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The map label for this gene is leuC [H]

Identifier: 52143942

GI number: 52143942

Start: 1380932

End: 1382326

Strand: Direct

Name: leuC [H]

Synonym: BCZK1287

Alternate gene names: 52143942

Gene position: 1380932-1382326 (Clockwise)

Preceding gene: 52143943

Following gene: 161763542

Centisome position: 26.05

GC content: 41.72

Gene sequence:

>1395_bases
ATGGGGAAAAGGTTGCTAGATAAGCTTTGGGAAAAACACGTAGTTACGACAAATGAAAATGGATTAGATTTATTATATAT
CGATCTGCACCTTGTTCATGAAGTAACGTCACCGCAAGCCTTTGAAGGCTTGCGGCTTACAAATCGAAAGGTACGCAGAC
CAGATTTAACATTCGCAACGATGGATCATAATATTCCAACGAAAGATGTTTGGAATATTACCGATCGCATTGCGAAGCAG
CAACTAGATACACTTCGGGAAAATTGTAAACAATTTCAGGTGCCGTTAGCGGATATTGGAGATGAAGAGCAGGGGATCGT
TCATGTTATCGGGCCAGAACTTGGGCTCACGCAGCCAGGAAAAACAATTGTTTGTGGTGATAGTCATACAGCGACGCACG
GTGCGTTTGGTGCGCTAGCGTTTGGTATTGGTACGAGTGAAGTGGAACATGTATTGGCAACGCAAACGCTGTGGCAAAGA
AAACCGAAAGCGATGGGAATTGAACTAAAAGGAAAATTACAGAAAGGCGTTTACGCAAAAGATATTATTTTGCATCTCCT
TTCAAAGTACGGTGTAGCAGTTGGAACTGGATACGTAATGGAATTTTACGGAGAGACGATTGGGGCTATGGAGATGGAAG
AGAGAATGACGCTTTGTAATATGGCGATCGAAGGAGGAGCAAAAGCGGGTATTATCGCGCCAGATGAAAAAACATTTGCT
TATGTAAAAGGACGTAAATATGCACCGAGAGACTATGAAACTTTCGAGAAGAAATGGTCTGAACTTTATACAGATGCAGA
TGCAATTTATGATTTACATATTTCGATAGATGTTACAGATTTAGCGCCATACGTTACGTGGGGAACAAATCCGAGTATGG
GAGTTCGTATTGATGAGAAATTGCCAGAAAAACATGATGTAAATGACGAAAGAGCATTTTCTTATATGGGATTAAGCCCT
GGACAAAGTACGTATGATATTCCAGTTCAGCATGTCTTCATTGGATCTTGTACAAATTCCAGGCTATCTGATTTAGAAAT
TGCTGCAGCTGTTGTGAAAGGGAGAAAGGTAAAAGAAGGTGTGCGAGCACTCGTTGTACCTGGATCGAAAAGAGTAAGGG
ACGCGGCGATGCAAAAAGGACTACATCACATATTTGAAGAAGCAGGATTTGAATGGAGAGAACCTGGATGTTCCATGTGT
CTTGGAATGAATCCAGATCAAGTACCTGAAGGAGAGCATTGCGCTTCAACTTCAAATCGTAATTTTGAAGGAAGACAAGG
AAAAGGAGCACGAACGCATTTAGTTAGCCCAGCAATGGCGGCAGCAGCTGCGTTATATGGTCATTTTGTTGATACAAGAA
AGGAGAGTTATGATGGAGCCATTTCGTATTCATAA

Upstream 100 bases:

>100_bases
AATGTACAGAAGATATCGGGGGGACTGAAACGACAACTTCATTTACGAAGGCAGTTATGCAAGAAATGGAAGAGCAAGCG
CTAGTAGGGAGAGGAAGATA

Downstream 100 bases:

>100_bases
AGGTACTGCCGCAGTACTTATGAATGATAACATTGATACAGATCAAATTATTCCGAAGCAATATTTAAAGAGAATTGAAA
GAACTGGGTTTGGAAAGTTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MGKRLLDKLWEKHVVTTNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRKVRRPDLTFATMDHNIPTKDVWNITDRIAKQ
QLDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPGKTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQR
KPKAMGIELKGKLQKGVYAKDIILHLLSKYGVAVGTGYVMEFYGETIGAMEMEERMTLCNMAIEGGAKAGIIAPDEKTFA
YVKGRKYAPRDYETFEKKWSELYTDADAIYDLHISIDVTDLAPYVTWGTNPSMGVRIDEKLPEKHDVNDERAFSYMGLSP
GQSTYDIPVQHVFIGSCTNSRLSDLEIAAAVVKGRKVKEGVRALVVPGSKRVRDAAMQKGLHHIFEEAGFEWREPGCSMC
LGMNPDQVPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAALYGHFVDTRKESYDGAISYS

Sequences:

