Click here to switch to the map view.

The map label for this gene is rhtB [H]

Identifier: 52143121

GI number: 52143121

Start: 2238737

End: 2239369

Strand: Reverse

Name: rhtB [H]

Synonym: BCZK2117

Alternate gene names: 52143121

Gene position: 2239369-2238737 (Counterclockwise)

Preceding gene: 52143120

Following gene: 52143134

Centisome position: 42.24

GC content: 32.07

Gene sequence:

>633_bases
ATGATTGAAAATTATTTTCTTTTCATCATTATGTCTATTTGTCTTATTATTTTACCCGGCCCTGATACTGCAATGGCTAC
GAAAAACACATTAGTTGCTGGTAAAGTGGGCGGGGTGAAAACAGTGTTCGGTACATGTATTGCACTATTGATCCATACTT
TAGCAGCAGTAATCGGCCTTTCTGCACTTATTGTAAAGTCAGCTCTTTTATTTTCTATTTTCAAGTACGTTGGAGCTCTA
TATTTAATTTATATTGGTATTAAAGCCCTTTTAGCAGTAAGAAATAAAGAAGGGGTCGATACGAACGATATTTCTTTGCA
TAATGAAGACGAACACACTTCTTGCTTTCGTCAAGGGTTTCTTACGAATTTATTAAACCCTAAAGTTGCAGTCTTCTTTT
TAACATTTTTACCGCAATTTTTAAATCCAAATCATAATACGTTTATCCAACTTCTCGTAATGGGCCTTACTTACCTCATT
TTAACAGTTATATGGTTCGCTTTTTATATATTTTTAATTGATAAAATTAGTGCTTTTATGAAAAAATCGAAGACACAACG
TTATATTCAAGGGTTAACAGGAATTGTCTTAATTGGTTTTGGAATTAAACTAGCTTTTGAAAAAAGCAATTAA

Upstream 100 bases:

>100_bases
TTATTTAATATTTTTTAATAAAATTTAATTTTATTAAATCGAAATGTGTGCTACACTATTTTTAATCAAATATTCTAAAA
ATAAAAAAGGAGGAGCGACT

Downstream 100 bases:

>100_bases
CAAGAAGAAAACCACCACAATTAATAGTGGTGGTTTTCATTTAATAATTGATTTGGCTGTCTACTTTATATTAAAACCAA
CGCTCAGTTACAACTTTTTT

Product: homoserine/threonine efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MIENYFLFIIMSICLIILPGPDTAMATKNTLVAGKVGGVKTVFGTCIALLIHTLAAVIGLSALIVKSALLFSIFKYVGAL
YLIYIGIKALLAVRNKEGVDTNDISLHNEDEHTSCFRQGFLTNLLNPKVAVFFLTFLPQFLNPNHNTFIQLLVMGLTYLI
LTVIWFAFYIFLIDKISAFMKKSKTQRYIQGLTGIVLIGFGIKLAFEKSN

Sequences:

>Translated_210_residues
MIENYFLFIIMSICLIILPGPDTAMATKNTLVAGKVGGVKTVFGTCIALLIHTLAAVIGLSALIVKSALLFSIFKYVGAL
YLIYIGIKALLAVRNKEGVDTNDISLHNEDEHTSCFRQGFLTNLLNPKVAVFFLTFLPQFLNPNHNTFIQLLVMGLTYLI
LTVIWFAFYIFLIDKISAFMKKSKTQRYIQGLTGIVLIGFGIKLAFEKSN
>Mature_210_residues
MIENYFLFIIMSICLIILPGPDTAMATKNTLVAGKVGGVKTVFGTCIALLIHTLAAVIGLSALIVKSALLFSIFKYVGAL
YLIYIGIKALLAVRNKEGVDTNDISLHNEDEHTSCFRQGFLTNLLNPKVAVFFLTFLPQFLNPNHNTFIQLLVMGLTYLI
LTVIWFAFYIFLIDKISAFMKKSKTQRYIQGLTGIVLIGFGIKLAFEKSN

Specific function: Conducts The Efflux Of Homoserine And Homoserine Lactone. [C]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=207, Percent_Identity=27.536231884058, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1788099, Length=208, Percent_Identity=27.4038461538462, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI48994978, Length=199, Percent_Identity=25.1256281407035, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1786522, Length=189, Percent_Identity=22.2222222222222, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23322; Mature: 23322

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIENYFLFIIMSICLIILPGPDTAMATKNTLVAGKVGGVKTVFGTCIALLIHTLAAVIGL
CCCHHHHHHHHHHHHHEECCCCCHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
SALIVKSALLFSIFKYVGALYLIYIGIKALLAVRNKEGVDTNDISLHNEDEHTSCFRQGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
LTNLLNPKVAVFFLTFLPQFLNPNHNTFIQLLVMGLTYLILTVIWFAFYIFLIDKISAFM
HHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKSKTQRYIQGLTGIVLIGFGIKLAFEKSN
HHHHHHHHHHHHHHHHHHHHHHEEEEECCC
>Mature Secondary Structure
MIENYFLFIIMSICLIILPGPDTAMATKNTLVAGKVGGVKTVFGTCIALLIHTLAAVIGL
CCCHHHHHHHHHHHHHEECCCCCHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
SALIVKSALLFSIFKYVGALYLIYIGIKALLAVRNKEGVDTNDISLHNEDEHTSCFRQGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
LTNLLNPKVAVFFLTFLPQFLNPNHNTFIQLLVMGLTYLILTVIWFAFYIFLIDKISAFM
HHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKSKTQRYIQGLTGIVLIGFGIKLAFEKSN
HHHHHHHHHHHHHHHHHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 9308178 [H]