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The map label for this gene is nrdE [H]

Identifier: 52143987

GI number: 52143987

Start: 1341938

End: 1342783

Strand: Direct

Name: nrdE [H]

Synonym: BCZK1243

Alternate gene names: 52143987

Gene position: 1341938-1342783 (Clockwise)

Preceding gene: 52143986

Following gene: 52143985

Centisome position: 25.32

GC content: 35.22

Gene sequence:

>846_bases
ATGGAAAACAAAGAAATTCGTGAAGCGGTTCATGCGGGAATGGAAGCTTTAACAGCTGTTTCTGATATGACGATTATTCC
GAATGCACCAACTGTGAAAAAAGCTAATGATGAGCTTCATTCAGTAGGGCTTGGCGCAATGAACTTACACGGATATTTAG
CAAAAAATAAAATCGCTTATGAAAGTGCAGAAGCGAAAGAATTCGCTCGTACATTCTTTATGATGTTAAATTACTACTCT
ATTGAGAAAAGTATGGAAATCGCTAAAGAAAAAGGCGAGACATTTAAAGACTTCGATAAATCTGATTATGCGAGCGGCAC
ATACTTTGAAAAGTATGAAACGACAGATTACAGTCCAGTAACTGAAAAAGTTCAGCAATTATTTGAAGGAATTCATATTC
CAACAAAAGAAGATTGGACAAGTTTAAAAGAGCAAGTACAGAAGAATGGTTTATATAACTCATATCGTCTTGCTATCGCT
CCGACACAATCAATCAGTTACGTTCAAAATGCAACTTCAAGCGTAATGCCGATCGTAAGTCAAATCGAATCAAGAACGTA
TGCGAATGCGACAACATATTATCCAATGCCGTATTTATCAAAAGATACGTTCTGGTACTATAAATCTTCTTACGATATGA
ATCAGTTTAAATTAATTGATTTAATCGCAGAAATTCAAGAACATATTGACCAAGGGATTAGTACAATTCTTTACGTTAAT
AGTGATATTTCAACACGTGAATTAGCACGTTACTACATCTATGCACATAAAAAAGGCTTAAAGAGTCTGTATTATACGAG
AACGCGTAAATTAAGCGTGGAAGAGTGTGTGGCTTGTACCGTTTAA

Upstream 100 bases:

>100_bases
CCAGCTTCAAGAAACTTCTGAAATAAATGATTACGGTACAGATGACATTATTCGTCGTGATATTAACTGTAACTTAGGAT
CGTTAAATATCGTAAATGTA

Downstream 100 bases:

>100_bases
TATAAAAAGTGAGACATGATGAGCAGGGTTTTCTGAACCCTGCTCATGCTTAGTTATCATATTTAGAGAAGGGGCGATTG
AATGCGTGCGGTAAACTGGA

Product: ribonucleoside-diphosphate reductase, alpha subunit, C-terminal region

Products: NA

Alternate protein names: Ribonucleotide reductase large subunit [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MENKEIREAVHAGMEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESAEAKEFARTFFMMLNYYS
IEKSMEIAKEKGETFKDFDKSDYASGTYFEKYETTDYSPVTEKVQQLFEGIHIPTKEDWTSLKEQVQKNGLYNSYRLAIA
PTQSISYVQNATSSVMPIVSQIESRTYANATTYYPMPYLSKDTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYVN
SDISTRELARYYIYAHKKGLKSLYYTRTRKLSVEECVACTV

Sequences:

>Translated_281_residues
MENKEIREAVHAGMEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESAEAKEFARTFFMMLNYYS
IEKSMEIAKEKGETFKDFDKSDYASGTYFEKYETTDYSPVTEKVQQLFEGIHIPTKEDWTSLKEQVQKNGLYNSYRLAIA
PTQSISYVQNATSSVMPIVSQIESRTYANATTYYPMPYLSKDTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYVN
SDISTRELARYYIYAHKKGLKSLYYTRTRKLSVEECVACTV
>Mature_281_residues
MENKEIREAVHAGMEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAYESAEAKEFARTFFMMLNYYS
IEKSMEIAKEKGETFKDFDKSDYASGTYFEKYETTDYSPVTEKVQQLFEGIHIPTKEDWTSLKEQVQKNGLYNSYRLAIA
PTQSISYVQNATSSVMPIVSQIESRTYANATTYYPMPYLSKDTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYVN
SDISTRELARYYIYAHKKGLKSLYYTRTRKLSVEECVACTV

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0209

COG function: function code F; Ribonucleotide reductase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase large chain family [H]

Homologues:

Organism=Escherichia coli, GI1789030, Length=289, Percent_Identity=40.1384083044983, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI2367133, Length=276, Percent_Identity=24.6376811594203, Blast_Score=82, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17352481, Length=299, Percent_Identity=23.4113712374582, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013346
- InterPro:   IPR013509
- InterPro:   IPR000788
- InterPro:   IPR008926
- InterPro:   IPR013554 [H]

Pfam domain/function: PF02867 Ribonuc_red_lgC; PF00317 Ribonuc_red_lgN; PF08343 RNR_N [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 32285; Mature: 32285

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00089 RIBORED_LARGE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKEIREAVHAGMEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAY
CCCHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCHHH
ESAEAKEFARTFFMMLNYYSIEKSMEIAKEKGETFKDFDKSDYASGTYFEKYETTDYSPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEECCCCCCCCHH
TEKVQQLFEGIHIPTKEDWTSLKEQVQKNGLYNSYRLAIAPTQSISYVQNATSSVMPIVS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
QIESRTYANATTYYPMPYLSKDTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYVN
HHHHHHHCCCEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEC
SDISTRELARYYIYAHKKGLKSLYYTRTRKLSVEECVACTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure
MENKEIREAVHAGMEALTAVSDMTIIPNAPTVKKANDELHSVGLGAMNLHGYLAKNKIAY
CCCHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCHHH
ESAEAKEFARTFFMMLNYYSIEKSMEIAKEKGETFKDFDKSDYASGTYFEKYETTDYSPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCEEECCCCCCCCHH
TEKVQQLFEGIHIPTKEDWTSLKEQVQKNGLYNSYRLAIAPTQSISYVQNATSSVMPIVS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
QIESRTYANATTYYPMPYLSKDTFWYYKSSYDMNQFKLIDLIAEIQEHIDQGISTILYVN
HHHHHHHCCCEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEC
SDISTRELARYYIYAHKKGLKSLYYTRTRKLSVEECVACTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969495; 9384377 [H]