEHQGIDYPSIFRALYKDTLAGEKVEGGSTITQQ
CCCCCCHHHHHHHHHHHHEEEECCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
LAKNVFLTREKTFTRKLKEVAISLQLEQKYTKQQILEMYMNHIYFGHGAYGIQAAAKLYF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHH
NKNVEDLTVEEGAMLAGLPKSPNGYSPYFSPEKSKERRDLVLSLMHKQGYLTAEESVRYQ
CCCCCEEEECCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHCC
GKTIALYKNLDENELAYMPYIDMVIDEAARLYGLSHQEVLRGGYTFVVPMDEKIQKVAYN
CCEEEEEECCCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHCCCEEEEECHHHHHHHHHH
QFQDARNFPGKEEGAQGAFLLMDNRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLI
HHHHHHCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHH
VYAPALETKKYNPYSLLTNERHSFEGYEPRNYNREYSKEITMYDAILESANVPAVSLLNE
HCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
LGVEEGKQYLEKGNVHIADAGLSTALGGLKNGVSTFDLVKMYRAFLANGDIIEPHVIDKV
HCHHHHHHHHHHCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
LNRHGAVIGESPKVETKIFSKQTAWYMTKMLEGVVKEGTAKAGVYSGALAGKTGTTSLPN
HHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
DDNGARDMWFVGYTPNLVGAVWIGYDRIDKEHKLQGESASATKLFKKILTKANVEQKEQF
CCCCCCEEEEEECCCCCCEEEEECHHHCCHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHC
RKPEGVETIGAPIRLRKIEDVKMKLAFSPFGLFKAKLSWTPLPDERIMYRIYRVENGIHT
CCCCCCHHCCCCEEEEEECCEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEE
HVGTVNGAGEYEEKFVNIFSKPSFYVVPYNTQTNREGEKSKVAKP
ECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKVKWTLLGGLATLVLAIVLYKLIVLAGGYMMDEKQLVFHSSSRIVDQKGKEITKLYVE
CCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEEEECCCHHHHHCCCCEEHHEEC
NRDIVPIEQIPKYVQQSFIAVEDSRFYEHQGIDYPSIFRALYKDTLAGEKVEGGSTITQQ
CCCCCCHHHHHHHHHHHHEEEECCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHH
LAKNVFLTREKTFTRKLKEVAISLQLEQKYTKQQILEMYMNHIYFGHGAYGIQAAAKLYF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHH
NKNVEDLTVEEGAMLAGLPKSPNGYSPYFSPEKSKERRDLVLSLMHKQGYLTAEESVRYQ
CCCCCEEEECCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEHHHHHHCC
GKTIALYKNLDENELAYMPYIDMVIDEAARLYGLSHQEVLRGGYTFVVPMDEKIQKVAYN
CCEEEEEECCCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHCCCEEEEECHHHHHHHHHH
QFQDARNFPGKEEGAQGAFLLMDNRTGGIKAAIGGRKYVPRGFNRVFAKRQPGSVLKPLI
HHHHHHCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHH
VYAPALETKKYNPYSLLTNERHSFEGYEPRNYNREYSKEITMYDAILESANVPAVSLLNE
HCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
LGVEEGKQYLEKGNVHIADAGLSTALGGLKNGVSTFDLVKMYRAFLANGDIIEPHVIDKV
HCHHHHHHHHHHCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
LNRHGAVIGESPKVETKIFSKQTAWYMTKMLEGVVKEGTAKAGVYSGALAGKTGTTSLPN
HHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
DDNGARDMWFVGYTPNLVGAVWIGYDRIDKEHKLQGESASATKLFKKILTKANVEQKEQF
CCCCCCEEEEEECCCCCCEEEEECHHHCCHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHC
RKPEGVETIGAPIRLRKIEDVKMKLAFSPFGLFKAKLSWTPLPDERIMYRIYRVENGIHT
CCCCCCHHCCCCEEEEEECCEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEE
HVGTVNGAGEYEEKFVNIFSKPSFYVVPYNTQTNREGEKSKVAKP
ECCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]