>Translated_464_residues
MGKRLLDKLWEKHVVTTNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRKVRRPDLTFATMDHNIPTKDVWNITDRIAKQ
QLDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPGKTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQR
KPKAMGIELKGKLQKGVYAKDIILHLLSKYGVAVGTGYVMEFYGETIGAMEMEERMTLCNMAIEGGAKAGIIAPDEKTFA
YVKGRKYAPRDYETFEKKWSELYTDADAIYDLHISIDVTDLAPYVTWGTNPSMGVRIDEKLPEKHDVNDERAFSYMGLSP
GQSTYDIPVQHVFIGSCTNSRLSDLEIAAAVVKGRKVKEGVRALVVPGSKRVRDAAMQKGLHHIFEEAGFEWREPGCSMC
LGMNPDQVPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAALYGHFVDTRKESYDGAISYS
>Mature_463_residues
GKRLLDKLWEKHVVTTNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRKVRRPDLTFATMDHNIPTKDVWNITDRIAKQQ
LDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPGKTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQRK
PKAMGIELKGKLQKGVYAKDIILHLLSKYGVAVGTGYVMEFYGETIGAMEMEERMTLCNMAIEGGAKAGIIAPDEKTFAY
VKGRKYAPRDYETFEKKWSELYTDADAIYDLHISIDVTDLAPYVTWGTNPSMGVRIDEKLPEKHDVNDERAFSYMGLSPG
QSTYDIPVQHVFIGSCTNSRLSDLEIAAAVVKGRKVKEGVRALVVPGSKRVRDAAMQKGLHHIFEEAGFEWREPGCSMCL
GMNPDQVPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAALYGHFVDTRKESYDGAISYS

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=354, Percent_Identity=26.5536723163842, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI8659555, Length=384, Percent_Identity=26.0416666666667, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI41352693, Length=368, Percent_Identity=26.3586956521739, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=58.494623655914, Blast_Score=523, Evalue=1e-150,
Organism=Escherichia coli, GI1787531, Length=363, Percent_Identity=28.3746556473829, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI87081781, Length=358, Percent_Identity=24.8603351955307, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI2367097, Length=337, Percent_Identity=24.9258160237389, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=359, Percent_Identity=30.6406685236769, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI32564738, Length=359, Percent_Identity=30.6406685236769, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=305, Percent_Identity=30.1639344262295, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=423, Percent_Identity=26.0047281323877, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=56.3559322033898, Blast_Score=499, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6320440, Length=414, Percent_Identity=28.743961352657, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6322261, Length=404, Percent_Identity=28.960396039604, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323335, Length=356, Percent_Identity=28.9325842696629, Blast_Score=133, Evalue=6e-32,
Organism=Drosophila melanogaster, GI281365315, Length=391, Percent_Identity=26.8542199488491, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI17864292, Length=391, Percent_Identity=26.8542199488491, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI161076999, Length=384, Percent_Identity=27.0833333333333, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI28571643, Length=391, Percent_Identity=27.8772378516624, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24645686, Length=380, Percent_Identity=28.1578947368421, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17137564, Length=373, Percent_Identity=24.9329758713137, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 51372; Mature: 51241

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKRLLDKLWEKHVVTTNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRKVRRPDLTFAT
CCHHHHHHHHHHCEEEECCCCCEEEEEEEEHHHHHCCCHHHCCCHHCCCCCCCCCCEEEE
MDHNIPTKDVWNITDRIAKQQLDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCCCC
KTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQRKPKAMGIELKGKLQKGVYAK
CEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECHHHCCCHHH
DIILHLLSKYGVAVGTGYVMEFYGETIGAMEMEERMTLCNMAIEGGAKAGIIAPDEKTFA
HHHHHHHHHCCHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEE
YVKGRKYAPRDYETFEKKWSELYTDADAIYDLHISIDVTDLAPYVTWGTNPSMGVRIDEK
EEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEHHCCCEEEECCCCCCCEEEHHH
LPEKHDVNDERAFSYMGLSPGQSTYDIPVQHVFIGSCTNSRLSDLEIAAAVVKGRKVKEG
CCCCCCCCHHHHEEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCHHHCC
VRALVVPGSKRVRDAAMQKGLHHIFEEAGFEWREPGCSMCLGMNPDQVPEGEHCASTSNR
CEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHCCCCCC
NFEGRQGKGARTHLVSPAMAAAAALYGHFVDTRKESYDGAISYS
CCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GKRLLDKLWEKHVVTTNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRKVRRPDLTFAT
CHHHHHHHHHHCEEEECCCCCEEEEEEEEHHHHHCCCHHHCCCHHCCCCCCCCCCEEEE
MDHNIPTKDVWNITDRIAKQQLDTLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCCCC
KTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQRKPKAMGIELKGKLQKGVYAK
CEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECHHHCCCHHH
DIILHLLSKYGVAVGTGYVMEFYGETIGAMEMEERMTLCNMAIEGGAKAGIIAPDEKTFA
HHHHHHHHHCCHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCEECCCCCEEE
YVKGRKYAPRDYETFEKKWSELYTDADAIYDLHISIDVTDLAPYVTWGTNPSMGVRIDEK
EEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEHHCCCEEEECCCCCCCEEEHHH
LPEKHDVNDERAFSYMGLSPGQSTYDIPVQHVFIGSCTNSRLSDLEIAAAVVKGRKVKEG
CCCCCCCCHHHHEEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCHHHCC
VRALVVPGSKRVRDAAMQKGLHHIFEEAGFEWREPGCSMCLGMNPDQVPEGEHCASTSNR
CEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHCCCCCC
NFEGRQGKGARTHLVSPAMAAAAALYGHFVDTRKESYDGAISYS
CCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA