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The map label for this gene is leuB [H]

Identifier: 52143943

GI number: 52143943

Start: 1379868

End: 1380932

Strand: Direct

Name: leuB [H]

Synonym: BCZK1286

Alternate gene names: 52143943

Gene position: 1379868-1380932 (Clockwise)

Preceding gene: 52143944

Following gene: 52143942

Centisome position: 26.03

GC content: 42.07

Gene sequence:

>1065_bases
TTGGAAAAACGTATTGTTTGTTTAGCTGGTGATGGTGTTGGCCCAGAAGTTATGGAAAGTGCGAAGGAAGTATTGCATAT
GGTAGAGAGGCTATATGGACATCATTTTCATTTGCAAGATGAGCATTTTGGTGGTGTTGCTATCGATTTAACGGGGCAAC
CATTACCACAACGAACACTTGCAGCTTGTTTAGCAAGTGATGCGGTTTTACTAGGCGCAGTTGGTGGACCAAGGTGGGAT
GGTGCGAAAGAAAGACCAGAGAAAGGATTATTAGCTTTAAGAAAAGGGCTTGGTGTATTTGCGAATGTTCGCCCTGTCAC
GGTAGAAAGTGAAACGGCACATTTATCACCATTAAAAAAGGCTGATGAAATTGATTTCGTTGTCGTTCGTGAATTAACAG
GTGGAATTTATTTTTCTTATCCGAAGGAACGAACGGACGAAGTAGCAACAGATACACTTACGTATCATCGCCATGAAATT
GAACGTATCGTTTCTTACGCCTTTCAATTAGCGAGCAAGCGGAAGAAAAAAGTAACATCTATTGATAAGGCGAATGTTTT
AGAATCTAGTAAACTATGGAGAACTGTAACAGAAGAAGTAGCACTTCGTTATCCAGATGTTGAACTAGAACATATTTTAG
TAGATGCGGCCGCTATGGAGCTAATTCGGAATCCAGGACGGTTTGATGTAATTGTAACAGAAAATTTATTTGGGGATATT
TTAAGTGACGAAGCTTCCGTATTAGCTGGATCATTAGGAATGCTTCCGTCAGCAAGTCACGCGGAAAAGGGACCTTCATT
ATACGAGCCTATTCACGGATCGGCACCGGATATTGCCGGGAAAAATAAGGCGAATCCAATTGCTATGATGCGCTCTGTTG
CCATGATGCTTGGGCAATCGTTCGGATTAACGAGAGAAGGGTGTGCAATTGAAGAAGCGATTTCAGCAGTCCTTAAATCA
GGGAAATGTACAGAAGATATCGGGGGGACTGAAACGACAACTTCATTTACGAAGGCAGTTATGCAAGAAATGGAAGAGCA
AGCGCTAGTAGGGAGAGGAAGATAA

Upstream 100 bases:

>100_bases
CTTTTATCCAGCTTGTGAAATAAGTGTTTGATAGGTGAGAGGAGCTATTCTCACCTATCAAATTAAAGTTCTGAAAATTC
AGTTAAAAGGAGTGTTGAAA

Downstream 100 bases:

>100_bases
TGGGGAAAAGGTTGCTAGATAAGCTTTGGGAAAAACACGTAGTTACGACAAATGAAAATGGATTAGATTTATTATATATC
GATCTGCACCTTGTTCATGA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEHFGGVAIDLTGQPLPQRTLAACLASDAVLLGAVGGPRWD
GAKERPEKGLLALRKGLGVFANVRPVTVESETAHLSPLKKADEIDFVVVRELTGGIYFSYPKERTDEVATDTLTYHRHEI
ERIVSYAFQLASKRKKKVTSIDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAAMELIRNPGRFDVIVTENLFGDI
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQSFGLTREGCAIEEAISAVLKS
GKCTEDIGGTETTTSFTKAVMQEMEEQALVGRGR

Sequences:

>Translated_354_residues
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEHFGGVAIDLTGQPLPQRTLAACLASDAVLLGAVGGPRWD
GAKERPEKGLLALRKGLGVFANVRPVTVESETAHLSPLKKADEIDFVVVRELTGGIYFSYPKERTDEVATDTLTYHRHEI
ERIVSYAFQLASKRKKKVTSIDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAAMELIRNPGRFDVIVTENLFGDI
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQSFGLTREGCAIEEAISAVLKS
GKCTEDIGGTETTTSFTKAVMQEMEEQALVGRGR
>Mature_354_residues
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEHFGGVAIDLTGQPLPQRTLAACLASDAVLLGAVGGPRWD
GAKERPEKGLLALRKGLGVFANVRPVTVESETAHLSPLKKADEIDFVVVRELTGGIYFSYPKERTDEVATDTLTYHRHEI
ERIVSYAFQLASKRKKKVTSIDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAAMELIRNPGRFDVIVTENLFGDI
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQSFGLTREGCAIEEAISAVLKS
GKCTEDIGGTETTTSFTKAVMQEMEEQALVGRGR

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=350, Percent_Identity=31.1428571428571, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI28178821, Length=351, Percent_Identity=31.0541310541311, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI28178816, Length=345, Percent_Identity=31.304347826087, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI4758582, Length=259, Percent_Identity=31.2741312741313, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI28178819, Length=194, Percent_Identity=34.5360824742268, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI28178838, Length=230, Percent_Identity=31.304347826087, Blast_Score=106, Evalue=4e-23,
Organism=Escherichia coli, GI87081683, Length=333, Percent_Identity=51.0510510510511, Blast_Score=318, Evalue=5e-88,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=35.3932584269663, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1787381, Length=373, Percent_Identity=23.8605898123324, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71986051, Length=354, Percent_Identity=31.638418079096, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17550882, Length=352, Percent_Identity=30.3977272727273, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI25144293, Length=354, Percent_Identity=27.4011299435028, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17505779, Length=347, Percent_Identity=27.0893371757925, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319830, Length=358, Percent_Identity=48.0446927374302, Blast_Score=290, Evalue=3e-79,
Organism=Saccharomyces cerevisiae, GI6322097, Length=357, Percent_Identity=36.9747899159664, Blast_Score=176, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6324709, Length=347, Percent_Identity=33.7175792507205, Blast_Score=155, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6324291, Length=353, Percent_Identity=29.4617563739377, Blast_Score=132, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24643268, Length=348, Percent_Identity=32.183908045977, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24643270, Length=348, Percent_Identity=32.183908045977, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI281362242, Length=352, Percent_Identity=30.6818181818182, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24648872, Length=352, Percent_Identity=30.6818181818182, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24661184, Length=359, Percent_Identity=28.133704735376, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI161078637, Length=348, Percent_Identity=27.0114942528736, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078633, Length=348, Percent_Identity=27.0114942528736, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24650122, Length=348, Percent_Identity=27.0114942528736, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078635, Length=348, Percent_Identity=27.0114942528736, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078639, Length=345, Percent_Identity=27.2463768115942, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI20130355, Length=342, Percent_Identity=24.2690058479532, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38494; Mature: 38494

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEHFGGVAIDLTGQPLPQRTL
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCCHHHH
AACLASDAVLLGAVGGPRWDGAKERPEKGLLALRKGLGVFANVRPVTVESETAHLSPLKK
HHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCEEEECCCCCCCCCCCC
ADEIDFVVVRELTGGIYFSYPKERTDEVATDTLTYHRHEIERIVSYAFQLASKRKKKVTS
CCCCCEEEEEEHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAAMELIRNPGRFDVIVTENLFGDI
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHH
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQS
HCCHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
FGLTREGCAIEEAISAVLKSGKCTEDIGGTETTTSFTKAVMQEMEEQALVGRGR
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEHFGGVAIDLTGQPLPQRTL
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCCHHHH
AACLASDAVLLGAVGGPRWDGAKERPEKGLLALRKGLGVFANVRPVTVESETAHLSPLKK
HHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCEEEECCCCCCCCCCCC
ADEIDFVVVRELTGGIYFSYPKERTDEVATDTLTYHRHEIERIVSYAFQLASKRKKKVTS
CCCCCEEEEEEHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAAMELIRNPGRFDVIVTENLFGDI
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHH
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQS
HCCHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
FGLTREGCAIEEAISAVLKSGKCTEDIGGTETTTSFTKAVMQEMEEQALVGRGR
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]