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The map label for this gene is nrdE [H]

Identifier: 52143986

GI number: 52143986

Start: 1340323

End: 1341453

Strand: Direct

Name: nrdE [H]

Synonym: BCZK1242

Alternate gene names: 52143986

Gene position: 1340323-1341453 (Clockwise)

Preceding gene: 52143991

Following gene: 52143987

Centisome position: 25.28

GC content: 37.14

Gene sequence:

>1131_bases
TTGCGACACATTGAACTGAATAATGAAATCACGCAAATGCAGGACGGTTTTTATCAGCTTCATAAAGATAAAGAGGCATT
AGAAGTCTTTATGGAAGAAGCTAGAGAGAATACCGTTCATTTTAATAGCGTGGCAGAGCGAATGGAGTATATGAAAGAAC
ATGATTACTATTACAACGTTCTGGACGAGTATAACTTGGAGGAAGTAGAAGAGGTATATAACATCGCTTATGGTGAAAAC
TTCGAGTTCCAATCTTATATGGCAGCATCTAAGTTTTACAAAGATTATGCGTTAAAAACAAATGATCAAAAACAATACTT
AGAAAGCTATGAAGATCGTGTAGCAATTGTGTCATTATACTTAGGACGCGGTGATGTTGCGAAGGCAAAACAATTCGCAA
GCATGATTGTCAAACAAAACTACCAACCAGCGACACCAACCTTTTTAAATGCGGGAAGAAGCAGAAGAGGAGAAATGGTG
TCTTGTTTCTTGTTAGAGATGGATGATAGCTTAAATTCAATCGGCTTTAACATTAATACTGCAATGCAATTATCAAAAAT
CGGCGGAGGAGTAGCTTTAAACTTATCTAAGCTACGCGCACGCGGTGAGCAAATTAAAGGTATCGACAACGCTGCAAGTG
GTGTAGTACCTGTTATGAAATTACTTGAAGATTCGTTTTCATATGCGAATCAACTTGGCCAACGAAAAGGTGCTGGCGCA
GTATACTTAAACATTTTTCATTGGGATATTATTGAATTCCTTGATACGAAAAAAATAAATGCCGATGAGAAGAGCCGTAT
TCAGTCTCTATCAATCGGAATTATCGTTCCAAGTAAGTTCTTTGAGCTTGCTGAGAAAAACGAACCTTTCCATGTTTTCG
CGCCTTATACGGTGTATAAAGAATACGGAAAGCATTTAGATGACATTGATATCGATGAAATGTACGATGAATTAATGAGC
AATCCGAAAGTGAAGAAGAAGCCGCTAGATATTAGTGCACGTGATATGCTTATTAAAATTGCTATGATTCAGCTTGAGTC
GGGTTATCCGTACTTAATGTTTAAATCAAATGCAAATAATCAACATCCACTGAAGGATATTGGAACTGTGAAGATGTCGA
ACTTGTGTTAA

Upstream 100 bases:

>100_bases
GACAAAATTTCTGCTAAATATGAAGTGCCTATTGTATCAAAATTTGAGTTATCAGGAACAAATAACGATGTAGAATATTT
TAAGGAAAGGGTGCGGGAGA

Downstream 100 bases:

>100_bases
ATATGTAGCACCTTCTAGTAGAAATGCTAGTCGAAAACTCCGTTAAACGGGGAAAGCCTCAAATTTGAGGTAACCTACCG
TGCTAAATCTTATCTTAGAA

Product: ribonucleoside-diphosphate reductase, alpha subunit, N-terminal region

Products: NA

Alternate protein names: Ribonucleotide reductase large subunit [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MRHIELNNEITQMQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNVLDEYNLEEVEEVYNIAYGEN
FEFQSYMAASKFYKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMV
SCFLLEMDDSLNSIGFNINTAMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGA
VYLNIFHWDIIEFLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDIDEMYDELMS
NPKVKKKPLDISARDMLIKIAMIQLESGYPYLMFKSNANNQHPLKDIGTVKMSNLC

