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The map label for this gene is birA [H]

Identifier: 52143811

GI number: 52143811

Start: 1504834

End: 1505814

Strand: Direct

Name: birA [H]

Synonym: BCZK1420

Alternate gene names: 52143811

Gene position: 1504834-1505814 (Clockwise)

Preceding gene: 52143812

Following gene: 52143809

Centisome position: 28.39

GC content: 38.63

Gene sequence:

>981_bases
ATGCAATCTACTATAAGAAAGCAGTTATTGCAAGTTTTTTCTGAGGCGGATGGCGAGTTTGTATCTGGCCAAACGATTAG
TGATAAACTTGGTTGCTCGAGAACCGCTGTATGGAAACATATGGAGGACCTTCGGAATGAGGGCTACGAACTTGAAGCAG
TGCGTCGATTAGGTTATCGAATTGCAAGTAAGCCAGACAAAGTGACTGCGAATGAAATACAGTTAGGATTACAAACGAAA
CATATTGGTAGAACAGTGTATTTTGAAGAGTCTGTTGAATCAACGCAGCATATTGCAGCAAAGCTTGCTTATGAAGGTGC
AGAAGAAGGAACAATTGTTGTGGCAGAAGAACAAACAGCGGGTAGAGGTCGTTTAAGCAGAAAATGGCACTCTCCAAAAG
GAACGGGAATTTGGATGAGTATTATTTTACGTCCGTCAATCCCAGTTCATCATGCACCACAACTTACTTTGTTAGCCGCT
GTTAGCGTTGCGCAAGCAATTGAAAAATGCACAGGTGTAAACGTAGGAATAAAATGGCCGAATGATATTTTAATTCAAGG
AAAAAAGGCTGTAGGTATATTAACAGAAATGCAGGCTGATCCTGATAAAATTAATGCTGTCATTATGGGTATTGGTATTA
ATGCGAATCAAAAGCAAGAACATTTTGATGAGGAAATTCAGCATATTGCTACTTCTTTAGCGATTGAATCTGGTAAGCTA
ATTGTTCGTGCAGAGCTTATGCAACAAATCTTTTTACAACTAGAAAAATTATATGAAGAGTATTTACAAAATGGTTTCTC
TGTTATTAAAATTCTTTGGGAAAGTTACGCTGTAAGCATCGGGAAAGAAATTAACGCTCGAACGATGAGAGAGACGATTA
CGGGTGTAGCAAAAGGAATTACAGAGGATGGTGTATTGCTCCTAGAAGACCATGAAGGGAAAGTACATCATATTCATTCT
GCGGATATCGAAATTAAGTAA

Upstream 100 bases:

>100_bases
CCGGTCCATGGGTTGCTGAAATGATTCAAAAGATTGAGGAAGCAATCGTACAAGGAAACGTAGTTAATGAGAAAGAGTGT
ATAAGGGAGTGGCTGCAAGA

Downstream 100 bases:

>100_bases
AAGAAGTTCCTCTTTTGGAACTTCTTTTTTATTTCCATATTAATAAAATCTTTTAATATTTAAGTTTTATTTTAAAAGAT
CGTCCAGTTGAGTTTGCTTC

Product: biotin operon repressor

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MQSTIRKQLLQVFSEADGEFVSGQTISDKLGCSRTAVWKHMEDLRNEGYELEAVRRLGYRIASKPDKVTANEIQLGLQTK
HIGRTVYFEESVESTQHIAAKLAYEGAEEGTIVVAEEQTAGRGRLSRKWHSPKGTGIWMSIILRPSIPVHHAPQLTLLAA
VSVAQAIEKCTGVNVGIKWPNDILIQGKKAVGILTEMQADPDKINAVIMGIGINANQKQEHFDEEIQHIATSLAIESGKL
IVRAELMQQIFLQLEKLYEEYLQNGFSVIKILWESYAVSIGKEINARTMRETITGVAKGITEDGVLLLEDHEGKVHHIHS
ADIEIK

Sequences:

>Translated_326_residues
MQSTIRKQLLQVFSEADGEFVSGQTISDKLGCSRTAVWKHMEDLRNEGYELEAVRRLGYRIASKPDKVTANEIQLGLQTK
HIGRTVYFEESVESTQHIAAKLAYEGAEEGTIVVAEEQTAGRGRLSRKWHSPKGTGIWMSIILRPSIPVHHAPQLTLLAA
VSVAQAIEKCTGVNVGIKWPNDILIQGKKAVGILTEMQADPDKINAVIMGIGINANQKQEHFDEEIQHIATSLAIESGKL
IVRAELMQQIFLQLEKLYEEYLQNGFSVIKILWESYAVSIGKEINARTMRETITGVAKGITEDGVLLLEDHEGKVHHIHS
ADIEIK
>Mature_326_residues
MQSTIRKQLLQVFSEADGEFVSGQTISDKLGCSRTAVWKHMEDLRNEGYELEAVRRLGYRIASKPDKVTANEIQLGLQTK
HIGRTVYFEESVESTQHIAAKLAYEGAEEGTIVVAEEQTAGRGRLSRKWHSPKGTGIWMSIILRPSIPVHHAPQLTLLAA
VSVAQAIEKCTGVNVGIKWPNDILIQGKKAVGILTEMQADPDKINAVIMGIGINANQKQEHFDEEIQHIATSLAIESGKL
IVRAELMQQIFLQLEKLYEEYLQNGFSVIKILWESYAVSIGKEINARTMRETITGVAKGITEDGVLLLEDHEGKVHHIHS
ADIEIK

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Homo sapiens, GI46255045, Length=316, Percent_Identity=25, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1790408, Length=312, Percent_Identity=28.525641025641, Blast_Score=120, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24644246, Length=236, Percent_Identity=28.3898305084746, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 36251; Mature: 36251

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSTIRKQLLQVFSEADGEFVSGQTISDKLGCSRTAVWKHMEDLRNEGYELEAVRRLGYR
CCHHHHHHHHHHHHHCCCCEECCCCCHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
IASKPDKVTANEIQLGLQTKHIGRTVYFEESVESTQHIAAKLAYEGAEEGTIVVAEEQTA
HCCCCCCCCHHHHEECEEHHHCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
GRGRLSRKWHSPKGTGIWMSIILRPSIPVHHAPQLTLLAAVSVAQAIEKCTGVNVGIKWP
CCCCCHHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECC
NDILIQGKKAVGILTEMQADPDKINAVIMGIGINANQKQEHFDEEIQHIATSLAIESGKL
CCEEEECCHHHEEHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCE
IVRAELMQQIFLQLEKLYEEYLQNGFSVIKILWESYAVSIGKEINARTMRETITGVAKGI
EEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
TEDGVLLLEDHEGKVHHIHSADIEIK
CCCCEEEEECCCCCEEEEECCCCEEC
>Mature Secondary Structure
MQSTIRKQLLQVFSEADGEFVSGQTISDKLGCSRTAVWKHMEDLRNEGYELEAVRRLGYR
CCHHHHHHHHHHHHHCCCCEECCCCCHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
IASKPDKVTANEIQLGLQTKHIGRTVYFEESVESTQHIAAKLAYEGAEEGTIVVAEEQTA
HCCCCCCCCHHHHEECEEHHHCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
GRGRLSRKWHSPKGTGIWMSIILRPSIPVHHAPQLTLLAAVSVAQAIEKCTGVNVGIKWP
CCCCCHHCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECC
NDILIQGKKAVGILTEMQADPDKINAVIMGIGINANQKQEHFDEEIQHIATSLAIESGKL
CCEEEECCHHHEEHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCE
IVRAELMQQIFLQLEKLYEEYLQNGFSVIKILWESYAVSIGKEINARTMRETITGVAKGI
EEHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
TEDGVLLLEDHEGKVHHIHSADIEIK
CCCCEEEEECCCCCEEEEECCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]