Click here to switch to the map view.

The map label for this gene is emrB [C]

Identifier: 52143588

GI number: 52143588

Start: 1733771

End: 1735519

Strand: Direct

Name: emrB [C]

Synonym: BCZK1646

Alternate gene names: 52143588

Gene position: 1733771-1735519 (Clockwise)

Preceding gene: 52143589

Following gene: 52143587

Centisome position: 32.71

GC content: 32.76

Gene sequence:

>1749_bases
ATGGAACAATCAAAGGGTATTAAGATTGTCTTTTTAATGTGCTTAGGTATATTTCTTTGTATGATTGATACGACAATTAT
GAATATAGCTTTACCAGCGATACAAACGAGTGTAAATACTTCATTAGAAAAGATGTCTTGGGTATTAAATGTTTATACAA
TGACAATAGCTGTACTCGCTATTCCGCTAGGACGGATAGCTGATATTTTTGGGAAAGCAAAGATGTATATTCTCGGTTTG
ATAATTTTTGGTGGTGGATCAGTACTTTGTGCTTTTGCTAATACGGGCGATTTTCTTATCTTTTCTCGCTTTATACAAAG
TGTAGGTGCAGCGATTTTATTTCCAACTAGTATGGTAATTGGCGTATCAGCTATGCCATTAGCAAAGAGGCATGTTGCAC
TTGCGATTTTGGGAGTAACACAGGGACTATCAGCTGCGATGGGTCCAGTAATAGGTGGAATTATTACACAAAATTTCGGT
TGGAGATGGGTGTTCTTCGTAAATGTTCCAATTTGTATTCTTGGAATCATTTTATGCTGCATTATGTTACAAATAAAAAA
TGAAGAACGTATTATCTCAAAAATAGATTGGGTAGGACTACTATTAAGTAGCTTAGCAATTTTTTCTTTTACTCTCGTAT
TAATAAAAGGAAATACATGGGGATGGCAAAGTAACATAGCCTTGTCTTGTTATGCAATTAGCACTATTTCTCTTATTCTA
TTTGTTCTAGTGGAACGAAAGGTTCATAATCCAATGGTGAATTTAAATTTATTTCAAGATCGAATGTTTGTTGGAGCATC
CATCGTTGTTATATTAAGTAATGTATTTTTAATTGGAGTTACTGTATTACTTCCAACGTTCTTAACGAAAATACAAGGTC
GAACGGAAATTGAAGCAGCCTTTTTAGTGACACCTATTTCAGCGATGATATTTTTTGTCTCACCAATTGCAGCAACATTA
ATTAAAAAGCTTGGCAAAGTAACTATTATTTTGTCAGGATTTCTTGTTATGGGTCTATCTTATTATTGGTTGCAAATGAT
TGATGTTAATTCAACAAATATAGAAATTATTATTCCATGTATGATATTAGGTGTTGGATATGGTTTAGTAGTGGGGCCAA
TTACAGTTCTAAGTGCATCTTCGTTTGAAGGGGAATTATTAACTGCTTCTCAAAGTGTTGTATCAATGTTGCGGCAAGTA
GGTATTGTTTTAGCGGTCGCCATATTTGTATCTAACTTAACTCATAATTTAACTATAAATAAAGAGAATGTATATCGTTA
TGCAGAAGAAAAAGTTCGTAATATTCCTGTGAGTAGTGCCGAGCAAGCGGAGATATTACAAGTAACAAAGGAAAAAATAG
AGAATCAAAGTATAGAAACAAATGTAGAAAAGCATCAAAATGAAACATTTGTAGGGTTACATAAAGAGAAAAAAGACGAA
TTAATTCACCAAAAAGTAGATAAAGTATTAAGAGAAGTTCCAGTAGAATATAGAGATGTAAAAAGAGAAGAAGTTATGAG
TCAAGTGACAAATGAAGTTGAAAAACAAGAAGAAAGCATAAAGAAAGAAGTACTCGCATTTTCGAATGATGTACATCATT
ATGCTAAGAATCAGATGGCGATGAGTTTTACAGATTTATATAAAGCGAGTGTGCCAATTATTTTAATTTGTGCTTTAGTA
AGTTTGTTATTTTCGGAAAGAAAGGTTTTGAGTAAAAAGAAAAGTGGAAGAGTAGTAGAGGAAGTGTAA

Upstream 100 bases:

>100_bases
TAGAGACTTTGAAAAGAAAACTAGGGCAACTAGAAGCAAAAGAACACAATAAAATGGAAGAGTGAAAAAACGTTCATAAT
AAATAAAGGGGTAGAAGAAC

Downstream 100 bases:

>100_bases
AGAAAACAGGCTAATAGCAGCCTGTTTTTTTGTTTTCCCCTTACATATTGTGAATAGTAATATTTTTAATATAGCATAAG
AGAAGTCGAGTTTGGTTAGA

Product: multidrug ABC transporter, permease

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MEQSKGIKIVFLMCLGIFLCMIDTTIMNIALPAIQTSVNTSLEKMSWVLNVYTMTIAVLAIPLGRIADIFGKAKMYILGL
IIFGGGSVLCAFANTGDFLIFSRFIQSVGAAILFPTSMVIGVSAMPLAKRHVALAILGVTQGLSAAMGPVIGGIITQNFG
WRWVFFVNVPICILGIILCCIMLQIKNEERIISKIDWVGLLLSSLAIFSFTLVLIKGNTWGWQSNIALSCYAISTISLIL
FVLVERKVHNPMVNLNLFQDRMFVGASIVVILSNVFLIGVTVLLPTFLTKIQGRTEIEAAFLVTPISAMIFFVSPIAATL
IKKLGKVTIILSGFLVMGLSYYWLQMIDVNSTNIEIIIPCMILGVGYGLVVGPITVLSASSFEGELLTASQSVVSMLRQV
GIVLAVAIFVSNLTHNLTINKENVYRYAEEKVRNIPVSSAEQAEILQVTKEKIENQSIETNVEKHQNETFVGLHKEKKDE
LIHQKVDKVLREVPVEYRDVKREEVMSQVTNEVEKQEESIKKEVLAFSNDVHHYAKNQMAMSFTDLYKASVPIILICALV
SLLFSERKVLSKKKSGRVVEEV

Sequences:

>Translated_582_residues
MEQSKGIKIVFLMCLGIFLCMIDTTIMNIALPAIQTSVNTSLEKMSWVLNVYTMTIAVLAIPLGRIADIFGKAKMYILGL
IIFGGGSVLCAFANTGDFLIFSRFIQSVGAAILFPTSMVIGVSAMPLAKRHVALAILGVTQGLSAAMGPVIGGIITQNFG
WRWVFFVNVPICILGIILCCIMLQIKNEERIISKIDWVGLLLSSLAIFSFTLVLIKGNTWGWQSNIALSCYAISTISLIL
FVLVERKVHNPMVNLNLFQDRMFVGASIVVILSNVFLIGVTVLLPTFLTKIQGRTEIEAAFLVTPISAMIFFVSPIAATL
IKKLGKVTIILSGFLVMGLSYYWLQMIDVNSTNIEIIIPCMILGVGYGLVVGPITVLSASSFEGELLTASQSVVSMLRQV
GIVLAVAIFVSNLTHNLTINKENVYRYAEEKVRNIPVSSAEQAEILQVTKEKIENQSIETNVEKHQNETFVGLHKEKKDE
LIHQKVDKVLREVPVEYRDVKREEVMSQVTNEVEKQEESIKKEVLAFSNDVHHYAKNQMAMSFTDLYKASVPIILICALV
SLLFSERKVLSKKKSGRVVEEV
>Mature_582_residues
MEQSKGIKIVFLMCLGIFLCMIDTTIMNIALPAIQTSVNTSLEKMSWVLNVYTMTIAVLAIPLGRIADIFGKAKMYILGL
IIFGGGSVLCAFANTGDFLIFSRFIQSVGAAILFPTSMVIGVSAMPLAKRHVALAILGVTQGLSAAMGPVIGGIITQNFG
WRWVFFVNVPICILGIILCCIMLQIKNEERIISKIDWVGLLLSSLAIFSFTLVLIKGNTWGWQSNIALSCYAISTISLIL
FVLVERKVHNPMVNLNLFQDRMFVGASIVVILSNVFLIGVTVLLPTFLTKIQGRTEIEAAFLVTPISAMIFFVSPIAATL
IKKLGKVTIILSGFLVMGLSYYWLQMIDVNSTNIEIIIPCMILGVGYGLVVGPITVLSASSFEGELLTASQSVVSMLRQV
GIVLAVAIFVSNLTHNLTINKENVYRYAEEKVRNIPVSSAEQAEILQVTKEKIENQSIETNVEKHQNETFVGLHKEKKDE
LIHQKVDKVLREVPVEYRDVKREEVMSQVTNEVEKQEESIKKEVLAFSNDVHHYAKNQMAMSFTDLYKASVPIILICALV
SLLFSERKVLSKKKSGRVVEEV

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=469, Percent_Identity=27.9317697228145, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1788710, Length=391, Percent_Identity=29.156010230179, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1790195, Length=404, Percent_Identity=25.7425742574257, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI87081983, Length=359, Percent_Identity=24.2339832869081, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1788392, Length=279, Percent_Identity=26.1648745519713, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322958, Length=234, Percent_Identity=31.1965811965812, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321663, Length=131, Percent_Identity=33.587786259542, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6325455, Length=237, Percent_Identity=25.3164556962025, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324954, Length=305, Percent_Identity=20.655737704918, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323735, Length=315, Percent_Identity=27.6190476190476, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319783, Length=180, Percent_Identity=30.5555555555556, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 64325; Mature: 64325

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQSKGIKIVFLMCLGIFLCMIDTTIMNIALPAIQTSVNTSLEKMSWVLNVYTMTIAVLA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPLGRIADIFGKAKMYILGLIIFGGGSVLCAFANTGDFLIFSRFIQSVGAAILFPTSMVI
HHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHH
GVSAMPLAKRHVALAILGVTQGLSAAMGPVIGGIITQNFGWRWVFFVNVPICILGIILCC
CCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHH
IMLQIKNEERIISKIDWVGLLLSSLAIFSFTLVLIKGNTWGWQSNIALSCYAISTISLIL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCEEEHHHHHHHHHHH
FVLVERKVHNPMVNLNLFQDRMFVGASIVVILSNVFLIGVTVLLPTFLTKIQGRTEIEAA
HHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
FLVTPISAMIFFVSPIAATLIKKLGKVTIILSGFLVMGLSYYWLQMIDVNSTNIEIIIPC
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEHHH
MILGVGYGLVVGPITVLSASSFEGELLTASQSVVSMLRQVGIVLAVAIFVSNLTHNLTIN
HHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
KENVYRYAEEKVRNIPVSSAEQAEILQVTKEKIENQSIETNVEKHQNETFVGLHKEKKDE
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHCCHHHCCCCCEEECCHHHHHH
LIHQKVDKVLREVPVEYRDVKREEVMSQVTNEVEKQEESIKKEVLAFSNDVHHYAKNQMA
HHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSFTDLYKASVPIILICALVSLLFSERKVLSKKKSGRVVEEV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCC
>Mature Secondary Structure
MEQSKGIKIVFLMCLGIFLCMIDTTIMNIALPAIQTSVNTSLEKMSWVLNVYTMTIAVLA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IPLGRIADIFGKAKMYILGLIIFGGGSVLCAFANTGDFLIFSRFIQSVGAAILFPTSMVI
HHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHH
GVSAMPLAKRHVALAILGVTQGLSAAMGPVIGGIITQNFGWRWVFFVNVPICILGIILCC
CCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHH
IMLQIKNEERIISKIDWVGLLLSSLAIFSFTLVLIKGNTWGWQSNIALSCYAISTISLIL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCEEEHHHHHHHHHHH
FVLVERKVHNPMVNLNLFQDRMFVGASIVVILSNVFLIGVTVLLPTFLTKIQGRTEIEAA
HHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
FLVTPISAMIFFVSPIAATLIKKLGKVTIILSGFLVMGLSYYWLQMIDVNSTNIEIIIPC
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEHHH
MILGVGYGLVVGPITVLSASSFEGELLTASQSVVSMLRQVGIVLAVAIFVSNLTHNLTIN
HHHHHCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
KENVYRYAEEKVRNIPVSSAEQAEILQVTKEKIENQSIETNVEKHQNETFVGLHKEKKDE
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHCCHHHCCCCCEEECCHHHHHH
LIHQKVDKVLREVPVEYRDVKREEVMSQVTNEVEKQEESIKKEVLAFSNDVHHYAKNQMA
HHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSFTDLYKASVPIILICALVSLLFSERKVLSKKKSGRVVEEV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]