Sequences:

>Translated_376_residues
MRHIELNNEITQMQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNVLDEYNLEEVEEVYNIAYGEN
FEFQSYMAASKFYKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMV
SCFLLEMDDSLNSIGFNINTAMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGA
VYLNIFHWDIIEFLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDIDEMYDELMS
NPKVKKKPLDISARDMLIKIAMIQLESGYPYLMFKSNANNQHPLKDIGTVKMSNLC
>Mature_376_residues
MRHIELNNEITQMQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNVLDEYNLEEVEEVYNIAYGEN
FEFQSYMAASKFYKDYALKTNDQKQYLESYEDRVAIVSLYLGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMV
SCFLLEMDDSLNSIGFNINTAMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGA
VYLNIFHWDIIEFLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYKEYGKHLDDIDIDEMYDELMS
NPKVKKKPLDISARDMLIKIAMIQLESGYPYLMFKSNANNQHPLKDIGTVKMSNLC

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Homo sapiens, GI4506749, Length=307, Percent_Identity=29.3159609120521, Blast_Score=143, Evalue=3e-34,
Organism=Escherichia coli, GI1789030, Length=363, Percent_Identity=47.9338842975207, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI2367133, Length=240, Percent_Identity=22.5, Blast_Score=78, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17555242, Length=307, Percent_Identity=29.3159609120521, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322123, Length=390, Percent_Identity=25.6410256410256, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320914, Length=304, Percent_Identity=27.9605263157895, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17352481, Length=327, Percent_Identity=27.5229357798165, Blast_Score=135, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 43261; Mature: 43261

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHIELNNEITQMQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNV
CCEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCHHEEH
LDEYNLEEVEEVYNIAYGENFEFQSYMAASKFYKDYALKTNDQKQYLESYEDRVAIVSLY
HCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEE
LGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMVSCFLLEMDDSLNSIGFNINT
ECCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEEEEEEEECCCHHHCCCCCHH
AMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGA
HHHHHHCCCCEEEEHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VYLNIFHWDIIEFLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYK
EEEEEHHHHHHHHHHCCCCCCCHHHHHHHEEEEEEECHHHHHHHHCCCCEEEECCHHHHH
EYGKHLDDIDIDEMYDELMSNPKVKKKPLDISARDMLIKIAMIQLESGYPYLMFKSNANN
HHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEEEEECCCCCEEEEECCCCC
QHPLKDIGTVKMSNLC
CCCCHHHCCEECCCCC
>Mature Secondary Structure
MRHIELNNEITQMQDGFYQLHKDKEALEVFMEEARENTVHFNSVAERMEYMKEHDYYYNV
CCEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCHHEEH
LDEYNLEEVEEVYNIAYGENFEFQSYMAASKFYKDYALKTNDQKQYLESYEDRVAIVSLY
HCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEE
LGRGDVAKAKQFASMIVKQNYQPATPTFLNAGRSRRGEMVSCFLLEMDDSLNSIGFNINT
ECCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEEEEEEEECCCHHHCCCCCHH
AMQLSKIGGGVALNLSKLRARGEQIKGIDNAASGVVPVMKLLEDSFSYANQLGQRKGAGA
HHHHHHCCCCEEEEHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VYLNIFHWDIIEFLDTKKINADEKSRIQSLSIGIIVPSKFFELAEKNEPFHVFAPYTVYK
EEEEEHHHHHHHHHHCCCCCCCHHHHHHHEEEEEEECHHHHHHHHCCCCEEEECCHHHHH
EYGKHLDDIDIDEMYDELMSNPKVKKKPLDISARDMLIKIAMIQLESGYPYLMFKSNANN
HHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEEEEECCCCCEEEEECCCCC
QHPLKDIGTVKMSNLC
CCCCHHHCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969495; 9384377 [H]