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The map label for this gene is fosB [H]

Identifier: 52143387

GI number: 52143387

Start: 1974343

End: 1974759

Strand: Direct

Name: fosB [H]

Synonym: BCZK1847

Alternate gene names: 52143387

Gene position: 1974343-1974759 (Clockwise)

Preceding gene: 52143396

Following gene: 52143386

Centisome position: 37.25

GC content: 32.13

Gene sequence:

>417_bases
TTGTTAAAAGGAATCAATCATCTTTGTTTTTCAGTATCTAATTTAGAAGATTCTATTACATTTTACGAAAAAGTATTGGA
AGGAGAATTATTAGTTAGAGGAAGAAAACTTGCATATTTTAATATATGTGGAGTATGGATAGCGCTTAATGAAGAAATAC
ATATTCCAAGAAACGAGATTCATCAATCTTATACACATATTGCGTTTTCTGTTGAACAGAAAGACTTTGAACGTCTACTG
CAGCGATTAGAAGAAAATGATGTTCATATTTTACAAGGAAGAGAACGGGATGTAAGGGACTGTGAATCTATATATTTTGT
TGATCCTGACGGCCACAAGTTTGAGTTCCATTCAGGGACATTGCAAGACAGGCTTAATTATTATAGGGAAGATAAACCTC
ATATGACGTTTTATTAA

Upstream 100 bases:

>100_bases
GGGGACTTCAGTTATAAATGGGCATATATTACAAAAAAATAGTACAATAAAAGATGAGTGAATTTCCGCTCATCTTTTAT
TTGTATAGGAGGAATTAGAG

Downstream 100 bases:

>100_bases
GGGGGCAAAGAAAGTTGCATGCGCTAGTAATTGGTGGAACAGGAATGCTAAAGAGGGTTTCTATATGGCTTTGTGAGCAA
GGGTTTCATGTTTCTATTAT

Product: fosfomycin resistance protein FosB

Products: NA

Alternate protein names: Fosfomycin resistance protein 1 [H]

Number of amino acids: Translated: 138; Mature: 138

Protein sequence:

>138_residues
MLKGINHLCFSVSNLEDSITFYEKVLEGELLVRGRKLAYFNICGVWIALNEEIHIPRNEIHQSYTHIAFSVEQKDFERLL
QRLEENDVHILQGRERDVRDCESIYFVDPDGHKFEFHSGTLQDRLNYYREDKPHMTFY

Sequences:

>Translated_138_residues
MLKGINHLCFSVSNLEDSITFYEKVLEGELLVRGRKLAYFNICGVWIALNEEIHIPRNEIHQSYTHIAFSVEQKDFERLL
QRLEENDVHILQGRERDVRDCESIYFVDPDGHKFEFHSGTLQDRLNYYREDKPHMTFY
>Mature_138_residues
MLKGINHLCFSVSNLEDSITFYEKVLEGELLVRGRKLAYFNICGVWIALNEEIHIPRNEIHQSYTHIAFSVEQKDFERLL
QRLEENDVHILQGRERDVRDCESIYFVDPDGHKFEFHSGTLQDRLNYYREDKPHMTFY

Specific function: Metallothiol transferase which confers resistance to fosfomycin by catalyzing the addition of a thiol cofactor to fosfomycin. L-cysteine is probably the physiological thiol donor [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fosfomycin resistance protein family. FosB subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR022858 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: NA

Molecular weight: Translated: 16510; Mature: 16510

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKGINHLCFSVSNLEDSITFYEKVLEGELLVRGRKLAYFNICGVWIALNEEIHIPRNEI
CCCCCCHHHEECCCCHHHHHHHHHHHCCCEEECCCEEEEEEEEEEEEEECCEEECCHHHH
HQSYTHIAFSVEQKDFERLLQRLEENDVHILQGRERDVRDCESIYFVDPDGHKFEFHSGT
HHHHHHEEEEECHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCCCEEEECCCC
LQDRLNYYREDKPHMTFY
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLKGINHLCFSVSNLEDSITFYEKVLEGELLVRGRKLAYFNICGVWIALNEEIHIPRNEI
CCCCCCHHHEECCCCHHHHHHHHHHHCCCEEECCCEEEEEEEEEEEEEECCEEECCHHHH
HQSYTHIAFSVEQKDFERLLQRLEENDVHILQGRERDVRDCESIYFVDPDGHKFEFHSGT
HHHHHHEEEEECHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCCCEEEECCCC
LQDRLNYYREDKPHMTFY
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA