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The map label for this gene is pcnB [H]

Identifier: 52143812

GI number: 52143812

Start: 1503656

End: 1504849

Strand: Direct

Name: pcnB [H]

Synonym: BCZK1419

Alternate gene names: 52143812

Gene position: 1503656-1504849 (Clockwise)

Preceding gene: 52143814

Following gene: 52143811

Centisome position: 28.37

GC content: 37.69

Gene sequence:

>1194_bases
ATGGAAAGATTTAAAAAAGCTAGTTCTATTATTGAGACTTTAAAGCAACAAGGGCATGAGGCTTACTTTGTCGGTGGAAG
CGTAAGAGACCTTATTATCGATAGGCCGATTGGAGATATTGATATTGCGACATCTGCTTTACCAGAAGAAGTGATGGCTA
TATTTCCAAGACATGTTCCTGTTGGTCTTGAACATGGAACTGTAATTGTAGTAGAAAATGGTGAGCCTTATGAGGTAACC
ACTTTTCGAACAGAGAGTGAATATGAAGATTTTCGAAGACCTAGTAGTGTTCAATTTGTTCGTTCATTAGAAGAGGATTT
AAAACGTCGTGATTTCACAATGAATGCAATTGCCATGACAGAAGAAGGCGAAAGGATCGATTTATTTGCAGGACAGGAAG
CGATTCAAAAGAGAGAAATTGTGACAGTGGGAACTGCTGCGGATCGTTTTCAAGAAGATGCTCTGCGAATGATGCGTGGT
ATTCGGTTCGTAAGTACGTTAGGTTTTTCTTTAGAGACGAAAACGAAACAAGCAATTGAAACATATGGACATTTGCTGGA
ACATATAGCAATTGAGAGAATTACAGTGGAGTTTGAAAAACTGTTAACTGGCACATATTGCGTGAAGGGCCTCAAAGAAT
TAGTGGAAACGAAACTATTTTCTCATCTGCCATATCTACAAATGTCAGAAGAGAGATTATTAAAAGCTACGCAGTATAAC
TGGGATTCTTTTGAAACAGACATTGAGGCATGGGCGTTTTTCTTATATTGTATTGGAGAAGAACATCCATCTGTCTTTTT
ACGTCAATGGAAGTTTTCAAATAAAAAAATAAAAGATATCGTCGCAGTTTTATTAACAATCCGTAAGAGAAAGGAGAAGG
ATTGGGATACAGTTCTTCTTTATAAAACGGGAATTCATATAGCTGAAATGGCAGAAAGAGTATATGAAGCGATGATTGAA
AGCTATGATCATACATCTGTTGAACGGGTACAAACGTTGTTTCAAGCATTGCCAATCAAAAGTCGACAAGAGATGGATGT
GACTGGGAATGATTTGTTAAACTGGGCAAGTAAAAAACCCGGTCCATGGGTTGCTGAAATGATTCAAAAGATTGAGGAAG
CAATCGTACAAGGAAACGTAGTTAATGAGAAAGAGTGTATAAGGGAGTGGCTGCAAGAATGCAATCTACTATAA

Upstream 100 bases:

>100_bases
GGAGAGCGAGCAAGAGAAAGTGTTTACGAGCAGTTTCGCTCTGAAAAAATCGTCTCACAATATGAAACGATTTACTATGA
TGTACTAAGGGATGACAAAA

Downstream 100 bases:

>100_bases
GAAAGCAGTTATTGCAAGTTTTTTCTGAGGCGGATGGCGAGTTTGTATCTGGCCAAACGATTAGTGATAAACTTGGTTGC
TCGAGAACCGCTGTATGGAA

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRHVPVGLEHGTVIVVENGEPYEVT
TFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMTEEGERIDLFAGQEAIQKREIVTVGTAADRFQEDALRMMRG
IRFVSTLGFSLETKTKQAIETYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKATQYN
WDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDTVLLYKTGIHIAEMAERVYEAMIE
SYDHTSVERVQTLFQALPIKSRQEMDVTGNDLLNWASKKPGPWVAEMIQKIEEAIVQGNVVNEKECIREWLQECNLL

Sequences:

>Translated_397_residues
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRHVPVGLEHGTVIVVENGEPYEVT
TFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMTEEGERIDLFAGQEAIQKREIVTVGTAADRFQEDALRMMRG
IRFVSTLGFSLETKTKQAIETYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKATQYN
WDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDTVLLYKTGIHIAEMAERVYEAMIE
SYDHTSVERVQTLFQALPIKSRQEMDVTGNDLLNWASKKPGPWVAEMIQKIEEAIVQGNVVNEKECIREWLQECNLL
>Mature_397_residues
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRHVPVGLEHGTVIVVENGEPYEVT
TFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMTEEGERIDLFAGQEAIQKREIVTVGTAADRFQEDALRMMRG
IRFVSTLGFSLETKTKQAIETYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKATQYN
WDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDTVLLYKTGIHIAEMAERVYEAMIE
SYDHTSVERVQTLFQALPIKSRQEMDVTGNDLLNWASKKPGPWVAEMIQKIEEAIVQGNVVNEKECIREWLQECNLL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=206, Percent_Identity=32.5242718446602, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI87081691, Length=230, Percent_Identity=31.7391304347826, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789436, Length=190, Percent_Identity=33.1578947368421, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71995920, Length=268, Percent_Identity=29.1044776119403, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI281360112, Length=269, Percent_Identity=29.7397769516729, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI21357337, Length=269, Percent_Identity=29.7397769516729, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45958; Mature: 45958

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRHVP
CCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC
VGLEHGTVIVVENGEPYEVTTFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMT
CCCCCCEEEEEECCCCEEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEC
EEGERIDLFAGQEAIQKREIVTVGTAADRFQEDALRMMRGIRFVSTLGFSLETKTKQAIE
CCCCEEEEECCHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
TYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKATQYN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHCC
WDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDTVLL
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
YKTGIHIAEMAERVYEAMIESYDHTSVERVQTLFQALPIKSRQEMDVTGNDLLNWASKKP
EECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCC
GPWVAEMIQKIEEAIVQGNVVNEKECIREWLQECNLL
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MERFKKASSIIETLKQQGHEAYFVGGSVRDLIIDRPIGDIDIATSALPEEVMAIFPRHVP
CCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC
VGLEHGTVIVVENGEPYEVTTFRTESEYEDFRRPSSVQFVRSLEEDLKRRDFTMNAIAMT
CCCCCCEEEEEECCCCEEEEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEC
EEGERIDLFAGQEAIQKREIVTVGTAADRFQEDALRMMRGIRFVSTLGFSLETKTKQAIE
CCCCEEEEECCHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
TYGHLLEHIAIERITVEFEKLLTGTYCVKGLKELVETKLFSHLPYLQMSEERLLKATQYN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHCC
WDSFETDIEAWAFFLYCIGEEHPSVFLRQWKFSNKKIKDIVAVLLTIRKRKEKDWDTVLL
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEE
YKTGIHIAEMAERVYEAMIESYDHTSVERVQTLFQALPIKSRQEMDVTGNDLLNWASKKP
EECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCC
GPWVAEMIQKIEEAIVQGNVVNEKECIREWLQECNLL
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA